High performance liquid chromatography in plant sciences:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Berlin [u.a.]
Springer
1987
|
| Schriftenreihe: | Modern methods of plant analysis
New series ; 5 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XX, 248 S. Ill. |
| ISBN: | 3540172432 0387172432 |
Internformat
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| 245 | 1 | 0 | |a High performance liquid chromatography in plant sciences |c ed. by H. F. Linskens ... Contributors: G. W. M. Barendse ... |
| 264 | 1 | |a Berlin [u.a.] |b Springer |c 1987 | |
| 300 | |a XX, 248 S. |b Ill. | ||
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| 490 | 1 | |a Modern methods of plant analysis : New series |v 5 | |
| 650 | 4 | |a Chromatographie en phase liquide à hautes performances | |
| 650 | 4 | |a Plantes - Analyse | |
| 650 | 4 | |a High performance liquid chromatography | |
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| adam_text | HIGH PERFORMANCE LIQUID CHROMATOGRAPHY IN PLANT SCIENCES EDITED BY
H.ELINSKENS AND J.E JACKSON CONTRIBUTORS G.W.M.BARENDSE CH.-M.CHEN
R.A.CREELMAN A.CROZIER P.J. DAVIES A.ERNSTSEN A.W. GALSTON R.D.HARTLEY
J.P.F.G.HELSPER L.E. HOOD S.B.H. KENT J.M.ROBERTSON G.SANDBERG L.A.SMITH
M.A.SMITH R.N. STRANGE R.SUTFELD B.SUNDBERG P.TEMPST G.A.THOMPSON, JR.
A.F. TIBURCIO J.A.D.ZEEVAART WITH 74 FIGURES SPRINGER-VERLAG BERLIN
HEIDELBERG NEW YORK LONDON PARIS TOKYO CONTENTS HIGH PERFORMANCE LIQUID
CHROMATOGRAPHY OF GIBBERELLINS G. W. M. BARENDSE (WITH 8 FIGURES) 1
INTRODUCTION 1 2 EXTRACTION AND PURIFICATION 5 3 GROUP SEPARATION
PROCEDURES 7 3.1 PVP ADSORPTION CHROMATOGRAPHY 7 3.2 GEL PERMEATION OR
STERIC EXCLUSION CHROMATOGRAPHY 7 3.3 ANION EXCHANGE CHROMATOGRAPHY 8
3.4 CHARCOAL ADSORPTION CHROMATOGRAPHY . 8 3.5 SEPHADEX G-10
CHROMATOGRAPHY 8 3.6 COUNTERCURRENT DISTRIBUTION 9 3.7 SILICA GEL COATED
DISPOSABLE EXTRACTION COLUMNS 9 4 HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
(HPLC) 9 4.1 GEL PERMEATION OR STERIC EXCLUSION HPLC 10 4.2 NORMAL PHASE
HPLC 11 4.3 REVERSED PHASE HPLC 12 4.4 DETECTION OF GIBBERELLINS AFTER
HPLC 14 5 RECENT DEVELOPMENTS AND PROSPECTS 19 5.1 DIODE ARRAY UV
DETECTION 20 5.2 ELECTROCHEMICAL DETECTION 20 5.3 COMBINED HPLC
IMMUNOASSAY 20 REFERENCES 21 CHARACTERIZATION OF CYTOKININS AND RELATED
COMPOUNDS BY HPLC CH.-M. CHEN (WITH 8 FIGURES) 1 INTRODUCTION 23 1.1
BACKGROUND 23 1.2 STRUCTURE AND PHYSICOCHEMICAL PROPERTIES OF CYTOKININS
AND RELATED COMPOUNDS 23 2 CYTOKININ ISOLATION AND SAMPLE PREPARATION 25
2.1 CYTOKININ ISOLATION 25 2.2 SAMPLE PREPARATION 27 3 PREPARATION OF
MOBILE PHASE 27 3.1 WATER 27 3.2 ORGANIC SOLVENTS 27 4 CHROMATOGRAPHY 28
4.1 COLUMN SELECTION 28 4.2 SEPARATION EXAMPLES 28 X CONTENTS 4.2.1 ION
EXCHANGE HPLC 28 4.2.2 NORMAL PHASE HPLC 31 4.2.3 REVERSE PHASE HPLC 33
4.2.4 ION-PAIR REVERSE PHASE HPLC 35 5 CONCLUDING REMARKS 37 REFERENCES
37 SEPARATION AND PURIFICATION OF ABSCISIC ACID AND ITS CATABOLITES BY
HIGH PERFORMANCE LIQUID CHROMATOGRAPHY R. A. CREELMAN AND J. A. D.
