Methods in stomatal research:
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Harlow, Essex
Longman Scientific & Technical u.a.
1990
|
| Ausgabe: | 1. publ. |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 233 S. |
| ISBN: | 0582034833 |
Internformat
MARC
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| 245 | 1 | 0 | |a Methods in stomatal research |c Jonathan D. B. Weyers ; Hans Meidner |
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Datensatz im Suchindex
| _version_ | 1804118718722080768 |
|---|---|
| adam_text | Methods in
Stomatal Research
Jonathan D B Weyers
University of Dundee, UK
Hans Meidner
University of Stirling, UK
Longman
Scientific amp;
Technical
Contents
Page
1 Introduction 1
2 Plant Material 3
2 1 STOMATAL ANATOMY AND THE MECHANISM OF GUARD CELL MOVEMENTS 3
211 Stomatal Structure and Frequency 3
212 Mechanics of Guard Cell Deformation and Changes in Pore Dimensions 6
2 13 Outline of Solute Relations and Biochemistry of Stomatal Cells 9
214 Relevant aspects of Leaf and Epidermal Morphology 12
12 VARIABILITY IN STOMATAL AND EPIDERMAL CHARACTERISTICS 16
221 Differences Among Plants or One Species 17
122 Differences Among Leaves on a Single Plant 17
122 Variation Within Individual Mature Leaves 18
13 SELECTION OF SUITABLE SPECIES 21
23 1 Selection Criteria and The Possibility of Bias 21
2 4 PROVISION OF PLANT MATERIAL FOR STUDY 24
241 Availability of Plants 24
2 42 Cultivation of Plant Material 24
2 43 Sampling of Experimental Material 26
2 5 SUMMARY OF FACTORS AFFECTING STOMATAL MOVEMENTS 26
251 Environmental Factors Affecting Stomata 27
252 Endogenous Influences on Stomatal Behaviour 31
2 53 Interactions Among Factors 33
2 6 EXPERIMENTAL REGIMES 34
261 Preparation of Plant Material for Experimentation 34
262 Special Pretreatments to Plant Material 35
2 63 Description of Experimental Regimes and Plant Material 36
3 Theoretical Background 38
3 1 INTRODUCTION 38
3 2 PRINCIPLES OF MASS TRANSFER 38
321 The Energy Requirement for Molecular Movement 38
322 Types of Flux 39
3 23 Driving Forces 39
324 Conductances and Resistances 41
33 PATHS OF AIR AND VAPOUR MOVEMENT 44
33 1 Fluxes through Stomata 44
33 2 Fluxes over Leaves 46
333 Fluxes within Canopies and Plant Communities 48
3 4 OVERVIEW OF METHODS 48
341 Types of Research 48
342 Quantifying and Comparing Stomatal Responses SI
3S ESTIMATING CONDUCTANCES 55
351 Composite, Total and Leaf Conductances 55
352 Stomatal Conductance perse 56
3 53 The Conductance of Non-stomatal Components of The Pathway 58
354 Representative Conductance Values 61
4 Gravimetric and Potometric Methods 63
4 1 INTRODUCTION AND BACKGROUND 63
42 GRAVIMETRIC DETERMINATIONS 64
421 Plant Material with a Water Supply 64
422 Single Drying Leaves 65
4 23 Lysimeters 69
43 POTOMETERS 72
4 4 ABSORPTION LAG AND PLANT CAPACITANCE 75
5 Mass-Flow Porometers 78
5 1 INTRODUCTION AND BACKGROUND 78
52 PROBLEMS OF MASS-FLOW POROMETRY 81
521 Attachment and Sealing of Porometer Cups 81
522 Changes in Air Viscosity 81
523 Experimental Material 83
524 Calibration of Mass-flow Porometers in Diffusion Conductance Units 83
53 A SELECTION OF MASS-FLOW POROMETERS 85
53 1 Simple Instruments for Field Use 86
53 2 Complex Instruments for Field and Laboratory Use 88
5 4 LIQUID-FLOW POROMETRY USING EPIDERMAL STRIPS 94
6 Diffusion Porometers 96
6 1 INTRODUCTION AND BACKGROUND 96
62 INERT GAS DIFFUSION METHODS 96
63 WATER VAPOUR DIFFUSION METHODS 99
63 1 Early Techniques 99
63 2 Later Developments 100
633 Sensor Element Diffusion Porometers 102
63 4 Cuvette Gas-analysis Systems 105
7 Determining Stomatal Aperture 106
7 1 INTRODUCTION 106
12 MICROSCOPIC METHODS 106
721 General Aspects of Technique 106
122 Measurements on Intact Leaves 108
123 Preserved or Fixed Epidermal Strips 109
724 Micro-Relief Methods 111
73 INFILTRATION OF LIQUIDS INTO LEAVES 117
73 1 Background and Theory 117
73 2 The Method of Alvim and Havis (1954) 119
8 Responses of Stomata in Leaf Pieces and Discs 121
8 1 INTRODUCTION 121
$2 METHODS OF EXPERIMENTING WITH LEAF DISCS 122
821 Discs Floating on Water or Supplied with Water via Their Cut Edges 122
822 Submerged Discs 123
8 23 Use of the Special Leaf Disc Porometer Cup 125
83 ADVANTAGES AND DISADVANTAGES OF USING LEAF DISCS 126
9 Epidermal Strips 129
9 1 INTRODUCTION 129
