Ultrastructure techniques for microorganisms:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York u. a.
Plenum Pr.
1986
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XIV, 533 S. Ill., graph. Darst. |
| ISBN: | 0306422514 |
Internformat
MARC
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| 245 | 1 | 0 | |a Ultrastructure techniques for microorganisms |c ed. by Henry C. Aldrich ... |
| 264 | 1 | |a New York u. a. |b Plenum Pr. |c 1986 | |
| 300 | |a XIV, 533 S. |b Ill., graph. Darst. | ||
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| 650 | 4 | |a Micro-organismes - Morphologie | |
| 650 | 4 | |a Microscopie - Technique | |
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| 650 | 4 | |a Ultrastructure (Biologie) | |
| 650 | 4 | |a Electron microscopy |x Technique | |
| 650 | 4 | |a Microorganisms |x Morphology | |
| 650 | 4 | |a Microscopy |x Technique | |
| 650 | 4 | |a Microscopy, Electron |x methods | |
| 650 | 4 | |a Ultrastructure (Biology) | |
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Datensatz im Suchindex
| _version_ | 1804120246746873856 |
|---|---|
| adam_text | Ultrastructure
Techniques for
Microorganisms
Edited by
Henry C Aldrich
Department of Microbiology and Cell Science
University of Florida
Gainesville, Florida
and
William J Todd
Department of Veterinary Science
Louisiana Agricultural Experiment Station
Louisiana State University Agricultural Center
and Department of Veterinary Microbiology and Parasitology
School of Veterinary Medicine
Louisiana State University
Baton Rouge, Louisiana
Plenum Press • New York and London
Contents
Chapter 1
Preparation of Microfungi for Scanning Electron Microscopy
Garry T Cole
1 Introduction 1
2 Microfungi in Pure Culture 2
3 Fungus-Natural Substrate Associations 3
4 Correlation of Morphological Data 27
5 Limitations of Preparatory Procedures 32
6 References 38
Chapter 2
Low-Temperature Scanning Electron Microscopy
Alan Beckett and Nick D Read
1 Introduction 45
2 Historical Review 48
3 The Apparatus and Methods Used 51
4 A Comparative Evaluation of the Technique 59
5 The Value and Importance of the Technique 79
6 Conclusions 82
7 References 83
Chapter 3
Effects of Specimen Preparation on the Apparent Ultrastructure of
Microorganisms
William J Todd
1 Specimen Preparation for Examination of Thin Sections 87
2 Fixation Additives to Improve Detection of Microbial Structures 90
ix
if Contents
3 Mesosomes and Fixation 92
4 Images of Gonococcal Pili 93
5 References 99
Chapter 4
Secrets of Successful Embedding, Sectioning, and Imaging
H C Aldrich and H H Mollenhauer
1 Introduction 101
2 Historical Aspects 102
3 Fixation 103
4 Shrinkage 104
5 Flat Embedment for Light Microscope Selection of Cells 108
6 Other Embedding Tricks 108
7 Microtomy 108
8 Stability of Epoxy Resins 109
9 Tissue and Section Contaminants 113
10 Resin Modifiers for Improving Sectioning 120
11 Image 123
12 Evaluation of Diamond Knives 125
13 Safety 128
14 Appendix: Suggested Reading 129
15 References 130
Chapter 5
Computer-Aided Reconstruction of Serial Sections
David L Balkwill
1 Introduction 133
2 Literature Review 135
3 Serial Sectioning 138
4 Obtaining the Ultrastructural Data for Computer-Aided
Reconstruction 141
5 Entry of Ultrastructural Data into the Computer System 143
6 Production and Manipulation of Computer-Generated
Reconstructions 147
7 Conclusions 157
8 References 158
Contents xi
Chapter 6
Electron Microscopy of Nucleic Acids