ZEEVAART (WITH 5 FIGURES) 1 INTRODUCTION 39 2 ABSCISIC ACID AND ITS
CATABOLITES 41 3 USE OF HIGH PERFORMANCE LIQUID CHROMATOGRAPHY TO
CHARACTERIZE ABSCISIC ACID AND ITS CATABOLITES 44 3.1 SYNTHESIS OF ( +
)-ABSCISIC ACID AND CATABOLITES 44 3.2 RESOLUTION OF ( )-ABSCISIC ACID
44 3.3 METHODS FOR THE PURIFICATION OF ABSCISIC ACID AND CATABOLITES BY
HIGH PERFORMANCE LIQUID CHROMATOGRAPHY 45 4 CONCLUDING REMARKS 48
REFERENCES 49 THE DETERMINATION OF ABSCISIC ACID BY HIGH PERFORMANCE
LIQUID CHROMATOGRAPHY J.M. ROBERTSON 1 INTRODUCTION 52 1.1 HPLC AND ABA
52 1.1.1 WHY HAVE MANY DIFFERENT METHODS BEEN USED? 52 1.1.2 WHICH
METHOD IS CORRECT? HOW TO CHOOSE 52 1.1.3 GENERAL APPROACH TO HPLC OF
ABA 53 1.2 HPLC GENERAL 53 1.2.1 THEORETICAL CONSIDERATIONS 53 1.2.1.1
RETENTION 53 1.2.1.2 BAND SPREADING 54 1.2.1.3 RESOLUTION 54 1.2.1.4
CONTROLLING R S BY N, K , AND A 55 1.2.2 PREPARATIVE VERSUS ANALYTICAL
OPERATION 56 1.2.3 ELUTION 56 1.2.4 THE DIFFERENT MODES OF HPLC 58
1.2.4.1 PARTITION OR LIQUID-LIQUID CHROMATOGRAPHY (LLC) . 58 1.2.4.2
ADSORPTION OR LIQUID-SOLID CHROMATOGRAPHY (LSC) . 58 1.2.4.3 SIZE
EXCLUSION CHROMATOGRAPHY (SEC) 59 1.2.4.4 ION EXCHANGE CHROMATOGRAPHY
(IEC) 59 1.2.4.5 BONDED-PHASE CHROMATOGRAPHY (BPC) 60 1.2.5 DETECTION 60
1.3 ABA GENERAL 61 1.3.1 THE ABA MOLECULE 61 CONTENTS XI 1.3.2
EXTRACTION AND PREPARATION . . ; 62 1.3.3 DETERMINATION OF ABA 62 1.4
GENERALIZED SCHEME FOR THE SEPARATION OF ABA 63 1.4.1 CHOOSING THE
COLUMNS 64 1.4.2 TYPING THE SYSTEM 64 1.4.3 EXTRACTION AND PREPARATION
FOR RPLC 65 1.4.4 THE SECOND COLUMN 67 1.4.5 CHROMATOGRAPHY OF ABA IN
CONJUNCTION WITH OTHER COMPOUNDS 68 1.4.6 SUMMARY OF GENERAL SEPARATION
SCHEME 68 REFERENCES 68 HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND THE
ANALYSIS OF INDOLE-3-ACETIC ACID, AND SOME OF ITS DECARBOXYLATED
CATABOLITES IN SCOTS PINE (PINUS SYLVESTRIS L.) G. SANDBERG, A. CROZIER,
A. ERNSTSEN, AND B. SUNDBERG (WITH 11 FIGURES) 1 INTRODUCTION 72 2
INTERNAL STANDARDIZATION 74 3 EXTRACTION AND PURIFICATION 76 4 HIGH
PERFORMANCE LIQUID CHROMATOGRAPHY 77 5 EXAMPLES 79 5.1 INDOLE-3-ACETIC
