A practical guide to molecular cloning:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York [u.a.]
Wiley
1988
|
| Ausgabe: | 2. ed. |
| Schriftenreihe: | A Wiley-interscience publication
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVII, 811 S. Ill., graph. Darst. 3 Beil. |
| ISBN: | 0471850713 0471850705 |
Internformat
MARC
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| 100 | 1 | |a Perbal, Bernard V. |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a A practical guide to molecular cloning |c Bernard Perbal |
| 250 | |a 2. ed. | ||
| 264 | 1 | |a New York [u.a.] |b Wiley |c 1988 | |
| 300 | |a XVII, 811 S. |b Ill., graph. Darst. |e 3 Beil. | ||
| 336 | |b txt |2 rdacontent | ||
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| 338 | |b nc |2 rdacarrier | ||
| 490 | 0 | |a A Wiley-interscience publication | |
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Datensatz im Suchindex
| _version_ | 1804120337797873664 |
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| adam_text | IMAGE 1
CONTENTS
1 LABORATORY EQUIPMENT NEEDED FOR MOLECULAR CLONING A FEW WORDS ABOUT
SAFETY
LABORATORY HOODS, 4 BIOLOGICAL SAFETY CABINETS, 4 GLOVES, 5 ULTRAVIOLET
LIGHT, 5
GERMICIDAL LIGHTS, 6 DETECTION OF DNA BY UV ILLUMINATION, 6 PHENOL, 6
FIRST AID, 6 REDISTILLATION OF PHENOL, 6 LIQUID SCINTILLATION COCKTAILS,
7 CHLOROFORM, 7 ISOAMYL ALCOHOL, 7 ETHYL ETHER, 8 DIETHYL PYROCARBONATE,
8 METHYLMERCURY, 8 PLASTICWARE AND CHEMICALS, 8 WORKING WITH 32
P-LABELED COMPOUNDS, 8 WORKING WITH KNOWN OR POTENTIAL CARCINOGENS, 8
BASIC METHODS IN MOLECULAR BIOLOGY
GEL ELECTROPHORESIS, 11 INTRODUCTION TO ELECTROPHORETIC THEORY, 11 SDS
GEL ELECTROPHORESIS: SEPARATION
OF PROTEINS ON THE BASIS OF MOLECULAR WEIGHT, 14 CONTINUOUS AND
DISCONTINUOUS BUFFER SYSTEMS, 19 TWO-DIMENSIONAL GEL ELECTROPHORESIS:
TO SEPARATE COMPLEX MIXTURES OF PROTEINS INTO MANY COMPONENTS, 23
ELECTROPHORETIC TRANSFER, 32 SEPARATION OF LOW MOLECULAR WEIGHT
PROTEINS BY POLYACRYLAMIDE GEL ELECTROPHORESIS, 34 SEPARATION METHODS IN
PREPARATIVE ULTRACENTRIFUGES, 38 DIFFERENTIAL CENTRIFUGATION, 38 DENSITY
GRADIENT CENTRIFUGATION, 38 SELECTION OF ROTORS, 40 PELLETING, 40
ISOPYCNIC SEPARATIONS (USUALLY USING
CESIUM CHLORIDE GRADIENTS), 40 RATE ZONAL SEPARATIONS (USUALLY USING
SUCROSE-GRADIENTS), 40
11
X;
1
4
IMAGE 2
XII
- CONTENTS
GEL FILTRATION WITH SEPHADEX, 43
CHEMICAL AND PHYSICAL PROPERTIES OF SEPHADEX, 43 ION EXCHANGE
CHROMATOGRAPHY, 44 THE THEORY OF ION EXCHANGE, 45
