Synthetic biology :: a lab manual /
Synthetic Biology: A Lab Manual is the first manual for laboratory work in the new and rapidly expanding field of synthetic biology. Aimed at non-specialists, it details protocols central to synthetic biology in both education and research. In addition, it provides all the information that teachers...
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| Hauptverfasser: | , , |
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Hackensack, NJ :
World Scientific Publishing Company,
2014.
|
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Zusammenfassung: | Synthetic Biology: A Lab Manual is the first manual for laboratory work in the new and rapidly expanding field of synthetic biology. Aimed at non-specialists, it details protocols central to synthetic biology in both education and research. In addition, it provides all the information that teachers and students from high schools and tertiary institutions need for a colorful lab course in bacterial synthetic biology using chromoproteins and designer antisense RNAs. As a bonus, practical material is provided for students of the annual international Genetically Engineered Machine (iGEM) competition. The manual is based upon a highly successful course at Sweden's Uppsala University and is coauthored by one of the pioneers of synthetic biology and two bioengineering postgraduate students. An inspiring foreword is written by another pioneer in the field, Harvard's George Church: "Synthetic biology is to early recombinant DNA as a genome is to a gene. Is there anything that SynBio will not impact? There was no doubt that the field of SynBio needed 'A Lab Manual' such as the one that you now hold in your hands." The ebook version of this title will be available in April 2014 |
| Beschreibung: | 1 online resource |
| Bibliographie: | Includes bibliographical references and index. |
| ISBN: | 9789814579551 9814579556 9789814579544 9814579548 |
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| 505 | 0 | |a 1. Introduction. What is synthetic biology, exactly? The iGEM outbreak. A synthetic biology lab manual -- 2. Genes, chromoproteins and antisense RNAs. E. coli DNA: Chromosomes, plasmids and copy number. Coupling of transcription and translation in bacteria. Promoter and terminator for transcription. Ribosome binding site (RBS). Codon bias. Chromoproteins. Small regulatory RNAs (sRNAs) -- 3. Lab rooms and equipment. The physical lab spaces. Equipment -- 4. Safety is priority #1. Fires. Chemicals. Biological safety and disposal. Dangerous equipment -- 5. Lab course projects. Time and resources. Project overview and learning objectives. The lab notebook. Lab section 1. Preparation of chemical solutions and agar plates. Lab section 2. Coloring bacteria by adding a promoter to a chromoprotein gene. Lab section 3: Rational engineering of chromoprotein expression level. Lab section 4. Other experiments. The "dreaded" exam -- 6. Protocols. Introduction. Protocol 1. Preparation of solutions and agar plates. Protocol 2. Overnight cultures with antibiotics, and glycerol stocks. Protocol 3. BioBrick 3A assembly and gel analysis. Protocol 4. Agarose gel electrophoresis. Protocol 5. Preparation of competent E. coli cells using CaCl2. Protocol 6. Transformation of CaCl2-competent E. coli cells. Protocol 7. Bacterial re-streaking techniques. Protocol 8. Lysis of E. coli cells with lysozyme. Protocol 9. Polymerase chain reaction (PCR). Protocol 10. Inverse PCR mutagenesis. Protocol 11. Colony PCR. Protocol 12. Gibson assembly -- 7. Advanced methods. Flow cytometry and cell sorting. Recombination in plasmids and the chromosome. Electrocompetent cells -- 8. The International Genetically Engineered Machine (iGEM) Competition. How to start an iGEM team. Uppsala iGEM 2011 -- Show color with color. Uppsala iGEM 2012 -- Resistance is futile. Uppsala iGEM 2013 -- Lactonutritious -- it's delicious -- 9. Appendices. | |
