Human stem cell manual :: a laboratory guide /
Stem cells are self-replicating and undifferentiated, meaning their function is not yet cell, tissue, or organ-specific. Due to the unique nature of these cells, research into their biology and function holds great promise for therapeutic applications through replacement or repair of diseased and da...
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| Weitere Verfasser: | , , |
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Amsterdam ; New York :
Academic Press,
2007.
|
| Schlagworte: | |
| Online-Zugang: | Volltext Volltext |
| Zusammenfassung: | Stem cells are self-replicating and undifferentiated, meaning their function is not yet cell, tissue, or organ-specific. Due to the unique nature of these cells, research into their biology and function holds great promise for therapeutic applications through replacement or repair of diseased and damaged cells. This reader-friendly manual provides a practical "hands on" guide to the culture of human embryonic and somatic stem cells. By presenting methods for embryonic and adult lines side-by-side, the authors lay out an elegant and unique path to understanding the science of stem cell practice. The authors begin with a broad-based introduction to the field, and also review legal and regulatory issues and patents. Each experimental strategy is presented with an historical introduction, detailed method, discussion of alternative methods, and common pitfalls. This lab guide for researchers also serves as a textbook for undergraduate and graduate students in laboratory courses |
| Beschreibung: | 1 online resource (xxiii, 461 pages) : illustrations (some color) |
| Bibliographie: | Includes bibliographical references and index. |
| ISBN: | 9780123704658 0123704650 9780080549880 0080549888 |
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| 260 | |a Amsterdam ; |a New York : |b Academic Press, |c 2007. | ||
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| 520 | |a Stem cells are self-replicating and undifferentiated, meaning their function is not yet cell, tissue, or organ-specific. Due to the unique nature of these cells, research into their biology and function holds great promise for therapeutic applications through replacement or repair of diseased and damaged cells. This reader-friendly manual provides a practical "hands on" guide to the culture of human embryonic and somatic stem cells. By presenting methods for embryonic and adult lines side-by-side, the authors lay out an elegant and unique path to understanding the science of stem cell practice. The authors begin with a broad-based introduction to the field, and also review legal and regulatory issues and patents. Each experimental strategy is presented with an historical introduction, detailed method, discussion of alternative methods, and common pitfalls. This lab guide for researchers also serves as a textbook for undergraduate and graduate students in laboratory courses | ||
| 505 | 0 | |a Foreword by James Battey -- Foreword by Jeannie Fontana -- Preface -- Part I: Basic methods: Maintenance of stem cell cultures -- 1. Human Embryonic Stem Cell Culture -- 2. Feeder layers and feeder-free culture -- 3. Mouse Embryonic Fibroblast Feeder Cells -- 4. Cryopreservation of hESC -- Part II: Basic methods: Characterization of stem cells -- 5. Classical Cytogenetics: Karyotyping -- 6. Spectral Karyotyping and Fluorescent in situ Hybridization -- 7. Using SNP Genotyping to Identify Cell Lines, Determine Genomic Abnormalities and Monitor DNA Methylation Patterns -- 8. FACS/Cell sorting -- 9. Fluorescence Immunocytochemical Analysis of Stem Cells -- 10. Characterization of Stem Cells Using RT-PCR -- 11. Gene Expression Profiling of Stem Cells by Microarray -- 12. Teratomas produced from Human Embryonic Stem Cells Xenografted into Immunodeficient Mice -- 13. Generation of HESC-Derived Teratomas -- Part III: Differentiation of stem cells -- 14. Embryoid Body Formation and Neuroepithelia Differentiation -- 15. Motor Neuron and Dopamine Neuron Differentiation -- 16. Oligodendrocyte Differentiation from hESC -- 17. Cardiac -- 18. Hematopoietic -- Part IV: Genetic manipulation of stem cells -- 19. Genetic Manipulation of hESC: Lentivirus Vectors -- 20. Methods developed for mouse ES cells -- Part V: Advanced methods -- 21. Derivation of human ES cells -- 22. Neural Stem Cell Culture -- 23. Transplantation of stem cells -- 24. In vitro fertilization -- 25. Functional characterization-neural cells -- Part VI: Practical issues in establishing a human ES cell laboratory -- 26. Setting Up a Facility for hESC Research -- Part VII: Essays on stem cell biology -- 27. Intellectual Property: Owning the Stem Cell -- 28. Ethical Concerns for Stem Cell Research -- 29. Guidelines for hESC Research Oversight (ESCRO) Committees. | |
