Immunology in plant sciences:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Berlin [u.a.]
Springer
1986
|
| Schriftenreihe: | Modern methods of plant analysis
New series ; 4 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVIII, 263 S. Ill. |
| ISBN: | 3540168427 0387168427 |
Internformat
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| 245 | 1 | 0 | |a Immunology in plant sciences |c ed. by H. F. Linskens ... Contributors J. Brulfert ... |
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Datensatz im Suchindex
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| adam_text | ,-A TO IMMUNOLOGY IN PLANT SCIENCES EDITED BY H.F. LINSKENS AND J.F.
JACKSON CONTRIBUTORS J.BRULFERT M.-M.CORDONNIER D.ERNST H.GRISEBACH
M.G.HAHN A.KLEINHOFS T.M.KUO D.S.LUTHE D.W. ME CURDY P.MOESTA
K.R.NARAYANAN J.B. OHLROGGE L.H.PRATT R.J.ROBINS G.SCHMID Y.SHIMAZAKI
D.A.SOMERS K.C.VAUGHN J.VIDAL R.L.WARNER E.W.WEILER K.J.WILSON WITH 90
FIGURES SPRINGER-VERLAG BERLIN HEIDELBERG NEW YORK LONDON PARIS TOKYO
CONTENTS PLANT HORMONE IMMUNOASSAYS BASED ON MONOCLONAL AND POLYCLONAL
ANTIBODIES E.W. WEILER 1 INTRODUCTION 1 2 SYNTHESIS OF IMMUNOGENS 1 2.1
SYNTHESIS OF ABA CONJUGATES 2 2.1.1 PROCEDURE 1: SYNTHESIS OF
ABA-CCJ-BSA CONJUGATES (MODIFIED FROM WEILER 1980) 2 2.1.2 PROCEDURE 2:
SYNTHESIS OF ABA-C 4 -BSA CONJUGATES (ACCORDING TO WEILER 1980) 2 2.2
SYNTHESIS OF INDOLE-3-ACETIC ACID CONJUGATES 3 2.2.1 PROCEDURE 3:
SYNTHESIS OF IAA-BSA CONJUGATES VIA THE MIXED ANHYDRIDE 3 2.3 SYNTHESIS
OF GIBBERELLIN CONJUGATES 4 3 RADIO- AND ENZYME-LABELING OF PLANT
HORMONES 4 3.1 RADIOLABELED IMMUNOTRACERS 4 3.2 ENZYME-LABELED PLANT
HORMONES 5 3.2.1 PROCEDURE 4: SYNTHESIS OF ALKALINE PHOSPHATASE-LABELED
IAA (MODIFIED FROM WEILER ET AL. 1981) 5 3.2.2 PROCEDURE 5: COUPLING OF
ABA-C 4 -HYDRAZONES TO ALKALINE PHOSPHATASE 5 4 IMMUNOLOGICAL
PROCEDURES 6 4.1 IMMUNIZATION OF RABBITS 6 4.2 IMMUNIZATION OF MICE . .