| 520 | |a Synthetic Biology: A Lab Manual is the first manual for laboratory work in the new and rapidly expanding field of synthetic biology. Aimed at non-specialists, it details protocols central to synthetic biology in both education and research. In addition, it provides all the information that teachers and students from high schools and tertiary institutions need for a colorful lab course in bacterial synthetic biology using chromoproteins and designer antisense RNAs. As a bonus, practical material is provided for students of the annual international Genetically Engineered Machine (iGEM) competition. The manual is based upon a highly successful course at Sweden's Uppsala University and is coauthored by one of the pioneers of synthetic biology and two bioengineering postgraduate students. An inspiring foreword is written by another pioneer in the field, Harvard's George Church: "Synthetic biology is to early recombinant DNA as a genome is to a gene. Is there anything that SynBio will not impact? There was no doubt that the field of SynBio needed 'A Lab Manual' such as the one that you now hold in your hands." The ebook version of this title will be available in April 2014 | ||
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| author | Liljeruhm, Josefine Gullberg, Erik Forster, Anthony C. |
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| contents | 1. Introduction. What is synthetic biology, exactly? The iGEM outbreak. A synthetic biology lab manual -- 2. Genes, chromoproteins and antisense RNAs. E. coli DNA: Chromosomes, plasmids and copy number. Coupling of transcription and translation in bacteria. Promoter and terminator for transcription. Ribosome binding site (RBS). Codon bias. Chromoproteins. Small regulatory RNAs (sRNAs) -- 3. Lab rooms and equipment. The physical lab spaces. Equipment -- 4. Safety is priority #1. Fires. Chemicals. Biological safety and disposal. Dangerous equipment -- 5. Lab course projects. Time and resources. Project overview and learning objectives. The lab notebook. Lab section 1. Preparation of chemical solutions and agar plates. Lab section 2. Coloring bacteria by adding a promoter to a chromoprotein gene. Lab section 3: Rational engineering of chromoprotein expression level. Lab section 4. Other experiments. The "dreaded" exam -- 6. Protocols. Introduction. Protocol 1. Preparation of solutions and agar plates. Protocol 2. Overnight cultures with antibiotics, and glycerol stocks. Protocol 3. BioBrick 3A assembly and gel analysis. Protocol 4. Agarose gel electrophoresis. Protocol 5. Preparation of competent E. coli cells using CaCl2. Protocol 6. Transformation of CaCl2-competent E. coli cells. Protocol 7. Bacterial re-streaking techniques. Protocol 8. Lysis of E. coli cells with lysozyme. Protocol 9. Polymerase chain reaction (PCR). Protocol 10. Inverse PCR mutagenesis. Protocol 11. Colony PCR. Protocol 12. Gibson assembly -- 7. Advanced methods. Flow cytometry and cell sorting. Recombination in plasmids and the chromosome. Electrocompetent cells -- 8. The International Genetically Engineered Machine (iGEM) Competition. How to start an iGEM team. Uppsala iGEM 2011 -- Show color with color. Uppsala iGEM 2012 -- Resistance is futile. Uppsala iGEM 2013 -- Lactonutritious -- it's delicious -- 9. Appendices. |
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| dewey-search | 660.6 |
| dewey-sort | 3660.6 |
| dewey-tens | 660 - Chemical engineering |
| discipline | Chemie / Pharmazie |
| format | Electronic eBook |
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Introduction. What is synthetic biology, exactly? The iGEM outbreak. A synthetic biology lab manual -- 2. Genes, chromoproteins and antisense RNAs. E. coli DNA: Chromosomes, plasmids and copy number. Coupling of transcription and translation in bacteria. Promoter and terminator for transcription. Ribosome binding site (RBS). Codon bias. Chromoproteins. Small regulatory RNAs (sRNAs) -- 3. Lab rooms and equipment. The physical lab spaces. Equipment -- 4. Safety is priority #1. Fires. Chemicals. Biological safety and disposal. Dangerous equipment -- 5. Lab course projects. Time and resources. Project overview and learning objectives. The lab notebook. Lab section 1. Preparation of chemical solutions and agar plates. Lab section 2. Coloring bacteria by adding a promoter to a chromoprotein gene. Lab section 3: Rational engineering of chromoprotein expression level. Lab section 4. Other experiments. The "dreaded" exam -- 6. Protocols. Introduction. Protocol 1. Preparation of solutions and agar plates. Protocol 2. Overnight cultures with antibiotics, and glycerol stocks. Protocol 3. BioBrick 3A assembly and gel analysis. Protocol 4. Agarose gel electrophoresis. Protocol 5. Preparation of competent E. coli cells using CaCl2. Protocol 6. Transformation of CaCl2-competent E. coli cells. Protocol 7. Bacterial re-streaking techniques. Protocol 8. Lysis of E. coli cells with lysozyme. Protocol 9. Polymerase chain reaction (PCR). Protocol 10. Inverse PCR mutagenesis. Protocol 11. Colony PCR. Protocol 12. Gibson assembly -- 7. Advanced methods. Flow cytometry and cell sorting. Recombination in plasmids and the chromosome. Electrocompetent cells -- 8. The International Genetically Engineered Machine (iGEM) Competition. How to start an iGEM team. Uppsala iGEM 2011 -- Show color with color. Uppsala iGEM 2012 -- Resistance is futile. Uppsala iGEM 2013 -- Lactonutritious -- it's delicious -- 9. 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| indexdate | 2024-10-25T16:22:01Z |