92 METHODS 129
921 Choice and Preparation of Experimental Material 129
922 Removal Of Epidermal Strips 135
9 23 Pretreatments To Epidermal Strips 140
924 Incubation Of Epidermal Strips In Vtoo 143
92S Viability Tests 151
9-2 6 Collection And Analysis Of Data 151
93 USE OF EPIDERMAL STRIPS IN SPECIALISED EXPERIMENTS 153
9 4 THE CONTRIBUTION OF WORK WITH EPIDERMAL STRIPS TO KNOWLEDGE 155
ABOUT STOMATA
10 Protoplasts 156
10 1 BACKGROUND 156
10 2 METHODS 157
10 2 1 Starting Material 157
1022 Isolation of GCPs 159
1023 Purification of GCPs 163
10 2 4 Viability Tests 167
10 2 5 Techniques for Experimentation 167
103 OSMOTIC RELATIONS OF GCPs 169
103 1 Theoretical and Practical Considerations: GCPs as osmometers 169
103 2 Measurement and Analysis of Changes in Osmotic Parameters of GCPs 171
10 4 USE OF GCPs TO PROBE THE STOMATAL MECHANISM 174
10 4 1 Localisation and Quantification of Responses 174
10 42 Biophysical and Biochemical Studies 177
10 5 EPIDERMAL AND SUBSIDIARY CELL PROTOPLASTS 177
10 5 1 Methods 177
10 5 2 Responses of ECPs 178
11 Guard Cells and Sub-cellular Fractions 179
11 1 INTRODUCTION 179
112 MICROSURGICAL TECHNIQUES FOR ISOLATING GUARD CELL PAIRS 179
11 2 1 Living Material 179
11 2 2 Freeze-dried Material 180
113 INVESTIGATIONS AT THE SUB-CELLULAR LEVEL 181
113 1 The Guard Cell Wall and Apoplast Solution 181
113 2 Sub-protoplasts of Guard Cells 183
1133 The Guard Cell Plasmalemma 184
113 4 The Cytoplasm, Chloroplasts and Mitochondria of Guard Cells 187
113 5 TheVacuole 189
12 Appendix 192
12 1 UNITS, SYMBOLS AND ABBREVIATIONS 192
12 1 1 Base Units, Relevant Derived Units and Preflxes or the Systeme 192
Internationale (SI)
12 1 2 Symbols and Abbreviations 193
12 2 DATA TABLES AND DESCRIPTIONS OF METHODS AND FORMULTATIONS 195
122 1 Brodie s Solution for Manometers 195
12 2 2 Osmotica and their Use with Stomata 195
12 23 Properties or Water Vapour 197
12 2 4 Starch Determinations with Heath s Reagent 198
12 2 5 Washers for Porometers 199
12 2 6 Viability Tests for Stomatal Work 199
123 SUPPLIERS FOR CHEMICALS AND EQUIPMENT 200
References 202
Index 222
|
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| dewey-ones | 581 - Specific topics in natural history of plants |
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| dewey-search | 581.4/2 |
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| discipline | Biologie |
| edition | 1. publ. |
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| indexdate | 2024-07-09T16:14:45Z |
| institution | BVB |
| isbn | 0582034833 |
| language | English |
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| physical | 233 S. |
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| spelling | Weyers, Jonathan D. B. Verfasser aut Methods in stomatal research Jonathan D. B. Weyers ; Hans Meidner 1. publ. Harlow, Essex Longman Scientific & Technical u.a. 1990 233 S. txt rdacontent n rdamedia nc rdacarrier Stomata gtt Stomata Research Methodology Forschungsmethode (DE-588)4155046-8 gnd rswk-swf Spaltöffnung (DE-588)4182035-6 gnd rswk-swf Spaltöffnung (DE-588)4182035-6 s Forschungsmethode (DE-588)4155046-8 s DE-604 Meidner, Hans Verfasser aut HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=002824335&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Weyers, Jonathan D. B. Meidner, Hans Methods in stomatal research Stomata gtt Stomata Research Methodology Forschungsmethode (DE-588)4155046-8 gnd Spaltöffnung (DE-588)4182035-6 gnd |
| subject_GND | (DE-588)4155046-8 (DE-588)4182035-6 |
| title | Methods in stomatal research |
| title_auth | Methods in stomatal research |
| title_exact_search | Methods in stomatal research |
| title_full | Methods in stomatal research Jonathan D. B. Weyers ; Hans Meidner |
| title_fullStr | Methods in stomatal research Jonathan D. B. Weyers ; Hans Meidner |
| title_full_unstemmed | Methods in stomatal research Jonathan D. B. Weyers ; Hans Meidner |
| title_short | Methods in stomatal research |
| title_sort | methods in stomatal research |
| topic | Stomata gtt Stomata Research Methodology Forschungsmethode (DE-588)4155046-8 gnd Spaltöffnung (DE-588)4182035-6 gnd |
| topic_facet | Stomata Stomata Research Methodology Forschungsmethode Spaltöffnung |
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