. 7 5 ANTISERUM PROCESSING . . 7 6 PRODUCTION OF MONOCLONAL ANTIBODIES
8 6.1 GENERATION OF HYBRIDOMAS 8 6.2 MONOCLONAL ANTIBODY PRODUCTION AND
CHARACTERIZATION 8 7 RADIOIMMUNOASSAY PROCEDURES 9 7.1 PROCEDURE 6:
RADIOIMMUNOASSAY OF PLANT HORMONES 9 8 ENZYME IMMUNOASSAYS BASED ON
MONOCLONAL ANTIBODIES 10 8.1 PROCEDURE 7: ENZYME IMMUNOASSAY OF PLANT
HORMONES BASED ON MOUSE MONOCLONAL ANTIBODIES 11 9 PLANT IMMUNOANALYSIS
12 9.1 GENERAL REMARKS 12 9.2 VALIDATION OF IMMUNOASSAYS 12 9.3
PROCESSING OF PLANT MATERIAL 14 9.3.1 PROCEDURE8 . . * 14 9.3.2
PROCEDURE 9 15 REFERENCES 16 X CONTENTS RADIOIMMUNOASSAY AND GAS
CHROMATOGRAPHY/MASS SPECTROMETRY FOR CYTOKININ DETERMINATION D. ERNST
(WITH 14 FIGURES) 1 INTRODUCTION 18 2 THEORETICAL CONSIDERATIONS FOR
ANALYTICAL METHODS 18 3 RADIOIMMUNOASSAY 19 3.1 GENERAL PRINCIPLES 19
3.2 CYTOKININ ANTISERA 21 3.3 CYTOKININ DETERMINATION IN THE PRIMARY
EXTRACT 23 3.4 CYTOKININ DETERMINATION AFTER PRELIMINARY PURIFICATION OF
THE PRIMARY EXTRACT 24 3.5 METHODS 27 3.5.1 GENERAL ISOLATION PROTOCOL
27 3.5.2 SYNTHESIS OF CYTOKININ PROTEIN CONJUGATES 27 3.5.3 IMMUNIZATION
28 3.5.4 SYNTHESIS OF 3 H-CYTOKININ RIBOSIDE-DIALCOHOLS 28 3.5.5 RIA
PROTOCOL 29 4 GAS CHROMATOGRAPHY/MASS SPECTROMETRY 30 4.1 GENERAL
PRINCIPLES 30 4.2 IDENTIFICATION OF CYTOKININS 31 4.3 QUANTITATION OF
CYTOKININS 35 4.4 METHODS 40 4.4.1 GENERAL ISOLATION PROCEDURE AND GC/MS
40 4.4.2 SYNTHESIS OF DEUTERIUM-LABELED CYTOKININS 41 4.4.3 PRACTICAL
APPROACH 42 5 CONCLUDING REMARKS 42 REFERENCES 45 IMMUNODETECTION OF
PHYTOCHROME: IMMUNOCYTOCHEMISTRY, IMMUNOBLOTTING, AND IMMUNOQUANTITATION
L. H. PRATT, D. W. MCCURDY, Y. SHIMAZAKI, AND M.-M. CORDONNIER (WITH 12
FIGURES) 1 INTRODUCTION 50 1.1 ANTIBODY SPECIFICITY 50 1.2
IMMUNODETECTION METHODS 52 2 IMMUNOCYTOCHEMISTRY 52 2.1 RATIONALE . 52
2.2 METHOD 54 2.3 CONTROLS 56 2.4 EVALUATION OF METHOD 56 3
IMMUNOBLOTTING 58 3.1 RATIONALE 58 3.2 METHOD 59 3.3 CONTROLS 63 3.4
EVALUATION OF METHOD 64 4 IMMUNOQUANTITATION 66 4.1 RATIONALE 66
CONTENTS XI 4.2 METHOD 68 4.3 CONTROLS 70 4.4 EVALUATION OF METHOD 71 5
CONCLUDING REMARKS 73 REFERENCES 73 RADIOIMMUNOASSAY FOR A SOYBEAN
PHYTOALEXIN H. GRISEBACH, P. MOESTA, AND M. G. HAHN (WITH 5 FIGURES) 1
ISOLATION OF GLYCEOLLIN I 75 2 SYNTHESIS OF GLYCEOLLIN I-BSA CONJUGATE
76 2.1 PREPARATION OF P-AMINOHIPPURIC ACID SUBSTITUTED BSA 77 2.2
COUPLING OF GLYCEOLLIN I TO P-AMINOHIPPURIC ACID SUBSTITUTED BSA 77 3
GENERATION OF ANTISERA 77 4 PREPARATION OF THE [ 125 I]-GLYCEOLLIN I
