Stains and cytochemical methods:
Saved in:
| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
New York u.a.
Plenum Press
1993
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XVII, 455 S. Ill. |
| ISBN: | 0306442949 |
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MARC
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Record in the Search Index
| _version_ | 1804122609795727360 |
|---|---|
| adam_text | Stains and
Cytochemical Methods
M A Hayat
Department of Biology
Kean College
Union, New jersey
Plenum Press • New York and London
Contents
Chapter 1 Introduction 1
Chapter 2 Resolution and Image Contrast 7
Resolution 7
Image Contrast 7
Factors Affecting Contrast 9
Chapter 3 Staining Parameters 13
Stain Penetration 13
Rate of Staining 16
Duration of Staining 18
Size of Stain Aggregates : 19
Stain Specificity 20
Chapter 4 Effects of Specimen Processing on Staining 23
Effects of Fixation 24
Effects of Buffers 29
Effects of Dehydration and Embedding 29
ix
x Contents
Chapter 5 Staining Artifacts 33
Precautions for Minimizing Artifactual Staining
Precipitates 34
Removal of Artifactual Staining Precipitates 37
Chapter 6 Staining Procedures 41
Double Staining with Uranyl Acetate and Lead Citrate 41
Multiple Grid Staining 43
Multiple Staining 45
Selective Heavy Metal Staining for High-Resolution
Electron Microscopy 46
Staining for High-Voltage Electron Microscopy 47
Staining Procedures 49
Staining of Thin Cryosections 52
Staining of Thin Lowicryl K4M Sections 53
Procedure 54
Chapter 7 Staining of Semithin Resin Sections 55
Stain Penetration 56
Hemo-De 56
Dimethyl Formamide 56
Elevated Temperatures 57
Potassium Permanganate-Oxalic Acid 57
Animal Tissues (General Method 1) 57
Basic Fuchsin-Toluidine Blue 57
Animal Tissues (General Method 2) 58
Basic Fuchsin-Methylene Blue 58
Cartilage/Bone 60
Azure II-Methylene Blue-Safranin O 60
Elastic Fibers 60
Miller s Stain-Gill s III Hematoxylin-Fast Green 60
Glycogen 62
Periodic Acid-Schiff Reaction 62
Lipids (Animal Tissues) 63
Hematoxylin-Eosin Y 63
Lipids (Plant Tissues) 64
Periodic Acid-Schiff-Toluidine Blue O 64
Contents xi
Muscle 65
/ -Phenylenediamine 65
Nervous Tissue 66
Hematoxylin-Eosin Y 66
Nucleic Acids 66
Azure B Bromide Stain for DNA and RNA 66
Periodic Acid-Schiff-Positive Materials 67
Periodic Acid-Schiff Reagent-Methylene Blue-Azure II 67
Plant Tissues 68
Thionin-Acridine Orange 68
Proteins (Plant Tissues) 69
Ponceau 2R-Periodic Acid 69
Chapter 8 Staining and Related Reagents 71
Acridine Orange 72
Purity of the Dye 73
Reaction with Complex Carbohydrates 74
Reaction with Nuclear Components 75
Specific Reaction with Nucleic Acids 77
Effect on the Denaturation of Nucleic Acids 78
Identification of Transformed Cells 79
Nonyl Acridine Orange 79
Alcian Blue 80
Purification of Alcian Blue 81
Chemical Composition 81
Mechanism of Staining 82
Reaction with Nucleic Acids 84
Critical Electrolyte Concentration 84
Role of pH 86
Rate of Staining 87
Specificity of Staining for Sulfated Glycosaminoglycans 88
Avidin-Biotin 89
Avidin-Ferritin 92
Avidin-Colloidal Gold 92
Stabilization of Gold Sols with Avidin 93
Avidin-Biotin-Peroxidase Complex 93
Preembedding Procedure 94
Postembedding Procedure 94
Benzidine Dihydrochloride 95
Bismuth 97
Mechanism of Staining 99
Effects of Aldehydes on Bismuth Staining 100
xii Contents
General Staining Procedure 101
