Cross electrophoresis: its principles and applications
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Tokyo u.a.
Igaku Shoin u.a.
1966
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | IX, 194 S. |
Internformat
MARC
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| 245 | 1 | 0 | |a Cross electrophoresis |b its principles and applications |
| 264 | 1 | |a Tokyo u.a. |b Igaku Shoin u.a. |c 1966 | |
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| 650 | 4 | |a Protéines | |
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| 650 | 4 | |a Électrophorèse sur papier | |
| 650 | 4 | |a Electrophoresis | |
| 650 | 4 | |a Proteins | |
| 650 | 4 | |a Proteins |x Analysis | |
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Datensatz im Suchindex
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|---|---|
| adam_text | CROSS ELECTROPHORESIS
Its Principle and Applications
by
SHOJI R O NAKAM URA
IGAKU SHOIN Ltd
Tokyo
ELSEVIER PUBLISHING COMPANY
Amsterdam - London - New York
CONTENTS
Preface v
Chapter I Principle of Cross Electrophoresis
1-1 Introduction 1
1-2 Crossing by the continuous paper electrophoresis 1
1*3 Cross paper electrophoresis 3
Chapter 2 Apparatus and Procedure
2-1 One-dimensional cross paper electrophoresis 6
2-1-1 Procedure of one-dimensional cross paper electrophoresis 6
2-1-2 Artifact appearing at the crossing point on the line of a
substance 7
2-1-3 Precautions to prevent artifacts 16
2-2 Two-dimensional cross paper electrophoresis 19
2-2-1 Principle of the two-dimensional cross electrophoresis 19
2-2-2 Apparatus for two-dimensional techniques 20
2-2-3 Designs for two-dimensional cross electrophoresis 22
? 2-2-4 Experimental procedures of t o-dimensional technique 23
2-2-5 Cross diagram 27
2-3 Quantitative measurements by cross electrophoresis 28
2-3-1 Method to obtain a concentration distribution curve from
the cross diagram (Theory of cross diagram) 28
2-3-2 Relationship between the area under the cross diagram
and the quantity of reacting material 34
2-3-3 Measurement of reactant bv overrunning technique 36
Chapter 3 Applications to Imniunocheniistrv
3-1 Reaction of serum with its homologous antiserum by cross
electrophoresis 39
3-1-1 Reaction by one-dimennonal technique 39
3-1-2 Reaction of human serum and its homologous antiserum
by two-dimensional technique 40
3-1-3 Comparison with Immunoelectrophoresis 41
3-2 Reactions of diphtheria toxoid with antiserum 42
3-2-1 Electrophoredc patterns of diphtheria toxoid and antiserum 42
3-2-2 Reaction of diphtheria toxoid with antiserum by one-
dimensional cross electrophoresis 42
3-2-3 Reaction of diphtheria toxoid with antiserum by two-
dimensional cross electrophoresis 43
v i i
3-2-4 The antibody which corresponds to the peak in /3-globulin
zone of the cross diagram of the toxoid 46
3-2-5 Antibodies corresponding to the flat peaks of the cross
diagram of the toxoid 48
3-2-6 Reactions of purified antiserum and toxoid 50
3-3 Reaction of snake venom with its antiserum 52
3-3-1 Electrophoretic patterns of viper enom and its antiserum 52
3-3-2 One-dimensional cross electrophoresis of the venom of the
Japanese viper with its antiserum 54
3-3-3 Two-dimensional cross electrophoresis of viper venom and its
antiserum 55
3-3-4 Distribution of antigens in the Japanese viper venom 58
3-4 Other applications of cross electrophoresis to the immunochemical
reactions 59
3-4-1 Quantitative applications 59
3-4-2 Detection of incomplete antibody 62
3-4-3 Demonstration of the cross reactions 63
Chapter 4 Reactions of a Specific Protein of Legumes with Various Proteins
4-1 Reaction of concanavalin A with -era of various animals 66
4-1-1 A specific protein of jack bean 66
4-1-2 Identification of protein J with concanavalin A 70
4-1-3 Cross diagrams of sera of various animals against
3 concanavalin A 74
4-1-4 Relationship between protein fractions ol sera ol various
animals 84
4-2 Reactions of concanvalin A with gg proteins 87
4-2-1 Proteins of egg white 87
4-2-2 Cross diagrams of egg whites of various birds against
concanavalin A 88
4-2-3 Cross diagrams of egg yolk : ( bird against concanavalin A 89
4-3 Reactions of concanavalin A with other proteins 91
4-3-1 Reaction of concanavalin A with milk protein 91
4:3-2 Reactions of concanavalin A with the protein of beans 93
4-3-3 Reactions of concanavalin A with the proteins ol viper
venom 93
