Molecular biology techniques :: a classroom laboratory manual /
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hand...
Gespeichert in:
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| Weitere Verfasser: | , |
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Amsterdam ; Boston :
Elsevier /Academic Press,
©2012.
|
| Ausgabe: | 3rd ed. |
| Schriftenreihe: | ClinicalKey.
|
| Schlagworte: | |
| Online-Zugang: | Volltext Volltext |
| Zusammenfassung: | This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project" approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein-students can actually visualize positive clones following IPTG induction. *Cover basic concepts and techniques used in molecular biology research labs *Student-tested labs proven successful in a real classroom laboratories *Exercises simulate a cloning project that would be performed in a real research lab *"Project" approach to experiments gives students an overview of the entire process *Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions. |
| Beschreibung: | Rev. of: Manipulation and expression of recombinant DNA / Susan Carson, Dominique Robertson. 2nd ed. c2006. |
| Beschreibung: | 1 online resource (xxvi, 200 pages) : color illustrations. |
| Bibliographie: | Includes bibliographical references and index. |
| ISBN: | 9780123855459 0123855454 9786613310972 6613310972 |
Internformat
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| 245 | 1 | 0 | |a Molecular biology techniques : |b a classroom laboratory manual / |c Susan Carson, Heather Miller, D. Scott Witherow. |
| 250 | |a 3rd ed. | ||
| 260 | |a Amsterdam ; |a Boston : |b Elsevier /Academic Press, |c ©2012. | ||
| 300 | |a 1 online resource (xxvi, 200 pages) : |b color illustrations. | ||
| 336 | |a text |b txt |2 rdacontent | ||
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| 338 | |a online resource |b cr |2 rdacarrier | ||
| 347 | |a data file |2 rda | ||
| 490 | 1 | |a ClinicalKey | |
| 500 | |a Rev. of: Manipulation and expression of recombinant DNA / Susan Carson, Dominique Robertson. 2nd ed. c2006. | ||
| 504 | |a Includes bibliographical references and index. | ||
| 505 | 0 | |a Lab Session 1 Getting Oriented: Practicing with Micropipettes -- Lab Session 2 Purification and Digestion of Plasmid (Vector) DNA -- Lab Session 3 PCR Amplification of egfp and Completion of Vector Preparation -- Lab Session 4 Preparation of Insert DNA (egfp) PCR Product -- Lab Session 5 DNA Ligation and Transformation of Escherichia coli -- Lab Session 6 Colony Hybridization -- Lab Session 7 Characterization of Recombinant Clones: Part 1 -- Lab Session 8 Characterization of Recombinant Clones: Part 2 -- Lab Session 9 Characterization of Recombinant Clones: Part 3 -- Lab Session 10 Computational Analysis of DNA Sequence from a Positive Clone: Part 2 -- Lab Session 11 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 1 -- Lab Session 12 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 2 -- Lab Session 13 Extraction of Recombinant Protein from Escherichia coli Using a Glutathione Affinity Column -- Lab Session 14 Analysis of Purification Fractions -- Lab Session 15 Total RNA Purification -- Lab Session 16 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 1 -- Lab Session 17 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 2 -- Lab Session 18 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 1 -- Lab Session 19 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 2 -- Appendix 1 Equipment -- Appendix 2 Prep List -- Appendix 3 Preparation of Competent E. coli Cells -- Appendix 4 Pre-Lab Questions. | |
| 588 | 0 | |a Print version record. | |
| 520 | |a This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project" approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein-students can actually visualize positive clones following IPTG induction. *Cover basic concepts and techniques used in molecular biology research labs *Student-tested labs proven successful in a real classroom laboratories *Exercises simulate a cloning project that would be performed in a real research lab *"Project" approach to experiments gives students an overview of the entire process *Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions. | ||
| 546 | |a English. | ||
| 650 | 0 | |a Molecular biology |v Laboratory manuals. | |
| 650 | 0 | |a Molecular biology |x Technique |v Handbooks, manuals, etc. | |
| 650 | 0 | |a Recombinant DNA. |0 http://id.loc.gov/authorities/subjects/sh85111851 | |
| 650 | 0 | |a Protein engineering. |0 http://id.loc.gov/authorities/subjects/sh86007375 | |
| 650 | 1 | 2 | |a DNA, Recombinant |
| 650 | 2 | 2 | |a Molecular Biology |x methods |
| 650 | 2 | 2 | |a Protein Engineering |
