Yeast genetic engineering:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Boston u.a.
Butterworths
1989
|
| Schriftenreihe: | Biotechnology series
13 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Nebent.: Yeast genetic engineering / Philip J. Barr ; Anthony J. Brake ; Pablo Valenzuela |
| Beschreibung: | XVIII, 354 S. graph. Darst. |
| ISBN: | 0409901172 |
Internformat
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Datensatz im Suchindex
| _version_ | 1804118383934832640 |
|---|---|
| adam_text | Yeast Genetic Engineering
Edited by
Philip J Barr
Chiron Corporation
Emeryville, California
Anthony J Brake
Chiron Corporation
Emeryville, California
Pablo Valenzuela
Chiron Corporation
Emeryville, California
Butterworths
Boston London Singapore Sydney Toronto Wellington
!CONTENTS!
Preface xvii
1 Molecular Genetics of Galactose Metabolism in Yeast 1
Toshio Fukasawa and Yasuhisa Nogi
1 1 Upstream Activating Sequences 3
1 2 Functional Domains of Regulatory Proteins GALA
and GAL80 5
1 3 Autogenous Regulation of GAL80 9
1 4 Factors Determining the Expression Level of
Galactose-Inducible Genes 10
1 5 Genes Involved in Glucose Repression Specific for
Galactose-Inducible Genes 11
1 6 Other Regulatory Proteins 12
1 7 Prospects 15
References 16
2 The Lactose-Galactose Regulon of Kluyveromyces lactis 19
Robert C Dickson and Michael I Riley
2 1 Molecular Genetic Techniques 20
2 2 The Lactose-Galactose Regulon: General Background 22
xi
Xil Contents
2 3 Structural Genes: Molecular Cloning and Physical
Characterization 24
2 4 Cis-Acting Regulatory Elements 30
2 5 Transacting Regulatory Elements 32
2 6 Comparison of A, lactis and S cerevisiae 35
27A Model for the Lactose-Galactose Regulon 36
References 38
3 Phosphorus Regulation in Yeast 41
Akio Toh-e
3 1 Cloning of Structural Genes 42
3 2 Cloning of Regulatory Genes 46
3 3 Conclusion 50
References 50
4 Fission Yeast 53
Masayuki Yamamoto
4 1 Molecular-Genetic Overview of
Schizosaccharomyces pombe 53
4 2 Cell Cycle Control 55
4 3 Life Cycle Control 57
4 4 Biotechnological Aspects 60
References 62
5 The Role of Yeast mRNA Sequences and
Structures in Translation 65
John M Clements, Thomas Laz, and Fred Sherman
5 1 Yeast Requires an AUG Codon for Efficient
Initiation of Translation 67
5 2 Consensus Sequences Adjacent to the Initiation
AUG Codon and Context Effects 70
5 3 Special Sequences in the 5 Noncoding Region
Are Not Required 70
5 4 Secondary Structures Diminish Translation 72
5 5 Selection of the AUG Codon for Initiation of Translation 73
5 6 Some Yeast Genes Exhibit Translational Control 74
5 7 The Relationships between Translation and mRNA
Stability 77
5 8 Codon Usage in Yeast 78
5 9 Conclusions 79
References 80
Contents xfii
6 Regulated Transcriptional Systems for the Production of
Proteins in Yeast: Regulation by Carbon Source 83
Jeffrey R Shuster
6 1 The Physiology of Yeast Heterologous Expression
Systems 87
6 2 The Carbon Source-Based Systems 88
6 3 Non-Saccharomyces Systems 102
6 4 Overall Conclusions and Future Directions 103
References 104
7 Targeting of Proteins for Degradation 109
Alexander Varshavsky, Andreas Bachmair, Daniel Finley,
David K Gonda, and Lngrid Wiinning
7 1 Ubiquitin Fusion Proteins as In Vivo
Proteolytic Substrates 112
7 2 The N-End Rule 124
7 3 Possible Role of the N-End Rule Pathway in the
Turnover of Long-Lived Proteins 127
7 4 Function of Posttranslational Conjugation of Amino
