Genetics and molecular biology:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Baltimore u.a.
Johns Hopkins Univ. Press
1993
|
| Ausgabe: | 2. ed. |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVI, 698 S. Ill., graph. Darst. |
| ISBN: | 0801846730 |
Internformat
MARC
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| 245 | 1 | 0 | |a Genetics and molecular biology |c Robert Schleif |
| 250 | |a 2. ed. | ||
| 264 | 1 | |a Baltimore u.a. |b Johns Hopkins Univ. Press |c 1993 | |
| 300 | |a XVI, 698 S. |b Ill., graph. Darst. | ||
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| 650 | 7 | |a Moleculaire biologie |2 gtt | |
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Datensatz im Suchindex
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| adam_text | Genetics and
Molecular Biology
SECONDEDITION
Robert Schleif
Department of Biology
The Johns Hopkins University
Baltimore, Maryland
The Johns Hopkins University Press Baltimore and London
Contents
1 An Overview of Cell Structure and Function 1
Cell s Need for Immense Amounts of Information 2
Rudiments of Prokaryotic Cell Structure 2
Rudiments of Eukaryotic Cell Structure 6
Packing DNA into Cells 7
Moving Molecules into or out of Cells 8
Diffusion within the Small Volume of a Cell 13
Exponentially Growing Populations 14
Composition Change in Growing Cells 15
Age Distribution in Populations of Growing Cells 15
Problems 16
-References 18
2 Nucleic Acid and Chromosome Structure 21
The Regular Backbone of DNA 22
Grooves in DNA and Helical Forms of DNA 23
Dissociation and Reassociation of Base-paired Strands 26
Reading Sequence without Dissociating Strands 27
Electrophoretic Fragment Separation 28
Bent DNA Sequences 29
Measurement of Helical Pitch 31
Topological Considerations in DNA Structure 32
Generating DNA with Superhelical Turns 33
Measuring Superhelical Turns 34
Determining Lk, Tw, and Wr in Hypothetical Structures 36
Altering Linking Number 37
Biological Significance of Superhelical Turns 39
The Linking Number Paradox of Nucleosomes 40
General Chromosome Structure 41
Southern Transfers to Locate Nucleosomes on Genes 41
ARS Elements, Centromeres, and Telomeres 43
Problems 44
References 47
3 DNA Synthesis 53
A Enzymology 54
Proofreading, Okazaki Fragments, and DNA Ligase 54
Detection and Basic Properties of DNA Polymerases 57
In vitro DNA Replication 60
Error and Damage Correction 62
B Physiological Aspects 66
DNA Replication Areas in Chromosomes 66
Bidirectional Replication from E coli Origins 67
The DNA Elongation Rate 69
Constancy of the E coli DNA Elongation Rate 71
Regulating Initiations 72
Gel Electrophoresis Assay of Eukaryotic Replication Origins 74
How Fast Could DNA Be Replicated? 76
Problems 78
References 79
4 RNA Polymerase and RNA Initiation 85
Measuring the Activity of RNA Polymerase 86
Concentration of Free RNA Polymerase in Cells 89
The RNA Polymerase in Escherichia coli 90
Three RNA Polymerases in Eukaryotic Cells 91
Multiple but Related Subunits in Polymerases 92
Multiple Sigma Subunits 95
The Structure of Promoters 96
Enhancers 99
Enhancer-Binding Proteins 100
DNA Looping in Regulating Promoter Activities 102
Steps of the Initiation Process 104
Measurement of Binding and Initiation Rates 105
Relating Abortive Initiations to Binding and Initiating 107
Roles of Auxiliary Transcription Factors 109
Melted DNA Under RNA Polymerase 110
Problems 111
References 113
5 Transcription, Termination, and RNA Processing 119
Polymerase Elongation Rate 119
Contents ix
Transcription Termination at Specific Sites 121
Termination 122
Processing Prokaryotic RNAs After Synthesis • 125
SI Mapping to Locate 5 and 3 Ends of Transcripts 126
Caps, Splices, Edits, and Poly-A Tails on Eukaryotic RNAs 127
The Discovery and Assay of RNA Splicing 128
Involvement of the U1 snRNP Particle in Splicing 131
Splicing Reactions and Complexes 134
The Discovery of Self-Splicing RNAs 135
A Common Mechanism for Splicing Reactions 137
Other RNA Processing Reactions 139
Problems 140
References 142
6 Protein Structure 149
The Amino Acids 150
The Peptide Bond 153
Electrostatic Forces that Determine Protein Structure 154
Hydrogen Bonds and the Chelate Effect 158
Hydrophobic Forces 159
Thermodynamic Considerations of Protein Structure 161
Structures within Proteins 162
The Alpha Helix, Beta Sheet, and Beta Turn 164
Calculation of Protein Tertiary Structure 166
