Carbohydrate metabolism in cultured cells:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York u.a.
Plenum Pr.
1986
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XXI, 514 S. |
| ISBN: | 0306422409 |
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| 245 | 1 | 0 | |a Carbohydrate metabolism in cultured cells |c ed. by Michael J. Morgan |
| 264 | 1 | |a New York u.a. |b Plenum Pr. |c 1986 | |
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| 650 | 4 | |a Cellules - Métabolisme | |
| 650 | 4 | |a Glucides - Métabolisme | |
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| 650 | 4 | |a Carbohydrates |x Metabolism | |
| 650 | 4 | |a Cell culture | |
| 650 | 4 | |a Cell metabolism | |
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Datensatz im Suchindex
| _version_ | 1804115060842299392 |
|---|---|
| adam_text | Carbohydrate
Metabolism
in Cultured Cells
Edited by
Michael J Morgan
University of Leicester
Leicester, England
Plenum Press • New York and London
Contents
Chapter 1
Studies of Regulation ofHexose Transport into Cultured Fibroblasts
HERMAN M KALCKAR AND DONNA B ULLREY
1 Introduction 1
2 Hexose Uptake or Transport Tests 2
2 1 Effects of Cycloheximide on Hexose Transport Regulation 4
2 2 Is the Release of the Mediated Curb of the Hexose Transport
System Dependent on Transcription? 6
3 Metabolic Pathways 6
4 Characterization of Certain Cellular Macromolecules and
Structures - 11
4 1 Enzyme Assays of the Hexose Uptake System in Lysed Cells
4 2 Membrane-Associated Glucose-Binding Proteins Released
without Cell Lysis 12
4 3 Studies on Isolated Plasma Membrane Preparations in Regard
to Their Hexose Transport Population and Identification of
Specific Transport Proteins 13
5 Effects of Glucose Starvation on a Variety of Plasma Membrane
Proteins 15
6 The Glucose-Mediated Curb of Hexose Transport Requires
Oxidative Energy 16
7 Nucleoside Triphosphate Levels in Cultured Fibroblasts as a
Function of General Metabolism and Nutrition 17
xiii
xiv CONTENTS
8 Hexose Transport Regulation and Oncogenic Transformation of
Cultured Fibroblasts 19
9 Evolutionary Aspects 22
References 23
Chapter 2
The Utilization of Carbohydrates by Animal Cells: An Approach to
Their Biochemical Genetics
MICHAEL J MORGAN AND PELIN FAIK
1 The Utilization of Carbohydrates 29
2 Glycolysis 38
2 1 Glucokinase 38
2 2 Hexokinase 39
2 3 Phosphoglucose Isomerase 40
2 4 Phosphofructokinase 40
2 5 Glyceraldehyde-3-pKbsphate Dehydrogenase 42
2 6 Phosphoglycerate Kinase 43
2 7 Other Glycolytic Enzymes 44
3 The Provision of Energy 45
3 1 Pyruvate Metabolism 46
4 Pentose Phosphate Pathway 47
4 1 Glucose-6-phosphate Dehydrogenase 49
5 Differentiation 51
5 1 Alternative Carbon Sources 53
5 2 Gluconeogenesis 63
6 Other Effects of Carbohydrates 66
6 1 Effects on Morphology 66
6 2 Glucose-Regulated Proteins 67
6 3 Hypergravity 68
7 Concluding Remarks 68
References 69
Chapter 3
Biochemical Genetics of Respiration-Deficient Mutants of Animal Cells
IMMO E SCHEFFLER
1 Introduction 77
2 The Selection of Respiration-Deficient Mammalian Cell Mutants 79
CONTENTS xv
2 1 Characterization of the First Mutant 79
2 2 Protocol for the Isolation of Additional Mutants 84
3 Glycolysis and Respiration in Wild-Type Parents and res~
Mutants 86
4 Biochemical Characterization of Mutants 89
4 1 Defect in NADH-CoQ Reductase 89
4 2 Defect in Succinate Dehydrogenase 92
4 3 Defect in Mitochondrial Protein Synthesis 93
5 Genetic Characterization of Mutants 99
6 Work in Progress and Future Prospects 102
7 Summary 105
References 106
Chapter 4
Glutaminolysis in Animal Cells
WALLACE L MCKEEHAN
1 Glutamine Metabolism in Mammals I l l
2 Glutaminolysis in Tissues 112
2 1 Liver and Kidney 112
2 2 Brain 112
2 3 Pancreas 113
2 4 Mammary Gland 113
2 5 The Intestine 113
2 6 Embryonic and Placental Tissue 114
2 7 Tumors 114
3 Glutaminolysis in Isolated Tissues and Primary Cell Suspensions 115
3 1 Enterocytes 115