Selective Staining of Nucleoproteins 101
Staining of Synapses 101
Cerium 102
Light Microscopy 103
Cetylpyridinium 105
Colloidal Gold 106
Determination of Gold Particle Size 106
Methods for Preparing Colloidal Gold Particles of Various
Diameters 107
Reduction by White Phosphorus 107
Reduction by Sodium Citrate and Tannic Acid 108
Reduction by Sodium Citrate 109
Procedure for Preparation of Protein A-Gold Complex I l l
Labeling 112
Immunoglobulin-Colloidal Gold Method 112
Lectin-Colloidal Gold Method 113
Enzyme-Colloidal Gold Method 115
Preparation of Enzyme-Gold Complex 115
Labeling 116
Preparation and Immunogold Labeling of Thin
Cryosections 116
Multiple Immunogold Labeling 119
Immunogold-Silver Staining 123
Technical Considerations 124
Paraffin Sections 125
Semithin Resin Sections 126
Thin Resin Sections 127
Thin Cryosections 127
Cuprolinic Blue - 128
Reaction with Nucleic Acids 132
General Procedure 132
Diaminobenzidine-Osmium Tetroxide 133
Ferritin 133
Cationic Ferritin 134
Mechanism of Binding 134
Mode of Cationic Ferritin Application 136
Filipin 140
Mechanism of Reaction 144
Fixation 146
Limitations 147
Fluoro-Gold 148
Horseradish Peroxidase-Based Methods 149
Nonspecific Staining 151
Chromogens 152
Contents xiii
Stabilizer 153
Diaminobenzidine 153
Silver Intensification of Nickel-Diaminobenzidine
Staining 156
Single-Step Silver Enhancement of Diaminobenzidine
Staining 156
Gold Intensification of Diaminobenzidine Staining 157
Ferric Ferricyanide Intensification of Diaminobenzidine
Staining 158
Platinum Staining of Peroxidatic Reaction Product 158
Cobalt-Glucose Oxidase Intensification of Diaminobenzi-
dine Staining 159
Imidazole-Diaminobenzidine 159
Silver-Intensified Gold-Horseradish Peroxidase Method 160
Tetramethylbenzidine 160
pH 162
Limitations 163
Procedure 1 164
Procedure 2 165
Tetramethylbenzidine-Tungstate Method 166
Imidazole-Osmium Tetroxide Complexes 168
Iodide-Osmium Tetroxide Mixtures 173
Mechanism of Staining 176
Factors Affecting the ZIO Reaction 178
pH 178
Buffers 179
Size of the Tissue Block 179
Prefixation 180
Duration and Temperature of Staining 180
Staining Specificity 181
Zinc Iodide-Osmium Tetroxide 182
Staining of Langerhans Cells 183
Staining of Synaptic Vesicles 183
Iron : 184
Mechanism of Staining 185
Colloidal Iron 187
Iron Diamine 192
pH 194
Rate of Penetration 196
Mode of Staining 196
Staining Solutions 197
Colloidal Ammonium Ferric Glycerate (Dialyzed
Iron) 198
Positive Ferric Oxide Solution 198
Negative Ferric Oxide Solutions 199
xiv Contents
Negative Colloidal Ferric Hydroxide 199
High-Iron Diamine 199
Low-Iron Diamine 200
Colloidal Iron in Cacodylate Buffer 200
Colloidal Iron in Ammonium Cacodylate Solution 200
Ferric Hydroxide Colloid 201
Labeling with Colloidal Ferric Particles and Magnetic
Sorting 201
Lanthanum 202
Mechanism of Staining 204
Penetration 205
Reaction with Proteins 207
Reaction with Nucleic Acids 208
Reaction with Membrane Components 208
Lanthanum as a Tracer for Extracellular Space 209
Lanthanum as a Tracer for Intercellular Junctions 209
Lanthanum as an Intracellular Marker 210
Lanthanum as a Probe for Calcium-Binding Sites 210
General Methodology 212
Fixation and Staining Procedures 213
Lead 214
Mechanism of Staining 215
Reaction with Membranes 218
Reaction with Glycogen 218
Reaction with Other Cell Components 220