TRACER 78 5 RADIOIMMUNOASSAY FOR GLYCEOLLIN I 79 6 SPECIFICITY OF THE
RADIOIMMUNOASSAY 80 7 APPLICATION OF THE RADIOIMMUNOASSAY FOR GLYCEOLLIN
I TO THE QUANTITATION OF PHYTOALEXINS IN INFECTED SOYBEAN TISSUE 81
REFERENCES 84 THE MEASUREMENT OF LOW-MOLECULAR-WEIGHT, NON-IMMUNOGENIC
COMPOUNDS BY IMMUNOASSAY R.J. ROBINS (WITH 11 FIGURES) 1 INTRODUCTION 86
1.1 THE ANALYTICAL ROLE OF THE IMMUNOASSAY 86 1.2 TYPES OF IMMUNOASSAY
87 1.3 AUTOMATION 92 2 DEVELOPMENT OF AN IMMUNOASSAY 92 2.1 SYNTHESIS OF
IMMUNOGEN . 92 2.1.1 METHODS OF CONJUGATION , . 94 2.1.1.1 CARBOXYLIC
ACID-DIRECTED REAGENTS 94 2.1.1.2 ALCOHOL-DIRECTED REAGENTS 94 2.1.1.3
PHENOLIC-DIRECTED REAGENTS 95 2.1.1.4 N-IN-AROMATIC-RING-DIRECTED
REAGENTS 95 2.1.1.5 AMINE-DIRECT REAGENTS 95 2.1.1.6 KETONE-DIRECTED
REAGENTS 96 2.1.1.7 SUGAR-DIRECTED REAGENTS 96 2.1.2 BRIDGE CONJUGATES
96 2.1.2.1 THE 6-AMINO-N-HEXANOIC ACID METHOD 97 2.1.2.2
ALCOHOL-DIRECTED REAGENTS 97 2.1.2.3 THE 4-AMINOHIPPURIC ACID METHOD 97
2.1.3 CHOICE OF PROTEIN 97 2.2 PRODUCTION OF ANTISERUM 98 2.2.1
IMMUNISATION PROCEDURE 98 2.3 SYNTHESIS OF LABELLED TRACERS 99 2.3.1
RADIOLABELLED TRACERS 99 XII CONTENTS 2.3.1.1 AROMATIC RING SUBSTITUTION
99 2.3.1.2 INCORPORATION AT THE POINT OF CONJUGATION . . . . 99 2.3.1.3
REDUCTION WITH SODIUM [ 3 H]-BOROHYDRIDE 100 2.3.1.4 [ 125
I]-INCORPORATION 100 2.3.2 ENZYME-LINKED TRACERS 100 2.4 ELISA PROCEDURE
101 2.4.1 MICROTITRATION PLATES 101 2.4.2 BUFFERS 101 2.4.3 INITIAL
TITRE MEASUREMENT 101 2.4.4 COATING CONCENTRATION 102 2.4.5 STANDARD
CURVE 103 2.4.6 SERUM DILUTION 104 2.4.7 SECOND ANTIBODY DILUTION 106
2.4.8 SECOND ANTIBODY DEVELOPMENT 106 2.4.8.1 ALKALINE PHOSPHATASE 106
2.4.8.2 PEROXIDASE 106 2.4.8.3 0-GALACTOSIDASE 106 2.4.9 ASSAY
VALIDATION 107 2.4.10 FUTURE DEVELOPMENTS 107 2.4.10.1 FLUORESCENT
ENZYME ASSAYS 107 2.4.10.2 THE BIOTIN-STREPTAVIDIN SYSTEM 108 2.4.10.3
THE ENZYMATIC RADIOIMMUNOASSAY 108 2.4.10.4 CHEMILUMINESCENT-LABELLED
ANTIBODIES 108 2.4.11 SPEED OF ASSAY 108 3 IMMMUNOASSAYS 109 3.1
ALKALOIDS 109 3.1.1 NICOTIANA ALKALOIDS 109 3.1.1.1 NICOTINE 109 3.1.1.2
COTININE 109 3.1.2 INDOLE ALKALOIDS OF CATHARANTHUS ROSEUS 110 3.1.2.1
VINCRISTINE AND VINBLASTINE 110 3.1.2.2 VINDOLINE 112 3.1.2.3 AJMALICINE
AND SERPENTINE 113 3.1.3 TROPANE ALKALOIDS 115 3.1.4 QUINOLINE ALKALOIDS
116 3.1.4.1 QUININE 117 3.1.4.2 QUINIDINE 117 3.1.5 PAPAVER ALKALOIDS
118 3.1.5.1 MORPHINE AND CODEINE 118 3.1.5.2 THE BENZYLISOQUINOLINE
