Plasmids: a practical approach
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Oxford [u.a.]
IRL Press
1993
|
| Ausgabe: | 2. ed. |
| Schriftenreihe: | The practical approach series
138 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Literaturangaben |
| Beschreibung: | XVIII, 252 S. Ill., graph. Darst. |
| ISBN: | 0199634459 0199634440 |
Internformat
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| 245 | 1 | 0 | |a Plasmids |b a practical approach |c ed. by K. G. Hardy |
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Datensatz im Suchindex
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| adam_text | IMAGE 1
CONTENTS
LIST OF CONTRIBUTORS XV
LIST OF ABBREVIATIONS XVII
1. PLASMID REPLICATION AND MAINTENANCE 1
KURT NORDSTROM 1. INTRODUCTION 1
2. SINGLE-CELL RESISTANCE (SCR) TO ANTIBODIOTICS 1
3. DETERMINATION OF PLASMID COPY NUMBERS 4
COPY NUMBERS 4
AMOUNT OF PLASMID DNA 5
COPY (COP) MUTANTS 7
4. PARTITION AND OTHER MAINTENANCE FUNCTIONS 8
PLASMID PARTITION 8
KILLER SYSTEMS 12
SYSTEMS THAT DELAY CELL DIVISION 12
SITE-SPECIFIC RECOMBINATION SYSTEMS 12
DISTINCTION BETWEEN DIFFERENT MAINTENANCE SYSTEMS 12
5. INCOMPATIBILITY 13
INCOMPATIBILITY CAUSED BY RANDOMIZATION DURING REPLICATION 13
INCOMPATIBILITY CAUSED BY THE PARTITION PROCESS 20
INCOMPATIBILITY CAUSED BY KILLER SYSTEMS 21
COMPARISON BETWEEN DIFFERENT INCOMPATIBILITY GENES 21
6. BASIC REPLICONS 21
IDENTIFICATION OF REPLICONS BY DELETION ANALYSIS 21
IDENTIFICATION OF REPLICONS BY CLONING 21
IDENTIFICATION BY THE FORMATION OF COINTEGRATES 22
IDENTIFICATION OF REPLICONS BY INCOMPATIBILITY TYPING 22 IDENTIFICATION
OF REPLICONS BY REPLICON-SPECIFIC PROBES 22 MODE OF REPLICATION 22
7. ORIGINS (ORI) OF REPLICATION 23
IDENTIFICATION OF ORIGINS 23
DIRECTIONALITY OF REPLICATION 25
INITIATION OF UNIDIRECTIONAL REPLICATION 25
ROLLING CIRCLE REPLICATION 26
8. REP FUNCTIONS 26
IDENTIFICATION 26
CISLTRANS ACTIVITY 27
IMAGE 2
CONTENTS
9. COP FUNCTIONS IDENTIFICATION OF COP GENES BY CLONING CHARACTERIZATION
OF COP GENES RUNAWAY REPLICATION KINETICS OF COPY NUMBER CONTROL
10. REPLICATIVE INTERMEDIATES
11. TIMING OF REPLICATION THE BABY MACHINE MESELSON-STAHL DENSITY SHIFT
EXPERIMENTS ANALYSIS OF DNA REPLICATION IN CELLS OF DIFFERENT SIZE
WHAT DO THE METHODS ACTUALLY MEASURE?
12. IN VITRO REPLICATION
13. VISUALIZATION OF PLASMIDS EM FLUORESCENCE MICROSCOPY
ACKNOWLEDGEMENTS
REFERENCES
27 28 29 29 29
31
32 32 32 32 33
33
35 35 35
35
36
2. PLASMIDS FROM GRAM-POSITIVE BACTERIA 39
JUAN C. ALONSO AND MANUEL ESPINOSA 1. INTRODUCTION 39
2. CLASSIFICATION 40
INCOMPATIBILITY GROUPING 42
INCOMPATIBILITY TEST 44
3. ISOLATION OF PLASMID DNA 46
4. HOST RANGE AND PLASMID TRANSFER 48
TRANSFORMATION OF COMPETENT CELLS 49
TRANSDUCTION 49
CONJUGATION 51
ELECTROPORATION 52
TRANSFORMATION OF PROTOPLASTS 53
5. PLASMID REPLICATION MODES 55
ANALYSIS OF THE PLASMID REPLICATION MODE 56
ACKNOWLEDGEMENTS 60
REFERENCES 62
3. LACTOCOCCAL PLASMID VECTORS 65
K. J. LEENHOUTS AND G. VENEMA 1. INTRODUCTION 65
IMAGE 3
CONTENTS
2. LACTOCOCCAL VECTOR SYSTEMS 67
GENERAL GENE CLONING VECTORS 67
SPECIAL PURPOSE VECTORS 72
ACKNOWLEDGEMENTS 93
REFERENCES 93
4. VIRULENCE PLASMIDS 95
MARCELO E. TOLMASKY, LUIS A. ACTIS, AND JORGE H. CROSA 1. INTRODUCTION
95
2. ISOLATION OF PLASMIDS 95
