Monoclonal antibodies: principles and practice ; production and application of monoclonal antibodies in cell biology, biochemistry and immunology
Saved in:
| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
London [u.a.]
Acad. Press
1986
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| Edition: | 2. ed. |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Literaturangaben |
| Physical Description: | XIV, 315 S. Ill., graph. Darst. |
| ISBN: | 0122870212 |
Staff View
MARC
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| 100 | 1 | |a Goding, James W. |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Monoclonal antibodies |b principles and practice ; production and application of monoclonal antibodies in cell biology, biochemistry and immunology |c James W. Goding |
| 250 | |a 2. ed. | ||
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| 650 | 4 | |a Antibodies, Monoclonal | |
| 650 | 4 | |a Monoclonal antibodies | |
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Record in the Search Index
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| adam_text | Monoclonal Antibodies:
Principles and Practice
Production and Application of
Monoclonal Antibodies in Cell Biology,
Biochemistry and Immunology
Second Edition
JAMES WjGODING
Department oi Pathology and Immunology,
Monash University, Alfred Hospital,
Prahran, Victoria, Australia
ACADEMIC PRESS
Harcourt Brace Jovanovich, Publishers
London Orlando
New York San Diego Austin
Boston Tokyo Sydney Toronto
Contents
Preface v
Preface to Second Edition vii
List of Abbreviations xv
1 Introduction 1
2 Theory of Monoclonal Antibodies 5
2 1 The Clonal Selection Theory • • 5
2 2 Structure of Antibodies 6
2 3 Properties of Individual Immunoglobulin Classes 11
2 4 Biosynthesis and Assembly of Immunoglobulins 21
2 5 Antibody Genes 22
2 6 Control of the Immune Response 28
2 7 Myeloma 33
2 8 Cell Fusion and Selection of Hybrids 35
2 9 Hybridomas 37
2 10 Differences Between Conventional and Monoclonal
Serology 42
3 Product ion of Monoc lona l Ant ibodies 59
3 1 Choice of Normal Lymphocyte Donor 59
3 2 Immunization Protocol 61
3 3 Choice of Myeloma Cell Line for Fusion 64
3 4 Equipment Required for Fusion and Growth of Hybridomas 66
Contents
3 5 Preparation of Spleen Cells 67
3 6 Preparation of Myeloma Cells 68
3 7 Preparation of HAT and HT Medium 70
3 8 Fusion Protocol 71
3 9 Early Growth 74
3 10 Screening Assays 76
3 11 Cloning 89
3 12 Large-scale Cultures 91
3 13 Storage of Hybrids in Liquid Nitrogen 91
3 14 Growth of Hybridomas in Animals 92
3 15 Mix-ups of Cells 93
3 16 Mycoplasma Contamination 93
Purification, Fragmentation and Isotopic Labelling
of Monoclonal Antibodies 104
4 1 Determination of Antibody Class 105
4 2 Methods Used in Antibody Purification 108
4 3 Purification of Monoclonal IgG 121
4 4 Purification of IgM 125
4 5 Fragmentation of Monoclonal Antibodies 125
4 6 Radiolabelling of Monoclonal Antibodies 133
4 7 Conditions for Stability and Storage of Monoclonal Antibodies 135
Analysis of Antigens Recognized by Monoclonal
Antibodies 142
5 1 Determination of the Biochemical Nature of the Antigen 142
5 2 Cellular Distribution of Antigens Detected by Monoclonal
Antibodies 145
5 3 Radioiodination of Soluble Protein Antigens 145
5 4 Radiolabelling of Soluble Proteins with Tritium by Reductive
Methylation 153
5 5 Radiolabelling of Cellular Proteins 154
5 6 Solubilization of Membrane Proteins 161
5 7 Isolation of Radiolabelled Antigens by Immunoprecipitation 165
5 8 Electrophoretic Methods for Analysis of Protein Antigens 171
5 9 Topographical Analysis of Proteins by Monoclonal
Antibodies 199
5 10 Use of Antibodies in the Production and Identification of
Cloned DNA Sequences 200
Affinity Chromatography Using Monoclonal Antibodies 219
6 1 Preparation of Immobilized Antibodies 220
6 2 Use of Antibody Affinity Columns 227
Contents xi
7 Immunofluorescence 241
7 1 Principles of Immunofluorescence 242
7 2 The Fluorescence-activated Cell Sorter 252
7 3 Conjugation of Antibodies with Fluorochromes 255