PATHWAY 119 3.2 TERPENOIDS 120 3.2.1 LIMONIN 120 3.2.2 BRUCEANTIN 121
3.2.3 QUASSIN 122 3.2.4 IRIDOID GLUCOSIDES 122 3.3 FLAVONOIDS 124 3.3.1
NARINGIN 124 CONTENTS XIII 3.4 ANTHRONES 125 3.4.1 SENNOSIDES 125 3.5
STEROIDS 126 3.5.1 DIGITALIS CARDENOLIDES 126 3.5.1.1 DIGOXIN 127
3.5.1.2 DIGITOXIN 128 3.5.2 SOLANUM STEROIDAL GLYCOALKALOIDS 128 3.5.2.1
SOLANIDINE-DERIVED GLYCOALKALOIDS 130 3.5.2.2 SOLASODINE-DERIVED
GLYCOALKALOIDS 131 3.6 MISCELLANEOUS 132 3.6.1 PHYSIOLOGICALLY ACTIVE
METABOLITES 132 3.6.1.1 COLCHICINE 132 3.6.1.2 CHLORAMPHENICOL 132
3.6.1.3 PARAQUAT 133 3.6.2 CONTAMINATION OF FOOD AND FEEDSTUFFS 133
3.6.2.1 AFLATOXIN B T 133 3.6.2.2 STERIGMATOCYSTIN 134 3.6.2.3
OCHRATOXIN A 134 APPENDIX: SOURCES OF MATERIALS AND EQUIPMENT FOR ELISA
134 REFERENCES 137 RADIOIMMUNOASSAY AND WESTERN BLOT ANALYSIS OF ACYL
CARRIER PROTEIN ISOFORMS IN PLANTS T. M. KUO AND J. B. OHLROGGE (WITH 5
FIGURES) 1 INTRODUCTION 142 2 PRODUCTION OF ACP ANTIBODY 142 2.1
PURIFICATION OF SPINACH ACP ISOFORMS 142 2.2 IMMUNIZATION PROCEDURE 143
3 RADIOIMMUNOASSAY 144 3.1 PREPARATION OF RADIOLABELED ACP . 144 3.2
RADIOIMMUNOASSAY PROCEDURE . . 146 3.3 APPLICATIONS . 148 3.3.1 ACP
LEVELS DURING SOYBEAN SEED DEVELOPMENT 148 3.3.2 IMMUNO-CROSS-REACTIVITY
AMONG ACPS 148 4 IMMUNOLOGICAL ANALYSIS OF ACP ISOFORMS 150 4.1
IMMUNORELATIONSHIP OF SPINACH ACP ISOFORMS 150 4.2 WESTERN BLOT ANALYSIS
OF ACP ISOFORMS IN PLANT TISSUE 151 4.2.1 PREPARATION OF PLANT TISSUE
EXTRACTS 151 4.2.2 WESTERN BLOT ANALYSIS 152 5 CONCLUSION 153 REFERENCES
154 IMMUNOFLUORESCENT LABELLING OF ENZYMES G. SCHMID AND H. GRISEBACH
(WITH 9 FIGURES) 1 INTRODUCTION 156 2 GENERAL METHODOLOGY 157 XIV
CONTENTS 2.1 STAINING SEQUENCES 157 2.2 ANTIBODY PREPARATION 158 2.3
SPECIMEN PREPARATION 160 2.4 FLUORESCENCE MICROSCOPY 161 2.5 CONTROLS
.163 3 EXAMPLES FOR LOCALIZATION OF ENZYMES IN PLANT TISSUE BY
IMMUNOFLUORESCENT LABELING 163 3.1 IN SITU IMMUNOFLUORESCENT LABELING OF
RIBULOSEBISPHOSPHATE CARBOXYLASE IN LEAVES OF C 3 AND C 4 PLANTS 163 3.2
IN SITU IMMUNOFLUORESCENT LABELING OF A /?-GLUCOSIDASE FOR CONIFERIN AND
OF UDP-GLUCOSE: CONIFERYL ALCOHOL GLUCOSYLTRANSFERASE 165 3.2.1
LOCALIZATION OF YS-GLUCOSIDASE 165 3.2.2 LOCALIZATION OF
GLUCOSYLTRANSFERASE 167 REFERENCES 172 QUANTITATIVE IMMUNOCHEMISTRY OF
PLANT PHOSPHOENOLPYRUVATE CARBOXYLASES J. BRULFERT AND J. VIDAL (WITH 8
FIGURES) 1 PURIFICATION OF PEPC - PREPARATION OF IMMUNE SERA 175 1.1
