Ionic channels in cells and model systems:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York [u.a.]
Plenum Press
1986
|
| Schriftenreihe: | Series of the Centro de Estudios Científicos de Santiago
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XXIII, 437 S. |
| ISBN: | 0306421941 |
Internformat
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| 650 | 4 | |a Cell Membrane | |
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Datensatz im Suchindex
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|---|---|
| adam_text | Ionic Channels in
Cells and Model
Systems
Edited by
Ramon Latorre
Centro de Estudios Cientfficos de Santiago
and Facultad de Ciencias
Universidad de Chile
Santiago, Chile
PLENUM PRESS • NEW YORK AND LONDON
Contents
Introduction 1
I Methodologies
Chapter 1
Kinetic Models and Channel Fluctuations
Osvaldo Alvarez
1 Introduction 5
2 Two-State Channel 5
3 Two Two-State Channels : 11
4 Three-State Channel with Three Conductances 13
5 Three-State Channel with Only Two Conductances 13
References 15
Chapter 2
Single-Channel Currents and Postsynaptic Drug Actions
Harold- Lecar
1 Introduction 17
2 Channel Gating as a Stochastic Process 18
xv
xvi Contents
3 Postsynaptic Channels in the Presence of Drugs 24
4 Reconstructing the Postsynaptic Current 30
5 Macroscopic and Molecular Consequences 33
References 34
Chapter 3
Voltage-Dependent Gating: Gating Current Measurement
and Interpretation
Francisco Bezanilla
1 Introduction 37
2 Voltage Gating 37
3 Gating Current Is a Capacitive Current 38
4 Measurement of Gating Currents 39
5 Gating of the Sodium Channel 45
References 51
Chapter 4
Characterizing the Electrical Behavior of an Open Channel via the
Energy Profile for Ion Permeation: A Prototype Using a Fluctuating
Barrier Model for the Acetylcholine Receptor Channel
George Eisenman and John A Dani
1 Introduction 53
2 Theory 54
3 Confrontation with Experimental Data for the AChR Channel 63
4 Discussion 81
Appendix 83
References 85
Chapter 5
The Use of Specific Ligands to Study Sodium Channels in Muscle
Enrique Jaimovich
1 Introduction 89
2 Molecular Pharmacology of the Sodium Channel in Muscle 90
3 Sodium Channel in Cardiac Muscle: Are All Sodium Channels
Alike? 93
4 Surface and Tubular Sodium Channels in Skeletal Muscle 94
Contents xvii
5 Models for Sodium Channels in Muscle Membranes 96
References 98
Chapter 6
Isolation of Muscle Membranes Containing Functional Ionic Channels
Cecilia Hidalgo
1 Introduction 101
2 Excitation-Contraction Coupling 101
3 Ionic Channels and E-C Coupling 102
4 Isolation of Muscle Membranes 105
5 Concluding Remarks 120
References 120
Chapter 7
Methodologies to Study Channel-Mediated Ion Fluxes in
Membrane Vesicles
Ana Maria Garcia
1 Introduction 127
2 Channel-Mediated Tl+ Flux Measured by Fluorescence
Quenching 129
3 Channel-Mediated Ion Fluxes Measured by Light Scattering 136
References 139
Chapter 8
Optical Studies on Ionic Channels in Intact Vertebrate Nerve Terminals
Brian M Salzberg, Ana Lia Obaid, and Harold Gainer
1 Introduction 141
2 Equivalence of Optical and Electrical Measurements of
Membrane Potential 142
3 Optical Recording of Action Potentials from Nerve Terminals
of the Frog Xenopus 147
4 Properties of the Action Potential in the Nerve Terminals 151
5 Ionic Basis of the Depolarizing Phase of the Action Potential 154
6 Concluding Remarks 159
References 160
xviii Contents
Chapter 9
Optical Detection of ATP Release from Stimulated Endocrine Cells:
A Universal Marker ofExocytotic Secretion of Hormones
Eduardo Rojas, Rosa Maria Santos, Andres Stutzin, and
Harvey B Pollard
1 Introduction 163
2 Methodological Considerations 164
3 Acetylcholine-Induced ATP Release from Chromaffin Cells:
Calcium Dependence 168
4 Nicotinic Receptor Desensitization 169
5 Granular Nature of the Secreted ATP 170
6 ATP Release Evoked by Membrane Depolarization Is Mediated
by Activation of Voltage-Gated Calcium Channels 173
7 ATP Release from Collagenase-Isolated Islets of Langerhans 174
8 Conclusion 175
9 Summary 176
References 177
II Channels in Biological Membranes