| 504 | |a Includes bibliographical references and index. | ||
| 505 | 0 | |a Basic methods in stem cell culture -- Characterization of stem cells -- Differentiation of human embryonic cells -- Genetic manipulation of stem cells -- Advanced methods -- Setting up a laboratory -- Stem cells and society. | |
| 588 | 0 | |a Print version record. | |
| 650 | 0 | |a Stem cells |v Laboratory manuals. | |
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| 650 | 0 | |a Biology |x Research |x Methodology. | |
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| 650 | 2 | 2 | |a Biomedical Research |x methods |
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| 655 | 2 | |a Laboratory Manual | |
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| 700 | 1 | |a Schwartz, Philip H. |0 http://id.loc.gov/authorities/names/n2007183454 | |
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| author2 | Loring, Jeanne Frances, 1950- Wesselschmidt, Robin L. Schwartz, Philip H. |
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| contents | Foreword by James Battey -- Foreword by Jeannie Fontana -- Preface -- Part I: Basic methods: Maintenance of stem cell cultures -- 1. Human Embryonic Stem Cell Culture -- 2. Feeder layers and feeder-free culture -- 3. Mouse Embryonic Fibroblast Feeder Cells -- 4. Cryopreservation of hESC -- Part II: Basic methods: Characterization of stem cells -- 5. Classical Cytogenetics: Karyotyping -- 6. Spectral Karyotyping and Fluorescent in situ Hybridization -- 7. Using SNP Genotyping to Identify Cell Lines, Determine Genomic Abnormalities and Monitor DNA Methylation Patterns -- 8. FACS/Cell sorting -- 9. Fluorescence Immunocytochemical Analysis of Stem Cells -- 10. Characterization of Stem Cells Using RT-PCR -- 11. Gene Expression Profiling of Stem Cells by Microarray -- 12. Teratomas produced from Human Embryonic Stem Cells Xenografted into Immunodeficient Mice -- 13. Generation of HESC-Derived Teratomas -- Part III: Differentiation of stem cells -- 14. Embryoid Body Formation and Neuroepithelia Differentiation -- 15. Motor Neuron and Dopamine Neuron Differentiation -- 16. Oligodendrocyte Differentiation from hESC -- 17. Cardiac -- 18. Hematopoietic -- Part IV: Genetic manipulation of stem cells -- 19. Genetic Manipulation of hESC: Lentivirus Vectors -- 20. Methods developed for mouse ES cells -- Part V: Advanced methods -- 21. Derivation of human ES cells -- 22. Neural Stem Cell Culture -- 23. Transplantation of stem cells -- 24. In vitro fertilization -- 25. Functional characterization-neural cells -- Part VI: Practical issues in establishing a human ES cell laboratory -- 26. Setting Up a Facility for hESC Research -- Part VII: Essays on stem cell biology -- 27. Intellectual Property: Owning the Stem Cell -- 28. Ethical Concerns for Stem Cell Research -- 29. Guidelines for hESC Research Oversight (ESCRO) Committees. Basic methods in stem cell culture -- Characterization of stem cells -- Differentiation of human embryonic cells -- Genetic manipulation of stem cells -- Advanced methods -- Setting up a laboratory -- Stem cells and society. |
| ctrlnum | (OCoLC)162130510 |
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| dewey-search | 616/.02774 |
| dewey-sort | 3616 42774 |
| dewey-tens | 610 - Medicine and health |
| discipline | Medizin |
| format | Electronic eBook |