EXTRACTION OF PEPC 176 1.2 PURIFICATION OF PEPC 176 1.3 PREPARATION OF
ANTISERA 177 1.4 PURIFICATION OF ANTISERA 177 1.4.1 FRACTIONATION BY
ION-EXCHANGE CHROMATOGRAPHY 177 1.4.2 AFFINITY CHROMATOGRAPHY UTILIZING
PROTEIN A IMMOBILIZED ON A SUPPORT 177 1.4.3 AFFINITY CHROMATOGRAPHY
UTILIZING ENZYME IMMOBILIZED ON AN ACTIVATED SUPPORT 178 2 QUANTITATIVE
IMMUNOPRECIPITATION IN GELS 179 3 IMMUNOTITRATION COUPLED TO ENZYME
ACTIVITY 179 3.1 MEASUREMENT OF PEPC RESIDUAL ACTIVITY IN THE
SUPERNATANT . . .179 3.2 MEASUREMENT OF PEPC ACTIVITY IN THE
IMMUNOPRECIPITATES . . . .181 3.3 APPLICATIONS 181 3.3.1 PEPC DURING
ESTABLISHMENT OF CAM AND C 4 -TYPE PHOTOSYNTHESIS 182 3.3.2
IMMUNOTITRATION OF PEPCS IN RELATION TO PLANT PHOTOSYNTHETHIC TYPES 184
3.3.3 MISCELLANEOUS 184 4 QUANTITATIVE IMMUNOPRECIPITATION IN EXTRACTS
184 4.1 HPLC TECHNIQUES 184 4.2 SDS-PAGE TECHNIQUES 185 5 IMMUNOAFFINITY
CHROMATOGRAPHY 186 6 CONCLUDING REMARKS 188 REFERENCES 188 CONTENTS XV
IMMUNOCHEMICAL METHODS FOR HIGHER PLANT NITRATE REDUCTASE A. KLEINHOFS,
K. R. NARAYANAN, D. A. SOMERS, T. M. KUO, AND R. L. WARNER (WITH 9
FIGURES) 1 INTRODUCTION 190 2 NITRATE REDUCTASE PURIFICATION 190 2.1
SEEDLING GROWTH CONDITIONS 190 2.2 EXTRACTION 191 2.3 AFFINITY
CHROMATOGRAPHY 192 2.4 ASSAYS 194 3 PRODUCTION OF NITRATE REDUCTASE
ANTISERUM 195 3.1 PREPARATION OF NITRATE REDUCTASE 195 3.2 IMMUNIZATION
195 3.3 SERUM COLLECTION AND ISOLATION 196 4 CHARACTERIZATION OF NITRATE
REDUCTASE ANTISERUM 196 4.1 ANTISERUM TITRATION 196 4.2 NR ANTISERUM
MONOSPECIFICITY 197 4.2.1 IMMUNODIFFUSION . 197 4.2.2 CROSSED
IMMUNOELECTROPHORESIS 198 5 APPLICATIONS 200 5.1 QUANTIFICATION OF
NITRATE REDUCTASE ANTIGEN 200 5.1.1 PROTECTION OF NITRATE REDUCTASE
INACTIVATION 200 5.1.2 ROCKET IMMUNOELECTROPHORESIS 201 5.1.3 OTHER
METHODS 203 5.2 WESTERN BLOT 203 5.2.1 NATIVE POLYACRYLAMIDE GEL
ELECTROPHORESIS 203 5.2.2 SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL
ELECTROPHORESIS (SDS-PAGE) 203 5.2.3 ELECTROPHORETIC TRANSFER OF
PROTEINS FROM POLYACRYLAMIDE GELS TO NITROCELLULOSE SHEETS 204 5.2.4
IMMUNODETECTION OF NITRATE REDUCTASE 205 5.3 IMMUNOPURIFICATION OF
NITRATE REDUCTASE , . . 207 5.3.1 IMMUNELINE ELECTROPHORESIS . 207 5.3.2
ANTIBODY COLUMN 208 6 SUMMARY 208 REFERENCES 209
IMMUNOLOGICAL-CYTOCHEMICAL LOCALIZATION OF CELL PRODUCTS IN PLANT TISSUE
CULTURE K.J. WILSON (WITH 10 FIGURES) 1 INTRODUCTION 212 2 THE LATICIFER
212 2.1 LATICIFER MORPHOLOGY AND DIFFERENTIATION IN THE ASCLEPIADACEAE .