ACID 79 5.1.1 EXTRACTION 79 5.1.2 PURIFICATION. 79 5.1.2.1 PVP, XAD-7
AND SEP-PAK 80 5.1.2.2 IMMUNOAFFINITY CHROMATOGRAPHY 80 5.1.3 HPLC
ANALYSIS 81 5.2 INDOLE-3-CARBOXYLIC ACID 83 5.2.1 EXTRACTION AND
PURIFICATION 83 5.2.2 HPLC ANALYSIS . 84 5.2.3 INTERNAL STANDARDS . 85
5.3 INDOLE-3-METHANOL 86 5.3.1 EXTRACTION AND PURIFICATION 86 5.3.2 HPLC
ANALYSIS 87 5.3.3 INTERNAL STANDARDS 88 6 CONCLUSIONS 89 REFERENCES 89
HPLC FOR THE SEPARATION AND DETERMINATION OF PHENOLIC COMPOUNDS IN PLANT
CELL WALLS R. D. HARTLEY (WITH 2 FIGURES) 1 INTRODUCTION 92 2 GENERAL
CONSIDERATIONS 94 3 THE RECOMMENDED PROCEDURE - ISOLATION OF CELL WALLS
95 3.1 REAGENTS AND EQUIPMENT 95 XII CONTENTS 3.1.1 REAGENTS (ALL
ANALYTICAL GRADE) 95 3.1.2 EQUIPMENT 95 3.2 ISOLATION OF CELL WALLS 96
3.3 ISOLATION OF DIETARY FIBRE 96 4 THE RECOMMENDED PROCEDURE - RELEASE
OF PHENOLIC ACIDS AND ALDEHYDES - FROM CELL WALLS AND THEIR SEPARATION
AND DETERMINATION BY HPLC (C 18 REVERSE PHASE) (METHODS 1 AND 2) 97 4.1
REAGENTS AND EQUIPMENT 97 4.1.1 REAGENTS (ALL ANALYTICAL GRADE) 97 4.1.2
EQUIPMENT 97 4.2 RELEASE OF PHENOLIC ACIDS AND ALDEHYDES FROM CELL WALLS
AND PREPARATION OF SOLUTIONS FOR ANALYTICAL HPLC (C 18 REVERSE PHASE) 97
4.3 ANALYTICAL HPLC (CJ 8 REVERSE PHASE) OF PHENOLIC ACIDS AND ALDEHYDES
(METHODS 1 AND 2) 97 5 ALTERNATIVE PROCEDURE - RELEASE OF PHENOLIC ACIDS
AND ALDEHYDES FROM CELL WALLS AND THEIR SEPARATION AND DETERMINATION BY
HPLC (PRP-1 RESIN) (METHODS 3 AND 4) 98 5.1 REAGENTS AND EQUIPMENT 98
5.2 RELEASE OF PHENOLIC ACIDS AND ALDEHYDES FROM CELL WALLS AND
PREPARATION OF SOLUTIONS FOR HPLC (PRP-1 RESIN) 98 5.2.1 METHOD FOR
ANALYTICAL HPLC (PRP-1 RESIN) 98 5.2.2 METHOD FOR PREPARATIVE HPLC
(PRP-1 RESIN) 99 5.3 ANALYTICAL HPLC (PRP-1 RESIN) OF THE PHENOLIC ACIDS
AND ALDEHYDES 99 5.3.1 PHENOLIC ALDEHYDES AND TRANS,TRANS-DIFERULIC ACID
(METHOD3) 99 5.3.2 P-COUMARIC AND FERULIC ACIDS (METHOD 4) 99 5.4
PREPARATIVE HPLC (PRP-1 RESIN) OF THE PHENOLIC ACIDS AND ALDEHYDES 101
5.4.1 PREPARATION OF THE ALDEHYDES AND TRANS,TRANS-DIFERULIC ACID . 101
5.4.2 PREPARATION OF P-COUMARIC AND FERULIC ACIDS 101 6 AMOUNT OF
PHENOLIC ACIDS AND ALDEHYDES RELEASED FROM THE CELL WALLS OF