Lead Acetate 221
Lead Aspartate 223
Lead Citrate 223
Lead Hydroxide 225
Lead Tartrate 227
En Bloc Staining with Lead 228
Double Lead Staining Method 229
Role of Lead Citrate in Uranyl Acetate Staining 229
Lectins 230
Concanavalin A 236
Wheat Germ Agglutinin 238
Castor Bean Lectin 239
Soybean Agglutinin 240
Peanut Lectin 240
Lentil Lectin 241
Derivatized Lectins 241
Lectin-Horseradish Peroxidase Methods 241
Concanavalin A-Horseradish Peroxidase 242
Lectin Conjugates 244
Concanavalin A-Ferritin 246
Contents xv
Peanut Lectin-Horseradish Peroxidase 247
Wheat Germ Agglutinin-Ferritin 247
Lucifer Yellow CH 248
Intracellular Injection Procedure 249
In Vitro Staining Procedure 250
Malachite Green 251
Mechanism of Staining 252
Osmium Ammine 254
Synthesis of Osmium Ammine 259
Osmium Tetroxide 260
Osmium Impregnation and Other Methods 263
Osmium Tetroxide-Potassium Ferricyanide or
Ferrocyanide 266
Fixation and Staining Procedures 271
Selective Staining of Intercellular Space in Epithelial
Tissues 271
Oxalate-Glutaraldehyde 272
/ -Phenylenediamine 273
Phosphotungstic Acid 274
Mechanism of Staining 279
pH 280
Specificity of Staining 281
Ethanolic Phosphotungstic Acid 284
Polyethyleneimine 287
Procedure 1: Intravenous Injection 289
Procedure 2: Vascular Perfusion 289
Procedure 3: Immersion 290
Procedure 4: Freeze-Substitution 290
PolymyxinB 291
Potassium Pyroantimonate 294
Mechanism of Staining 295
Effects of Fixation 296
Specificity ofReaction 297
Reproducibility of Results 299
Limitations of Method 300
Fixation and Staining Procedures 301
Ruthenium Hexammine Trichloride 302
Fixation and Staining Procedure 304
Ruthenium Red 305
Purification of Commercial Ruthenium Red 307
Reaction with Nucleic Acids 307
Reaction with Pectin 308
Mechanism of Staining 308
xvi Contents
Role of Osmium Tetroxide in Staining with Ruthenium
Red 310
Effect on Cell Physiology 311
Penetration 314
Fixation and Staining Procedures 315
Silver 318
Reaction with Carbohydrates 319
Reaction with Proteins 320
Reaction with Nucleic Acids 321
Reaction with Catecholamines and Related Compounds 323
Role of Fixation in Silver Staining 325
Fixation and Staining Procedures 326
Ammoniacal Silver 326
Silver Methenamine 327
Silver Nitrate 332
Silver Proteinate 334
Silver Lactate-Osmium Tetroxide 336
Tannic Acid 336
Reaction with Proteins 339
Reaction with Carbohydrates 341
Reaction with Lipids 341
Reaction with Membranes 342
Role in the Study of Exocytosis 342
Fixation and Mordanting Effects of Tannic Acid 343
Penetration 344
Negative Staining with Tannic Acid 344
Fixation and Staining Procedures 346
General Procedures 346
Visualization of Cholinergic Synaptic Junctions 346
Visualization of Elastin 346
Visualization of Exocytotic Products 347
Thiosemicarbazide and Thiocarbohydrazide 347
Periodic Acid-Thiosemicarbazide or Thiocarbohydrazide-
Silver Proteinate 348
Periodic Acid-Thiosemicarbazide or Thiocarbohydrazide-
Osmium Tetroxide 349
Sodium Periodate-Thiosemicarbazide-Osmium Tetroxide 350
Ninhydrin-Thiocarbohydrazide-Silver Proteinate 351
Tungstate 352
Uranyl Preparations 353
Uranyl Acetate 354
Uranyl Nitrate 355
Mechanism of Staining 356
Reaction with Nucleic Acids 357
Contents xvii
Reaction with Proteins 359
Reaction with Lipids and Membranes 360
Uranaffin Reaction 362
Uranaffin Method 364
Localization of Phosphonucleotides 364
