Principles of enzymatic analysis:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English German |
| Veröffentlicht: |
Weinheim u.a.
Verl. Chemie
1978
|
| Ausgabe: | 1. ed. |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVI, 260 S. Ill. u. graph. Darst. |
| ISBN: | 3527256784 0895730065 |
Internformat
MARC
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| 245 | 1 | 0 | |a Principles of enzymatic analysis |c ed. by Hans Ulrich Bergmeyer |
| 250 | |a 1. ed. | ||
| 264 | 1 | |a Weinheim u.a. |b Verl. Chemie |c 1978 | |
| 300 | |a XVI, 260 S. |b Ill. u. graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 650 | 4 | |a Analyse enzymatique | |
| 650 | 4 | |a Enzymes | |
| 650 | 4 | |a Enzymatic analysis | |
| 650 | 4 | |a Enzymes |x analysis | |
| 650 | 0 | 7 | |a Enzymatische Analyse |0 (DE-588)4014989-4 |2 gnd |9 rswk-swf |
| 689 | 0 | 0 | |a Enzymatische Analyse |0 (DE-588)4014989-4 |D s |
| 689 | 0 | |5 DE-604 | |
| 700 | 1 | |a Bergmeyer, Hans Ulrich |e Sonstige |4 oth | |
| 856 | 4 | 2 | |m SWB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=001480299&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-001480299 | ||
Datensatz im Suchindex
| _version_ | 1804116709072699392 |
|---|---|
| adam_text | IMAGE 1
CONTENTS
PREFACE V
CONTENTS VII
CONTRIBUTORS XI
ABBREVIATIONS XIII
I. INTRODUCTION
1. TERMINOLOGY, IMPORTANCE, AND LIMITS OF ENZYMATIC ANALYSIS 1
2. NOMENCLATURE AND UNITS IN ENZYMOLOGY 6
II. THEORETICAL PRINCIPLES
1. REACTION KINETICS
1.1. FUNDAMENTALS OF REACTION KINETICS 13
1.2. KINETICS OF ENZYME-CATALYZED REACTIONS 17
2. DETERMINATIONS OF MICHAELIS CONSTANTS AND INHIBITOR CONSTANTS
2.1. MICHAELIS CONSTANTS 29
2.2. INHIBITOR CONSTANTS 35
3. DETERMINATION OF SUBSTANCE CONCENTRATION OF METABOLITES (END-POINT
METHODS)
3.1. SIMPLE END-POINT METHODS 40
3.2. MEASUREMENTS WITH AID OF COUPLED REACTIONS 46
4. DETERMINATION OF THE CATALYTIC ACTIVITY OF ENZYMES
4.1. FACTORS AFFECTING CATALYTIC ACTIVITY 56
4.2. DETERMINATION OF THE OPTIMUM SUBSTANCE CONCENTRATION OF SUBSTRATES
61 4.3. DETERMINATION OF OPTIMUM CONDITIONS FOR THE AUXILIARY AND
INDICATOR REACTIONS IN COUPLED ENZYME REACTIONS 69
4.4. SOME MEASURING TECHNIQUES 76
5. DETERMINATION OF THE SUBSTANCE CONCENTRATION OF METABOLITES (KINETIC
METHODS)
5.1. FUNDAMENTALS OF REACTION KINETICS 79
5.2. KINETIC ASSAYS 81
5.3. CATALYTIC ASSAYS 83
5.4. KINETICS OF ENZYMATIC CYCLING 86
6. VISUALIZATION OF NAD(P)-DEPENDENT REACTIONS 6.1. TETRAZOLIUM SALTS
AND THEIR REDUCTION 88
6.2. APPLICATION TO ENZYMATIC REACTIONS 91
IMAGE 2
VIII CONTENTS
7. PRINCIPLES OF ENZYME-IMMUNOASSAYS
7.1. INTRODUCTION 93
7.2. HETEROGENEOUS ENZYME-IMMUNOASSAYS (EIA/ELISA) 94
7.3. HOMOGENEOUS ENZYME-IMMUNOASSAYS (EMIT) 96
7.4. CONCLUSION 97
III. HANDLING OF BIOCHEMICAL REAGENTS AND SAMPLES
1. REAGENTS FOR ENZYMATIC ANALYSIS
1.1. NOMENCLATURE, ABBREVIATIONS, AND STANDARDIZATION 99
1.2. QUALITY REQUIREMENTS 100
1.3. STORAGE, STABILITY, AND CONTROL OF SUBSTANCES AND SOLUTIONS 106
2. THE SAMPLE
2.1. SAMPLE COLLECTION 109
2.2. STABILITY OF METABOLITES AND ENZYMES IN THE SAMPLE I LL
2.3. TREATMENT OF SAMPLE FOR ENZYMATIC ANALYSIS 114
IV. MEASURING TECHNIQUES AND INSTRUMENTS
1. VOLUME MEASUREMENT
1.1. INTRODUCTION 119
1.2. PIPETTES 122
1.3. MECHANICAL DISPENSERS 132
2. ABSORPTION PHOTOMETRY
2.1. THEORETICAL BASIS AND TERMINOLOGY 135
2.2. CUVETTE HOLDERS, CUVETTES, TECHNIQUES OF MEASUREMENT, AND