GRAMINACEOUS PLANTS 101 REFERENCES * * * 101 HPLC OF THIOPHENES FOR
PHYTOCHEMICAL AND BIOCHEMICAL RESEARCH R. SUTFELD (WITH 6 FIGURES) 1
INTRODUCTION 104 2 MATERIAL AND METHODS . 105 3 USE OF HPLC FOR
PHYTOCHEMICAL RESEARCH ON THIOPHENES 106 3.1 PRELIMINARY EXPERIMENTS:
HPLC OF COMMERCIALLY AVAILABLE REFERENCE SUBSTANCES 106 3.2 HPLC OF
NATURALLY OCCURRING THIOPHENES 107 3.2.1 THIOPHENES FROM TAGETES PATULA
SEEDLINGS 107 3.2.2 THIOPHENES FROM OTHER PLANT SOURCES 109 4 USE OF
HPLC FOR ENZYMATIC RESEARCH ON THIOPHENES ILL 5 CONCLUSIONS 112
REFERENCES 112 CONTENTS XIII HIGH PERFORMANCE LIQUID CHROMATOGRAPHY OF
ASCORBIC ACID J. P. F. G. HELSPER 1 INTRODUCTION 114 2 SAMPLE
PREPARATION 114 3 STATIONARY AND MOBILE PHASES 115 3.1 SEPARATION OF
ASCORBIC ACID FROM DEHYDROASCORBIC ACID AND FROM REDUCING AGENTS 115 3.2
SEPARATION OF ASCORBIC ACID AND METABOLICALLY RELATED COMPOUNDS 116 3.3
SEPARATION OF ISOMERIC FORMS OF ASCORBIC ACID 117 4 DETECTION TECHNIQUES
FOR ASCORBIC ACID 117 REFERENCES 119 HIGH PERFORMANCE LIQUID
CHROMATOGRAPHY OF PHYTOALEXINS R. N. STRANGE (WITH 13 FIGURES) 1
INTRODUCTION 121 2 INSTRUMENTATION 122 3 PREPARATIVE HPLC OF
PHYTOALEXINS 125 3.1 PRINCIPLES 125 3.1.1 ELICITATION 125 3.1.2
EXTRACTION AND CLEAN UP 126 3.1.3 CHROMATOGRAPHY 127 3.2 PRACTICE 128
3.2.1 ISOLATION OF STILBENE PHYTOALEXINS FROM KERNELS 128 3.2.2
ISOLATION OF HYDROXYFLAVAN PHYTOALEXINS FROM NARCISSUS BULBS 129 3.2.3
ISOLATION OF PTEROCARPAN PHYTOALEXINS (GLYCEOLLINS) FROM SOYBEAN
COTYLEDONS 130 3.2.4 ISOLATION OF ISOFLAVONE AND ISOFLAVANONE
PHYTOALEXINS FROM PIGEONPEA SEEDS 132 3.2.5 ISOLATION OF CHALCONE AND
STILBENE PHYTOALEXINS FROM PIGEONPEA LEAVES . 133 4 ANALYTICAL HPLC OF
PHYTOALEXINS 134 4.1 PRINCIPLES 134 4.1.1 ELICITATION 134 4.1.2 SAMPLING
134 4.1.3 EXTRACTION AND CLEAN UP .135 4.1.4 CHROMATOGRAPHY 136 4.2
PRACTICE 137 4.2.1 ANALYTICAL HPLC OF FURANOACETYLENIC PHYTOALEXINS FROM
BROAD BEAN 137 4.2.2 ANALYTICAL HPLC OF PTEROCARPAN PHYTOALEXINS
(GLYCEOLLINS) FROM SOYBEAN 139 4.2.3 ANALYTICAL HPLC OF PHYTOALEXINS
FROM POTATO 141 4.2.4 ANALYTICAL HPLC OF PHYTOALEXINS FROM FRENCH BEAN .
