Membrane protein purification and crystallization :: a practical guide /
This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical...
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| Weitere Verfasser: | , |
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Amsterdam ; Boston :
Academic Press,
©2003.
|
| Ausgabe: | 2nd ed. |
| Schlagworte: | |
| Online-Zugang: | Volltext Volltext |
| Zusammenfassung: | This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins. Key features * Provides general guidelines and strategies for isolation and crystallization of membrane proteins * Gives detailed protocols that have wide application, and low specialized equipment needs * Emphasizes recent progress in production and purification of recombinant membrane proteins, especially of histidine-tagged and other affinity-epitope-tagged proteins * Summarizes recent developments of Blue-Native PAGE, a high resolution separation technique, which is independent of the use of recombinant techniques, and is especially suited for proteomic analyses of membrane protein complexes * Gives detailed protocols for membrane protein crystallization, and describes the production and use of antibody fragments for high resolution crystallization * Presents a comprehensive guide to 2D-crystallization of membrane proteins. |
| Beschreibung: | 1 online resource (xviii, 316 pages) : illustrations (some color) |
| Format: | Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. |
| Bibliographie: | Includes bibliographical references and index. |
| ISBN: | 9780123617767 0123617766 9780080536170 0080536174 |
Internformat
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| 250 | |a 2nd ed. | ||
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| 520 | |a This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins. Key features * Provides general guidelines and strategies for isolation and crystallization of membrane proteins * Gives detailed protocols that have wide application, and low specialized equipment needs * Emphasizes recent progress in production and purification of recombinant membrane proteins, especially of histidine-tagged and other affinity-epitope-tagged proteins * Summarizes recent developments of Blue-Native PAGE, a high resolution separation technique, which is independent of the use of recombinant techniques, and is especially suited for proteomic analyses of membrane protein complexes * Gives detailed protocols for membrane protein crystallization, and describes the production and use of antibody fragments for high resolution crystallization * Presents a comprehensive guide to 2D-crystallization of membrane proteins. | ||
| 505 | 0 | |a I. Strategies and Techniques -- Purification Strategies for Membrane Proteins. -- Chromatographic Techniques and Basic Operations in Membrane Protein Purification. -- Production and purification of recombinant membrane proteins. -- SDS Electrophoresis Techniques. -- Blue-Native Electrophoresis. -- Preparative Isoelectric Focussing. -- Membrane protein crystallization. -- II. Discussion of Selected Isolation Protocols -- Lipid Dependent Inactivation and Reactivation of Bovine complex III. -- Purification of affinity-epitope tagged G-protein coupled receptors. -- Purification of NhaA, the Na+/H+ Antiporter of Escherichia coli for 3D or 2D -- Crystallization. -- Purification of the yeast cytochrome bc1 complex. -- -- III. Crystallization of Membrane Proteins -- Antibody fragment-mediated crystallization of membrane proteins. -- Crystallization of Wolinella succinogenes quinol:fumarate reductase. -- Ba3-type cytochrome-c oxidase from Thermus thermophilus: -- purification, crystallization, and crystal transformation. -- Two-dimensional crystallization of membrane proteins: a practical guide. -- In cubo crystallization of membrane proteins. | |
| 504 | |a Includes bibliographical references and index. | ||
| 588 | 0 | |a Print version record. | |
| 506 | |3 Use copy |f Restrictions unspecified |2 star |5 MiAaHDL | ||
| 533 | |a Electronic reproduction. |b [Place of publication not identified] : |c HathiTrust Digital Library, |d 2010. |5 MiAaHDL | ||
| 538 | |a Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. |u http://purl.oclc.org/DLF/benchrepro0212 |5 MiAaHDL | ||
| 583 | 1 | |a digitized |c 2010 |h HathiTrust Digital Library |l committed to preserve |2 pda |5 MiAaHDL | |
| 650 | 0 | |a Membrane proteins |x Purification. | |
| 650 | 7 | |a SCIENCE |x Life Sciences |x Anatomy & Physiology. |2 bisacsh | |
| 650 | 7 | |a SCIENCE |x Life Sciences |x Biochemistry. |2 bisacsh | |