Chapter 10
Mechanotransducing Ion Channels
Frederick Sachs
1 Introduction 181
2 Recording SA Channels 183
3 General Characteristics 184
4 Conductance Properties 185
5 Kinetic Properties 185
6 The Model 188
7 Comparing the Model to the Data 189
8 Future Prospects 192
References 192
Chapter 11
Ionic Channels in Plant Protoplasts
Nava Moran, Gerald Ehrenstein, Kunihiko Iwasa, Charles Bare, and
Charles Mischke
1 Introduction * 195
2 Some Methodological Considerations 197
3 Voltage-Dependent Channels Opened by Hyperpolarization 199
Contents xix
4 Channels Affected by TEA 201
5 Conclusions 203
References 204
Chapter 12
Channels in Kidney Epithelial Cells
Sandra Guggino
1 Introduction 207
2 Cell Culture 209
3 Patch-Clamp Methodology 209
4 Potassium Channel Characteristics 211
5 Channel Modulation 215
6 Conclusions 215
References 218
Chapter 13
Channels in Photoreceptors
Juan Bacigalupo
1 Introduction 221
2 Vertebrate Photoreceptors 223
3 Invertebrate Photoreceptors 225
References 232
Chapter 14
Inactivation of Calcium Currents in Muscle Fibers from Balanus
M Luxoro and V Nassar-Gentina
1 Introduction „ 235
2 Methodological Considerations * 236
3 Characteristics of Inward Currents 237
4 Mechanism of Inactivation 239
References 241
Chapter 15
Electrophysiological Studies in Endocrine Cells
Clay M Armstrong
1 Introduction 243
xx Contents
2 Whole-Cell Patch-Clamp Methodology 245
3 Cell Culture 247
4 Ionic Currents in GH3 Cells 247
5 Characteristics of Calcium Channels 249
6 Conclusions 252
References 254
III Ionic Channel Reconstitution
Chapter 16
Ion Channel Reconstitution: Why Bother?
Christopher Miller
1 Introduction and Background 257
2 Unexpected Surprises 263
3 Unconstrained Variables 264
4 Unrealized Hopes 267
References 269
Chapter 17
From Brain to Bilayer: Sodium Channels from Rat Neurons
Incorporated into Planar Lipid Membranes
Robert J French, Bruce K Krueger, and Jennings F Worley III
1 Perspectives and Background 273
2 Electrophysiology without Cells 277
3 A Closer Look at Batrachotoxin-Activated Sodium Channels in
Bilayer Membranes 280
4 Looking Ahead 287
References 288
Chapter 18
Ionic Channels in the Plasma Membrane of Sea Urchin Sperm
A Darszon, J Garcia-Soto, A Lievano, J A Sanchez, and
A D Islas-Trejo
1 Introduction 291
2 Are There Channels in Sea Urchin Sperm? 293
3 Reconstitution Studies with Isolated Sea Urchin Sperm Plasma
Membrane 294
Contents xxi
4 Channels in the Plasma Membrane of Sea Urchin Sperm:
Implications for the Acrosome Reaction 300
5 Are There Receptors to the Egg Jelly in the Sea Urchin Sperm
Plasma Membranes? 300
6 Perspectives 302
References 302
Chapter 19
Characterization of Large-Unitary-Conductance Calcium-Activated
Potassium Channels in Planar Lipid Bilayers
Daniel Wolff, Cecilia Vergara, Ximena Cecchi, and
Ramon Latorre
1 Introduction 307
2 Channel Gating 307
3 Channel Conductance and Selectivity 311
4 Conductance of the Calcium-Activated K+ Channels 312
5 Selectivity of the Ca-K Channels 313
6 Blockade of the Ca-K Channels 314
7 Conclusions 320
References 321
IV Ionic Channel Modulation
Chapter 20
Metabolic Regulation of Ion Channels
Irwin B Levitan
1 Introduction 325
2 Second Messengers 327
3 Protein Phosphorylation 329
4 Summary 331
References 332
Chapter 21
The Cell-to-Cell Membrane Channel: Its Regulation by
Cellular Phosphorylation
Werner R Loewenstein
1 Introduction 335
xxii Contents
2 The Cell-to-Cell Channels Are Up-Regulated by
cAMP-Dependent Phosphorylation 338
3 The Cell-to-Cell Channels are Down-Regulated by Tyrosine
Phosphorylation 344
References 349
Chapter 22
The B-Cell Bursting Pattern and Intracellular Calcium
Illani Atwater and John Rinzel
1 Introduction 353
2 Roleof [Ca2+];: Dependence on Glucose 354
3 A Biophysical/Mathematical Model 356
4 Burst Frequency Depends on the Ratio
[free Ca2+L/[total Ca]j 359
5 Summary 361
References 361
Chapter 23
Neurotrophic Effects of in Vitro Innervation of Cultured Muscle Cells
Modulation of Co2*-Activated K+ Conductances
Benjamin A Suarez-Isla and Stanley I Rapoport
1 Introduction 363
