Methods in phytobacteriology:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | Undetermined |
| Veröffentlicht: |
Budapest
Akad. Kiadó
1990
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Literaturangaben |
| Beschreibung: | XIV, 568 S. Ill., graph. Darst. |
| ISBN: | 9630549557 |
Internformat
MARC
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| 245 | 1 | 0 | |a Methods in phytobacteriology |c ed. by Z. Klement ... |
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Datensatz im Suchindex
| _version_ | 1804122050645721088 |
|---|---|
| adam_text | METHODS
IN PHYTOBACTERIOLOGY
edited by
Z KLEMENT
Plant Protection Institute of the Hungarian Academy of Sciences
Budapest, Hungary
K RUDOLPH
Institute for Plant Pathology and Plant Protection, University of Gottingen
Gottingen, Federal Republic of Germany
D C SANDS
Department of Plant Pathology, Montana State University
Bozeman U S A
, 1828-m
AKADEMIAI KIADO, BUDAPEST 1990
CONTENTS*
List of Contributors IX
List of Referees XV
Preface XVII
Part I Identification/Diagnosis 1
Chapter 1 1 General microbiological techniques 3
1 1 Historical introduction 4
2 Equipment of a standard phytobacteriological labo
ratory 4
3 Sterilization of equipment and working place 8
4 Preparation and sterilization of culture media 12
5 Cultivation of bacteria 14
6 Safety precautions 18
1 2 Description and classification of disease symptoms 21
1 3 Microscopical techniques for bacteria 27
3 2 Light microscopy 28
3 Transmission electron microscopy 29
4 Scanning electron microscopy 40
1 4 Isolation of bacteria 43
4 1 Selection and transportation of specimens 45
2 Extraction of bacteria from infected material 47
3 Pure bacterial culture 51
4 Handling different sources of phytopathogenic bacte
ria 55
5 Special requirements for certain bacterial species 57
6 Generally suited media 59
7 Selective media: principles of design and use 62
8 Differentiation of commonly isolated pathogenic
species 65
9 Fastidious prokaryotes 75
10 Bacterial species of minor importance as plant
pathogens 85
11 Reasons for unsuccessful trials in isolating phytopa
thogenic bacteria 85
* The contents of the more important headings arc given here The detailed subchapter headings arc
at the beginning of each individual chapter
VI Contents
1 5 Inoculation of plant tissues 95
5 1 Introduction and general instructions 96
2 Leaf spot and blight diseases 103
3 Canker and dieback diseases 104
4 Wilt diseases 106
5 Soft rot diseases Ill
6 Tumorous diseases 114
7 Difficulties encountered with inoculation experiments 118
1 6 Taxonomy 125
1 7 Physiological criteria-determinative tests 133
1 8 Numerical analysis of phenotypic features 145
1 9 Serology 153
2 Selection and preparation of antigens (immunogens) 155
3 Immunization 157
4 Preparation of antisera 159
5 Preparation of gammaglobulin (IgG) 159
6 Serological tests 160
7 Immunoelectrophoresis 165
8 Immuno-isolation technique 169
9 Immunofluorescence assays 172
10 Enzyme-linked immunosorbent assay (ELISA) 177
11 Production of monoclonal antibodies 180
12 Immuno gold staining (IGS) and immuno gold silver
staining (IGSS) 185
1 10 Polyacrylamide gel electrophoresis of bacterial proteins 191
1 11 Identification of bacteria through fatty acid analysis 199
1 12 Bacteriophages 205
1 13 Characterization by nucleic acids 215
13 2 DNA extraction and purification 216
3 The determination of DNA base composition 218
4 Comparative study of total genome DNA 218
5 Ribosomal RNA as a taxonomic tool 220
6 DNA probes 224
7 Restriction endonuclease cleavage patterns 229
1 14 Pigments 233
1 15 Bacteriocins V 245
1 16 Toxins as taxonomic features 251
1 17 Proposals for a quick practical identification 269
1 18 Preservation oif bacteria 275
Part II Control 279
Chapter II 1 Epidemiology 281
1 1 Survival of pathogens 281
2 Forecasting models 288
11 2 Estimating losses caused by bacteria 297
11 3 Methods in agronomy to reduce bacterial diseases 301