213 2.2 METHODS OF PREPARING ANTI-LATEX ANTIBODIES 215 2.3 SECTION
PREPARATION 218 2.4 LATICIFER IDENTIFICATION IN ZYGOTIC MATERIAL 220 XVI
CONTENTS 3 THE TISSUE CULTURE SYSTEM FOR THE ASCLEPIADACEAE 222 3.1
CULTURE TECHNIQUES 222 3.2 EMBRYOID DIFFERENTIATION AND LATICIFER
IDENTIFICATION 223 4 FUTURE USES OF IMMUNOLOGICAL-CYTOCHEMICAL
TECHNIQUES IN TISSUE CULTURE 225 4.1 EMBRYOGENESIS AND PLANT HORMONES
225 4.2 EMBRYOGENESIS AND EMBRYOGENIC PROTEIN 226 4.3 CELL SORTING 227
REFERENCES 227 MEASUREMENT OF OAT GLOBULIN BY RADIOIMMUNOASSAY D. S.
LUTHE (WITH 5 FIGURES) 1 INTRODUCTION 231 1.1 THEORY OF THE RIA 231 1.2
OAT GLOBULIN 233 2 REQUIREMENTS FOR THE RADIOIMMUNOASSAY 233 2.1
PREPARATION OF PURE ANTIGEN 234 2.2 PREPARATION OF RADIOLABELED ANTIGEN
235 2.3 ANTIBODY PRODUCTION 237 2.4 SERUM AND IGG PREPARATION 238 2.5
PREPARATION OF OAT SEED EXTRACTS 239 3 DESIGNING THE RIA 239 3.1
SELECTING THE AMOUNT OF RADIOLABELED ANTIGEN 240 3.2 TITRATION OF THE
ANTIBODY 240 3.3 PRECIPITATION OF THE ANTIGEN/ANTIBODY COMPLEXES 240 3.4
CONDUCTING THE RIA 241 3.5 PREPARING THE STANDARD CURVE 242 3.6 DATA
TREATMENT 243 4 SUMMARY 244 REFERENCES 245 IMMUNOCYTOCHEMISTRY OF
CHLOROPLAST ANTIGENS K. C. VAUGHN (WITH 2 FIGURES) 1 INTRODUCTION 247 2
LIGHT MICROSCOPY 248 2.1 PROCEDURES FOR INDIRECT IMMUNOFLUORESCENCE 248
3 ELECTRON MICROSCOPY . 250 3.1 THE PEROXIDASE ANTI-PEROXIDASE (PAP)
METHODS (POST-EMBEDDING) 250 3.2 PEROXIDASE ANTI-PEROXIDASE PROCEDURE
251 3.3 COLLOIDAL GOLD 254 3.4 POST-EMBEDDING LABELING WITH COLLOIDAL
GOLD ON LOWICRYL-EMBEDDED TISSUE 254 4 CONCLUSIONS 257 REFERENCES 257
SUBJECT INDEX 259
|
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| discipline | Chemie / Pharmazie Biologie Chemie |
| format | Book |
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| genre | (DE-588)1071861417 Konferenzschrift 1985 Leeds gnd-content |
| genre_facet | Konferenzschrift 1985 Leeds |
| id | DE-604.BV000500316 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T15:14:55Z |
| institution | BVB |
| isbn | 3540168427 0387168427 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-000310136 |
| oclc_num | 13822051 |
| open_access_boolean | |
| owner | DE-12 DE-19 DE-BY-UBM DE-M49 DE-BY-TUM DE-703 DE-355 DE-BY-UBR DE-29T DE-824 DE-20 DE-83 DE-11 DE-188 |
| owner_facet | DE-12 DE-19 DE-BY-UBM DE-M49 DE-BY-TUM DE-703 DE-355 DE-BY-UBR DE-29T DE-824 DE-20 DE-83 DE-11 DE-188 |
| physical | XVIII, 263 S. Ill. |
| publishDate | 1986 |
| publishDateSearch | 1986 |
| publishDateSort | 1986 |
| publisher | Springer |
| record_format | marc |
| series | Modern methods of plant analysis |
| series2 | Modern methods of plant analysis : New series |