EVALUATION 142 2.3. SOURCES OF ERROR AND CONTROL METHODS 146
3. OTHER MEASUREMENT TECHNIQUES
3.1. CONDUCTOMETRY 152
3.2. POTENTIOMETRY AND POLAROMETRY 155
3.3. MICROCALORIMETRY 163
3.4. MEASUREMENT TECHNIQUES WITH IMMOBILIZED ENZYMES 168
4. THE AUTOMATION OF ANALYSIS
4.1. AUTOMATIC DEVICES FOR ENZYMATIC ANALYSIS 176
4.2. WORKING PRINCIPLES OF AUTOMATIC ANALYZERS 182
4.3. FAST ANALYZERS 193
5. ENZYMATIC ANALYSIS WITH RADIOBIOCHEMICALS
5.1. GENERAL 202
5.2. DETERMINATION OF SUBSTRATES WITH RADIOBIOCHEMICALS 204
5.3. DETERMINATION OF THE CATALYTIC ACTIVITY OF ENZYMES WITH
RADIOBIOCHEMICALS 207
IMAGE 3
CONTENTS IX
V. EVALUATION AND ASSESSMENT OF EXPERIMENTAL RESULTS
1. EXPERIMENTAL DATA 213
2. EXPERIMENTAL RESULTS AND UNITS OF REFERENCE 216
3. EVALUATION AND CONTROL OF THE QUALITY OF EXPERIMENTAL RESULTS 3.1.
STATISTICAL TERMS AND METHODS 219
3.2. MATHEMATICAL EVALUATION OF EXPERIMENTS 223
3.3. STATISTICAL EVALUATION OF RESULTS 225
3.4. QUALITY CONTROL 233
4. FORMULAS 236
APPENDIX NUMBERING AND CLASSIFICATION OF ENZYMES 242
ATOMIC WEIGHTS 246
THE INTERNATIONAL SYSTEM OF UNITS (SI) 247
INDEX 253
|
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| discipline | Chemie / Pharmazie Biologie |
| edition | 1. ed. |
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| id | DE-604.BV002252744 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T15:42:49Z |
| institution | BVB |
| isbn | 3527256784 0895730065 |
| language | English German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-001480299 |
| oclc_num | 4002464 |
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| owner_facet | DE-20 DE-29 DE-11 DE-188 |
| physical | XVI, 260 S. Ill. u. graph. Darst. |
| publishDate | 1978 |
| publishDateSearch | 1978 |
| publishDateSort | 1978 |
| publisher | Verl. Chemie |
| record_format | marc |
| spelling | Principles of enzymatic analysis ed. by Hans Ulrich Bergmeyer 1. ed. Weinheim u.a. Verl. Chemie 1978 XVI, 260 S. Ill. u. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Analyse enzymatique Enzymes Enzymatic analysis Enzymes analysis Enzymatische Analyse (DE-588)4014989-4 gnd rswk-swf Enzymatische Analyse (DE-588)4014989-4 s DE-604 Bergmeyer, Hans Ulrich Sonstige oth SWB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=001480299&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Principles of enzymatic analysis Analyse enzymatique Enzymes Enzymatic analysis Enzymes analysis Enzymatische Analyse (DE-588)4014989-4 gnd |
| subject_GND | (DE-588)4014989-4 |
| title | Principles of enzymatic analysis |
| title_auth | Principles of enzymatic analysis |
| title_exact_search | Principles of enzymatic analysis |
| title_full | Principles of enzymatic analysis ed. by Hans Ulrich Bergmeyer |
| title_fullStr | Principles of enzymatic analysis ed. by Hans Ulrich Bergmeyer |
| title_full_unstemmed | Principles of enzymatic analysis ed. by Hans Ulrich Bergmeyer |
| title_short | Principles of enzymatic analysis |
| title_sort | principles of enzymatic analysis |
| topic | Analyse enzymatique Enzymes Enzymatic analysis Enzymes analysis Enzymatische Analyse (DE-588)4014989-4 gnd |
| topic_facet | Analyse enzymatique Enzymes Enzymatic analysis Enzymes analysis Enzymatische Analyse |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=001480299&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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