Expression of and antibody response to the herpes simplex virus specified major DNA-binding protein in acute and chronic herpes simplex virus infections:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Tampere
1985
|
| Schriftenreihe: | Tampereen Yliopisto: Acta Universitatis Tamperensis / A
193 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Zugl.: Tampere, Univ., Diss. |
| Beschreibung: | Getr. Zählung |
| ISBN: | 9514417976 |
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| 500 | |a Zugl.: Tampere, Univ., Diss. | ||
| 650 | 4 | |a Antigens | |
| 650 | 4 | |a Herpes simplex | |
| 650 | 4 | |a Herpesvirus diseases | |
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Datensatz im Suchindex
| _version_ | 1804114895991472128 |
|---|---|
| adam_text | 5
Contents
List of original communications 7
List of abbreviations 8 9
Introduction 10 11
Survey of literature
The agent 12 13
Clinical disease 13 16
Replication of HSV 16 19
Immunity to HSV infection 20 26
Cell virus interaction 26 29
Latency of HSV 29 33
Transformation by HSV 33 37
Natural history of cervical cancer,role of HSV 38 44
Aims of the present study 45
Material and Methods
Cell culture 46
Viruses and infection of the cells 46 47
Radioisotopes and chemicals 47
Antivirals and antisera 48
Labelling of the infected cell specified
polypeptides 48 49
Preparation of virus specified protein antigens 49 50
DNA cellulose column chromatography 50
Protein analysis 50 52
Immunological methods 52 55
Clinical material 55 56
Statistics 56
Results
1. Effect of PFA on HSV replication
1.1. Determination of effective doses of PFA 57
1.2. Enrichment of HSV specified DNA binding
proteins using PFA treated cells 58 62
2. Purification of the major HSV specified
DNA binding protein and analysis of its
biological properties
6
2.1. Detection of HSV specified DNA bindihg
proteins in different cell virus interactions 63
2.2. Purification and physicochemical
characteristics of the major HSV specified
DNA binding protein (ICSP 11/12) 63 65
2.3. Antibody response in rabbits against the
major HSV specified DNA binding protein 65 67
3. Serum antibodies to the HSV specified
protein antigens in patiens with acute HSV
infection or cervical neoplasia
3.1. Analysis of type common and type specific
antigen antibodies 67 68
3.2. ICSP 11/12 antibodies in patients
with acute HSV infection or cervical neoplasia 68 69
3.3. Other microbial antigen antibodies 70
4. In vitro latency of HSV
4.1. PFA treatment as an approach
to latent HSV infection, iri vitro 70 72
4.2. Replication of HSV in RAJI cells 72 74
Discussion
1. Synthesis of the major HSV specified
DNA binding protein (ICP 8, ICSP 11/12) 75 7 7
2. Physicochemical properties of the HSV
specified major DNA BP 77 80
3. ICSP 11/12 antibodies in cervical carcinoma
and control patients 80 85
4. Expression of ICSP 11/12 in non permissive
conditions 86 87
Summary 88 89
Acknowledgements 90 91
References 92 116
Original communications
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| spelling | Lehtinen, Matti Verfasser aut Expression of and antibody response to the herpes simplex virus specified major DNA-binding protein in acute and chronic herpes simplex virus infections Tampere 1985 Getr. Zählung txt rdacontent n rdamedia nc rdacarrier Tampereen Yliopisto: Acta Universitatis Tamperensis / A 193 Zugl.: Tampere, Univ., Diss. Antigens Herpes simplex Herpesvirus diseases Immune response Herpes simplex (DE-588)4137123-9 gnd rswk-swf Immungenetik (DE-588)4130579-6 gnd rswk-swf DNS-Bindungsproteine (DE-588)4150338-7 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Herpes simplex (DE-588)4137123-9 s Immungenetik (DE-588)4130579-6 s DNS-Bindungsproteine (DE-588)4150338-7 s DE-604 A Tampereen Yliopisto: Acta Universitatis Tamperensis 193 (DE-604)BV008009963 193 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=000269792&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Lehtinen, Matti Expression of and antibody response to the herpes simplex virus specified major DNA-binding protein in acute and chronic herpes simplex virus infections Antigens Herpes simplex Herpesvirus diseases Immune response Herpes simplex (DE-588)4137123-9 gnd Immungenetik (DE-588)4130579-6 gnd DNS-Bindungsproteine (DE-588)4150338-7 gnd |
| subject_GND | (DE-588)4137123-9 (DE-588)4130579-6 (DE-588)4150338-7 (DE-588)4143413-4 |
| title | Expression of and antibody response to the herpes simplex virus specified major DNA-binding protein in acute and chronic herpes simplex virus infections |
| title_auth | Expression of and antibody response to the herpes simplex virus specified major DNA-binding protein in acute and chronic herpes simplex virus infections |
| title_exact_search | Expression of and antibody response to the herpes simplex virus specified major DNA-binding protein in acute and chronic herpes simplex virus infections |
| title_full | Expression of and antibody response to the herpes simplex virus specified major DNA-binding protein in acute and chronic herpes simplex virus infections |
| title_fullStr | Expression of and antibody response to the herpes simplex virus specified major DNA-binding protein in acute and chronic herpes simplex virus infections |
| title_full_unstemmed | Expression of and antibody response to the herpes simplex virus specified major DNA-binding protein in acute and chronic herpes simplex virus infections |
| title_short | Expression of and antibody response to the herpes simplex virus specified major DNA-binding protein in acute and chronic herpes simplex virus infections |
| title_sort | expression of and antibody response to the herpes simplex virus specified major dna binding protein in acute and chronic herpes simplex virus infections |
| topic | Antigens Herpes simplex Herpesvirus diseases Immune response Herpes simplex (DE-588)4137123-9 gnd Immungenetik (DE-588)4130579-6 gnd DNS-Bindungsproteine (DE-588)4150338-7 gnd |
| topic_facet | Antigens Herpes simplex Herpesvirus diseases Immune response Immungenetik DNS-Bindungsproteine Aufsatzsammlung |
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