LARGE SCALE METHOD 95
SMALL SCALE METHODS 96
3. CURING OF PLASMIDS 99
4. MUTAGENESIS 100
TRANSPOSITION MUTAGENESIS 100
IN VITRO CHEMICAL MUTAGENESIS USING HYDROXYLAMINC 102 SITE-DIRECTED
MUTAGENESIS 103
MARKER EXCHANGE 105
5. FAST AND SMALL ELECTROPHORESIS SEQUENCING GELS 106
6. COMPARISON OF HELICAL STABILITY OF DNA FRAGMENTS 108
GEL PREPARATION AND ELECTROPHORESIS 109
7. IDENTIFICATION OF PLASMID-ENCODED PRODUCTS 110
8. RNA EXTRACTION AND ANALYSIS 113
RNA EXTRACTION 113
NORTHERN BLOT HYBRIDIZATION 114
PRIMER EXTENSION 115
ACKNOWLEDGEMENTS 117
REFERENCES 117
5. PLASMIDS IN AGROBACTERIUM TUME/ACIENS AND RHODOCOCCUS FASCIANS 119
JAN DESOMER, ROLF DEBLAERE, AND MARC VAN MONTAGU 1. INTRODUCTION 119
AGROBACTERIUM TUMEFACIENS 119
RHODOCOCCUS FASCIANS 121
2. IDENTIFICATION OF ENDOGENOUS PLASMIDS BY SMALL SCALE ISOLATION
PROCEDURES 122
AGROBACTERIUM TUMEFACIENS 122
RHODOCOCCUS FASCIANS 126
XI
IMAGE 4
CONTENTS
3. LARGE SCALE PURIFICATION METHODS 131
LARGE SCALE TI PLASMID PREPARATION FROM AGROBACTERIUM 131 RHODOCOCCUS
FASCIANS 133
4. INTRODUCTION OF RECOMBINANT PLASMIDS 135
AGROBACTERIUM TUMEFACIENS 135
IN VITRO TECHNIQUES: HIGH VOLTAGE ELECTROTRANSFORMATION 136 INTRODUCTION
OF RECOMBINANT PLASMIDS IN RHODOCOCCUS FASCIANS BY ELECTROPORATION 138
5. ANALYSIS OF PLASMID-ENCODED FUNCTIONS 139
AGROBACTERIUM TUMEFACIENS TI PLASMIDS 139
RHODOCOCCUS FASCIANS PLASMIDS 141
ANALYSIS OF INSERTION MUTANTS VIA SOUTHERN-TYPE HYBRIDIZATIONS 143
ACKNOWLEDGEMENTS 144
REFERENCES 145
6. STREPTOMYCES PLASMID VECTORS 147
W. WOHLLEBEN AND G. MUTH 1. INTRODUCTION 147
2. AUTONOMOUSLY REPLICATING STREPTOMYCES VECTORS 148
PROPERTIES OF STREPTOMYCES PLASMIDS 148
STREPTOMYCES MARKER GENES 150
STRUCTURE OF REPRESENTATIVE STREPTOMYCES VECTORS 151
3. INTEGRATING VECTORS 155
INTEGRATION SYSTEMS USING SITE-SPECIFIC RECOMBINATION 155 INTEGRATION
SYSTEMS UTILIZING HOMOLOGOUS RECOMBINATION 156
4. GENE DISRUPTION/REPLACEMENT TECHNIQUES USING TEMPERATURE-SENSITIVE
AND NON-REPLICATIVE PLASMIDS 160
GENE DISRUPTION 160
GENE REPLACEMENT 168
GENE RESCUING 170
5. CONCLUSIONS 171
ACKNOWLEDGEMENTS 172
REFERENCES 172
7. USE OF X-PLASMID COMPOSITE VECTORS FOR
EXPRESSION CDNA CLONING 177
TORU MIKI AND STUART A. AARONSON 1. INTRODUCTION 177
XII
IMAGE 5
CONTENTS
2. EXPRESSION CLONING USING UNIDIRECTIONAL CDNA LIBRARIES 177 THE
AUTOMATIC DIRECTIONAL CLONING (ADC) METHOD 177
EUKARYOTIC EXPRESSION SYSTEM 179
PROKARYOTIC EXPRESSION SYSTEM 180
3. GENERATION OF CDNA LIBRARIES BY THE ADC M E T H OD 183
PREPARATION OF LINKER-PRIMERS, ADAPTORS, AND X ARMS 183
CONSTRUCTION OF UNIDIRECTION CDNA LIBRARIES 185
ANALYSIS OF QUALITY OF LIBRARIES 185
AMPLIFICATION OF LIBRARIES 188
4. ANALYSIS OF CLONED CDNAS 188
PLASMID RESCUE 188
RESTRICTION MAPPING 189
INTRODUCTION OF UNIDIRECTIONAL DELETIONS FOR SEQUENCING 191
IDENTIFICATION OF CDNA PRODUCTS 191
USE OF OTHER FEATURES OF THE PCEV27 PLASMID VECTOR 192
5. APPLICATIONS 192
FULL-LENGTH CDNA ISOLATION 192
EUKARYOTIC EXPRESSION CLONING 193
PROKARYOTIC EXPRESSION CLONING 194
6. CONCLUDING REMARKS 195
ACKNOWLEDGEMENTS 195
REFERENCES 195
8. PHAGEMIDS AND OTHER HYBRID VECTORS 197 MICHELLE A. ALTING-MEES, PETER
VAILLANCOURT, AND JAY M. SHORT 1. INTRODUCTION 197
2. EARLY VECTORS 197
FILAMENTOUS PHAGE 197