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Due to the unique nature of these cells, research into their biology and function holds great promise for therapeutic applications through replacement or repair of diseased and damaged cells. This reader-friendly manual provides a practical "hands on" guide to the culture of human embryonic and somatic stem cells. By presenting methods for embryonic and adult lines side-by-side, the authors lay out an elegant and unique path to understanding the science of stem cell practice. The authors begin with a broad-based introduction to the field, and also review legal and regulatory issues and patents. Each experimental strategy is presented with an historical introduction, detailed method, discussion of alternative methods, and common pitfalls. This lab guide for researchers also serves as a textbook for undergraduate and graduate students in laboratory courses</subfield></datafield><datafield tag="505" ind1="0" ind2=" "><subfield code="a">Foreword by James Battey -- Foreword by Jeannie Fontana -- Preface -- Part I: Basic methods: Maintenance of stem cell cultures -- 1. Human Embryonic Stem Cell Culture -- 2. Feeder layers and feeder-free culture -- 3. Mouse Embryonic Fibroblast Feeder Cells -- 4. Cryopreservation of hESC -- Part II: Basic methods: Characterization of stem cells -- 5. Classical Cytogenetics: Karyotyping -- 6. Spectral Karyotyping and Fluorescent in situ Hybridization -- 7. Using SNP Genotyping to Identify Cell Lines, Determine Genomic Abnormalities and Monitor DNA Methylation Patterns -- 8. FACS/Cell sorting -- 9. Fluorescence Immunocytochemical Analysis of Stem Cells -- 10. Characterization of Stem Cells Using RT-PCR -- 11. Gene Expression Profiling of Stem Cells by Microarray -- 12. Teratomas produced from Human Embryonic Stem Cells Xenografted into Immunodeficient Mice -- 13. Generation of HESC-Derived Teratomas -- Part III: Differentiation of stem cells -- 14. Embryoid Body Formation and Neuroepithelia Differentiation -- 15. Motor Neuron and Dopamine Neuron Differentiation -- 16. Oligodendrocyte Differentiation from hESC -- 17. Cardiac -- 18. Hematopoietic -- Part IV: Genetic manipulation of stem cells -- 19. Genetic Manipulation of hESC: Lentivirus Vectors -- 20. Methods developed for mouse ES cells -- Part V: Advanced methods -- 21. Derivation of human ES cells -- 22. Neural Stem Cell Culture -- 23. Transplantation of stem cells -- 24. In vitro fertilization -- 25. Functional characterization-neural cells -- Part VI: Practical issues in establishing a human ES cell laboratory -- 26. Setting Up a Facility for hESC Research -- Part VII: Essays on stem cell biology -- 27. Intellectual Property: Owning the Stem Cell -- 28. Ethical Concerns for Stem Cell Research -- 29. 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| genre | Laboratory Manual dissertations. aat Laboratory manuals fast Laboratory manuals. lcgft http://id.loc.gov/authorities/genreForms/gf2014026120 Academic theses. lcgft http://id.loc.gov/authorities/genreForms/gf2014026039 Manuels de laboratoire. rvmgf Thèses et écrits académiques. rvmgf |
| genre_facet | Laboratory Manual dissertations. Laboratory manuals Laboratory manuals. Academic theses. Manuels de laboratoire. Thèses et écrits académiques. |
| id | ZDB-4-EBA-ocn162130510 |
| illustrated | Illustrated |
| indexdate | 2024-11-27T13:16:06Z |
| institution | BVB |
| isbn | 9780123704658 0123704650 9780080549880 0080549888 |
| language | English |
| oclc_num | 162130510 |
| open_access_boolean | |
| owner | MAIN DE-863 DE-BY-FWS |
| owner_facet | MAIN DE-863 DE-BY-FWS |
| physical | 1 online resource (xxiii, 461 pages) : illustrations (some color) |
| psigel | ZDB-4-EBA |
| publishDate | 2007 |
| publishDateSearch | 2007 |
| publishDateSort | 2007 |
| publisher | Academic Press, |
| record_format | marc |
| spelling | Human stem cell manual : a laboratory guide / edited by Jeanne F. Loring, Robin L. Wesselschmidt, Philip H. Schwartz. Amsterdam ; New York : Academic Press, 2007. 