3 2 Blood Cells 116
3 3 Lens 116
3 4 Germ Cells 117
3 5 Calvaria 117
3 6 Astrocytes 117
4 Glutaminolysis in Normal and Tumor Cells in Culture 118
4 1 Tumor-Derived Cells and Transformed Cell Lines 118
4 2 Untransformed Normal Cells in Culture 119
5 Glutaminolysis—The Pathway of Glutamine Oxidation 121
5 1 Enzymology 121
5 2 Compartmentation 129
5 3 Regulation of Glutaminolysis 130
ivi CONTENTS
6 Glutaminolysis and Glycolysis in Cell Growth and Function 132
6 1 The Role of Glycolysis in Cell Proliferation 133
6 2 Can Cells Proliferate in the Absence of Glutaminolysis? 134
6 3 The Role of Glutaminolysis in Cell Specialization 136
6 4 Potential Impact of Tumor Glutaminolysis on Host Glutamine
and Glucose Metabolism 136
7 Conclusions 138
8 Addendum 139
References 139
Chapter 5
The Metabolism and Utilization of Carbohydrates by Suspension
Cultures of Plant Cells
GAGIK STEPAN-SARKISSIAN AND MICHAEL W FOWLER
1 Introduction 151
2 Carbon Sources for Culture Growth 152
2 1 The Range of Carbon Sources Tested 152
2 2 Effects on Growth (Physiology and Biochemistry) 159
2 3 Effects on Natural Product Synthesis 162
3 Uptake Mechanisms for Carbon Sources 165
3 1 Differential Mechanisms 166
3 2 Cellular Location 168
3 3 Effect of Internal Pools 170
4 Intracellular Fate of Carbon Source—Biochemistry: Oxidation,
Biosynthesis, Storage 171
5 Summary Comments 175
References 175
Chapter 6
Carbohydrate Metabolism in African Trypanosomes, with Special
Reference to the Glycosome
ALAN H FAIRLAMB AND FRED R OPPERDOES
1 Introduction 183
1 1 General Background and Scope 183
1 2 Biology of the Kinetoplastida 184
CONTENTS • xvii
1 3 Medical and Economic Importance of African
Trypanosomiasis 185
2 Life Cycle 186
3 Methods for Cultivation 188
3 1 Mammalian Forms of T brucei 188
3 2 Insect Forms of T brucei 190
4 Substrates and End Products of Metabolism 190
4 1 Mammalian Forms 191
4 2 Insect Forms of the brucei Subgroup 194
5 Terminal Respiratory Systems 195
5 1 Mammalian Forms 195
5 2 Insect Forms 197
6 The Glycosome 199
6 1 Occurrence 199
6 2 Isolation and Purification 199
6 3 General Properties 200
6 4 Glycosomal Enzymes in T brucei 203
7 Pathways of Glucose Metabolism 205
7 1 Long-Slender Trypomastigotes 205
7 2 Short-Stumpy Trypomastigotes 209
7 3 Procyclic Trypomastigotes 209
8 Glycolysis as a Target for Chemotherapy 213
8 1 Energy Metabolism 213
8 2 Action of Trivalent Aromatic Arsenicals 214
8 3 Action of Suramin 214
8 4 Action of SHAM-Glycerol 215
9 Summary and Outlook 216
References 217
Chapter 7
Sugar Transport Systems of Baker s Yeast and Filamentous Fungi
ANTONIO H ROMANO
1 Yeast 225
1 1 Monosaccharide Transport Systems 232
1 2 Disaccharide Transport Systems 232
2 Filamentous Fungi 234
2 1 Aspergillus nidulans 234
2 2 Neurospora crassa 237
References 241
xviii CONTENTS
Chapter 8
Carbohydrate Metabolism in Yeast
JUANA M GANCEDO
1 Introduction 245
2 Methodological Approaches 246
2 1 Determination of Enzymatic Activities 246
2 2 Determination of Metabolite Levels 247
2 3 Fate of Labeled Substrates: Calculation of the Proportion of
Carbohydrates Used through Different Pathways 249
2 4 Continuous Cultures versus Batch Cultures 250
2 5 Use of Mutants 251
3 Overview of the Pathways of Carbohydrate Metabolism 254
3 1 Glycolytic Pathway 255
3 2 Metabolism of Carbohydrates Other Than Glucose 262
3 3 Pentose Phosphate Pathway 263
3 4 Citric Acid Cycle 265
3 5 Reserve Carbohydrates 266
4 Regulatory Mechanisms 268
4 1 Catabolite Repression 269
4 2 Catabolite Inactivation 270
4 3 Pasteur Effect and Other Effects 271
4 4 Futile Cycles 273
5 Conclusions 274
References 275
Chapter 9
Regulation of Carbon Metabolism in Filamentous Fungi
WILLIAM MCCULLOUGH, CLIVE F ROBERTS, STEPHEN A
OSMANI, AND MICHAEL C SCRUTTON
1 Introduction 287
2 Extracellular Formation of Hexoses from Polysaccharides 292
2 1 Cellulose Degradation 292
2 2 Hemicellulose (Xylan) Degradation 297
2 3 Starch Degradation 298