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| publisher | World Scientific Publishing Company, |
| record_format | marc |
| spelling | Liljeruhm, Josefine, author. http://id.loc.gov/authorities/names/n2014019673 Synthetic biology : a lab manual / Josefine Liljeruhm, Erik Gullberg, Anthony C. Forster, Uppsala University, Sweden. Hackensack, NJ : World Scientific Publishing Company, 2014. 1 online resource text txt rdacontent computer c rdamedia online resource cr rdacarrier Includes bibliographical references and index. Online resource; title from PDF title page (Ebsco; viewed on May 21, 2014). 1. Introduction. What is synthetic biology, exactly? The iGEM outbreak. A synthetic biology lab manual -- 2. Genes, chromoproteins and antisense RNAs. E. coli DNA: Chromosomes, plasmids and copy number. Coupling of transcription and translation in bacteria. Promoter and terminator for transcription. Ribosome binding site (RBS). Codon bias. Chromoproteins. Small regulatory RNAs (sRNAs) -- 3. Lab rooms and equipment. The physical lab spaces. Equipment -- 4. Safety is priority #1. Fires. Chemicals. Biological safety and disposal. Dangerous equipment -- 5. Lab course projects. Time and resources. Project overview and learning objectives. The lab notebook. Lab section 1. Preparation of chemical solutions and agar plates. Lab section 2. Coloring bacteria by adding a promoter to a chromoprotein gene. Lab section 3: Rational engineering of chromoprotein expression level. Lab section 4. Other experiments. The "dreaded" exam -- 6. Protocols. Introduction. Protocol 1. Preparation of solutions and agar plates. Protocol 2. Overnight cultures with antibiotics, and glycerol stocks. Protocol 3. BioBrick 3A assembly and gel analysis. Protocol 4. Agarose gel electrophoresis. Protocol 5. Preparation of competent E. coli cells using CaCl2. Protocol 6. Transformation of CaCl2-competent E. coli cells. Protocol 7. Bacterial re-streaking techniques. Protocol 8. Lysis of E. coli cells with lysozyme. Protocol 9. Polymerase chain reaction (PCR). Protocol 10. Inverse PCR mutagenesis. Protocol 11. Colony PCR. Protocol 12. Gibson assembly -- 7. Advanced methods. Flow cytometry and cell sorting. Recombination in plasmids and the chromosome. Electrocompetent cells -- 8. The International Genetically Engineered Machine (iGEM) Competition. How to start an iGEM team. Uppsala iGEM 2011 -- Show color with color. Uppsala iGEM 2012 -- Resistance is futile. Uppsala iGEM 2013 -- Lactonutritious -- it's delicious -- 9. Appendices. Synthetic Biology: A Lab Manual is the first manual for laboratory work in the new and rapidly expanding field of synthetic biology. Aimed at non-specialists, it details protocols central to synthetic biology in both education and research. In addition, it provides all the information that teachers and students from high schools and tertiary institutions need for a colorful lab course in bacterial synthetic biology using chromoproteins and designer antisense RNAs. As a bonus, practical material is provided for students of the annual international Genetically Engineered Machine (iGEM) competition. The manual is based upon a highly successful course at Sweden's Uppsala University and is coauthored by one of the pioneers of synthetic biology and two bioengineering postgraduate students. An inspiring foreword is written by another pioneer in the field, Harvard's George Church: "Synthetic biology is to early recombinant DNA as a genome is to a gene. Is there anything that SynBio will not impact? There was no doubt that the field of SynBio needed 'A Lab Manual' such as the one that you now hold in your hands." The ebook version of this title will be available in April 2014 Synthetic biology Laboratory manuals. Biologie synthétique Manuels de laboratoire. SCIENCE Chemistry Industrial & Technical. bisacsh TECHNOLOGY & ENGINEERING Chemical & Biochemical. bisacsh Synthetic biology fast Laboratory Manual