SEPHAROSE ION EXCHANGERS, 45 CHEMICAL AND PHYSICAL PROPERTIES, 45
NACS ION EXCHANGERS, 46 QUANTIFICATION OF PROTEINS, 46 LOWRY PROCEDURE,
46 BRADFORD PROCEDURE, 47 CHOICE OF BUFFERS, 49 AUTORADIOGRAPHY, 51
CHOOSING THE CORRECT EXPOSURE CONDITIONS, 51 ESTIMATING EXPOSURE TIMES,
52 FLUOROGRAPHY TECHNIGUES, 54 QUANTIFICATION OF RESULTS, 55
INTENSIFYING X-RAY FILMS, 55 TROUBLE-SHOOTING GUIDE, 57 PHYSICAL
PROPERTIES OF SOME RADIONUCLIDES USED IN MOLECULAR
CLONING, 59
PHYSICAL CONSTANTS OF NUCLEOSIDES AND NUCLEOTIDES, 59 PHENOL EXTRACTION
OF NUCLEIC ACIDS, 73 PREPARATION OF PHENOL FOR NUCLEIC
ACID EXTRACTIONS, 73 EXTRACTION OF NUCLEIC ACIDS, 74 ETHANOL
PRECIPITATION OF SMALL AMOUNTS OF NUCLEIC ACIDS, 76
SOME USEFUL HINTS AND UNITS, 79
4 USEFUL MODIFYING ENZYMES FOR MOLECULAR CLONING
T4DNALIGASE, 80 COLI DNA LIGASE, 82 T4 DNA POLYMERASE, 82 COLI DNA
POLYMERASES, 84
MODIFIED BACTERIOPHAGE T7 DNA POLYMERASE, 85 ALKALINE PHOSPHATASE, 86 T4
POLYNUCLEOTIDE KINASE, 86
TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE, 87
EXONUCLEASE III, 89 NUCLEASE BAL 31, 89 EXONUCLEASE VII, 90 TOBACCO ACID
PYROPHOSPHATASE, 90 RIBONUCLEASE H, 91 RIBONUCLEASE PHY I, 91 RNASECL3,
91 B. CEREUS RIBONUCLEASE, 91 RIBONUCLEASE PHY M, 91 RNASEUZ 91 RNASEPL,
92 RNASET2, 92 DNASEL, 92 S1 NUCLEASE, 92 MUNG BEAN NUCLEASE, 93
TOPOISOMERASE I, 93 TOPOISOMERASE II (DNA GYRASE), 93
GUANYLYLTRANSFERASE FROM VACCINIA
VIRUS, 94 POLYADENYLATE POLYMERASE OF E. COLI, 94 REVERSE TRANSCRIPTASE,
94 SP6 POLYMERASE, 96 T7 RNA POLYMERASE, 97
METHYLASES, 97 LISTING OF SOME MODIFYING ENZYMES COMMERCIALLY AVAILABLE,
102
5 RESTRICTION ENDONUCLEASES 107
6 VECTORS FOR MOLECULAR CLONING
PLASMID VECTORS, 201 BACTERIOPHAGE-DERIVED VECTORS, 234 BACTERIOPHAGE X,
234
COSMID VECTORS, 265 THE USE OF COSMIDS AS CLONING VEHICLES, 265 COSMID
VECTORS, 269
HOST STRAINS AND COSMID STABILITY, 269 IN VIVO PACKAGING OF COSMIDS, 270
RECOMBINATION SCREENING, 270 OTHER CLONING VECTORS, 273
PROCARYOTIC VECTORS, 273 YEAST CLONING VECTORS, 274
201
IMAGE 3
CONTENTS -
XIII
VIRAL VECTORS, 277
CLONING VECTORS FROM COMMERCIAL SOURCES, 278
7 PURIFICATION AND CHARACTERIZATION OF VECTOR AND PASSENGER DNA 297
ISOLATION OF PLASMID DNA USING CESIUM CHLORIDE GRADIENT CENTRIFUGATION,
297 RAPID PROCEDURE FOR ISOLATION OF PLASMID DNA, 304 REMOVAL OF RNA
SPECIES FROM DNA
PREPARATIONS, 307 CSTFA FOR ISOLATION AND SEPARATION OF NUCLEIC ACIDS BY
ISOPYCNIC CENTRIFUGATION, 309