. . . 142 4.2.5 ANALYTICAL HPLC OF PHYTOALEXINS FROM COTTON 142 XIV
CONTENTS 5 APPLICATION OF HPLC TO THE SOLUTION OF OUTSTANDING PROBLEMS
IN PHYTOALEXIN RESEARCH 144 5.1 ASSESSMENT OF THE ROLE OF PHYTOALEXINS
IN RESISTANCE TO MICROBIAL ATTACK 144 5.2 MEASUREMENT OF THE PHYTOALEXIN
POTENTIAL OF THE PLANT 145 5.3 THE ELICITATION, BIOSYNTHESIS AND
DEGRADATION OF PHYTOALEXINS . . 145 6 CONCLUDING REMARKS 146 REFERENCES
146 ANALYSIS OF LIPIDS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY L. A.
SMITH AND G. A. THOMPSON, JR. (WITH 4 FIGURES) 1 INTRODUCTION 149 2
INSTRUMENTATION 150 2.1 PUMPS, INJECTORS, AND COLUMNS 150 2.2 DETECTORS
150 2.3 MOBILE PHASE SELECTION 154 3 LIPID HPLC APPLICATIONS 156 3.1
INITIAL LIPID EXTRACTION AND PURIFICATION 156 3.1.1 LIPID EXTRACTION 156
3.1.1.1 COMMENTS 156 3.1.1.2 PROCEDURE 156 3.2 SEPARATION OF LIPID
CLASSES 157 3.2.1 HPLC SEPARATION OF LIPID CLASSES 157 3.2.2 SEPARATION
OF LIPID CLASSES BY OTHER TECHNIQUES 158 3.2.2.1 COLUMN CHROMATOGRAPHY
158 3.2.2.2 THIN LAYER CHROMATOGRAPHY 159 3.3 HPLC ANALYSIS OF
INDIVIDUAL MOLECULAR SPECIES OF LIPID CLASSES 160 3.3.1 SEPARATION OF
PHOSPHOLIPID MOLECULAR SPECIES BY HPLC OF DIGLYCERIDE DERIVATIVES 160
3.3.1.1 PROCEDURE 161 3.3.1.2 COMMENTS 161 3.3.2 HPLC SEPARATION OF
PHOSPHATIDYLGLYCEROL MOLECULAR SPECIES 161 3.3.2.1 SAMPLE PREPARATION
161 3.3.2.2 INSTRUMENTATION AND MOBILE PHASES 162 3.3.3 HPLC SEPARATION
OF GLYCOLIPID MOLECULAR SPECIES 163 3.3.3.1 SAMPLE PREPARATION : . 163
3.3.3.2 INSTRUMENTATION AND MOBILE PHASE 163 3.3.4 FATTY ACID ANALYSIS
164 3.3.4.1 PRODUCTION OF FREE FATTY ACIDS FROM LIPID CLASSES . 164
3.3.4.2 PREPARATION OF FATTY ACID DERIVATIVES 164 3.3.4.3 HPLC ANALYSIS
OF NITROPHENACYL FATTY ACID DERIVATIVES 164 3.3.4.4 TRACE ANALYSIS OF
FATTY ACID BY HPLC 166 3.3.5 HPLC OF OTHER LIPID CLASSES 166 REFERENCES
167 CONTENTS XV PRACTICAL HIGH PERFORMANCE LIQUID CHROMATOGRAPHY OF
PROTEINS AND PEPTIDES P. TEMPST, L. E. HOOD, AND S. B. H. KENT (WITH 7
FIGURES) 1 INTRODUCTION 170 2 SIZE EXCLUSION HPLC 171 2.1 COLUMNS 172
2.1.1 PACKINGS AND SURFACE INTERACTIONS 172 2.1.2 FRACTIONATION LIMITS
172 2.2 OPERATIONAL PARAMETERS AND RESOLUTION 174 2.2.1 MOBILE PHASE 174
2.2.2 SAMPLE 176 2.2.3 MOBILE PHASE VELOCITY 176 2.3 PRACTICAL
APPLICATIONS 176 2.3.1 MOLECULAR WEIGHT DETERMINATION 176 2.3.2
PRESERVATION OF BIOLOGICAL ACTIVITY 177 2.3.3 SELECTED APPLICATIONS 177
2.4 LARGE-SCALE SEPARATIONS 178 2.5 MAINTENANCE 179 3 ION EXCHANGE HPLC
179 3.1 COLUMNS 180 3.2 OPERATIONAL PARAMETERS AND RESOLUTION 181 3.2.1
MOBILE PHASE 181 3.2.2 MOBILE PHASE VELOCITY 183 3.2.3 GRADIENT SLOPE
183 3.2.4 SAMPLE AND LOAD 183 3.3 SELECTED APPLICATIONS 184 4 REVERSED
PHASE HPLC 185 4.1 COLUMNS 186 4.2 OPERATIONAL PARAMETERS AND RESOLUTION
192 4.2.1 MOBILE PHASE 192 4.2.1.1 ORGANIC SOLVENT 192 4.2.1.2 MOBILE
PHASE PH ; . . 193 4.2.1.3 ION-PAIRING AGENTS .194 4.2.1.4 OTHER AQUEOUS
SOLVENT COMPOSITIONS 195 4.2.1.5 ACID CONCENTRATION 195 4.2.2 MOBILE
PHASE VELOCITY AND TEMPERATURE 196 4.2.3 GRADIENT SLOPE 196 4.2.4 SAMPLE
COMPOSITION AND LOAD .197 4.2.5 PREDICTION OF RETENTION TIMES 198 4.3
