Microscope photometry:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Berlin [u.a.]
Springer
1977
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | X, 253 S. Ill., graph. Darst. |
| ISBN: | 3540080945 0387080945 |
Internformat
MARC
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| 100 | 1 | |a Piller, Horst |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Microscope photometry |c Horst Piller |
| 264 | 1 | |a Berlin [u.a.] |b Springer |c 1977 | |
| 300 | |a X, 253 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 650 | 7 | |a Fotometrie |2 gtt | |
| 650 | 7 | |a Microscopen |2 gtt | |
| 650 | 7 | |a Optische verschijnselen |2 gtt | |
| 650 | 7 | |a physics |2 cabt | |
| 650 | 7 | |a microscopy |2 cabt | |
| 650 | 7 | |a analytical methods |2 cabt | |
| 650 | 7 | |a wavelengths |2 cabt | |
| 650 | 7 | |a radiation |2 cabt | |
| 650 | 7 | |a optical instruments |2 cabt | |
| 650 | 4 | |a Microphotometry | |
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| 650 | 0 | 7 | |a Mikroskopie |0 (DE-588)4039238-7 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Fotometrie |0 (DE-588)4174524-3 |2 gnd |9 rswk-swf |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-000742383 | ||
Datensatz im Suchindex
| _version_ | 1804115626689560576 |
|---|---|
| adam_text | Contents
Chapter 1 General Introduction............ 1
Chapter 2 Ray Paths in the Microscope........ 4
2.1 Introduction............. 4
2.2 Illumination Systems for Bright Field . . 10
2.3 Imaging Systems for Bright Field .... 12
2.4 Dark-Field Systems.......... 13
2.5 Diaphragms............. 14
2.6 Instrumental Parameters........ 15
2.7 Definition of Geometrical Optical Terms
in Alphabetical Order......... 17
Chapter 3 Transfer of Light Energy in the Optical System 22
3.1 Introduction............. 22
3.2 Spectral Concentration of Radiance of the
Lamp................ 24
3.3 Spectral Bandwidth.......... 24
3.4 Optical Flux............. 25
3.5 Transmittance of the Optical System ... 33
3.6 Interaction of Light with the Stage Object 35
3.7 Relation Between Light Power and Photo-
metric Measuring Values........ 36
3.8 Sensation of Brightness in the Observer 37
Chapter 4 The Light Source and Its Power Supply .... 41
4.1 Introduction............. 41
4.2 Stabilised Electric Power Supply..... 41
4.3 Various Types of Lamps........ 43
4.4 The Collector............. 48
4.5 Light Modulators........... 49
Chapter 5 Monochromatising Devices and Filters..... 51
5.1 Introduction............. 51
5.2 Characteristics of Monochromatising De-
vices ................ 52
5.3 Monochromatic Interference Filters ... 57
5.4 Prism- and Grating-Monochromators . . 64
5.5 Filters Other Than Monochromatising . . 72
5.6 Polarising Devices........... 73
VIII Contents
Chapter 6 The Basic Microscope Assembly....... 78
6.1 Condensers.............. 78
6.2 Reflectors for Reflected-Light Work ... 80
6.3 Types of Microscope Stage....... 85
6.4 Objectives.............. 86
6.5 Oculars............... 94
6.6 Body Tubes and Tube Optics...... 97
Chapter 7 The Photometer System........... 99
7.1 Photometer Heads.......... 99
7.2 Photomultiplier Tubes......... 101
7.3 Electrical Conversion Modules..... 107
Chapter 8 Adjustment of Equipment.......... 112
8.1 Microscope Axis and Stage-Rotation Axis 112
8.2 Luminous-Field Diaphragm...... 112
8.3 Condenser-Aperture Diaphragm and the
Luminous Surface of Lamps...... 113
8.4 Levelling of the Stage Object in Reflected
Light................ 116
8.5 Crossing the Polars and Orienting the
Cross-Wires............. 117
Chapter 9 Procedures in Photometry with the Microscope 120
9.1 Principle of Measurements....... 120
9.2 Photometer Systems.......... 121
9.3 Reference Materials.......... 125
9.4 Measuring Procedures......... 128
9.5 Computation of Measuring Results . . .131
9.6 Automation in Measuring and Data Pro-
cessing ............... 138
Chapter 10 Special Techniques in Microscope Photometry . . 141
10.1 Microscope Fluorometry........141
10.2 Photometric Determination of Optical-Path
Differences..............143
10.3 Diffuse Reflection from Surfaces .... 149
10.4 Techniques for Measuring Micro-Auto-
radiographs.............150
10.5 Micro-Densitometry..........154
10.6 Determination of Basic Optical Constants
with Interference-Layer Techniques . . .157
Chapter 11 Special Adjustment for Photometry...... 161
11.1 Focusing of Objective......... 161
11.2 Levelling of Stage Object in Specularly
Reflected Light............ 164
Contents IX
11.3 Control of Photometric Field and Luminous
Field................165
11.4 Control of Angle of Incidence.....167
11.5 Control of Spectral Bandwidth.....170
11.6 Setting of Central Wavelength.....171
Chapter 12 Systematic Measuring Errors.........172
12.1 Distinction Between Systematic and Sta-
tistical Errors.............172
12.2 General Aspects of Systematic Errors . . 173
12.3 Instrumental Factors Affecting the Light
Supply to the Photosensor.......174
12.4 Stage-Object Factors Affecting the Light
Supply to the Photosensor.......185
Chapter 13 Statistical Errors..............189
13.1 Causes of Statistical Errors.......189
13.2 Expressions and Terms in Error Statistics 189
13.3 Evaluation of Precision with a Limited
Number of Tests...........190
13.4 Comparison of Precision in Different Tests 191