PRODUCT DESCRIPTION, 310 SPECIFICATIONS FOR CSTFA, 310 INSTRUCTIONS FOR
USE, 310 PURIFICATION OF DNA RESTRICTION
FRAGMENTS, PLASMID DNA, AND CHROMOSOMAL DNA BY SEPHACRYL GEL
CHROMATOGRAPHY, 313 PREPARATION OF X PHAGE STOCKS AND
DNA, 313 TITRATION OF INITIAL SEED STOCK AND PREPARATION OF LARGE
STOCKS, 313 PURIFICATION OF HIGH MOLECULAR WEIGHT
DNA FROM EUCARYOTIC CELLS, 324
SEPARATION OF DNA FRAGMENTS BY ELECTROPHORESIS
AGAROSE GELS, 340 GEL CONCENTRATION, 340 PREPARATION OF THE AGAROSE
SOLUTION, 340 GEL BEDS, FOR HORIZONTAL
ELECTROPHORESIS, 340 COMBS, 344 VERTICAL AGAROSE GELS, 344 SEPARATION OF
DNA FRAGMENTS BY
PULSE AND REVERSED FIELD ELECTROPHORESIS, 344 STAINING OF DNA FRAGMENTS,
347 PHOTOGRAPHY OF DNA
FRAGMENTS, 349 POLYACRYLAMIDE GELS, 349 GEL CONCENTRATION, 349
PREPARATION OF GELS, 349
ANOMALOUS MOBILITIES OF DNA RESTRICTION FRAGMENTS ON POLYACRYLAMIDE
GELS, 350 STAINING OF DNA FRAGMENTS, 355 SIZE MARKERS FOR DNA FRAGMENTS
RUN IN
AGAROSE AND POLYACRYLAMIDE GELS, 356 AGAROSE GELS, 356 POLYACRYLAMIDE
GELS, 360 QUANTIFICATION OF DNA FRAGMENTS ON
AGAROSE GELS, 361
340
8 DIGESTION OF DNA WITH RESTRICTION ENDONUCLEASES
ENZYMATIC UNITS, 327 CONDITIONS FOR OPTIMAL ENZYMATIC ACTIVITY, 327
MULTIPLE DIGESTION OF DNA WITH
RESTRICTION ENDONUCLEASES, 331 STABILITY OF RESTRICTION ENDONUCLEASE
ACTIVITY DURING EXTENDED
DIGESTIONS, 335 PARTIAL DIGESTION OF DNA WITH RESTRICTION ENDONUCLEASES,
336 INCUBATION IN THE PRESENCE OF
ETHIDIUM BROMIDE, 338
327 10 ORDERING THE RESTRICTION DNA FRAGMENTS IN PHYSICAL MAPS
DOUBLE DIGESTION OF DNA FRAGMENTS, 363 MAPPING BY FURTHER DIGESTION OF
PURIFIED DNA FRAGMENTS, 363 MAPPING BY PARTIAL DIGESTION OF END-
LABELED DNA, 365 LABELING OF THE DNA ENDS, 365 ASYMMETRIC DIGESTION OF
LABELED DNA, 365
PARTIAL DIGESTION OF PURIFIED LABELED FRAGMENTS, 365
363
IMAGE 4
XIV
* CONTENTS
MAPPING RESTRICTION SITES WITH
BAL 31. 365 MAPPING BY TWO-DIMENSIONAL ANALYSIS OF END-LABELED DNA, 366
11 PURIFICATION OF DNA FRAGMENTS 368
PURIFICATION OF DNA FRAGMENTS FROM REGULAR AGAROSE GELS, 368
PURIFICATION OF DNA FRAGMENTS FROM LOW- MELTING AGAROSE GELS, 370
PURIFICATION OF DNA FROM AGAROSE OR
ACRYLAMIDE GELS USING DEAE PAPER, 372 EUTION OF DNA SPECIES FROM
POLYACRYLAMIDE GELS, 374
12 MODIFICATION OF DNA FRAGMENTS WITH COHESIVE TERMINI 375
MODIFICATION OF 3 AND 5 DNA TERMINI WITH DNA POLYMERASE, 375
MODIFICATION OF COHESIVE ENDS WITH 3 EXTENSION, 375
MODIFICATION OF COHESIVE ENDS WITH 5 EXTENSION, 377 DIGESTION OF DNA
WITH BAL 31 NUCLEASE, 377 ADDITION OF MOLECULAR LINKERS TO DNA