HIGH SENSITIVITY RP-HPLC 198 4.4 APPLICATIONS 199 4.4.1 TWO-DIMENSIONAL
RP-HPLC 199 4.4.2 HYDROPHOBIC POLYPEPTIDES 200 4.4.3 OTHERS 201 4.5
MAINTENANCE 202 5 MULTIDIMENSIONAL HPLC 202 REFERENCES 204 XVI CONTENTS
MONITORING POLYAMINES IN PLANT TISSUES BY HIGH PERFORMANCE LIQUID
CHROMATOGRAPHY M. A. SMITH AND P. J. DA VIES (WITH 7 FIGURES) 1
INTRODUCTION 209 2 HIGH PERFORMANCE LIQUID CHROMATOGRAPHY OF
DANSYLAMINES . . . . : 210 2.1 DANSYL DERIVATIVES 210 2.2 REAGENTS AND
STOCK SOLUTIONS 211 2.3 EXTRACTION OF POLYAMINES 212 2.4 PURIFICATION OF
EXTRACTS 212 2.5 DANSYLATION OF AMINES IN A SAMPLE 213 2.6
CHROMATOGRAPHIC SEPARATION OF DANSYLAMINES 214 2.7 QUANTIFICATION 216 3
HIGH PERFORMANCE LIQUID CHROMATOGRAPHY OF BENZOYLAMINES . . . .217 3.1
BENZOYLATION OF AMINES IN A SAMPLE 218 3.2 CHROMATOGRAPHIC SEPARATION OF
BENZOYLAMINES 218 4 ION-PAIR REVERSE PHASE CHROMATOGRAPHY OF
UNDERIVATIZED POLYAMINES 219 4.1 O-PHTHALALDEHYDE DERIVATIVES 220 4.2
SAMPLE PREPARATION 220 4.3 CHROMATOGRAPHIC SEPARATION OF UNDERIVATIZED
POLYAMINES . . . . 221 4.4 POST-COLUMN DERIVATIZATION WITH OPA 221 4.5
DETECTION OF OPA DERIVATIVES 221 5 ION-EXCHANGE CHROMATOGRAPHY OF
UNDERIVATIZED POLYAMINES . . . . 222 5.1 CHROMATOGRAPHIC SEPARATION OF
UNDERIVATIZED AMINES AND RELATED COMPOUNDS 222 6 CONCLUSION 223
REFERENCES 224 ANALYSIS OF ALKALOIDS IN TOBACCO CALLUS BY HPLC A. F.
TIBURCIO AND A. W. GALSTON (WITH 3 FIGURES) 1 INTRODUCTION 228 1.1
BACKGROUND 228 1.2 ANALYTICAL METHODS 229 2 EXPERIMENTAL 230 2.1 PLANT
MATERIAL 230 2.1.1 EXPLANT ORIGIN AND CALLUS INDUCTION 230 2.1.2 CALLUS
CULTURE 230 2.2 ALKALOID EXTRACTION . 231 2.2.1 IN DRY TISSUE 231 2.2.2
IN FRESH TISSUE 231 2.3 ANALYSIS BY HPLC 231 2.3.1 SAMPLE PREPARATION
AND PURIFICATION OF STANDARDS 231 2.3.2 INSTRUMENTATION AND SEPARATION
OF ALKALOIDS 232 2.3.3 ALKALOID QUANTITATION. CALIBRATION CURVES 232 3
RESULTS AND DISCUSSION 232 3.1 CALLUS CULTURE METHOD 232 3.2 ALKALOID
EXTRACTION 233 CONTENTS XVII 3.3 ANALYSIS BY HPLC 234 3.4 APPLICATIONS
TO THE STUDY OF ALKALOID PRODUCTION AND METABOLISM IN VITRO 236 3.4.1 IN
VITRO BIOSYNTHESIS OF NORNICOTINE 236 3.4.2 EFFECT OF ORGANIC ACIDS ON
ALKALOID CONTENT 237 3.4.3 EFFECT OF PUTRESCINE BIOSYNTHETIC INHIBITORS
ON ALKALOID CONTENT 238 4 CONCLUSIONS 239 REFERENCES 240 SUBJECT INDEX
243
|
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| discipline | Chemie / Pharmazie Biologie Chemie |
| format | Book |
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| id | DE-604.BV000681250 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T15:17:42Z |
| institution | BVB |
| isbn | 3540172432 0387172432 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-000425039 |
| oclc_num | 15163489 |
| open_access_boolean | |
| owner | DE-12 DE-M49 DE-BY-TUM DE-703 DE-355 DE-BY-UBR DE-20 DE-29T DE-19 DE-BY-UBM DE-Eb1 DE-83 DE-11 DE-188 |
| owner_facet | DE-12 DE-M49 DE-BY-TUM DE-703 DE-355 DE-BY-UBR DE-20 DE-29T DE-19 DE-BY-UBM DE-Eb1 DE-83 DE-11 DE-188 |
| physical | XX, 248 S. Ill. |
| publishDate | 1987 |
| publishDateSearch | 1987 |
| publishDateSort | 1987 |
| publisher | Springer |
| record_format | marc |