Claude F Garon
1 Introduction and History 161
2 Sample Mounting Techniques 164
3 Sample Preparation Requirements 168
4 DNA Analyses 169
5 RNA Analyses 174
6 DNA-RNA Interactions 176
7 Digitizing and Computer Analysis 179
8 Summary and Conclusions 180
9 References 180
Chapter 7
Freeze-Substitution of Fungi
Harvey C Hoch
1 Introduction 183
2 Fixation Methods Used for Fungi 183
3 Freeze-Substitution 185
4 Recommended Protocol for Preserving Fungi Using Quenching
Methodologies 187
5 Typical Results and Criteria for Good Preservation 194
6 Advantages of Using Freeze-Substitution 203
7 Disadvantages of Using Freeze-Substitution 205
8 Cell Systems to Be Studied Using Freeze-Substitution 208
9 References * 209
Chapter 8
Freeze-Fracture (-Etch) Electron Microscopy
Russell L Chapman and L Andrew Staehelin
1 Introduction 213
2 A Brief History 214
3 Methods and Recommendations 215
4 Typical Results 237
5 References 237
ni I Contents
Chapter 9
Preparation of Freeze-Dried Specimens for Electron Microscopy
Thomas H Giddings, Jr , and George P Wray
1 Introduction 241
2 Historical Origins 242
3 Methods and Instrumentation 242
4 Examples of Results 252
5 Concluding Remarks 262
6 References 263
Chapter 10
Low-Temperature Embedding
Barbara L Armbruster and Edward Kellenberger
1 Introduction 267
2 Technical Procedures 270
3 Results 275
4 Perspectives 290
5 References 291
Chapter 11
High-Voltage Electron Microscopy
Sandra A Nierzwicki-Bauer
1 Introduction 297
2 The High-Voltage Electron Microscope 298
3 Theoretical Advantages and Disadvantages of High Accelerating
Voltages 302
4 Examination of Thick Sections 305
5 Examination of Whole Cells 320
6 Conclusions 327
7 References 328
Chapter 12
Computer Analysis of Ordered Microbiological Objects
Murray Stewart
1 Introduction 333
2 Diffraction Patterns and Fourier Transforms 334
3 Image Processing 340
Contents ; xiii
4 Three-Dimensional Reconstruction 359
5 Conclusions 361
6 References 362
Chapter 13
Digitizing and Quantitation
Edwin S Boatman
1 Introduction 365
2 Historical Background 365
3 Definitions and Symbols 367
4 Digitizing Tablets, Image Analyzers, and Point-Counting 367
5 Methods of Measurement 370
6 References 397
7 Suggested Reading 398
Chapter 14
Localization of Carbohydrate-Containing Molecules
Gregory W Erdos
1 Introduction 399
2 Cationic Reagents 400
3 PAS-Based Reactions 405
4 Lectins 407
5 Glycosidic Enzymes 413
6 Carbohydrate-Specific Antibodies 415
7 References : 416
Chapter 15
Cytochemical Techniques for the Subcellular Localization
of Enzymes in Microorganisms
Martha J Powell
1 Introduction 421
2 Ion Capture of Enzyme Product 424
3 Ferricyanide Reduction 433
4 Diaminobenzidine Oxidation 437
5 Conclusions 441
6 References 441
xiv Contents
Chapter 16
Localization of Nucleic Acids in Sections
H C Aldrich and Sylvia E Coleman
1 Introduction 447
2 Selective Stains 447
3 Feulgen-Type Stains for DNA 449
4 Distinguishing between DNA and RNA 452
5 Gold-Labeled Antibody Staining of Specific Types of RNA 457
6 Gold-Labeled DNase and RNase 457
7 Extraction Methods 461
8 In Situ Hybridization 462
9 Gautier s HAPTA Technique 464
10 Summary and Perspectives 464
11 References 465
Chapter 17
Colloidal Gold Labels for Immunocytochemical Analysis
of Microbes
John Smit and William J Todd
1 Introduction 469
2 Production of Gold Colloids 477
3 Coupling Proteins to the Colloidal Gold Sols 483
4 Types of Labeling Made Possible Using Colloidal Gold Complexes 492