7 4 The Avidin-Biotin System 262
7 5 Staining Cells with Fluorescent Antibodies 266
7 6 Nonspecific Fluorescence 271
7 7 Lack of Staining 274
8 Generation of Conventional Antibodies 281
8 1 Strategies for the Preparation of Highly Specific Polyclonal
Antibodies 282
8 2 Preparative SDS-polyacrylamide Gel Electrophoresis
8 3 Immunization 286
8 4 Purification and Fragmentation of Rabbit, Sheep and Goat
IgG 288
8 5 Specific Antibodies from Nonspecific Antisera 290
Glossary 295
Index 301
|
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| id | DE-604.BV000977209 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T15:22:03Z |
| institution | BVB |
| isbn | 0122870212 |
| language | English |
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| oclc_num | 15795770 |
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| physical | XIV, 315 S. Ill., graph. Darst. |
| publishDate | 1986 |
| publishDateSearch | 1986 |
| publishDateSort | 1986 |
| publisher | Acad. Press |
| record_format | marc |
| spelling | Goding, James W. Verfasser aut Monoclonal antibodies principles and practice ; production and application of monoclonal antibodies in cell biology, biochemistry and immunology James W. Goding 2. ed. London [u.a.] Acad. Press 1986 XIV, 315 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Literaturangaben Anticorpo E Imuneglobulina larpcal Anticorps monoclonal Anticorps monoclonaux ram Biochimie Monoklonale antistoffen gtt immunologie Antibodies, Monoclonal Monoclonal antibodies Monoklonaler Antikörper (DE-588)4040094-3 gnd rswk-swf Monoklonaler Antikörper (DE-588)4040094-3 s DE-604 HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=000593176&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Goding, James W. Monoclonal antibodies principles and practice ; production and application of monoclonal antibodies in cell biology, biochemistry and immunology Anticorpo E Imuneglobulina larpcal Anticorps monoclonal Anticorps monoclonaux ram Biochimie Monoklonale antistoffen gtt immunologie Antibodies, Monoclonal Monoclonal antibodies Monoklonaler Antikörper (DE-588)4040094-3 gnd |
| subject_GND | (DE-588)4040094-3 |
| title | Monoclonal antibodies principles and practice ; production and application of monoclonal antibodies in cell biology, biochemistry and immunology |
| title_auth | Monoclonal antibodies principles and practice ; production and application of monoclonal antibodies in cell biology, biochemistry and immunology |
| title_exact_search | Monoclonal antibodies principles and practice ; production and application of monoclonal antibodies in cell biology, biochemistry and immunology |
| title_full | Monoclonal antibodies principles and practice ; production and application of monoclonal antibodies in cell biology, biochemistry and immunology James W. Goding |
| title_fullStr | Monoclonal antibodies principles and practice ; production and application of monoclonal antibodies in cell biology, biochemistry and immunology James W. Goding |
| title_full_unstemmed | Monoclonal antibodies principles and practice ; production and application of monoclonal antibodies in cell biology, biochemistry and immunology James W. Goding |
| title_short | Monoclonal antibodies |
| title_sort | monoclonal antibodies principles and practice production and application of monoclonal antibodies in cell biology biochemistry and immunology |
| title_sub | principles and practice ; production and application of monoclonal antibodies in cell biology, biochemistry and immunology |
| topic | Anticorpo E Imuneglobulina larpcal Anticorps monoclonal Anticorps monoclonaux ram Biochimie Monoklonale antistoffen gtt immunologie Antibodies, Monoclonal Monoclonal antibodies Monoklonaler Antikörper (DE-588)4040094-3 gnd |
| topic_facet | Anticorpo E Imuneglobulina Anticorps monoclonal Anticorps monoclonaux Biochimie Monoklonale antistoffen immunologie Antibodies, Monoclonal Monoclonal antibodies Monoklonaler Antikörper |
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