FRAGMENTS, 379 USE OF ADAPTERS FOR CLONING, 385
13 LIGATION 394
THEORY, 394 PRACTICE, 397
14 PROPAGATION OF RECOMBINANT DNA MOLECULES 402
REDUCTION OF TRANSFORMATION BACKGROUND DUE TO VECTOR
RECIRCULARIZATION-USE OF ALKALINE PHOSPHATASE, 402 CLONING IN TAILED
VECTOR, 405 TAILING CONDITIONS AND CALCULATIONS, 408 TRANSFORMATION OF
E. COLI STRAINS WITH
PLASMID DNA, 411
TRANSFORMATION OF COMPETENT CELLS FOLLOWING DIRECT LIGATION IN LOW
MELTING POINT AGAROSE, 416
15 CHARACTERIZATION OF RECOMBINANT CLONES 418
RAPID PURIFICATION OF RECOMBINANT PLASMID DNA, 418 DIRECT ANALYSIS OF
RECOMBINANT PLASMIDS BY VERTICAL ELECTROPHORESIS ON AGAROSE
GELS, 420 SCREENING OF RECOMBINANT BACTERIA ON NITROCELLULOSE FILTERS,
421 CAPILLARY TRANSFER OF DNA FRAGMENTS
TO NITROCELLULOSE MEMBRANES, 423 ELECTROPHORETIC DNA TRANSFER TO
NITROCELLULOSE MEMBRANES, 430 PREPARATION AND ACTIVATION OF
CHEMICALLY MODIFIED PAPERS FOR NUCLEIC ACIDS TRANSFER, 431 DNA TRANSFER
ON MODIFIED NYLON MEMBRANES, 436
DOT BLOTTING, 438 SLOT BLOTTING, 439 ANALYSIS OF RECOMBINANT DNA BY
HYBRIDIZATION WITH SPECIFIC PROBES, 439
REHYBRIDIZATION OF FILTERS, 445 DIRECT HYBRIDIZATION OF LABELED DNA TO
DNA IN AGAROSE GELS, 446 HYBRIDIZATION HISTOCHEMISTRY, 448
PREPARATION OF TISSUE, 448 PREPARATION OF SECTIONS, 448 CDNA PROBE
LABELING, 450 SYNTHESIS OF DNA PROBES, 450 WASHING CONDITIONS, 451
AUTORADIOGRAPHY, 451 STAINING, 451
PREPARATION OF WHOLE MOUSE SECTIONS, 451
16 LABELING OF NUCLEIC ACIDS 453
PREPARATION OF RADIOACTIVE PROBES, 453 NICK-TRANSLATION, 453 LABELING
DNA BY THE REPLACEMENT SYNTHESIS METHOD, 458
IMAGE 5
CONTENTS -
XV
END LABELING OF DNA FRAGMENTS, 461
PROCEDURES FOR NONRADIOACTIVE LABELING OF PROBES, 469 IN VITRO SYNTHESIS
OF LABELED RNA PROBES, 471
RADIOLABELED RNA PROBE SYNTHESIS WITH SP6, T3, AND T7 RNA POLYMERASES,
473 IN VITRO SYNTHESIS OF NONRADIOACTIVE
LABELED RNA PROBES WITH SP6, T3, AND T7 RNA POLYMERASES, 479
17 PREPARATION OF GENOMIC LIBRARIES
THEORETICAL STUDY OF THE FRACTION OF A LONG-CHAIN DNA THAT CAN BE
INCORPORATED IN A RECOMBINANT DNA PARTIAL-DIGEST LIBRARY, 480
THEORY, 480 COMPARISON OF THE ESTIMATES, 489 REPRESENTATION OF DNA
SEQUENCES IN RECOMBINANT DNA LIBRARIES PREPARED
BY RESTRICTION-ENZYME PARTIAL DIGESTION, 491 APPENDIX, 495 PREPARATION
OF LIBRARIES WITH X CHARON
PHAGES, 497 ISOLATION OF THE DNA TO BE CLONED, 497 PREPARATION OF X
CHARON ARMS, 501 LIGATION OF DIGESTED DNA TO X CHARON
ARMS, 503 LAMBDA IN VITRO PACKAGING SYSTEM, 503 AMPLIFICATION OF LAMBDA