| spelling | Immunology in plant sciences ed. by H. F. Linskens ... Contributors J. Brulfert ... Berlin [u.a.] Springer 1986 XVIII, 263 S. Ill. txt rdacontent n rdamedia nc rdacarrier Modern methods of plant analysis : New series 4 Plantes - Analyse Plantes - Immunochimie Immunoassay Plant immunochemistry Plants Plants Analysis Immunologie (DE-588)4026637-0 gnd rswk-swf Phytochemie (DE-588)4174632-6 gnd rswk-swf Immunassay (DE-588)4113998-7 gnd rswk-swf Pflanzen (DE-588)4045539-7 gnd rswk-swf Pflanzenhormon (DE-588)4076052-2 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Pflanzenanalyse (DE-588)4174053-1 gnd rswk-swf (DE-588)1071861417 Konferenzschrift 1985 Leeds gnd-content Pflanzenhormon (DE-588)4076052-2 s Immunassay (DE-588)4113998-7 s DE-604 Phytochemie (DE-588)4174632-6 s Immunologie (DE-588)4026637-0 s Pflanzen (DE-588)4045539-7 s Pflanzenanalyse (DE-588)4174053-1 s Methode (DE-588)4038971-6 s Linskens, Hans F. 1921-2007 Sonstige (DE-588)139347585 oth Brulfert, J. Sonstige oth Modern methods of plant analysis New series ; 4 (DE-604)BV035361205 4 GBV Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=000310136&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Immunology in plant sciences Modern methods of plant analysis Plantes - Analyse Plantes - Immunochimie Immunoassay Plant immunochemistry Plants Plants Analysis Immunologie (DE-588)4026637-0 gnd Phytochemie (DE-588)4174632-6 gnd Immunassay (DE-588)4113998-7 gnd Pflanzen (DE-588)4045539-7 gnd Pflanzenhormon (DE-588)4076052-2 gnd Methode (DE-588)4038971-6 gnd Pflanzenanalyse (DE-588)4174053-1 gnd |
| subject_GND | (DE-588)4026637-0 (DE-588)4174632-6 (DE-588)4113998-7 (DE-588)4045539-7 (DE-588)4076052-2 (DE-588)4038971-6 (DE-588)4174053-1 (DE-588)1071861417 |
| title | Immunology in plant sciences |
| title_auth | Immunology in plant sciences |
| title_exact_search | Immunology in plant sciences |
| title_full | Immunology in plant sciences ed. by H. F. Linskens ... Contributors J. Brulfert ... |
| title_fullStr | Immunology in plant sciences ed. by H. F. Linskens ... Contributors J. Brulfert ... |
| title_full_unstemmed | Immunology in plant sciences ed. by H. F. Linskens ... Contributors J. Brulfert ... |
| title_short | Immunology in plant sciences |
| title_sort | immunology in plant sciences |
| topic | Plantes - Analyse Plantes - Immunochimie Immunoassay Plant immunochemistry Plants Plants Analysis Immunologie (DE-588)4026637-0 gnd Phytochemie (DE-588)4174632-6 gnd Immunassay (DE-588)4113998-7 gnd Pflanzen (DE-588)4045539-7 gnd Pflanzenhormon (DE-588)4076052-2 gnd Methode (DE-588)4038971-6 gnd Pflanzenanalyse (DE-588)4174053-1 gnd |
| topic_facet | Plantes - Analyse Plantes - Immunochimie Immunoassay Plant immunochemistry Plants Plants Analysis Immunologie Phytochemie Immunassay Pflanzen Pflanzenhormon Methode Pflanzenanalyse Konferenzschrift 1985 Leeds |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=000310136&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035361205 |
| work_keys_str_mv | AT linskenshansf immunologyinplantsciences AT brulfertj immunologyinplantsciences |