Phycobiliproteins:
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Boca Raton, Fla.
CRC Pr.
1987
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 218 S. Ill. |
| ISBN: | 0849355257 |
Internformat
MARC
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Datensatz im Suchindex
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|---|---|
| adam_text | Phycobiliproteins
Authors
Robert MacColl
and
Deborah Guard-Friar
Wadsworth Center for Laboratories and Research
New York State Department of Health
Albany, New York
CRC Press, Inc
Boca Raton, Florida
TABLE OF CONTENTS
Chapter 1
Introduction 1
References 6
Chapter 2
Phycobilisomes: A Structure-Function Model 9
I Discovery 9
II Two Main Types of Phycobilisomes 10
III Isolation of Phycobilisomes 11
IV Phycobilisome Energy Transfer 14
V A Structure-Function Relationship 16
VI Electron Microscopy of Isolated Disks, Stacks, and Phycobilisomes 17
VII A Model 21
References 23
Chapter 3
Biliproteins: Covalent Structures 25
I Introduction 25
II Subunit Structure 25
III Chromophore Content 25
IV Bilin Structure 28
A Bilin Cleavage from Apoprotein 28
B Structure of Phycocyanobilin 29
C Structure of Phycoerythrobilin 31
D Cryptoviolin, Phycourobilin, and the 697-nm Bilin 32
V Chromophore-Apoprotein Linkage 33
A An Overview 33
B Phycocyanobilin Linkage to Cysteine 33
C Phycoerythrobilin Linkage to Cysteine 34
D Thioether Bond 34
E Second Covalent Linkage 35
VI Primary Structure 38
References TTr 41
Chapter 4
Biliproteins: Some Physical Properties 45
I Nomenclature 45
II Biliproteins 45
A C-Phycocyanin 45
B R-Phycocyanin 46
C C-Phycoeiythrin 46
D CU-Phycoerythrin 47
E R-Phycoerythrin 49
F B-Phycoerythrin 49
G b-Phycoerythrin 50
H Phycoerythrocyanin 50
I Allophycocyanin 51
J Allophycocyanin B 52
K Allophycocyanin I 53
L Cryptomonad Biliproteins 54
III C-Phycocyanin Aggregation 54
IV Aggregation-Spectra Relationship 58
V CD Spectroscopy of Biliproteins 61
VI Absorptivities 64
VII Denaturation and Renaturation 64
VIII X-Ray Crystallography and Electron Microscopy 65
IX Self- and Directed-Assembly 66
X Additional Studies 66
References 67
Chapter 5
Phycobilisome: Linker and Core Structures 73
I Discovery of the Linkers 73
II Linkers in Red Algae 73
III Functions of Linkers 74
A Linkers in Anacyslis nidulans 74
B Linkers in Synechocystis 6701 76
C Linkers in Nostoc sp 77
D Linkers in Phycoerythrocyanin-Containing Cyanobacteria 80
E Linkers in Red Algae 81
F Linkers? 81
IV Phycobilisomes and Complementary Chromatic Adaptation 81
V The 18 3K Biliprotein Core Subunit 86
VI The Core Structure 86
VII Amino Acid Sequence of a Linker 89
VIII Detergent-Free Phycobilisomes 90
IX Reassociation of Phycobilisomes 90
X Effects of Linkers on Spectra 90
XI Kinetics of Linker Appearance 91
XII Energy Transfer Within the Core 91
References 91
Chapter 6
Excitation Energy Transferrr 95
I Excitons 95
II Energy Transfer in Intact Organisms 96
III Energy Transfer in Isolated Phycobilisomes 97
IV Energy Transfer in Purified Biliproteins 99
A Fluorescence Polarization 99
B s and f Chromophores 100
C Fluorescence Polarization of Allophycocyanin 103
D Energy Transfer in Cryptomonad Biliproteins 103
V Picosecond Time-Related Studies on Intact Cyanobacteria and Red Algae 105
VI Picosecond Studies on Phycobilisomes 106
VII Picosecond Studies on Individual Biliproteins 108
A Ultrafast Studies of the s and f Chromophores 108