Acids to Amino Termini of Proteins: Primary and
Secondary Destabilizing Amino Acids 128
7 5 Amino-Terminal versus Interior Targeting in
Selective Protein Turnover 131
7 6 In Vitro Analysis of the N-End Rule Pathway 133
7 7 Current Problems in Amino-Terminal Targeting 135
References 141
8 The Yeast Retrotransposon Ty:
Molecular Genetics and Exploitation 145
Susan M Kingsman, Sally E Adams, and Alan J Kingsman
8 1 Genetic Organization of Ty 146
8 2 Genomic Distribution and Recombination 148
8 3 Ty Transcription 149
8 4 Ty-Mediated Gene Disruption and Gene Activation 151
8 5 Proteins Encoded by Ty 152
8 6 Ty Viruslike Particles 152
8 7 Ty Transposition: A Model 153
8 8 Ty Variability 154
89A Comparison of Ty and Retroviral Proviruses 156
8 10 The Relationship of Ty to Other Retroelements 157
8 11 Exploitation of Ty: Multicopy Integrative Vectors 158
xiv Contents
8 12 Exploitation of Ty: Polyvalent Particulate Antigen
Carriers 159
References 161
9 Propagation and Expression of Genes in
Yeast Using 2-Micron Circle Vectors 165
Karen A Armstrong, Tapan Som, Frederic C Volkert,
Alan Rose, and James R 3roach
9 1 The Yeast Plasmid 2-Micron Circle 166
9 2 Standard 2-Micron Circle-Based Vectors 170
9 3 Yeast Vectors Devoid of Bacterial Plasmid Sequences 177
9 4 Vectors for High-Copy Propagation 179
9 5 Vectors for Inducible, High-Level Expression 184
Appendix: Vector Constructions 186
References 190
10 Heterologous Gene Expression in Yeast 193
Albert Hinnen, Bernd Meyhack, and Jutta Heim
10 1 2-Micron-Derived Yeast Expression Vectors 193
10 2 The Use of PH05 and PT/05-Derived Promoters
for Regulated Heterologous Expression 200
10 3 Use of the Secretory Pathway for the Production of
Complex Proteins 204
References 211
11 Yeast Mutants with Increased Secretion Efficiency 215
Donald T Moir
11 1 Secretion Capacity of Saccharomyces cerevisiae 216
11 2 Limitations to Mammalian Protein Secretion from Yeast 217
11 3 Mutational Approaches to Increase Heterologous
Protein Secretion 221
11 4 Properties of the Mutants—Effects on Heterologous
Protein Secretion 224
11 5 Conclusions and Future Directions 229
References 230
12 Glycosylation of Heterologous Proteins in
Saccharomyces cerevisiae
Michael A Innis
12 1 Yeast Glycosylation
12 2 Secretion Pathway
Contents XV
12 3 Glycosylation and Secretion of Aspergillus awamori
Glucoamylase from Yeast 237
12 4 Hyperglycosylation of Trichoderma reesei Cellulases 238
12 5 Secretory Leaders for Secretion of Foreign Proteins 239
12 6 Recognition of Heterologous Glycosylation Signals 240
12 7 Prospects for Yeast-Secreted Therapeutic Glycoproteins 241
References 245
13 Cellulolytic Yeast Strains and Their Application 247
Merja Penttild, Pdivi Lehtovaara, and Jonathan Knowles
13 1 Cellulolytic Enzymes 248
13 2 Structure of the Cellulases 249
13 3 Expression of Cellulase Genes in Yeast 251
13 4 Secretion of the Trichoderma Cellulases in Yeast 253
13 5 Cellulolytic Activity of the Recombinant Yeasts
Producing Trichoderma Cellulases 258
13 6 Application of Cellulolytic Yeasts in Brewing 259
13 7 Conclusions and Future Prospects 263
References 264
14 Secretion of Heterologous Proteins Directed by the
Yeast a-Factor Leader 269
Anthony J Brake
14 1 Biosynthesis and Secretion of Yeast a-Factor 270
14 2 Expression of Fusions of the a-Factor Leader with
Proteins Foreign to Yeast 271
14 3 Modifications of the a-Factor Secretion System 273
14 4 Conclusions 277
References 278