| 650 | 7 | |a Membrane proteins |x Purification |2 fast | |
| 650 | 7 | |a Proteínas. |2 larpcal | |
| 650 | 7 | |a Membranas (biologia) |2 larpcal | |
| 650 | 7 | |a Bioquímica. |2 larpcal | |
| 700 | 1 | |a Jagow, Gebhard von. |0 http://id.loc.gov/authorities/names/n94027578 | |
| 700 | 1 | |a Schägger, Hermann. |0 http://id.loc.gov/authorities/names/n94027579 | |
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| author2 | Jagow, Gebhard von Schägger, Hermann |
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| author_facet | Jagow, Gebhard von Schägger, Hermann |
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| contents | I. Strategies and Techniques -- Purification Strategies for Membrane Proteins. -- Chromatographic Techniques and Basic Operations in Membrane Protein Purification. -- Production and purification of recombinant membrane proteins. -- SDS Electrophoresis Techniques. -- Blue-Native Electrophoresis. -- Preparative Isoelectric Focussing. -- Membrane protein crystallization. -- II. Discussion of Selected Isolation Protocols -- Lipid Dependent Inactivation and Reactivation of Bovine complex III. -- Purification of affinity-epitope tagged G-protein coupled receptors. -- Purification of NhaA, the Na+/H+ Antiporter of Escherichia coli for 3D or 2D -- Crystallization. -- Purification of the yeast cytochrome bc1 complex. -- -- III. Crystallization of Membrane Proteins -- Antibody fragment-mediated crystallization of membrane proteins. -- Crystallization of Wolinella succinogenes quinol:fumarate reductase. -- Ba3-type cytochrome-c oxidase from Thermus thermophilus: -- purification, crystallization, and crystal transformation. -- Two-dimensional crystallization of membrane proteins: a practical guide. -- In cubo crystallization of membrane proteins. |
| ctrlnum | (OCoLC)162129466 |
| dewey-full | 572/.696 |
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| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572/.696 |
| dewey-search | 572/.696 |
| dewey-sort | 3572 3696 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| edition | 2nd ed. |
| format | Electronic eBook |
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| id | ZDB-4-EBA-ocn162129466 |
| illustrated | Illustrated |
| indexdate | 2024-11-27T13:16:05Z |
| institution | BVB |
| isbn | 9780123617767 0123617766 9780080536170 0080536174 |
| language | English |
| oclc_num | 162129466 |
| open_access_boolean | |
| owner | MAIN DE-863 DE-BY-FWS |
| owner_facet | MAIN DE-863 DE-BY-FWS |
| physical | 1 online resource (xviii, 316 pages) : illustrations (some color) |
| psigel | ZDB-4-EBA |
| publishDate | 2003 |
| publishDateSearch | 2002 |
| publishDateSort | 2002 |
| publisher | Academic Press, |
| record_format | marc |
| spelling | Membrane protein purification and crystallization : a practical guide / edited by Carola Hunte and Gebhard von Jagow and Hermann Schagger. 2nd ed. Amsterdam ; Boston : Academic Press, ©2003. 1 online resource (xviii, 316 pages) : illustrations (some color) text txt rdacontent computer c rdamedia online resource cr rdacarrier This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins. Key features * Provides general guidelines and strategies for isolation and crystallization of membrane proteins * Gives detailed protocols that have wide application, and low specialized equipment needs * Emphasizes recent progress in production and purification of recombinant membrane proteins, especially of histidine-tagged and other affinity-epitope-tagged proteins * Summarizes recent developments of Blue-Native PAGE, a high resolution separation technique, which is independent of the use of recombinant techniques, and is especially suited for proteomic analyses of membrane protein complexes * Gives detailed protocols for membrane protein crystallization, and describes the production and use of antibody fragments for high resolution crystallization * Presents a comprehensive guide to 2D-crystallization of membrane proteins. I. Strategies and Techniques -- Purification Strategies for Membrane Proteins. -- Chromatographic Techniques and Basic Operations in Membrane Protein Purification. -- Production and purification of recombinant membrane proteins. -- SDS Electrophoresis Techniques. -- Blue-Native Electrophoresis. -- Preparative Isoelectric Focussing. -- Membrane protein crystallization. -- II. Discussion of Selected Isolation Protocols -- Lipid Dependent Inactivation and Reactivation of Bovine complex III. -- Purification of affinity-epitope tagged G-protein coupled receptors. -- Purification of NhaA, the Na+/H+ Antiporter of