https://id.nlm.nih.gov/mesh/D020484 Laboratory manuals fast Laboratory manuals. lcgft http://id.loc.gov/authorities/genreForms/gf2014026120 Manuels de laboratoire. rvmgf Gullberg, Erik, author. http://id.loc.gov/authorities/names/n2014019674 Forster, Anthony C., author. http://id.loc.gov/authorities/names/n2014019676 has work: Synthetic biology (Text) https://id.oclc.org/worldcat/entity/E39PCFGtbVjvKf8WbqXDXCRPcd https://id.oclc.org/worldcat/ontology/hasWork Print version: Liljeruhm, Josefine. Synthetic Biology : A Lab Manual. Singapore : World Scientific Publishing Company, ©2014 9789814579544 FWS01 ZDB-4-EBA FWS_PDA_EBA https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=779667 Volltext CBO01 ZDB-4-EBA FWS_PDA_EBA https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=779667 Volltext |
| spellingShingle | Liljeruhm, Josefine Gullberg, Erik Forster, Anthony C. Synthetic biology : a lab manual / 1. Introduction. What is synthetic biology, exactly? The iGEM outbreak. A synthetic biology lab manual -- 2. Genes, chromoproteins and antisense RNAs. E. coli DNA: Chromosomes, plasmids and copy number. Coupling of transcription and translation in bacteria. Promoter and terminator for transcription. Ribosome binding site (RBS). Codon bias. Chromoproteins. Small regulatory RNAs (sRNAs) -- 3. Lab rooms and equipment. The physical lab spaces. Equipment -- 4. Safety is priority #1. Fires. Chemicals. Biological safety and disposal. Dangerous equipment -- 5. Lab course projects. Time and resources. Project overview and learning objectives. The lab notebook. Lab section 1. Preparation of chemical solutions and agar plates. Lab section 2. Coloring bacteria by adding a promoter to a chromoprotein gene. Lab section 3: Rational engineering of chromoprotein expression level. Lab section 4. Other experiments. The "dreaded" exam -- 6. Protocols. Introduction. Protocol 1. Preparation of solutions and agar plates. Protocol 2. Overnight cultures with antibiotics, and glycerol stocks. Protocol 3. BioBrick 3A assembly and gel analysis. Protocol 4. Agarose gel electrophoresis. Protocol 5. Preparation of competent E. coli cells using CaCl2. Protocol 6. Transformation of CaCl2-competent E. coli cells. Protocol 7. Bacterial re-streaking techniques. Protocol 8. Lysis of E. coli cells with lysozyme. Protocol 9. Polymerase chain reaction (PCR). Protocol 10. Inverse PCR mutagenesis. Protocol 11. Colony PCR. Protocol 12. Gibson assembly -- 7. Advanced methods. Flow cytometry and cell sorting. Recombination in plasmids and the chromosome. Electrocompetent cells -- 8. The International Genetically Engineered Machine (iGEM) Competition. How to start an iGEM team. Uppsala iGEM 2011 -- Show color with color. Uppsala iGEM 2012 -- Resistance is futile. Uppsala iGEM 2013 -- Lactonutritious -- it's delicious -- 9. Appendices. Synthetic biology Laboratory manuals. Biologie synthétique Manuels de laboratoire. SCIENCE Chemistry Industrial & Technical. bisacsh TECHNOLOGY & ENGINEERING Chemical & Biochemical. bisacsh Synthetic biology fast |
| subject_GND | https://id.nlm.nih.gov/mesh/D020484 http://id.loc.gov/authorities/genreForms/gf2014026120 |
| title | Synthetic biology : a lab manual / |
| title_auth | Synthetic biology : a lab manual / |
| title_exact_search | Synthetic biology : a lab manual / |
| title_full | Synthetic biology : a lab manual / Josefine Liljeruhm, Erik Gullberg, Anthony C. Forster, Uppsala University, Sweden. |
| title_fullStr | Synthetic biology : a lab manual / Josefine Liljeruhm, Erik Gullberg, Anthony C. Forster, Uppsala University, Sweden. |
| title_full_unstemmed | Synthetic biology : a lab manual / Josefine Liljeruhm, Erik Gullberg, Anthony C. Forster, Uppsala University, Sweden. |
| title_short | Synthetic biology : |
| title_sort | synthetic biology a lab manual |
| title_sub | a lab manual / |
| topic | Synthetic biology Laboratory manuals. Biologie synthétique Manuels de laboratoire. SCIENCE Chemistry Industrial & Technical. bisacsh TECHNOLOGY & ENGINEERING Chemical & Biochemical. bisacsh Synthetic biology fast |
| topic_facet | Synthetic biology Laboratory manuals. Biologie synthétique Manuels de laboratoire. SCIENCE Chemistry Industrial & Technical. TECHNOLOGY & ENGINEERING Chemical & Biochemical. Synthetic biology Laboratory Manual Laboratory manuals Laboratory manuals. Manuels de laboratoire. |
| url | https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=779667 |
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