1 online resource (xxiii, 461 pages) : illustrations (some color) text txt rdacontent computer c rdamedia online resource cr rdacarrier data file Stem cells are self-replicating and undifferentiated, meaning their function is not yet cell, tissue, or organ-specific. Due to the unique nature of these cells, research into their biology and function holds great promise for therapeutic applications through replacement or repair of diseased and damaged cells. This reader-friendly manual provides a practical "hands on" guide to the culture of human embryonic and somatic stem cells. By presenting methods for embryonic and adult lines side-by-side, the authors lay out an elegant and unique path to understanding the science of stem cell practice. The authors begin with a broad-based introduction to the field, and also review legal and regulatory issues and patents. Each experimental strategy is presented with an historical introduction, detailed method, discussion of alternative methods, and common pitfalls. This lab guide for researchers also serves as a textbook for undergraduate and graduate students in laboratory courses Foreword by James Battey -- Foreword by Jeannie Fontana -- Preface -- Part I: Basic methods: Maintenance of stem cell cultures -- 1. Human Embryonic Stem Cell Culture -- 2. Feeder layers and feeder-free culture -- 3. Mouse Embryonic Fibroblast Feeder Cells -- 4. Cryopreservation of hESC -- Part II: Basic methods: Characterization of stem cells -- 5. Classical Cytogenetics: Karyotyping -- 6. Spectral Karyotyping and Fluorescent in situ Hybridization -- 7. Using SNP Genotyping to Identify Cell Lines, Determine Genomic Abnormalities and Monitor DNA Methylation Patterns -- 8. FACS/Cell sorting -- 9. Fluorescence Immunocytochemical Analysis of Stem Cells -- 10. Characterization of Stem Cells Using RT-PCR -- 11. Gene Expression Profiling of Stem Cells by Microarray -- 12. Teratomas produced from Human Embryonic Stem Cells Xenografted into Immunodeficient Mice -- 13. Generation of HESC-Derived Teratomas -- Part III: Differentiation of stem cells -- 14. Embryoid Body Formation and Neuroepithelia Differentiation -- 15. Motor Neuron and Dopamine Neuron Differentiation -- 16. Oligodendrocyte Differentiation from hESC -- 17. Cardiac -- 18. Hematopoietic -- Part IV: Genetic manipulation of stem cells -- 19. Genetic Manipulation of hESC: Lentivirus Vectors -- 20. Methods developed for mouse ES cells -- Part V: Advanced methods -- 21. Derivation of human ES cells -- 22. Neural Stem Cell Culture -- 23. Transplantation of stem cells -- 24. In vitro fertilization -- 25. Functional characterization-neural cells -- Part VI: Practical issues in establishing a human ES cell laboratory -- 26. Setting Up a Facility for hESC Research -- Part VII: Essays on stem cell biology -- 27. Intellectual Property: Owning the Stem Cell -- 28. Ethical Concerns for Stem Cell Research -- 29. Guidelines for hESC Research Oversight (ESCRO) Committees. Includes bibliographical references and index. Basic methods in stem cell culture -- Characterization of stem cells -- Differentiation of human embryonic cells -- Genetic manipulation of stem cells -- Advanced methods -- Setting up a laboratory -- Stem cells and society. Print version record. Stem cells Laboratory manuals. Embryonic stem cells. http://id.loc.gov/authorities/subjects/sh92005532 Biology Research Methodology. Adult Stem Cells Biomedical Research methods Cellules souches Manuels de laboratoire. Cellules souches embryonnaires. Biologie Recherche Méthodologie. MEDICAL Research. bisacsh Biology Research Methodology fast Embryonic stem cells fast Stem cells fast Laboratory Manual dissertations. aat Laboratory manuals fast Laboratory manuals. lcgft http://id.loc.gov/authorities/genreForms/gf2014026120 Academic theses. lcgft http://id.loc.gov/authorities/genreForms/gf2014026039 Manuels de laboratoire. rvmgf Thèses et écrits académiques. rvmgf Loring, Jeanne Frances, 1950- https://id.oclc.org/worldcat/entity/E39PBJycJ846HfD3KkmGfQckjC http://id.loc.gov/authorities/names/n89630189 Wesselschmidt, Robin L. http://id.loc.gov/authorities/names/n2007183453 Schwartz, Philip H. http://id.loc.gov/authorities/names/n2007183454 Print version: Human stem cell manual. Amsterdam ; New York : Academic Press, 2007 9780123704658 0123704650 (OCoLC)150381365 FWS01 ZDB-4-EBA FWS_PDA_EBA https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=203125 Volltext FWS01 ZDB-4-EBA FWS_PDA_EBA https://www.sciencedirect.com/science/book/9780123704658 Volltext |