11 4 Methods for phytosanitary measures 307
11 5 Examples of eradication methods 315
11 6 Bactericides 319
11 7 Antibiotics 327
11 8 Breeding for resistance 333
8 1 Screening for resistance 334
2 Determination of bacterial races 354
Contents VII
3 Resistance screening with mixtures of strains and
races with individual bacterial isolates 356
4 Bacterial growth assessment in the plant to determine
degree of resistance 356
II 9 Biological control 369
Part III Biochemistry and host-parasite relationships 381
Chapter III 1 Generally used pathophysiological methods 383
1 1 Biochemical analysis of bacteria 384
2 Infiltration of plant tissue 389
3 Analysis of plant tissue 390
4 Plant tissue and cell suspension cultures 391
5 Preparation of plant protoplasts 394
III 2 Factors for virulence of bacteria 403
2 1 Enzymes: soft rotting erwinias 405
2 Toxins 413
3 Extracellular polysaccharides (EPS) 418
4 Lipopolysaccharides (LPS) 423
5 Bacterial ice-nucleation activity 428
6 Siderophores and fluorescent pigments 434
7 Production of IAA and cytokinins 438
8 Flagella, motility, chemotaxis and helicity 441
9 Pili, fimbriae and fibrillae 444
111 3 Changes in the infected plant 455
3 1 Changes in membrane potential 455
3 Degradation of plant tissue 459
4 Changes in the sugar content 461
5 Ammonia production 463
6 Ethylene production 464
111 4 Mechanism of resistance 469
4 1 Hypersensitive reaction (HR) 469
2 Induced resistance 477
3 Phytoalexins 479
4 Encapsulation or agglutination of bacteria 483
5 Compartmentation of vascular infections 486
111 5 Bacterial genetics 495
5 2 Gene transfer methods 498
3 Mutagenesis 502
4 Plasmids 506
5 Construction of cosmid clone banks of phytobac-
terial genomes 510
6 Marker exchange (Homogenotization) 517
7 Plasmid vectors for transformation of plants 520
8 Sources of mistakes in pathological assays with
genetic mutants 526
111 6 Importance of phytopathogenic bacteria in biotech
nology 533
Textbooks 541
List of abbreviations 543
Media and Solutions 545
Subject index 547
|
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| illustrated | Illustrated |
| indexdate | 2024-07-09T17:07:43Z |
| institution | BVB |
| isbn | 9630549557 |
| language | Undetermined |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-005047853 |
| oclc_num | 633970952 |
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| physical | XIV, 568 S. Ill., graph. Darst. |
| publishDate | 1990 |
| publishDateSearch | 1990 |
| publishDateSort | 1990 |
| publisher | Akad. Kiadó |
| record_format | marc |
| spelling | Methods in phytobacteriology ed. by Z. Klement ... Budapest Akad. Kiadó 1990 XIV, 568 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Literaturangaben Bakterien (DE-588)4004296-0 gnd rswk-swf Pflanzen (DE-588)4045539-7 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Bakterien (DE-588)4004296-0 s Pflanzen (DE-588)4045539-7 s Methode (DE-588)4038971-6 s DE-604 Klement, Zoltán Sonstige oth HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=005047853&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Methods in phytobacteriology Bakterien (DE-588)4004296-0 gnd Pflanzen (DE-588)4045539-7 gnd Methode (DE-588)4038971-6 gnd |
| subject_GND | (DE-588)4004296-0 (DE-588)4045539-7 (DE-588)4038971-6 |
| title | Methods in phytobacteriology |
| title_auth | Methods in phytobacteriology |
| title_exact_search | Methods in phytobacteriology |
| title_full | Methods in phytobacteriology ed. by Z. Klement ... |
| title_fullStr | Methods in phytobacteriology ed. by Z. Klement ... |
| title_full_unstemmed | Methods in phytobacteriology ed. by Z. Klement ... |
| title_short | Methods in phytobacteriology |
| title_sort | methods in phytobacteriology |
| topic | Bakterien (DE-588)4004296-0 gnd Pflanzen (DE-588)4045539-7 gnd Methode (DE-588)4038971-6 gnd |
| topic_facet | Bakterien Pflanzen Methode |
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