Zone electrophoresis in blocks and columns:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Amsterdam [u.a.]
Elsevier
1963
|
| Schriftenreihe: | Elsevier monographs
28 : Chemistry section |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | VIII, 219 S. Ill., graph. Darst. |
Internformat
MARC
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| 245 | 1 | 0 | |a Zone electrophoresis in blocks and columns |c H. Bloemendal |
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| 300 | |a VIII, 219 S. |b Ill., graph. Darst. | ||
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| 490 | 1 | |a Elsevier monographs |v 28 : Chemistry section | |
| 650 | 4 | |a Électrophorèse de zone | |
| 650 | 4 | |a Electrophoresis | |
| 650 | 4 | |a Zone electrophoresis | |
| 830 | 0 | |a Elsevier monographs |v 28 : Chemistry section |w (DE-604)BV000900457 |9 28 | |
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Datensatz im Suchindex
| _version_ | 1804116685055066112 |
|---|---|
| adam_text | ZONE
ELECTROPHORESIS
IN
BLOCKS AND COLUMNS
BY
I-I BLOEMENDAL
Department of Biochemistry,
Antoni van Leemvenboekhitis, The Netherlands Cancer Institute,
Amsterdam (The Netherlands)
ELSEVIER PUBLISHING COMPANY
AMSTERDAM-LONDON-NEWYORK
CONTENTS
Chapter 1 INTRODUCTION
Chapter 2 BLOCK ELECTROPHORESIS
1 Apparatus
a Power supply
b Troughs
2 Buffers
3 Preparation of the supporting medium
a Starch (granules)
b Polyvinyl chloride
c Sponge rubber and foam plastic
d Glass (powder, beads)
e Hyflo Super Cel; Celite
f Sephadex
4 Electrode vessels
5 Safety system
6 Insertion of the sample
7 Electrophoretic run
8 Influence of electro-osmosis
9 Influence of temperature
10 Location of substance
11 Cutting
12 Elution
13 Estimations
14 Applications
a Starch block electrophoresis
(i) Proteins, peptides, protein hormones, enzymes
(ii) Nucleoproteins and nucleic acids
(iii) Homogeneity studies
b Polyvinyl chloride block electrophoresis
VICONTENTS
c Sponge block electrophoresis 52
d Glass block electrophoresis 52
e Hyflo Super Cel and Celite block electrophoresis 53
f Sephadex block electrophoresis 53
g Asbestos block electrophoresis 54
Chapter 3 GEL ELECTROPHORESIS 55
1 Apparatus 56
2 Electrodes 60
3 Preparation of the supporting medium 61
a Starch 61
b Strips for micro starch gel method 63
c Silica gel 64
d Polyacrylamide gel 64
4 Insertion of the sample 66
aa Starch gel (macro method) 66
ab Starch gel (micro method) 70
b Silica gel 70
c Polyacrylamide gel 70
5 Electrophoretic run 71
6 Influence ol temperature 71
7 Location of substance 73
8 Elution from starch gel 78
9 Quantitative evaluation of starch gel strips 81
10 Photography of stained gels 86
11 Applications 87
a Starch gel electrophoresis 87
(i) Serum proteins 87
(ii) Other proteins, enzymes and hormones 93
(iii) Non-protein substances 96
b Silica gel electrophoresis 97
c Polyacrvlamide gel 99
Chapter 4 CONTINUOUS ELECTROPHORESIS 102
1 Apparatus 104
2 Electrodes 105
CONTENTSVII
3 Regulation of buffer flow 107
4 Automatic regulation of bath current 108
a The regulating equipment 110
b The transductor amplifier 113
c The pump motor 114
5 Preparation of packing material 115
a Glass 115
b Other supporting media 115
6 Electrophoretic run 115
7 Applications 118
Chapter 5 COLUMN ELECTROPHORESIS 120
1 Apparatus 120
2 Electrodes 132
3 Preparation of the supporting medium 132
a Cellulose 132
b Polyvinyl chloride resin 133
c Starch 133
d Glass 134
4 Introduction of the sample 134
5 Electrophoretic run 134
6 Influence of temperature 135
7 Influence of electro-osmosis 144
8 Elution and automatic analysis 144
9 Application 149
a Cellulose column electrophoresis 149
Proteins, amino acids, hormones, enzymes, nucleic
acids, mononucleotides 149
b Starch column electrophoresis 153
Proteins, enzymes, thiamine and vitamin B6
compounds 153
c Polyvinyl chloride column electrophoresis 155
d Glass column electrophoresis 157
e Alumina column electrophoresis 157
f Sephadex column electrophoresis 157
Counter-current electrophoresis 157
viir CONTENTS
Chapter 6 COLUMN ELECTROPHORESIS IN
DENSITYGRADIENTS159
1 The density gradient 160
2 Apparatus 163
3 Medium and buffers 164
4 Filling, according to Svensson et al 165
5 Insertion of the sample 166
6 Collecting of fractions 166
7 Modified procedures 166
8 Filling procedure after Colehour 167
9 Ethanol gradients 168
10 The improved procedure of Svensson and Valmet 168
11 The method of Berg and Beeler 169
12 The method of Kolin 171
13 The apparatus of Choules and Ballentine 173
14 Filling procedure 186
15 Cutting • 178
16 Electrophoresis 178
17 Applications 178
a Svensson s method 178
b Kolin s method 181
c Other methods 183
Chapter 7 CONCLUDING REMARKS 184
References 187
Author index 201
Subject index 215
|
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| discipline | Chemie / Pharmazie |
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| id | DE-604.BV002229995 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T15:42:26Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-001465501 |
| oclc_num | 1512421 |
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| physical | VIII, 219 S. Ill., graph. Darst. |
| psigel | HUB-ZB011200709 |
| publishDate | 1963 |
| publishDateSearch | 1963 |
| publishDateSort | 1963 |
| publisher | Elsevier |
| record_format | marc |
| series | Elsevier monographs |
| series2 | Elsevier monographs |
| spelling | Bloemendal, Hans 1923-2015 Verfasser (DE-588)120709406 aut Zone electrophoresis in blocks and columns H. Bloemendal Amsterdam [u.a.] Elsevier 1963 VIII, 219 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Elsevier monographs 28 : Chemistry section Électrophorèse de zone Electrophoresis Zone electrophoresis Elsevier monographs 28 : Chemistry section (DE-604)BV000900457 28 HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=001465501&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Bloemendal, Hans 1923-2015 Zone electrophoresis in blocks and columns Elsevier monographs Électrophorèse de zone Electrophoresis Zone electrophoresis |
| title | Zone electrophoresis in blocks and columns |
| title_auth | Zone electrophoresis in blocks and columns |
| title_exact_search | Zone electrophoresis in blocks and columns |
| title_full | Zone electrophoresis in blocks and columns H. Bloemendal |
| title_fullStr | Zone electrophoresis in blocks and columns H. Bloemendal |
| title_full_unstemmed | Zone electrophoresis in blocks and columns H. Bloemendal |
| title_short | Zone electrophoresis in blocks and columns |
| title_sort | zone electrophoresis in blocks and columns |
| topic | Électrophorèse de zone Electrophoresis Zone electrophoresis |
| topic_facet | Électrophorèse de zone Electrophoresis Zone electrophoresis |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=001465501&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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