Secondary Structure Predictions 168
Structures of DNA-Binding Proteins 170
Salt Effects on Protein-DNA Interactions 173
Locating Specific Residue-Base Interactions 174
Problems 175
References 177
7 Protein Synthesis 183
A Chemical Aspects 184
Activation of Amino Acids During Protein Synthesis 184
Fidelity of Aminoacylation 185
How Synthetases Identify the Correct tRNA Molecule 187
Decoding the Message 188
Base Pairing between Ribosomal RNA and Messenger 191
Experimental Support for the Shine-Dalgarno Hypothesis 192
Eukaryotic Translation and the First AUG 194
Tricking the Translation Machinery into Initiating 195
Protein Elongation 197
Peptide Bond Formation 198
Translocation 198
Termination, Nonsense, and Suppression 199
Chaperones and Catalyzed Protein Folding 202
Resolution of a Paradox 202
B Physiological Aspects 203
Messenger Instability 203
Protein Elongation Rates 204
Directing Proteins to Specific Cellular Sites 207
Verifying the Signal Peptide Model 208
The Signal Recognition Particle and Translocation 210
Expectations for Ribosome Regulation 211
Proportionality of Ribosome Levels and Growth Rates 212
Regulation of Ribosome Synthesis 214
Balancing Synthesis of Ribosomal Components 216
Problems 218
References 220
8 Genetics 227
Mutations 227
Point Mutations, Deletions, Insertions, and Damage 228
Classical Genetics of Chromosomes 231
Complementation, Cis, Trans, Dominant, and Recessive 233
Mechanism of a trans Dominant Negative Mutation 234
Genetic Recombination 235
Mapping by Recombination Frequencies 236
Mapping by Deletions 239
Heteroduplexes and Genetic Recombination 239
Branch Migration and Isomerization 241
Elements of Recombination in E coli, RecA, RecBCD, and Chi 243
Genetic Systems 244
Growing Cells for Genetics Experiments 245
Testing Purified Cultures, Scoring 246
Isolating Auxotrophs, Use of Mutagens and Replica Plating 247
Genetic Selections 248
Mapping with Generalized Transducing Phage 250
Principles of Bacterial Sex 251
Elements of Yeast Genetics 253
Elements of Drosophila Genetics 254
Isolating Mutations in Muscle or Nerve in Drosophila 255
Fate Mapping and Study of Tissue-Specific Gene Expression 256
Problems 257
References 261
9 Genetic Engineering and Recombinant DNA 265
The Isolation of DNA 266
The Biology of Restriction Enzymes 268
Cutting DNA with Restriction Enzymes 271
Isolation of DNA Fragments 272
Contents xi
Joining DNA Fragments 272
Vectors: Selection and Autonomous DNA Replication 274
Plasmid Vectors 274
A Phage Vector for Bacteria 278
Vectors for Higher Cells 279
Putting DNA Back into Cells 281
Cloning from RNA 282
Plaque and Colony Hybridization for Clone Identification 283
Walking Along a Chromosome to Clone a Gene 284
Arrest of Translation to Assay for DNA of a Gene 285
Chemical DNA Sequencing 286
Enzymatic DNA Sequencing 289
Problems 291
References 293
10 Advanced Genetic Engineering 297
Finding Clones from a Known Amino Acid Sequence 297
Finding Clones Using Antibodies Against a Protein 298
Southern, Northern, and Western Transfers 300
Polymerase Chain Reaction 302
Isolation of Rare Sequences Utilizing PCR 305
Physical and Genetic Maps of Chromosomes 306
Chromosome Mapping 307
DNA Fingerprinting—Forensics 310
Megabase Sequencing 311
Footprinting, Premodification and Missing Contact Probing 313
Antisense RNA: Selective Gene Inactivation 317
Hypersynthesis of Proteins 317
Altering Cloned DNA by in vitro Mutagenesis 318
Mutagenesis with Chemically Synthesized DNA 321
Problems 323
References 325
11 Repression and the lac Operon 331
Background of the lac Operon 332
The Role of Inducer Analogs in the Study of the lac Operon 334
Proving lac Repressor is a Protein 335
An Assay for lac Repressor 336
The Difficulty of Detecting Wild-Type lac Repressor 338
Detection and Purification of lac Repressor 340
Repressor Binds to DNA: The Operator is DNA 341
The Migration Retardation Assay and DNA Looping 343
The Isolation and Structure of Operator 344
In vivo Affinity of Repressor for Operator 346
The DNA-binding Domain of lac Repressor 346
A Mechanism for Induction 348
Problems 349
References 353
12 Induction, Repression, and the araBAD Operon 359
The Sugar Arabinose and Arabinose Metabolism 360
Genetics of the Arabinose System 362
Detection and Isolation of AraC Protein 364
Repression by AraC 366
Regulating AraC Synthesis 368
Binding Sites of the ara Regulatory Proteins 369