5 Application—Analysis of Microbial Surfaces and Surface Structure 496
6 Application—Analysis of Internal Structures and Components 498
7 Applications in Other Types of Microscopy 508
8 Concluding Comments 510
9 References 510
Chapter 18
X-Ray Microanalysis
H C Aldrich
1 Introduction 517
2 Principles 517
3 Instruments and Their Capabilities 519
4 Specimen Preparation 520
5 Recommendations and Outlook 524
6 References 524
Index 527
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| spelling | Ultrastructure techniques for microorganisms ed. by Henry C. Aldrich ... New York u. a. Plenum Pr. 1986 XIV, 533 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Electron Microscopy cabt Microorganisms cabt Techniques cabt Micro-organismen gtt Micro-organismes - Morphologie Microscopie - Technique Microscopie électronique - Technique Ultrastructure (Biologie) Electron microscopy Technique Microorganisms Morphology Microscopy Technique Microscopy, Electron methods Ultrastructure (Biology) Elektronenmikroskopie (DE-588)4014327-2 gnd rswk-swf Ultrastruktur (DE-588)4061568-6 gnd rswk-swf Mikroorganismus (DE-588)4039226-0 gnd rswk-swf Mikroorganismus (DE-588)4039226-0 s Elektronenmikroskopie (DE-588)4014327-2 s DE-604 Ultrastruktur (DE-588)4061568-6 s Aldrich, Henry C. Sonstige oth HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=003798904&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Ultrastructure techniques for microorganisms Electron Microscopy cabt Microorganisms cabt Techniques cabt Micro-organismen gtt Micro-organismes - Morphologie Microscopie - Technique Microscopie électronique - Technique Ultrastructure (Biologie) Electron microscopy Technique Microorganisms Morphology Microscopy Technique Microscopy, Electron methods Ultrastructure (Biology) Elektronenmikroskopie (DE-588)4014327-2 gnd Ultrastruktur (DE-588)4061568-6 gnd Mikroorganismus (DE-588)4039226-0 gnd |
| subject_GND | (DE-588)4014327-2 (DE-588)4061568-6 (DE-588)4039226-0 |
| title | Ultrastructure techniques for microorganisms |
| title_auth | Ultrastructure techniques for microorganisms |
| title_exact_search | Ultrastructure techniques for microorganisms |
| title_full | Ultrastructure techniques for microorganisms ed. by Henry C. Aldrich ... |
| title_fullStr | Ultrastructure techniques for microorganisms ed. by Henry C. Aldrich ... |
| title_full_unstemmed | Ultrastructure techniques for microorganisms ed. by Henry C. Aldrich ... |
| title_short | Ultrastructure techniques for microorganisms |
| title_sort | ultrastructure techniques for microorganisms |
| topic | Electron Microscopy cabt Microorganisms cabt Techniques cabt Micro-organismen gtt Micro-organismes - Morphologie Microscopie - Technique Microscopie électronique - Technique Ultrastructure (Biologie) Electron microscopy Technique Microorganisms Morphology Microscopy Technique Microscopy, Electron methods Ultrastructure (Biology) Elektronenmikroskopie (DE-588)4014327-2 gnd Ultrastruktur (DE-588)4061568-6 gnd Mikroorganismus (DE-588)4039226-0 gnd |
| topic_facet | Electron Microscopy Microorganisms Techniques Micro-organismen Micro-organismes - Morphologie Microscopie - Technique Microscopie électronique - Technique Ultrastructure (Biologie) Electron microscopy Technique Microorganisms Morphology Microscopy Technique Microscopy, Electron methods Ultrastructure (Biology) Elektronenmikroskopie Ultrastruktur Mikroorganismus |
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