4-4 Specific proteins of other beans 93
Chapter 5 Observations of Serum Protein Fractions by Means of Cross
Diagrams
5-1 Possibility of observing the changes ot serum protein fractions by
means of cross diagrams of serum against various substances 95
5-2 Cross diagrams of sera in disease-, against concanavalin A 95
5-2-1 Changes of serum protein-- and that ot cross diagrams 95
5-2-2 Classification of the changed cross diagrams 100
Vlll
5-2-3 Characteristics of cross diagrams in diseases 105
5-2-4 Relationship between the change of cross diagram and thai
ol the concentrations oi scrum proteins 112
5-3 Cross diagrams of sera against hemoglobin 114
5-4 Interactions of various organic substances with serum and tissue
proteins 116
5-4-1 Interaction of serum proteins with drugs and related
substances 116
5-4-2 Interaction of curare and soluble proteins of various tissues 119
5-4-3 Reactions of biological]v interesting substances observed
by cross electrophoresis 120
Chapter 6 Distribution of Trypsin Inhibitors
6-1 Trypsin inhibitors 121
6-2 Distribution of trypsin inhibitors in the serum proteins 121
6-2-1 Investigations of Jacobs-on 121
6-2-2 Investigations of trypsin inhibitors in serum by cross paper
electrophoresis 122
6-2-3 Cross diagrams of sera of various animals against trypsin 127
6-3 Distribution of trypsin inhibitors in egg white proteins of various
birds 136
6-3-1 Trypsin inhibitors in chicken egg white 135
6-3-2 Trypsin inhibitors in egg whiles of various other birds 138
6-3-3 Trvpsin inhibitors of leguminous seeds 147 9 -i
Chapter 7 Proof of the Formation of Enzyme-Substrate Complex
7-1 Mechanism of enzyme action and the formation of enzvme-
substrate complex 151
7-2 Proof of the formation of enzyme-substrate complex by means of
cross electrophoresis 152
7-2-1 Possibility of proving the complex formation by means of
cross electrophoresis 152
7-2-2 Examples of the proof of the formation of enzyme-
• substrate complex by ctoss electrophoresis 153
7-2-3 Conclusion 163
7-3 Complex formation of inactive enzymes with substrate 164
7-3-1 Activity of enzyme and complex formation 164
7-3-2 Proof of the formation of the complex of inactive enzyme
and substrate by cross electrophoresis 164
7-3-3 Binding site and activitv site on the enzyme molecule 170
References 173
Author Index 183
Subject Index 190
|
| any_adam_object | 1 |
| author | Nakamura, Shojiro |
| author_facet | Nakamura, Shojiro |
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| discipline | Chemie / Pharmazie |
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| indexdate | 2024-07-09T15:39:31Z |
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| language | English |
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| physical | IX, 194 S. |
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| publishDate | 1966 |
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| spelling | Nakamura, Shojiro Verfasser aut Cross electrophoresis its principles and applications Tokyo u.a. Igaku Shoin u.a. 1966 IX, 194 S. txt rdacontent n rdamedia nc rdacarrier Elektroforese gtt Protéines Électrophorèse Électrophorèse sur papier Electrophoresis Proteins Proteins Analysis HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=001341879&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Nakamura, Shojiro Cross electrophoresis its principles and applications Elektroforese gtt Protéines Électrophorèse Électrophorèse sur papier Electrophoresis Proteins Proteins Analysis |
| title | Cross electrophoresis its principles and applications |
| title_auth | Cross electrophoresis its principles and applications |
| title_exact_search | Cross electrophoresis its principles and applications |
| title_full | Cross electrophoresis its principles and applications |
| title_fullStr | Cross electrophoresis its principles and applications |
| title_full_unstemmed | Cross electrophoresis its principles and applications |
| title_short | Cross electrophoresis |
| title_sort | cross electrophoresis its principles and applications |
| title_sub | its principles and applications |
| topic | Elektroforese gtt Protéines Électrophorèse Électrophorèse sur papier Electrophoresis Proteins Proteins Analysis |
| topic_facet | Elektroforese Protéines Électrophorèse Électrophorèse sur papier Electrophoresis Proteins Proteins Analysis |
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