3 Growth on Glucose and Related Carbon Sources 299
3 1 General Considerations 299
CONTENTS • xix
3 2 Enzymes of Hexose Metabolism 304
3 3 Synthesis of Reserve Carbohydrates 310
3 4 Pathways of Overflow Metabolism and Their Control 314
4 Growth on Three-Carbon Substrates 324
4 1 Glycerol : 324
4 2 Pyruvate and Related Substrates 327
5 Growth on Acetate or Ethanol 328
5 1 General Considerations 328
5 2 Enzymes of Acetate or Ethanol Metabolism 333
6 Growth on Substrates Utilized via the Tricarboxylic Acid Cycle 337
7 Conclusions 340
References 340
Chapter 10
The Bacterial Phosphoenolpyruvate: Sugar Phosphotransferase System
of Escherichia coli and Salmonella typhimurium
PlETER W POSTMA
1 Introduction 357
2 The PEP:Sugar PTS: An Overview 358
3 Components of the PTS 360
3 1 Soluble Proteins 360
3 2 Membrane-Bound PTS Proteins 363
3 3 Levels of PTS Proteins 365
4 In Vitro Phosphorylation of Sugars by the PTS 366
5 Transport via the PTS 367
6 Role of the PTS in Chemotaxis 372
7 Genetics of the PTS 373
8 Phenotype of PTS Mutants 374
9 Regulation by the PTS 376
9 1 The err Gene 377
9 2 Adenylate Cyclase 380
9 3 In Vitro Reconstitution of the Regulatory System 381
9 4 Regulation of Carbohydrate Uptake in Vivo by the PTS 387
9 5 Other Mutations That Suppress the pts Phenotype 387
96A Model Involving Stoichiometric Interactions between PTS
and Non-PTS Components 390
9 7 Growth in Batch Cultures versus Chemostat Cultures 392
9 8 Final Remarks on PTS-Mediated Regulation 393
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| spelling | Carbohydrate metabolism in cultured cells ed. by Michael J. Morgan New York u.a. Plenum Pr. 1986 XXI, 514 S. txt rdacontent n rdamedia nc rdacarrier Cellules - Culture Cellules - Métabolisme Glucides - Métabolisme Carbohydrate Metabolism Carbohydrates Metabolism Cell culture Cell metabolism Cells, Cultured metabolism Primärstoffwechsel (DE-588)4175700-2 gnd rswk-swf Kohlenhydratstoffwechsel (DE-588)4031656-7 gnd rswk-swf Zellkultur (DE-588)4067547-6 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Zellkultur (DE-588)4067547-6 s Kohlenhydratstoffwechsel (DE-588)4031656-7 s DE-604 Primärstoffwechsel (DE-588)4175700-2 s Morgan, Michael J. Sonstige oth HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=000382560&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Carbohydrate metabolism in cultured cells Cellules - Culture Cellules - Métabolisme Glucides - Métabolisme Carbohydrate Metabolism Carbohydrates Metabolism Cell culture Cell metabolism Cells, Cultured metabolism Primärstoffwechsel (DE-588)4175700-2 gnd Kohlenhydratstoffwechsel (DE-588)4031656-7 gnd Zellkultur (DE-588)4067547-6 gnd |
| subject_GND | (DE-588)4175700-2 (DE-588)4031656-7 (DE-588)4067547-6 (DE-588)4143413-4 |
| title | Carbohydrate metabolism in cultured cells |
| title_auth | Carbohydrate metabolism in cultured cells |
| title_exact_search | Carbohydrate metabolism in cultured cells |
| title_full | Carbohydrate metabolism in cultured cells ed. by Michael J. Morgan |
| title_fullStr | Carbohydrate metabolism in cultured cells ed. by Michael J. Morgan |
| title_full_unstemmed | Carbohydrate metabolism in cultured cells ed. by Michael J. Morgan |
| title_short | Carbohydrate metabolism in cultured cells |
| title_sort | carbohydrate metabolism in cultured cells |
| topic | Cellules - Culture Cellules - Métabolisme Glucides - Métabolisme Carbohydrate Metabolism Carbohydrates Metabolism Cell culture Cell metabolism Cells, Cultured metabolism Primärstoffwechsel (DE-588)4175700-2 gnd Kohlenhydratstoffwechsel (DE-588)4031656-7 gnd Zellkultur (DE-588)4067547-6 gnd |
| topic_facet | Cellules - Culture Cellules - Métabolisme Glucides - Métabolisme Carbohydrate Metabolism Carbohydrates Metabolism Cell culture Cell metabolism Cells, Cultured metabolism Primärstoffwechsel Kohlenhydratstoffwechsel Zellkultur Aufsatzsammlung |
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