Factors Affecting Uranyl Staining 364
pH , 364
Buffer Types 366
Fixation and Embedding Methods 367
Overall Effect on Tissues 367
Staining Solutions 369
Chapter 9 Subcellular Localization of Metals 373
Autometallography 374
The Developer 374
Beryllium 375
Cadmium 375
Gold 377
Iron 377
Iron Nitrilotriacetate-Acid Ferrocyanide-Silver Protein-
ate Method 377
Procedure 377
Lead 378
Mercury 379
Silver 381
Tungsten 382
Zinc 383
References 385
Subject Index 443
|
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| illustrated | Illustrated |
| indexdate | 2024-07-09T17:16:36Z |
| institution | BVB |
| isbn | 0306442949 |
| language | English |
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| physical | XVII, 455 S. Ill. |
| publishDate | 1993 |
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| publisher | Plenum Press |
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| spelling | Hayat, M. A. 1936- Verfasser (DE-588)129731447 aut Stains and cytochemical methods M. A. Hayat New York u.a. Plenum Press 1993 XVII, 455 S. Ill. txt rdacontent n rdamedia nc rdacarrier Coloration (Microscopie) Microscopie électronique - Technique Electron microscopy Technique Microscopy, Electron methods Staining and Labeling methods Stains and staining (Microscopy) Elektronenmikroskopie (DE-588)4014327-2 gnd rswk-swf Färben (DE-588)4016254-0 gnd rswk-swf Cytochemie (DE-588)4070172-4 gnd rswk-swf Biologie (DE-588)4006851-1 gnd rswk-swf Elektronenmikroskopie (DE-588)4014327-2 s Färben (DE-588)4016254-0 s DE-604 Cytochemie (DE-588)4070172-4 s Biologie (DE-588)4006851-1 s HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=005427599&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Hayat, M. A. 1936- Stains and cytochemical methods Coloration (Microscopie) Microscopie électronique - Technique Electron microscopy Technique Microscopy, Electron methods Staining and Labeling methods Stains and staining (Microscopy) Elektronenmikroskopie (DE-588)4014327-2 gnd Färben (DE-588)4016254-0 gnd Cytochemie (DE-588)4070172-4 gnd Biologie (DE-588)4006851-1 gnd |
| subject_GND | (DE-588)4014327-2 (DE-588)4016254-0 (DE-588)4070172-4 (DE-588)4006851-1 |
| title | Stains and cytochemical methods |
| title_auth | Stains and cytochemical methods |
| title_exact_search | Stains and cytochemical methods |
| title_full | Stains and cytochemical methods M. A. Hayat |
| title_fullStr | Stains and cytochemical methods M. A. Hayat |
| title_full_unstemmed | Stains and cytochemical methods M. A. Hayat |
| title_short | Stains and cytochemical methods |
| title_sort | stains and cytochemical methods |
| topic | Coloration (Microscopie) Microscopie électronique - Technique Electron microscopy Technique Microscopy, Electron methods Staining and Labeling methods Stains and staining (Microscopy) Elektronenmikroskopie (DE-588)4014327-2 gnd Färben (DE-588)4016254-0 gnd Cytochemie (DE-588)4070172-4 gnd Biologie (DE-588)4006851-1 gnd |
| topic_facet | Coloration (Microscopie) Microscopie électronique - Technique Electron microscopy Technique Microscopy, Electron methods Staining and Labeling methods Stains and staining (Microscopy) Elektronenmikroskopie Färben Cytochemie Biologie |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=005427599&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT hayatma stainsandcytochemicalmethods |