LIBRARIES, 506
EFFICIENCY OF COMMERCIAL PACKAGING EXTRACTS, 508 PARAMETERS AFFECTING
EFFICIENCY OF THE PACKAGING REACTION, 508 SCREENING OF LAMBDA LIBRARIES
BY
HYBRIDIZATION WITH SPECIFIC PROBES, 510
18 PURIFICATION AND CHARACTERIZATION OF RNA SPECIES
PURIFICATION OF INTACT RNA SPECIES BY ULTRACENTRIFUGATION, 516
480
19
20
PURIFICATION OF RNA SPECIES FOR QUICK BLOT, 518 PREPARATION OF
CYTOPLASMIC RNA FROM EUCARYOTIC CELLS, 518
ISOLATION OF CYTOPLASMIC POLYADENYLATED RNA SPECIES, 522 ISOLATION OF
SPECIFIC RNA SPECIES, 524 FORMALDEHYDE GELS FOR RNA, 526 METHYL MERCURY
GELS FOR RNA, 527 GLYOXAL GELS FOR RNA, 530 SIZE MARKERS FOR RNA SPECIES
RUN IN
DENATURING AGAROSE GELS, 532 TRANSFER OF RNA TO NITROCELLULOSE, 533
METHYL MERCURY GELS, 533 GLYOXAL OR FORMALDEHYDE GELS, 534 TRANSFER OF
RNA TO DBM OR DPT
PAPER, 535 METHYL MERCURY GELS, 535 GLYOXAL OR FORMALDEHYDE GELS, 536
TRANSFER OF RNA TO NYLON
MEMBRANES, 537 HYBRIDIZATION TO MRNA IMMOBILIZED ON HYBOND MAP, 538
MAPPING RNAS BY DIGESTION OF DNA-RNA
HYBRIDS, 542 NUCLEASE S1 MAPPING OF DNA SEGUENCES HOMOLOGOUS TO RNA
SPECIES, 543
CLONING OF CDNA SPECIES 550
SYNTHESIS OF CDNA SPECIES WITH REVERSE TRANSCRIPTASE, 550 CLONING OF
CDNA SEQUENCES IN PLASMID VECTORS, 553
PREPARATION OF A CDNA LIBRARY IN LAMBDA GT11 VECTOR, 569
CHARACTERIZATION OF THE RECOMBINANT CLONES, 574
FREEZING BACTERIAL COLONIES DIRECTLY ON NITROCELLULOSE FILTERS, 576
SEQUENCING OF DNA 578
SEQUENCING OF DNA BY BASE-SPECIFIC CLEAVAGE, 578 LARGE-SCALE PRODUCTION
OF UNIGUE DNA FRAGMENTS, 578
IMAGE 6
XVI-
* CONTENTS
21
END LABELING OF THE DNA FRAGMENTS, 578 SEPARATION OF LABELED ENDS, 579
CHEMICAL CLEAVAGE REACTIONS, 580
DETAILED PROCEDURES, 580 MODIFIED MAXOM-GILBERT SEQUENCING PROCEDURE FOR
USE WITH OLIGODEOXYNUCIEOLIDES, 584 GEL ELECTROPHORESIS AND
AUTORADIOGRAPHY, 584 DNA CLONING IN M13 PHAGE FOR SEQUENCING, 585
PREPARATION OF VECTOR DNA, 585
PREPARATION OF DNA FRAGMENTS TO BE INSERTED, 593 LIGATION OF DNA
FRAGMENTS TO VECTOR DNA, 594
PREPARATION OF COMPETENT CELLS, TRANSFORMATION, 595 RAPID DELETION
SUBCSONMG SYSTEM FOR SEQUENCING, 599 CHARACTERIZATION OF M13 DNA
RECOMBINANTS, 605 SEQUENCING OF RECOMBINANT DNA OBTAINED WITH M13
CLONING, 610 PROCEDURE FOR PERFORMING DIDEOXY
SEQUENCING REACTIONS IN MICROTITER PLALES, 618 POLYACRYLAMIDE GEL
ELECTROPHORESIS, 619 AUTORADIOGRAPHY, 624
INTERPRETATION OF THE AUTORADIOGRAMS, 628 SOME COMMON PROBLEMS
ENCOUNTERED WHEN PERFORMING SEQUENCING.