15 Use of a cDNA Expression-Cloning Vector and a Secretion
Vector for Mammalian Gene Expression in
Saccharomyces cerevisiae 281
Atsushi Miyajima and Ken-ichi Aral
15 1 Development of a cDNA Expression-Cloning Vector 282
15 2 Expression of T Cell-Derived Growth Factors Using
a Yeast Secretion Vector 289
15 3 General Considerations about Use of the Secretion
Vector 300
References 302
xvi Contents
16 Synthesis and Processing of Thaumatin in Yeast 305
N Overbeeke
16 1 Biochemistry of Thaumatin 306
16 2 Molecular Genetics of Thaumatin 306
16 3 Expression of Thaumatin in Microorganisms 307
16 4 Future Prospects for Thaumatin 315
References 316
17 Recombinant Subunit Vaccines from Yeast 319
Philip J Barr, Helen L Gibson, Ian C Bathurst,
Elizabeth A Sabin, Angelica Medina-Selby, Doris Coit,
and Pablo Valenzuela
Index 341
Hepatitis B
Malaria
AIDS
Conclusions
References
|
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| indexdate | 2024-07-09T16:09:26Z |
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| series2 | Biotechnology series |
| spelling | Yeast genetic engineering ed. by Philip J. Barr ... Boston u.a. Butterworths 1989 XVIII, 354 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Biotechnology series 13 Nebent.: Yeast genetic engineering / Philip J. Barr ; Anthony J. Brake ; Pablo Valenzuela Génie génétique ram Levures (Botanique) - Biotechnologie ram Levures (Botanique) - Génie génétique Genetic Engineering Yeast fungi Genetic engineering Yeasts genetics Saccharomycetaceae (DE-588)4131614-9 gnd rswk-swf Gentechnologie (DE-588)4071722-7 gnd rswk-swf Hefeartige Pilze (DE-588)4230509-3 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Hefeartige Pilze (DE-588)4230509-3 s Gentechnologie (DE-588)4071722-7 s DE-604 Saccharomycetaceae (DE-588)4131614-9 s Barr, Philip J. Sonstige oth Biotechnology series 13 (DE-604)BV000007885 13 HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=002602099&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Yeast genetic engineering Biotechnology series Génie génétique ram Levures (Botanique) - Biotechnologie ram Levures (Botanique) - Génie génétique Genetic Engineering Yeast fungi Genetic engineering Yeasts genetics Saccharomycetaceae (DE-588)4131614-9 gnd Gentechnologie (DE-588)4071722-7 gnd Hefeartige Pilze (DE-588)4230509-3 gnd |
| subject_GND | (DE-588)4131614-9 (DE-588)4071722-7 (DE-588)4230509-3 (DE-588)4143413-4 |
| title | Yeast genetic engineering |
| title_auth | Yeast genetic engineering |
| title_exact_search | Yeast genetic engineering |
| title_full | Yeast genetic engineering ed. by Philip J. Barr ... |
| title_fullStr | Yeast genetic engineering ed. by Philip J. Barr ... |
| title_full_unstemmed | Yeast genetic engineering ed. by Philip J. Barr ... |
| title_short | Yeast genetic engineering |
| title_sort | yeast genetic engineering |
| topic | Génie génétique ram Levures (Botanique) - Biotechnologie ram Levures (Botanique) - Génie génétique Genetic Engineering Yeast fungi Genetic engineering Yeasts genetics Saccharomycetaceae (DE-588)4131614-9 gnd Gentechnologie (DE-588)4071722-7 gnd Hefeartige Pilze (DE-588)4230509-3 gnd |
| topic_facet | Génie génétique Levures (Botanique) - Biotechnologie Levures (Botanique) - Génie génétique Genetic Engineering Yeast fungi Genetic engineering Yeasts genetics Saccharomycetaceae Gentechnologie Hefeartige Pilze Aufsatzsammlung |
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