DNA Looping and Repression of araBAD 371
In vivo Footprinting Demonstration of Looping 373
How AraC Protein Loops and Unloops 373
Why Looping is Biologically Sensible 376
Why Positive Regulators are a Good Idea 376
Problems 377
References 379
13 Attenuation and the trp Operon 385
The Aromatic Amino Acid Synthetic Pathway and its Regulation 386
Rapid Induction Capabilities of the trp Operon 388
The Serendipitous Discovery of trp Enzyme Hypersynthesis 390
Early Explorations of the Hypersynthesis 392
trp Multiple Secondary Structures in trp Leader RNA 396
Coupling Translation to Termination 397
RNA Secondary Structure and the Attenuation Mechanism 399
Other Attenuated Systems: Operons, Bacillus subtilis and HIV 400
Problems 402
References 404
14 Lambda Phage Genes and Regulatory Circuitry 409
A The Structure and Biology of Lambda 410
The Physical Structure of Lambda 410
The Genetic Structure of Lambda 411
Lysogeny and Immunity 413
Lambda s Relatives and Lambda Hybrids 414
B Chronology of a Lytic Infective Cycle 415
Lambda Adsorption to Cells 415
Early Transcription of Genes N and Cro 416
N Protein and Antitermination of Early Gene Transcription 417
The Role of Cro Protein 418
Initiating DNA Synthesis with the O and P Proteins 418
Proteins Kil, 7, p, and Exo 419
Q Protein and Late Proten Synthesis 420
Lysis 421
Contents xiii
C The Lysogenic Infective Cycle and Induction of Lyso-
gens 422
Chronology of Becoming a Lysogen 422
Site for Cro Repression and CI Activation 423
Cooperativity in Repressor Binding and its Measurement 426
The Need for and the Realization of Hair-Trigger Induction 427
Induction from the Lysogenic State 429
Entropy, a Basis for Lambda Repressor Inactivation 431
Problems 433
References 435
15 Xenopus 5S RNA Synthesis 443
Biology of 5S RNA Synthesis in Xenopus 443
In vitro 5S RNA Synthesis 446
TFIIIA Binding ,to the Middle of its Gene as Well as to RNA 447
Switching from Oocyte to Somatic 5S Synthesis 450
Structure and Function of TFIIIA 452
Problems 453
References 454
16 Regulation of Mating Type in Yeast 457
The Yeast Cell Cycle 458
Mating Type Conversion in Saccharomyces cerevisiae 459
Cloning the Mating Type Loci in Yeast 460
Transfer of Mating Type Gene Copies to an Expression Site 461
Structure of the Mating Type Loci 462
The Expression and Recombination Paradoxes 463
Silencing HML and HMR 464
Isolation of a2 Protein 466
a2 and MCM1 468
—Sterile Mutants, Membrane Receptors and G Factors 469
DNA Cleavage at the MAT Locus 471
DNA Strand Inheritance and Switching in Fission Yeast 472
Problems 474
References 475
17 Genes Regulating Development 479
General Considerations on Signaling 479
Outline of Early Drosophila Development 482
Classical Embryology 484
Using Genetics to Begin Study of Developmental Systems 484
Cloning Developmental Genes 487
Enhancer Traps for Detecting and Cloning Developmental Genes 487
Expression Patterns of Developmental Genes 488
Similarities Among Developmental Genes 491
Overall Model of Drosophila Early Development 491
Problems 492
References 492
18 Lambda Phage Integration and Excision 497
Mapping Integrated Lambda 498
Simultaneous Deletion of Chromosomal and Lambda DNA 499
DNA Heteroduplexes Prove that Lambda Integrates 501
Gene Order Permutation and the Campbell Model 501
Isolation of Integration-Defective Mutants 503
Isolation of Excision-Deficient Mutants 504
Properties of the int and xis Gene Products 506
Incorrect Excision and gal and bio Transducing Phage 506
Transducing Phage Carrying Genes Other than gal and bio 508
Use of Transducing Phage to Study Integration and Excision 509
The Double att Phage, att2 510
Demonstrating Xis is Unstable 512
Inhibition By a Downstream Element 513
In vitro Assay of Integration and Excision 515
Host Proteins Involved in Integration and Excision 517
Structure of the att Regions 517
Structure of the Intasome 519
Holliday Structures and Branch Migration in Integration 521
Problems 523
References 525
19 Transposable Genetic Elements 531
IS Elements in Bacteria 532
Structure and Properties of IS Elements 534
Discovery of Tn Elements 536
Structure and Properties of Tn Elements 538
Inverting DNA Segments by Recombination, Flagellin Synthesis 540
Mu Phage As a Giant Transposable Element 542
An Invertible Segment of Mu Phage 544
In vitro Transposition, Threading or Global Topology? 545
Hopping by TnlO 547
Retrotransposons in Higher Cells 550