TROUBLE SHOOTING GUIDE, 630 SEQUENCING WITH REVERSE TRANSCRIPTASE, 631
DIRECT SEQUENCING OF DNA FRAGMENTS
CLONED IN PLASMIDS, 634 SEQUENCING OF SINGLE STRANDED DNA IN BOTH
DIRECTIONS, 636
RNA SEQUENCING
CHEMICAL SPECIFIC CLEAVAGE OF RNA FOR SEQUENCING, 641 LABELING OF RNA 3
TERMINUS, 641
22
23
641
BASE SPECIFIC CLEAVAGE OF LABELED RNA, 642 ENZYMATIC SEQUENCING OF RNA,
647 PROCEDURES FOR ENZYMATIC
SEQUENCING, 649 USE OF REVERSE TRANSCRIPTASE FOR RNA SEQUENCING, 650
USE OF COMPUTERIZED PROGRAMS IN THE TREATMENT OF DNA SEQUENCE
INFORMATION 654
SEQUENCING PROGRAMS FOR SHOTGUN SEQUENCING, 655 MANUAL SHOTGUN
SEQUENCING, 655 AUTOMATIC SHOTGUN SEQUENCING, 655
SEARCH FOR OCCURRENCES OF NUCLEOTIDES AND BASE SEQUENCES IN DNA, 656
BASE FREQUENCIES, 656 RESTRICTION ENDONUCLEASE MAPS, 656
CONSENSUS SEQUENCES, 658 DETERMINATION OF SECONDARY STRUCTURES, 659
IDENTIFICATION OF PUTATIVE GENE
PRODUCTS, 659 PRIMARY STRUCTURE, MOLECULAR WEIGHT, 660 PROTEOLYTIC
DIGESTION, 660 LOCALIZATION OF POTENTIAL
ANTIGENIC EPITOPS, 661 LOCALIZATION OF SECONDARY STRUCTURES, 661
COMPARISON OF SEQUENCES, 666 COMPARISON OF SEQUENCES WITH THE
WHOLE CONTENT OF A DATA BANK, 675 DATA SOURCES, 675
LOCALIZED MUTAGENESIS
OLIGONUCLEOTIDE-DIRECTED MUTAGENESIS, 685 LINKERS MUTAGENESIS, 693
LINKER-SCANNING MUTAGENESIS, 693
TAB LINKER MUTAGENESIS, 695
685
IMAGE 7
CONTENTS -
XVA
RANDOM INTRODUCTION OF SINGLE BASE MUTATIONS IN A DEFINED DNA FRAGMENT,
707
SPECIFIC POINTS TO CONSIDER FOR THE SYNTHESIS OF THE MUTAGENIZED
OLIGONUCLEOTIDE, 708
24 METHODS FOR STUDYING D N A- PROTEIN INTERACTIONS
ANALYSIS OF PROTEIN-DNA COMPLEXES BY POLYACRYLAMIDE GEL ELECTROPHORESIS
(GEL RETARDATION), 719
DNASE I FOOTPRINTING, 723
LOCALIZATION OF CONTACT POINTS BY DIMETHYLSULFATE RESISTANCE, 725
ISOLATION OF DNA-PROTEIN COMPLEXES FOR THE ANALYSIS OF FOOTPRINTS
FOLLOWING DNASE I DIGESTION OR PROTECTION FROM DMS MODIFICATION, 728
25 EXPRESSION OF CLONED DNA SEQUENCES IN VITRO OR IN PROCARYOTIC AND
EUCARYOTIC CELLS
IN VITRO TRANSCRIPTION OF CLONED GENES, 734
IN VITRO TRANSLATION OF RNA EXPRESSED FROM CLONED GENES, 740 EXPRESSION
VECTORS FOR THE CHARACTERIZATION OF CLONED GENE
PRODUCTS, 751 EXPRESSION IN PROCARYOTIC
SYSTEMS, 751
VECTORS FOR MAMMALIAN CELLS, 758
719
731
RECOVERY OF INTACT GENE PRODUCTS FROM FUSION PROTEINS, 776
TRANSFECTION OF EUCARYOTIC CELLS WITH PURIFIED DNA, 780
TRANSFECTION OF CELLS FOLLOWING DEAE DEXLRAN TREATMENT, 780 DNA-CALCIUM
PHOSPHATE
COPRECIPITATION TECHNIQUE WITH CELL MONOLAYERS, 781 TRANSFECTION OF
CELLS IN SUSPENSION WITH DNA-CAICIUM PHOSPHATE
PRECIPITATES, 782 LIPOSOME-MEDIATED DNA TRANSFECTION, 783 DIRECT
TRANSFER OF PLASMID DNA FROM
BACTERIAL TO MAMMALIAN CELLS, 784 SELECTION OF CELLS EXPRESSING NEWLY
INTRODUCED DNA, 785 CONSTRUCTION OF COSMID LIBRARIES USED
TO TRANSFORM EUCARYOTIC CELLS, 786 DETECTION OF POLYPEPTIDE SYNTHESIS BY
IMMUNE PRECIPITATION, 788 DETECTION OF POLYPEPTIDE SYNTHESIS BY
IMMUNOBLOTTING, 792
EPILOGUE: IF YOU WANT AVA I TO MEET BAM H I . .. 795
APPENDIX: ADDRESSES OF MAIN OFFICES FOR SUPPLIERS CITED IN THIS MANUAL