Escherichia coli for 3D or 2D -- Crystallization. -- Purification of the yeast cytochrome bc1 complex. -- -- III. Crystallization of Membrane Proteins -- Antibody fragment-mediated crystallization of membrane proteins. -- Crystallization of Wolinella succinogenes quinol:fumarate reductase. -- Ba3-type cytochrome-c oxidase from Thermus thermophilus: -- purification, crystallization, and crystal transformation. -- Two-dimensional crystallization of membrane proteins: a practical guide. -- In cubo crystallization of membrane proteins. Includes bibliographical references and index. Print version record. Use copy Restrictions unspecified star MiAaHDL Electronic reproduction. [Place of publication not identified] : HathiTrust Digital Library, 2010. MiAaHDL Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL Membrane proteins Purification. SCIENCE Life Sciences Anatomy & Physiology. bisacsh SCIENCE Life Sciences Biochemistry. bisacsh Membrane proteins Purification fast Proteínas. larpcal Membranas (biologia) larpcal Bioquímica. larpcal Jagow, Gebhard von. http://id.loc.gov/authorities/names/n94027578 Schägger, Hermann. http://id.loc.gov/authorities/names/n94027579 Print version: Membrane protein purification and crystallization. 2nd ed. Amsterdam ; Boston : Academic Press, ©2003 0123617766 9780123617767 (DLC) 2002100070 (OCoLC)54400634 FWS01 ZDB-4-EBA FWS_PDA_EBA https://www.sciencedirect.com/science/book/9780123617767 Volltext FWS01 ZDB-4-EBA FWS_PDA_EBA https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=205300 Volltext |
| spellingShingle | Membrane protein purification and crystallization : a practical guide / I. Strategies and Techniques -- Purification Strategies for Membrane Proteins. -- Chromatographic Techniques and Basic Operations in Membrane Protein Purification. -- Production and purification of recombinant membrane proteins. -- SDS Electrophoresis Techniques. -- Blue-Native Electrophoresis. -- Preparative Isoelectric Focussing. -- Membrane protein crystallization. -- II. Discussion of Selected Isolation Protocols -- Lipid Dependent Inactivation and Reactivation of Bovine complex III. -- Purification of affinity-epitope tagged G-protein coupled receptors. -- Purification of NhaA, the Na+/H+ Antiporter of Escherichia coli for 3D or 2D -- Crystallization. -- Purification of the yeast cytochrome bc1 complex. -- -- III. Crystallization of Membrane Proteins -- Antibody fragment-mediated crystallization of membrane proteins. -- Crystallization of Wolinella succinogenes quinol:fumarate reductase. -- Ba3-type cytochrome-c oxidase from Thermus thermophilus: -- purification, crystallization, and crystal transformation. -- Two-dimensional crystallization of membrane proteins: a practical guide. -- In cubo crystallization of membrane proteins. Membrane proteins Purification. SCIENCE Life Sciences Anatomy & Physiology. bisacsh SCIENCE Life Sciences Biochemistry. bisacsh Membrane proteins Purification fast Proteínas. larpcal Membranas (biologia) larpcal Bioquímica. larpcal |
| title | Membrane protein purification and crystallization : a practical guide / |
| title_auth | Membrane protein purification and crystallization : a practical guide / |
| title_exact_search | Membrane protein purification and crystallization : a practical guide / |
| title_full | Membrane protein purification and crystallization : a practical guide / edited by Carola Hunte and Gebhard von Jagow and Hermann Schagger. |
| title_fullStr | Membrane protein purification and crystallization : a practical guide / edited by Carola Hunte and Gebhard von Jagow and Hermann Schagger. |
| title_full_unstemmed | Membrane protein purification and crystallization : a practical guide / edited by Carola Hunte and Gebhard von Jagow and Hermann Schagger. |
| title_short | Membrane protein purification and crystallization : |
| title_sort | membrane protein purification and crystallization a practical guide |
| title_sub | a practical guide / |
| topic | Membrane proteins Purification. SCIENCE Life Sciences Anatomy & Physiology. bisacsh SCIENCE Life Sciences Biochemistry. bisacsh Membrane proteins Purification fast Proteínas. larpcal Membranas (biologia) larpcal Bioquímica. larpcal |
| topic_facet | Membrane proteins Purification. SCIENCE Life Sciences Anatomy & Physiology. SCIENCE Life Sciences Biochemistry. Membrane proteins Purification Proteínas. Membranas (biologia) Bioquímica. |
| url | https://www.sciencedirect.com/science/book/9780123617767 https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=205300 |
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