| spellingShingle | Human stem cell manual : a laboratory guide / Foreword by James Battey -- Foreword by Jeannie Fontana -- Preface -- Part I: Basic methods: Maintenance of stem cell cultures -- 1. Human Embryonic Stem Cell Culture -- 2. Feeder layers and feeder-free culture -- 3. Mouse Embryonic Fibroblast Feeder Cells -- 4. Cryopreservation of hESC -- Part II: Basic methods: Characterization of stem cells -- 5. Classical Cytogenetics: Karyotyping -- 6. Spectral Karyotyping and Fluorescent in situ Hybridization -- 7. Using SNP Genotyping to Identify Cell Lines, Determine Genomic Abnormalities and Monitor DNA Methylation Patterns -- 8. FACS/Cell sorting -- 9. Fluorescence Immunocytochemical Analysis of Stem Cells -- 10. Characterization of Stem Cells Using RT-PCR -- 11. Gene Expression Profiling of Stem Cells by Microarray -- 12. Teratomas produced from Human Embryonic Stem Cells Xenografted into Immunodeficient Mice -- 13. Generation of HESC-Derived Teratomas -- Part III: Differentiation of stem cells -- 14. Embryoid Body Formation and Neuroepithelia Differentiation -- 15. Motor Neuron and Dopamine Neuron Differentiation -- 16. Oligodendrocyte Differentiation from hESC -- 17. Cardiac -- 18. Hematopoietic -- Part IV: Genetic manipulation of stem cells -- 19. Genetic Manipulation of hESC: Lentivirus Vectors -- 20. Methods developed for mouse ES cells -- Part V: Advanced methods -- 21. Derivation of human ES cells -- 22. Neural Stem Cell Culture -- 23. Transplantation of stem cells -- 24. In vitro fertilization -- 25. Functional characterization-neural cells -- Part VI: Practical issues in establishing a human ES cell laboratory -- 26. Setting Up a Facility for hESC Research -- Part VII: Essays on stem cell biology -- 27. Intellectual Property: Owning the Stem Cell -- 28. Ethical Concerns for Stem Cell Research -- 29. Guidelines for hESC Research Oversight (ESCRO) Committees. Basic methods in stem cell culture -- Characterization of stem cells -- Differentiation of human embryonic cells -- Genetic manipulation of stem cells -- Advanced methods -- Setting up a laboratory -- Stem cells and society. Stem cells Laboratory manuals. Embryonic stem cells. http://id.loc.gov/authorities/subjects/sh92005532 Biology Research Methodology. Adult Stem Cells Biomedical Research methods Cellules souches Manuels de laboratoire. Cellules souches embryonnaires. Biologie Recherche Méthodologie. MEDICAL Research. bisacsh Biology Research Methodology fast Embryonic stem cells fast Stem cells fast |
| subject_GND | http://id.loc.gov/authorities/subjects/sh92005532 http://id.loc.gov/authorities/genreForms/gf2014026120 http://id.loc.gov/authorities/genreForms/gf2014026039 |
| title | Human stem cell manual : a laboratory guide / |
| title_auth | Human stem cell manual : a laboratory guide / |
| title_exact_search | Human stem cell manual : a laboratory guide / |
| title_full | Human stem cell manual : a laboratory guide / edited by Jeanne F. Loring, Robin L. Wesselschmidt, Philip H. Schwartz. |
| title_fullStr | Human stem cell manual : a laboratory guide / edited by Jeanne F. Loring, Robin L. Wesselschmidt, Philip H. Schwartz. |
| title_full_unstemmed | Human stem cell manual : a laboratory guide / edited by Jeanne F. Loring, Robin L. Wesselschmidt, Philip H. Schwartz. |
| title_short | Human stem cell manual : |
| title_sort | human stem cell manual a laboratory guide |
| title_sub | a laboratory guide / |
| topic | Stem cells Laboratory manuals. Embryonic stem cells. http://id.loc.gov/authorities/subjects/sh92005532 Biology Research Methodology. Adult Stem Cells Biomedical Research methods Cellules souches Manuels de laboratoire. Cellules souches embryonnaires. Biologie Recherche Méthodologie. MEDICAL Research. bisacsh Biology Research Methodology fast Embryonic stem cells fast Stem cells fast |
| topic_facet | Stem cells Laboratory manuals. Embryonic stem cells. Biology Research Methodology. Adult Stem Cells Biomedical Research methods Cellules souches Manuels de laboratoire. Cellules souches embryonnaires. Biologie Recherche Méthodologie. MEDICAL Research. Biology Research Methodology Embryonic stem cells Stem cells Laboratory Manual dissertations. Laboratory manuals Laboratory manuals. Academic theses. Manuels de laboratoire. Thèses et écrits académiques. |
| url | https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=203125 https://www.sciencedirect.com/science/book/9780123704658 |
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