13.5 Summation of Standard Deviations . . . 192
Chapter 14 Application of Microscope Photometry..... 194
14.1 Absorption Parameters Measured in Trans-
mitted Light............. 194
14.2 Parameters Measured in Reflected Light 197
14.3 Microscope Fluorometry........ 199
14.4 Refraction Parameters Derived from Opti-
cal-Path Difference.......... 200
Appendix 1. Specification of a Light Wave.......201
2. Quantities, Units and Symbols......202
3. Accumulation of Errors.........213
4. Detectivity and the Effect of Statistical Spread 214
5. Systematic Errors Due to Increments in
Measuring Values............217
6. Experimental Determination of Factors In-
volved in the Spectral Sensitivity of Measuring
Equipment...............220
7. Correlation Between Photometric Values and
Phase Differences in Double-Beam Inter-
ference Microscopy...........224
8. Theory of Interference-Layer Technique . . 227
9. Determination of Relative Difference in Re-
fractive Index of Two Contiguous Media . . 230
10. Theory of Secondary Effect of Glare ... 230
Contents
11. Mathematical Derivation of the Distributional
Error.................233
12. Beam Splitting in an Inclined Glass Plate and
the Effect of Parasitic Images.......235
13. Derivation of the cos4-Law........238
14. Determination of Angles Between the Micro-
scope Axis and Rays Deflected at, or Tra-
versing, the Stage Object.........239
References......................244
Subject Index....................251
|
| any_adam_object | 1 |
| author | Piller, Horst |
| author_facet | Piller, Horst |
| author_role | aut |
| author_sort | Piller, Horst |
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| building | Verbundindex |
| bvnumber | BV001233246 |
| callnumber-first | Q - Science |
| callnumber-label | QC391 |
| callnumber-raw | QC391 |
| callnumber-search | QC391 |
| callnumber-sort | QC 3391 |
| callnumber-subject | QC - Physics |
| classification_rvk | UH 6700 UH 6900 WC 2900 |
| classification_tum | CHE 264f |
| ctrlnum | (OCoLC)2967943 (DE-599)BVBBV001233246 |
| dewey-full | 578/.4 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 578 - Natural history of organisms |
| dewey-raw | 578/.4 |
| dewey-search | 578/.4 |
| dewey-sort | 3578 14 |
| dewey-tens | 570 - Biology |
| discipline | Physik Biologie Chemie |
| format | Book |
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| id | DE-604.BV001233246 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T15:25:36Z |
| institution | BVB |
| isbn | 3540080945 0387080945 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-000742383 |
| oclc_num | 2967943 |
| open_access_boolean | |
| owner | DE-19 DE-BY-UBM DE-91 DE-BY-TUM DE-355 DE-BY-UBR DE-20 DE-83 DE-11 DE-188 |
| owner_facet | DE-19 DE-BY-UBM DE-91 DE-BY-TUM DE-355 DE-BY-UBR DE-20 DE-83 DE-11 DE-188 |
| physical | X, 253 S. Ill., graph. Darst. |
| publishDate | 1977 |
| publishDateSearch | 1977 |
| publishDateSort | 1977 |
| publisher | Springer |
| record_format | marc |
| spelling | Piller, Horst Verfasser aut Microscope photometry Horst Piller Berlin [u.a.] Springer 1977 X, 253 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Fotometrie gtt Microscopen gtt Optische verschijnselen gtt physics cabt microscopy cabt analytical methods cabt wavelengths cabt radiation cabt optical instruments cabt Microphotometry Mikrofotometrie (DE-588)4169839-3 gnd rswk-swf Mikroskop (DE-588)4039237-5 gnd rswk-swf Mikroskopie (DE-588)4039238-7 gnd rswk-swf Fotometrie (DE-588)4174524-3 gnd rswk-swf Mikroskop (DE-588)4039237-5 s Fotometrie (DE-588)4174524-3 s DE-604 Mikrofotometrie (DE-588)4169839-3 s Mikroskopie (DE-588)4039238-7 s HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=000742383&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Piller, Horst Microscope photometry Fotometrie gtt Microscopen gtt Optische verschijnselen gtt physics cabt microscopy cabt analytical methods cabt wavelengths cabt radiation cabt optical instruments cabt Microphotometry Mikrofotometrie (DE-588)4169839-3 gnd Mikroskop (DE-588)4039237-5 gnd Mikroskopie (DE-588)4039238-7 gnd Fotometrie (DE-588)4174524-3 gnd |
| subject_GND | (DE-588)4169839-3 (DE-588)4039237-5 (DE-588)4039238-7 (DE-588)4174524-3 |
| title | Microscope photometry |
| title_auth | Microscope photometry |
| title_exact_search | Microscope photometry |
| title_full | Microscope photometry Horst Piller |
| title_fullStr | Microscope photometry Horst Piller |
| title_full_unstemmed | Microscope photometry Horst Piller |
| title_short | Microscope photometry |
| title_sort | microscope photometry |
| topic | Fotometrie gtt Microscopen gtt Optische verschijnselen gtt physics cabt microscopy cabt analytical methods cabt wavelengths cabt radiation cabt optical instruments cabt Microphotometry Mikrofotometrie (DE-588)4169839-3 gnd Mikroskop (DE-588)4039237-5 gnd Mikroskopie (DE-588)4039238-7 gnd Fotometrie (DE-588)4174524-3 gnd |
| topic_facet | Fotometrie Microscopen Optische verschijnselen physics microscopy analytical methods wavelengths radiation optical instruments Microphotometry Mikrofotometrie Mikroskop Mikroskopie |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=000742383&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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