2 Methodological Considerations 364
3 Innervation and Muscle Cell Electrical Activity 367
4 Conclusions 380
References 381
V Ionic Channel Structure, Functions, and Models
Chapter 24
Correlation of the Molecular Structure with Functional Properties of
the Acetylcholine Receptor Protein
Francisco J Barrantes
1 Introduction 385
2 The AChR Macromolecule 386
3 Arrangement of Subunits in the AChR Macromolecule 387
4 The AChR Primary Structure, cDNA Recombinant Techniques,
and Modeling Receptor Structure 389
Contents xxiii
5 Immunochemistry of AChR and the Testing of Models 392
6 Voltage-Gated and Agonist-Gated Channels: A Comparison 392
7 Dynamics of AChR and Lipids in the Membrane 393
8 Acetylcholine-Receptor-Controlled Channel Properties 395
References 397
Chapter 25
Amiloride-Sensitive Epithelial Sodium Channels
Dale J Benos
1 Introduction 401
2 Amiloride-Sensitive Na+ Transport Processes 403
3 Characterization of Amiloride-Sensitive Na+ Channels in
Intact Epithelia 405
4 Incorporation of Amiloride-Sensitive Na+ Channels into
Planar Bilayers 412
5 Concluding Remarks 416
References 417
Chapter 26
A Channel Model for Development of the Fertilization Membrane in
Sea Urchin Eggs
Gerald Ehrenstein and Richard FitzHugh
1 Introduction 421
2 Processes Following Fertilization 421
3 Experimental Basis for Model 422
4 Description of Model 423
5 Equations of Model 425
6 Solutions of Model Equations 426
References „* 429
Index 431
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| spelling | Ionic channels in cells and model systems ed. by Ramon Latorre New York [u.a.] Plenum Press 1986 XXIII, 437 S. txt rdacontent n rdamedia nc rdacarrier Series of the Centro de Estudios Científicos de Santiago Cell Membranes cabt Ion Channels cabt Canaux ioniques Membrane cellulaire Modèles biologiques Biological models Cell Membrane Cell membranes Ion Channels Ion channels Models, Biological Biologisches System (DE-588)4122930-7 gnd rswk-swf Ionenkanal (DE-588)4138699-1 gnd rswk-swf Zelle (DE-588)4067537-3 gnd rswk-swf (DE-588)1071861417 Konferenzschrift 1984 Santiago de Chile gnd-content Ionenkanal (DE-588)4138699-1 s Zelle (DE-588)4067537-3 s DE-604 Biologisches System (DE-588)4122930-7 s Latorre, Ramón Sonstige oth HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=000377765&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Ionic channels in cells and model systems Cell Membranes cabt Ion Channels cabt Canaux ioniques Membrane cellulaire Modèles biologiques Biological models Cell Membrane Cell membranes Ion Channels Ion channels Models, Biological Biologisches System (DE-588)4122930-7 gnd Ionenkanal (DE-588)4138699-1 gnd Zelle (DE-588)4067537-3 gnd |
| subject_GND | (DE-588)4122930-7 (DE-588)4138699-1 (DE-588)4067537-3 (DE-588)1071861417 |
| title | Ionic channels in cells and model systems |
| title_auth | Ionic channels in cells and model systems |
| title_exact_search | Ionic channels in cells and model systems |
| title_full | Ionic channels in cells and model systems ed. by Ramon Latorre |
| title_fullStr | Ionic channels in cells and model systems ed. by Ramon Latorre |
| title_full_unstemmed | Ionic channels in cells and model systems ed. by Ramon Latorre |
| title_short | Ionic channels in cells and model systems |
| title_sort | ionic channels in cells and model systems |
| topic | Cell Membranes cabt Ion Channels cabt Canaux ioniques Membrane cellulaire Modèles biologiques Biological models Cell Membrane Cell membranes Ion Channels Ion channels Models, Biological Biologisches System (DE-588)4122930-7 gnd Ionenkanal (DE-588)4138699-1 gnd Zelle (DE-588)4067537-3 gnd |
| topic_facet | Cell Membranes Ion Channels Canaux ioniques Membrane cellulaire Modèles biologiques Biological models Cell Membrane Cell membranes Ion channels Models, Biological Biologisches System Ionenkanal Zelle Konferenzschrift 1984 Santiago de Chile |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=000377765&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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