| series | Modern methods of plant analysis |
| series2 | Modern methods of plant analysis : New series |
| spelling | High performance liquid chromatography in plant sciences ed. by H. F. Linskens ... Contributors: G. W. M. Barendse ... Berlin [u.a.] Springer 1987 XX, 248 S. Ill. txt rdacontent n rdamedia nc rdacarrier Modern methods of plant analysis : New series 5 Chromatographie en phase liquide à hautes performances Plantes - Analyse High performance liquid chromatography Plants Analysis HPLC (DE-588)4072641-1 gnd rswk-swf Pflanzenanalyse (DE-588)4174053-1 gnd rswk-swf Pflanzeninhaltsstoff (DE-588)4139067-2 gnd rswk-swf Botanik (DE-588)4007842-5 gnd rswk-swf HPLC (DE-588)4072641-1 s Pflanzenanalyse (DE-588)4174053-1 s DE-604 Pflanzeninhaltsstoff (DE-588)4139067-2 s Botanik (DE-588)4007842-5 s Linskens, Hans F. 1921-2007 Sonstige (DE-588)139347585 oth Barendse, Gerhard W. M. 1934- Sonstige (DE-588)123050669 oth Modern methods of plant analysis New series ; 5 (DE-604)BV035361205 5 GBV Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=000425039&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | High performance liquid chromatography in plant sciences Modern methods of plant analysis Chromatographie en phase liquide à hautes performances Plantes - Analyse High performance liquid chromatography Plants Analysis HPLC (DE-588)4072641-1 gnd Pflanzenanalyse (DE-588)4174053-1 gnd Pflanzeninhaltsstoff (DE-588)4139067-2 gnd Botanik (DE-588)4007842-5 gnd |
| subject_GND | (DE-588)4072641-1 (DE-588)4174053-1 (DE-588)4139067-2 (DE-588)4007842-5 |
| title | High performance liquid chromatography in plant sciences |
| title_auth | High performance liquid chromatography in plant sciences |
| title_exact_search | High performance liquid chromatography in plant sciences |
| title_full | High performance liquid chromatography in plant sciences ed. by H. F. Linskens ... Contributors: G. W. M. Barendse ... |
| title_fullStr | High performance liquid chromatography in plant sciences ed. by H. F. Linskens ... Contributors: G. W. M. Barendse ... |
| title_full_unstemmed | High performance liquid chromatography in plant sciences ed. by H. F. Linskens ... Contributors: G. W. M. Barendse ... |
| title_short | High performance liquid chromatography in plant sciences |
| title_sort | high performance liquid chromatography in plant sciences |
| topic | Chromatographie en phase liquide à hautes performances Plantes - Analyse High performance liquid chromatography Plants Analysis HPLC (DE-588)4072641-1 gnd Pflanzenanalyse (DE-588)4174053-1 gnd Pflanzeninhaltsstoff (DE-588)4139067-2 gnd Botanik (DE-588)4007842-5 gnd |
| topic_facet | Chromatographie en phase liquide à hautes performances Plantes - Analyse High performance liquid chromatography Plants Analysis HPLC Pflanzenanalyse Pflanzeninhaltsstoff Botanik |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=000425039&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035361205 |
| work_keys_str_mv | AT linskenshansf highperformanceliquidchromatographyinplantsciences AT barendsegerhardwm highperformanceliquidchromatographyinplantsciences |