PLASMIDS AND PBR322 199
EMBL PHAGEMID VECTORS 200
FILAMENTOUS PHAGE ORIGINS 200
SINGLE-STRANDED RESCUE OF PHAGEMID DNA 200
3. NEW APPLICATIONS 202
GENERAL MULTIFUNCTIONAL PHAGEMID CLONING VECTORS 202
EXPRESSION SYSTEMS 202
PHAGE DISPLAY 207
POLYCOS VECTORS 209
PCR CLONING WITH PHAGEMID VECTORS 211
4. PHAGEMIDS AND LAMBDA ZAP CLONING 212
DESCRIPTION AND RELEVANCE OF THE EXCISION PROCESS 213
EUKARYOTIC EXPRESSION/TRANSFECTION USING EXCISABLE LAMBDA/PHAGEMID
HYBRIDS 213
SINGLE CLONE EXCISION 215
XIII
IMAGE 6
CONTENTS
MASS EXCISION 218
NEW APPLICATIONS 219
5. FUTURE 220
REFERENCES 221
9. EXTRACHROMOSOMAL CLONING VECTORS OF
SACCHAROMYCES CEREVISIAE 225
ROBERT S. SIKORSKI 1. INTRODUCTION 225
2. BASIC CLONING VECTORS OF S. CEREVISIAE 225
FUNCTIONAL ELEMENTS 225
BASIC TYPES OF YEAST EXTRACHROMOSOMAL CLONING VECTORS 228
3. MULTIFUNCTIONAL CLONING VECTORS OF S. CEREVISIAE 230
CUSTOMIZING ELEMENTS 230
SELECTED CLONING VECTORS OF S. CEREVISIAE 233
4. YEAST HOST STRAIN 235
5. INTRODUCTION OF DNA INTO S. CEREVISIAE 235
ACKNOWLEDGEMENTS 240
REFERENCES 240
APPENDIX. ADDRESSES OF SUPPLIERS 243
INDEX 245
XIV
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| illustrated | Illustrated |
| indexdate | 2024-07-09T17:27:05Z |
| institution | BVB |
| isbn | 0199634459 0199634440 |
| language | English |
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| physical | XVIII, 252 S. Ill., graph. Darst. |
| publishDate | 1993 |
| publishDateSearch | 1993 |
| publishDateSort | 1993 |
| publisher | IRL Press |
| record_format | marc |
| series | The practical approach series |
| series2 | The practical approach series |
| spelling | Plasmids a practical approach ed. by K. G. Hardy 2. ed. Oxford [u.a.] IRL Press 1993 XVIII, 252 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier The practical approach series 138 Literaturangaben Plazmidi Plasmids Molekulargenetik (DE-588)4039987-4 gnd rswk-swf Plasmid (DE-588)4046270-5 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Plasmid (DE-588)4046270-5 s Methode (DE-588)4038971-6 s DE-604 Molekulargenetik (DE-588)4039987-4 s Hardy, Kimber G. edt The practical approach series 138 (DE-604)BV007921321 138 SWB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=005896230&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Plasmids a practical approach The practical approach series Plazmidi Plasmids Molekulargenetik (DE-588)4039987-4 gnd Plasmid (DE-588)4046270-5 gnd Methode (DE-588)4038971-6 gnd |
| subject_GND | (DE-588)4039987-4 (DE-588)4046270-5 (DE-588)4038971-6 |
| title | Plasmids a practical approach |
| title_auth | Plasmids a practical approach |
| title_exact_search | Plasmids a practical approach |
| title_full | Plasmids a practical approach ed. by K. G. Hardy |
| title_fullStr | Plasmids a practical approach ed. by K. G. Hardy |
| title_full_unstemmed | Plasmids a practical approach ed. by K. G. Hardy |
| title_short | Plasmids |
| title_sort | plasmids a practical approach |
| title_sub | a practical approach |
| topic | Plazmidi Plasmids Molekulargenetik (DE-588)4039987-4 gnd Plasmid (DE-588)4046270-5 gnd Methode (DE-588)4038971-6 gnd |
| topic_facet | Plazmidi Plasmids Molekulargenetik Plasmid Methode |
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