| 650 | 6 | |a Biologie moléculaire |v Manuels de laboratoire. | |
| 650 | 6 | |a Biologie moléculaire |x Technique |v Guides, manuels, etc. | |
| 650 | 6 | |a ADN recombinant. | |
| 650 | 6 | |a Technologie des protéines. | |
| 650 | 7 | |a SCIENCE |x Life Sciences |x Molecular Biology. |2 bisacsh | |
| 650 | 7 | |a Recombinant DNA |2 fast | |
| 650 | 7 | |a Protein engineering |2 fast | |
| 650 | 7 | |a Molecular biology |2 fast | |
| 650 | 7 | |a Molecular biology |x Technique |2 fast | |
| 650 | 7 | |a Methode |2 gnd | |
| 650 | 7 | |a Molekularbiologie |2 gnd | |
| 650 | 7 | |a Methode |x (DE-588c)4038971-6. |2 swd | |
| 650 | 7 | |a Molekularbiologie |x (DE-588c)4039983-7. |2 swd | |
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| 655 | 2 | |a Laboratory Manual |0 https://id.nlm.nih.gov/mesh/D020484 | |
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| 700 | 1 | |a Miller, Heather B. |0 http://id.loc.gov/authorities/names/n2012181110 | |
| 700 | 1 | |a Witherow, D. Scott. |0 http://id.loc.gov/authorities/names/n2012181114 | |
| 776 | 0 | 8 | |i Print version: |a Carson, Sue (Susan). |t Molecular biology techniques. |b 3rd ed. |d Oxford ; Waltham, MA : Academic Press, 2012 |z 9780123855442 |w (DLC) 2012359021 |w (OCoLC)754167841 |
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| _version_ | 1816881777971560449 |
| adam_text | |
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| author | Carson, Sue (Susan) |
| author2 | Miller, Heather B. Witherow, D. Scott |
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| author_GND | http://id.loc.gov/authorities/names/no2006087798 http://id.loc.gov/authorities/names/n2012181110 http://id.loc.gov/authorities/names/n2012181114 |
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| contents | Lab Session 1 Getting Oriented: Practicing with Micropipettes -- Lab Session 2 Purification and Digestion of Plasmid (Vector) DNA -- Lab Session 3 PCR Amplification of egfp and Completion of Vector Preparation -- Lab Session 4 Preparation of Insert DNA (egfp) PCR Product -- Lab Session 5 DNA Ligation and Transformation of Escherichia coli -- Lab Session 6 Colony Hybridization -- Lab Session 7 Characterization of Recombinant Clones: Part 1 -- Lab Session 8 Characterization of Recombinant Clones: Part 2 -- Lab Session 9 Characterization of Recombinant Clones: Part 3 -- Lab Session 10 Computational Analysis of DNA Sequence from a Positive Clone: Part 2 -- Lab Session 11 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 1 -- Lab Session 12 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 2 -- Lab Session 13 Extraction of Recombinant Protein from Escherichia coli Using a Glutathione Affinity Column -- Lab Session 14 Analysis of Purification Fractions -- Lab Session 15 Total RNA Purification -- Lab Session 16 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 1 -- Lab Session 17 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 2 -- Lab Session 18 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 1 -- Lab Session 19 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 2 -- Appendix 1 Equipment -- Appendix 2 Prep List -- Appendix 3 Preparation of Competent E. coli Cells -- Appendix 4 Pre-Lab Questions. |
| ctrlnum | (OCoLC)763161431 |
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| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.8/078 |
| dewey-search | 572.8/078 |
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| dewey-tens | 570 - Biology |
| discipline | Biologie |
| edition | 3rd ed. |
| format | Electronic eBook |
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| genre | Handbook https://id.nlm.nih.gov/mesh/D020479 Laboratory Manual https://id.nlm.nih.gov/mesh/D020484 handbooks. aat manuals (instructional materials) aat Handbooks and manuals fast Laboratory manuals fast Handbooks and manuals. lcgft http://id.loc.gov/authorities/genreForms/gf2014026109 Laboratory manuals. lcgft http://id.loc.gov/authorities/genreForms/gf2014026120 Guides et manuels. rvmgf Manuels de laboratoire. rvmgf |
| genre_facet | Handbook Laboratory Manual handbooks. manuals (instructional materials) Handbooks and manuals Laboratory manuals Handbooks and manuals. Laboratory manuals. Guides et manuels. Manuels de laboratoire. |
| id | ZDB-4-EBA-ocn763161431 |
| illustrated | Illustrated |
| indexdate | 2024-11-27T13:18:07Z |
| institution | BVB |
| isbn | 9780123855459 0123855454 9786613310972 6613310972 |
| language | English |
| oclc_num | 763161431 |
| open_access_boolean | |
| owner | MAIN DE-863 DE-BY-FWS |
| owner_facet | MAIN DE-863 DE-BY-FWS |
| physical | 1 online resource (xxvi, 200 pages) : color illustrations. |
| psigel | ZDB-4-EBA |
| publishDate | 2012 |
| publishDateSearch | 2012 |