798
INDEX 801
|
| any_adam_object | 1 |
| author | Perbal, Bernard V. |
| author_facet | Perbal, Bernard V. |
| author_role | aut |
| author_sort | Perbal, Bernard V. |
| author_variant | b v p bv bvp |
| building | Verbundindex |
| bvnumber | BV006113533 |
| classification_rvk | WC 4350 WC 4400 WC 4460 WG 2900 WG 3450 |
| ctrlnum | (OCoLC)246886681 (DE-599)BVBBV006113533 |
| discipline | Biologie |
| edition | 2. ed. |
| format | Book |
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| id | DE-604.BV006113533 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T16:40:29Z |
| institution | BVB |
| isbn | 0471850713 0471850705 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-003862156 |
| oclc_num | 246886681 |
| open_access_boolean | |
| owner | DE-703 DE-355 DE-BY-UBR DE-29 DE-29T DE-188 |
| owner_facet | DE-703 DE-355 DE-BY-UBR DE-29 DE-29T DE-188 |
| physical | XVII, 811 S. Ill., graph. Darst. 3 Beil. |
| publishDate | 1988 |
| publishDateSearch | 1988 |
| publishDateSort | 1988 |
| publisher | Wiley |
| record_format | marc |
| series2 | A Wiley-interscience publication |
| spelling | Perbal, Bernard V. Verfasser aut A practical guide to molecular cloning Bernard Perbal 2. ed. New York [u.a.] Wiley 1988 XVII, 811 S. Ill., graph. Darst. 3 Beil. txt rdacontent n rdamedia nc rdacarrier A Wiley-interscience publication Methode (DE-588)4038971-6 gnd rswk-swf Genklonierung (DE-588)4123275-6 gnd rswk-swf DNS-Klonierung (DE-588)4250249-4 gnd rswk-swf Klonierung (DE-588)4192480-0 gnd rswk-swf Klonierung (DE-588)4192480-0 s Methode (DE-588)4038971-6 s DE-604 Genklonierung (DE-588)4123275-6 s 1\p DE-604 DNS-Klonierung (DE-588)4250249-4 s 2\p DE-604 SWB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=003862156&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Perbal, Bernard V. A practical guide to molecular cloning Methode (DE-588)4038971-6 gnd Genklonierung (DE-588)4123275-6 gnd DNS-Klonierung (DE-588)4250249-4 gnd Klonierung (DE-588)4192480-0 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4123275-6 (DE-588)4250249-4 (DE-588)4192480-0 |
| title | A practical guide to molecular cloning |
| title_auth | A practical guide to molecular cloning |
| title_exact_search | A practical guide to molecular cloning |
| title_full | A practical guide to molecular cloning Bernard Perbal |
| title_fullStr | A practical guide to molecular cloning Bernard Perbal |
| title_full_unstemmed | A practical guide to molecular cloning Bernard Perbal |
| title_short | A practical guide to molecular cloning |
| title_sort | a practical guide to molecular cloning |
| topic | Methode (DE-588)4038971-6 gnd Genklonierung (DE-588)4123275-6 gnd DNS-Klonierung (DE-588)4250249-4 gnd Klonierung (DE-588)4192480-0 gnd |
| topic_facet | Methode Genklonierung DNS-Klonierung Klonierung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=003862156&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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