B Ultrafast Studies on C-Phycocyanin 108
C Ultrafast Studies on Cryptomonad Biliproteins 109
VIII Picosecond Studies on Isolated Biliprotein Subunits 113
IX Quantum Yields and Lifetimes 113
X Exciton Annihilation 115
XI Spectral Deconvolution 116
XII Photochemical Hole Burning 117
XIII Low-Temperature Fluorescence 118
References 119
Chapter 7
Physiology 125
I Biliprotein Content 125
II Effect of Growth Light on Biliproteins 125
A Background 125
B Growth in White Light 125
C Growth in Colored Light 126
D Growth Light and Photosynthesis 127
III Complementary Chromatic Adaptation 128
IV Far-Red Light Mutants 132
V Some Effects of Habitat 133
A Marine Ecology and Phycoerythrin 133
B Thermophiles 136
C Habitats of High Salt and Dehydration 136
VI Biliprotein Assays 137
VII Isolation of Photoreversible Pigments 138
VIII Nitrogen Chlorosis and Heterocysts 141
IX Bilin Synthesis 142
A Cyanidium caldarium 142
B Excreted Bilin 143
C A Heme Precursor 144
D A Biliverdin Precursor 145
E Other Approaches 146
X Aplysia 146
XI Biliprotein Synthesis 148
References 150
Chapter 8
Biliprotein-Thylakoid Interaction 157
I Light Harvesting by Two Photosystems 157
II Phycobilisomes and Chlorophyll a 159
A Photosystems I and II Light Harvesting 159
B Spillover 161
C Chlorophyll-Proteins 162
D Photosystem II-Phycobilisome Complexes 163
III Freeze-Fracture Particles 163
IV Number of Phycobilisomes per Reaction Center 169
V Cryptomonad Thylakoids 170
References 170
Chapter 9
Cryptomonads 175
I Cellular Features 175
II Biliproteins 175
A Distribution - 175
B Biliprotein Structure 180
C Chromophores 181
D Spectra 187
III Excitation Energy Transfer 189
A Intact Organisms 189
B Isolated Biliproteins 189
IV a Subunit Heterogeneity 190
References 191
Chapter 10
Biophysical and Immunochemical Techniques 193
I Deuterium Isotope Substitution and Solvent Isotope Effects 193
II Interface Studies in Model Systems 194
A Bilayer Lipid Membranes (BLM) 194
B Monolayer Studies on C-Phycocyanin 196
C Biliproteins on Solid Surfaces 197
III Thermodynamics of C-Phycocyanin Interactions 197
IV Immunochemical Studies 199
A Some Comments of Evolution 199
B Immunochemistry of the Biliproteins 200
C Amino Acid Sequencing 204
D Biliproteins in Fluoroimmunoassay 205
References 207
Index 211
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| spellingShingle | MacColl, Robert Guard-Friar, Deborah Phycobiliproteins Algae Composition Photosynthesis Phycobiliproteins Photosynthese (DE-588)4045936-6 gnd Phycobiliproteine (DE-588)4211941-8 gnd Algen (DE-588)4001173-2 gnd |
| subject_GND | (DE-588)4045936-6 (DE-588)4211941-8 (DE-588)4001173-2 |
| title | Phycobiliproteins |
| title_auth | Phycobiliproteins |
| title_exact_search | Phycobiliproteins |
| title_full | Phycobiliproteins authors Robert MacColl and Deborah Guard-Friar |
| title_fullStr | Phycobiliproteins authors Robert MacColl and Deborah Guard-Friar |
| title_full_unstemmed | Phycobiliproteins authors Robert MacColl and Deborah Guard-Friar |
| title_short | Phycobiliproteins |
| title_sort | phycobiliproteins |
| topic | Algae Composition Photosynthesis Phycobiliproteins Photosynthese (DE-588)4045936-6 gnd Phycobiliproteine (DE-588)4211941-8 gnd Algen (DE-588)4001173-2 gnd |
| topic_facet | Algae Composition Photosynthesis Phycobiliproteins Photosynthese Phycobiliproteine Algen |
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