| publishDateSort | 2012 |
| publisher | Elsevier /Academic Press, |
| record_format | marc |
| series | ClinicalKey. |
| series2 | ClinicalKey |
| spelling | Carson, Sue (Susan) https://id.oclc.org/worldcat/entity/E39PCjJyhrG4vWDcgFJWv99DpX http://id.loc.gov/authorities/names/no2006087798 Molecular biology techniques : a classroom laboratory manual / Susan Carson, Heather Miller, D. Scott Witherow. 3rd ed. Amsterdam ; Boston : Elsevier /Academic Press, ©2012. 1 online resource (xxvi, 200 pages) : color illustrations. text txt rdacontent computer c rdamedia online resource cr rdacarrier data file rda ClinicalKey Rev. of: Manipulation and expression of recombinant DNA / Susan Carson, Dominique Robertson. 2nd ed. c2006. Includes bibliographical references and index. Lab Session 1 Getting Oriented: Practicing with Micropipettes -- Lab Session 2 Purification and Digestion of Plasmid (Vector) DNA -- Lab Session 3 PCR Amplification of egfp and Completion of Vector Preparation -- Lab Session 4 Preparation of Insert DNA (egfp) PCR Product -- Lab Session 5 DNA Ligation and Transformation of Escherichia coli -- Lab Session 6 Colony Hybridization -- Lab Session 7 Characterization of Recombinant Clones: Part 1 -- Lab Session 8 Characterization of Recombinant Clones: Part 2 -- Lab Session 9 Characterization of Recombinant Clones: Part 3 -- Lab Session 10 Computational Analysis of DNA Sequence from a Positive Clone: Part 2 -- Lab Session 11 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 1 -- Lab Session 12 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 2 -- Lab Session 13 Extraction of Recombinant Protein from Escherichia coli Using a Glutathione Affinity Column -- Lab Session 14 Analysis of Purification Fractions -- Lab Session 15 Total RNA Purification -- Lab Session 16 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 1 -- Lab Session 17 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 2 -- Lab Session 18 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 1 -- Lab Session 19 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 2 -- Appendix 1 Equipment -- Appendix 2 Prep List -- Appendix 3 Preparation of Competent E. coli Cells -- Appendix 4 Pre-Lab Questions. Print version record. This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project" approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein-students can actually visualize positive clones following IPTG induction. *Cover basic concepts and techniques used in molecular biology research labs *Student-tested labs proven successful in a real classroom laboratories *Exercises simulate a cloning project that would be performed in a real research lab *"Project" approach to experiments gives students an overview of the entire process *Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions. English. Molecular biology Laboratory manuals. Molecular biology Technique Handbooks, manuals, etc. Recombinant DNA. http://id.loc.gov/authorities/subjects/sh85111851 Protein engineering. http://id.loc.gov/authorities/subjects/sh86007375 DNA, Recombinant Molecular Biology methods Protein Engineering Biologie moléculaire Manuels de laboratoire. Biologie moléculaire Technique Guides, manuels, etc. ADN recombinant. Technologie des protéines. SCIENCE Life Sciences Molecular Biology. bisacsh Recombinant DNA fast Protein engineering fast Molecular biology fast Molecular biology Technique fast Methode gnd Molekularbiologie gnd Methode (DE-588c)4038971-6. swd Molekularbiologie (DE-588c)4039983-7. swd Handbook https://id.nlm.nih.gov/mesh/D020479 Laboratory Manual https://id.nlm.nih.gov/mesh/D020484 handbooks. aat manuals (instructional materials) aat Handbooks and manuals fast Laboratory manuals fast Handbooks and manuals. lcgft http://id.loc.gov/authorities/genreForms/gf2014026109 Laboratory manuals. lcgft http://id.loc.gov/authorities/genreForms/gf2014026120 Guides et manuels. rvmgf Manuels de laboratoire. rvmgf Miller, Heather B. http://id.loc.gov/authorities/names/n2012181110 Witherow, D. Scott. http://id.loc.gov/authorities/names/n2012181114 Print version: Carson, Sue (Susan). Molecular biology techniques. 3rd ed. Oxford ; Waltham, MA : Academic Press, 2012 9780123855442 (DLC) 2012359021 (OCoLC)754167841 ClinicalKey. FWS01 ZDB-4-EBA FWS_PDA_EBA https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=453823 Volltext FWS01 ZDB-4-EBA FWS_PDA_EBA https://www.sciencedirect.com/science/book/9780123855442 Volltext |