An RNA Transposition Intermediate 552
P Elements and Transformation 553
P Element Hopping by Chromosome Rescue 555
Problems 557
References 558
20 Generating Genetic Diversity: Antibodies 563
The Basic Adaptive Immune Response 563
Telling the Difference Between Foreign and Self 565
The Number of Different Antibodies Produced 566
Contents xv
Myelomas and Monoclonal Antibodies 567
The Structure of Antibodies 569
Many Copies of V Genes and Only a Few C Genes 571
The J Regions 573
The D Regions in H Chains 575
Induced Mutations and Antibody Diversity 577
Class Switching of Heavy Chains 577
Enhancers and Expression of Immunoglobulin Genes 578
The AIDS Virus 579
Engineering Antibody Synthesis in Bacteria 580
Assaying for Sequence Requirements of Gene Rearrangements 582
Cloning the Recombinase 584
Problems 584
References 586
21 Biological Assembly, Ribosomes and Lambda Phage 591
A Ribosome Assembly 592
RNAse and Ribosomes 592
The Global Structure of Ribosomes 593
Assembly of Ribosomes 595
Experiments with in vitro Ribosome Assembly 597
Determining Details of Local Ribosomal Structure 599
B Lambda Phage Assembly 601
General Aspects 601
The Geometry of Capsids 602
The Structure of the Lambda Particle 605
The Head Assembly Sequence and Host Proteins 606
Packaging the DNA and Formation of the cos Ends 607
Formation of the Tail 609
In vitro Packaging 610
Problems 610
References 613
22 Chemotaxis 619
Assaying Chemotaxis 620
Fundamental Properties of Chemotaxis 622
Genetics of Motility and Chemotaxis 624
How Cells Swim 625
The Mechanism of Chemotaxis 627
The Energy for Chemotaxis 629
Adaptation 630
Methylation and Adaptation 632
Phosphorylation and the Rapid Response 633
Problems 635
References 637
23 Oncogenesis, Molecular Aspects 643
Bacterially Induced Tumors in Plants 644
Transformation and Oncogenesis by Damaging the Chromosome 645
Identifying a Nucleotide Change Causing Cancer 647
Retroviruses and Cancer 650
Cellular Counterparts of Retroviral Oncogenes 653
Identification of the src and sis Gene Products 654
DNA Tumor Viruses 656
Recessive Oncogenic Mutations, Tumor Suppressors 658
The ras-fos-jun Pathway 660
Directions for Future Research in Molecular Biology 661
Problems 661
References 663
Hints and Solutions to Odd-Numbered Problems 667
Index 685
|
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| illustrated | Illustrated |
| indexdate | 2024-07-09T17:18:47Z |
| institution | BVB |
| isbn | 0801846730 |
| language | English |
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| physical | XVI, 698 S. Ill., graph. Darst. |
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| spelling | Schleif, Robert F. Verfasser aut Genetics and molecular biology Robert Schleif 2. ed. Baltimore u.a. Johns Hopkins Univ. Press 1993 XVI, 698 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Genetica molecular e de microorganismos larpcal Genetica gtt Moleculaire biologie gtt Molecular Biology Molecular genetics Molekulargenetik (DE-588)4039987-4 gnd rswk-swf Genetik (DE-588)4071711-2 gnd rswk-swf Genetik (DE-588)4071711-2 s DE-604 Molekulargenetik (DE-588)4039987-4 s HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=005517937&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Schleif, Robert F. Genetics and molecular biology Genetica molecular e de microorganismos larpcal Genetica gtt Moleculaire biologie gtt Molecular Biology Molecular genetics Molekulargenetik (DE-588)4039987-4 gnd Genetik (DE-588)4071711-2 gnd |
| subject_GND | (DE-588)4039987-4 (DE-588)4071711-2 |
| title | Genetics and molecular biology |
| title_auth | Genetics and molecular biology |
| title_exact_search | Genetics and molecular biology |
| title_full | Genetics and molecular biology Robert Schleif |
| title_fullStr | Genetics and molecular biology Robert Schleif |
| title_full_unstemmed | Genetics and molecular biology Robert Schleif |
| title_short | Genetics and molecular biology |
| title_sort | genetics and molecular biology |
| topic | Genetica molecular e de microorganismos larpcal Genetica gtt Moleculaire biologie gtt Molecular Biology Molecular genetics Molekulargenetik (DE-588)4039987-4 gnd Genetik (DE-588)4071711-2 gnd |
| topic_facet | Genetica molecular e de microorganismos Genetica Moleculaire biologie Molecular Biology Molecular genetics Molekulargenetik Genetik |
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