| spellingShingle | Carson, Sue (Susan) Molecular biology techniques : a classroom laboratory manual / ClinicalKey. Lab Session 1 Getting Oriented: Practicing with Micropipettes -- Lab Session 2 Purification and Digestion of Plasmid (Vector) DNA -- Lab Session 3 PCR Amplification of egfp and Completion of Vector Preparation -- Lab Session 4 Preparation of Insert DNA (egfp) PCR Product -- Lab Session 5 DNA Ligation and Transformation of Escherichia coli -- Lab Session 6 Colony Hybridization -- Lab Session 7 Characterization of Recombinant Clones: Part 1 -- Lab Session 8 Characterization of Recombinant Clones: Part 2 -- Lab Session 9 Characterization of Recombinant Clones: Part 3 -- Lab Session 10 Computational Analysis of DNA Sequence from a Positive Clone: Part 2 -- Lab Session 11 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 1 -- Lab Session 12 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 2 -- Lab Session 13 Extraction of Recombinant Protein from Escherichia coli Using a Glutathione Affinity Column -- Lab Session 14 Analysis of Purification Fractions -- Lab Session 15 Total RNA Purification -- Lab Session 16 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 1 -- Lab Session 17 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 2 -- Lab Session 18 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 1 -- Lab Session 19 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 2 -- Appendix 1 Equipment -- Appendix 2 Prep List -- Appendix 3 Preparation of Competent E. coli Cells -- Appendix 4 Pre-Lab Questions. Molecular biology Laboratory manuals. Molecular biology Technique Handbooks, manuals, etc. Recombinant DNA. http://id.loc.gov/authorities/subjects/sh85111851 Protein engineering. http://id.loc.gov/authorities/subjects/sh86007375 DNA, Recombinant Molecular Biology methods Protein Engineering Biologie moléculaire Manuels de laboratoire. Biologie moléculaire Technique Guides, manuels, etc. ADN recombinant. Technologie des protéines. SCIENCE Life Sciences Molecular Biology. bisacsh Recombinant DNA fast Protein engineering fast Molecular biology fast Molecular biology Technique fast Methode gnd Molekularbiologie gnd Methode (DE-588c)4038971-6. swd Molekularbiologie (DE-588c)4039983-7. swd |
| subject_GND | http://id.loc.gov/authorities/subjects/sh85111851 http://id.loc.gov/authorities/subjects/sh86007375 https://id.nlm.nih.gov/mesh/D020479 https://id.nlm.nih.gov/mesh/D020484 http://id.loc.gov/authorities/genreForms/gf2014026109 http://id.loc.gov/authorities/genreForms/gf2014026120 |
| title | Molecular biology techniques : a classroom laboratory manual / |
| title_auth | Molecular biology techniques : a classroom laboratory manual / |
| title_exact_search | Molecular biology techniques : a classroom laboratory manual / |
| title_full | Molecular biology techniques : a classroom laboratory manual / Susan Carson, Heather Miller, D. Scott Witherow. |
| title_fullStr | Molecular biology techniques : a classroom laboratory manual / Susan Carson, Heather Miller, D. Scott Witherow. |
| title_full_unstemmed | Molecular biology techniques : a classroom laboratory manual / Susan Carson, Heather Miller, D. Scott Witherow. |
| title_short | Molecular biology techniques : |
| title_sort | molecular biology techniques a classroom laboratory manual |
| title_sub | a classroom laboratory manual / |
| topic | Molecular biology Laboratory manuals. Molecular biology Technique Handbooks, manuals, etc. Recombinant DNA. http://id.loc.gov/authorities/subjects/sh85111851 Protein engineering. http://id.loc.gov/authorities/subjects/sh86007375 DNA, Recombinant Molecular Biology methods Protein Engineering Biologie moléculaire Manuels de laboratoire. Biologie moléculaire Technique Guides, manuels, etc. ADN recombinant. Technologie des protéines. SCIENCE Life Sciences Molecular Biology. bisacsh Recombinant DNA fast Protein engineering fast Molecular biology fast Molecular biology Technique fast Methode gnd Molekularbiologie gnd Methode (DE-588c)4038971-6. swd Molekularbiologie (DE-588c)4039983-7. swd |
| topic_facet | Molecular biology Laboratory manuals. Molecular biology Technique Handbooks, manuals, etc. Recombinant DNA. Protein engineering. DNA, Recombinant Molecular Biology methods Protein Engineering Biologie moléculaire Manuels de laboratoire. Biologie moléculaire Technique Guides, manuels, etc. ADN recombinant. Technologie des protéines. SCIENCE Life Sciences Molecular Biology. Recombinant DNA Protein engineering Molecular biology Molecular biology Technique Methode Molekularbiologie Methode (DE-588c)4038971-6. Molekularbiologie (DE-588c)4039983-7. Handbook Laboratory Manual handbooks. manuals (instructional materials) Handbooks and manuals Laboratory manuals Handbooks and manuals. Laboratory manuals. Guides et manuels. Manuels de laboratoire. |
| url | https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=453823 https://www.sciencedirect.com/science/book/9780123855442 |
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