Gene expression systems :: using nature for the art of expression /
Gene Expression Systems: Using Nature for the Art of Expression offers detailed information on a wide variety of gene expression systems from an array of organisms. It describes several different types of expression systems including transient, stable, viral, and transgenic systems. Each chapter is...
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Format: | Elektronisch E-Book |
Sprache: | English |
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San Diego :
Academic Press,
©1999.
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Zusammenfassung: | Gene Expression Systems: Using Nature for the Art of Expression offers detailed information on a wide variety of gene expression systems from an array of organisms. It describes several different types of expression systems including transient, stable, viral, and transgenic systems. Each chapter is written by a leader in the field. The book includes timelines and examples for each expression system, and provides an overview of the future of recombinant protein expression. Key Features * Provides detailed information on expression systems * Covers a variety of promoters and host organisms enabling researchers to tailor protocols to their specific needs * Includes timelines and examples * Compares pros and cons of each method. |
Beschreibung: | 1 online resource (xii, 480 pages) : illustrations |
Format: | Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. |
Bibliographie: | Includes bibliographical references and index. |
ISBN: | 9780122538407 0122538404 9780080532356 0080532357 1281054186 9781281054180 9786611054182 6611054189 |
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245 | 0 | 0 | |a Gene expression systems : |b using nature for the art of expression / |c edited by Joseph M. Fernandez and James P. Hoeffler. |
260 | |a San Diego : |b Academic Press, |c ©1999. | ||
300 | |a 1 online resource (xii, 480 pages) : |b illustrations | ||
336 | |a text |b txt |2 rdacontent | ||
337 | |a computer |b c |2 rdamedia | ||
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520 | |a Gene Expression Systems: Using Nature for the Art of Expression offers detailed information on a wide variety of gene expression systems from an array of organisms. It describes several different types of expression systems including transient, stable, viral, and transgenic systems. Each chapter is written by a leader in the field. The book includes timelines and examples for each expression system, and provides an overview of the future of recombinant protein expression. Key Features * Provides detailed information on expression systems * Covers a variety of promoters and host organisms enabling researchers to tailor protocols to their specific needs * Includes timelines and examples * Compares pros and cons of each method. | ||
505 | 0 | |a Contributors. -- Introduction: The Art of Expression. -- Purpose of This Book. -- Selecting a Suitable Expression System: -- Considerations. -- Genomics and the Future of Protein Expression Systems, J.M. Fernandez and J.P. Hoeffler. -- Prokaryotic Expression Systems: -- Gene Expression Systems Based on Bacteriophage T7 RNA Polymearse, R. Durbin. -- Expression Vectors Employing the trc Promoter, J. Brosius. -- Bacillus Expression: A Gram Positive Model, E. Ferrari and B. Miller. -- araB Expression System in Escherichia coli, M. Better. -- Eukaryotic Expression Systems: -- Adenoviral Vectors for Protein Expression, D.J. von Seggern and G.R. Nemerow. -- Expression in the Methalotrophic Yeast Pichia pastoris, J.M. Cregg. -- Recombinant Protein Expression in Pichia methanolica, C.K. Raymond. -- Cytomegalovirus Promoter for Expression in Mammalian Cells, M.F. Stinski. -- Inducible Mammalian Expression Systems, M. Russell. -- Protein Expression in Mammalian Cells Using Sindbis Virus, R.P. Bennett. -- Expression in Insect Systems: -- Drosophila S2 System for Heterologous Gene Expression, R.B. Kirkpatrick and A. Shatzman. -- Baculovirus Expression Vector System, M. Galleno and A.J. Sick. -- Transgenic Expression: -- Recombinant Protein Expression in Transgenic Mice, R. Abbud and J.H. Nilson. -- Expression of Recombinant Proteins in the Milk of Transgenic Animals, H.M. Meade, Y. Echelard, C.A. Ziomek, M.W. Young, M. Harvey, E.S. Cole, S. Groet, T.E. Smith, and J.M. Curling. -- Recombinant Protein Expression in Plants, A.E. Voloudakis, Y. Yin, and R.N. Beachy. -- Index. | |
504 | |a Includes bibliographical references and index. | ||
505 | 0 | |a Gene expression systems based on bacteriophage T7 RNA polymerase -- Expression vectors employing the trc promoter -- Bacillus expression : a gram-positive model -- araB expression system in Escherichia coli -- Adenoviral vectors for protein expression -- Expression in the methylotrophic yeast Pichia pastoris -- Recombinant protein expression in Pichia methanolica -- Cytomegalovirus promoter for expression in mammalian cells -- Inducible mammalian expression systems -- Protein expression in mammalian cells using Sindbis virus -- Drosophila S2 system for heterologous gene expression -- Baculovirus expression vector system -- Recombinant protein expression in transgenic mice -- Expression of recombinant proteins in the milk of transgenic animals -- Recombinant protein expression in plants. | |
588 | 0 | |a Print version record. | |
506 | |3 Use copy |f Restrictions unspecified |2 star |5 MiAaHDL | ||
533 | |a Electronic reproduction. |b [Place of publication not identified] : |c HathiTrust Digital Library, |d 2010. |5 MiAaHDL | ||
538 | |a Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. |u http://purl.oclc.org/DLF/benchrepro0212 |5 MiAaHDL | ||
583 | 1 | |a digitized |c 2010 |h HathiTrust Digital Library |l committed to preserve |2 pda |5 MiAaHDL | |
546 | |a English. | ||
650 | 0 | |a Gene expression. |0 http://id.loc.gov/authorities/subjects/sh85053734 | |
650 | 0 | |a Prokaryotes |x Genetics. | |
650 | 0 | |a Insects |x Genetics. | |
650 | 0 | |a Transgenic animals |x Genetics. | |
650 | 0 | |a Eukaryotic cells. |0 http://id.loc.gov/authorities/subjects/sh85045546 | |
650 | 0 | |a Genetic regulation. |0 http://id.loc.gov/authorities/subjects/sh85053870 | |
650 | 2 | |a Prokaryotic Cells | |
650 | 2 | |a Gene Expression Regulation | |
650 | 2 | |a Gene Expression | |
650 | 2 | |a Animals, Genetically Modified |x genetics | |
650 | 2 | |a Eukaryotic Cells | |
650 | 6 | |a Expression génique. | |
650 | 6 | |a Procaryotes |x Génétique. | |
650 | 6 | |a Insectes |x Génétique. | |
650 | 6 | |a Animaux transgéniques |x Génétique. | |
650 | 6 | |a Cellules eucaryotes. | |
650 | 6 | |a Régulation génétique. | |
650 | 7 | |a SCIENCE |x Life Sciences |x Genetics & Genomics. |2 bisacsh | |
650 | 7 | |a Eukaryotic cells |2 fast | |
650 | 7 | |a Gene expression |2 fast | |
650 | 7 | |a Insects |x Genetics |2 fast | |
650 | 7 | |a Prokaryotes |x Genetics |2 fast | |
650 | 1 | 7 | |a Genexpressie. |2 gtt |
650 | 1 | 7 | |a Genregulatie. |2 gtt |
650 | 7 | |a Expression génique. |2 ram | |
650 | 7 | |a Procaryotes |x Génétique. |2 ram | |
650 | 7 | |a Insectes. |2 ram | |
650 | 7 | |a Animaux transgéniques. |2 ram | |
650 | 7 | |a Cellules eucaryotes. |2 ram | |
700 | 1 | |a Fernandez, Joseph M. |0 http://id.loc.gov/authorities/names/no99011783 | |
700 | 1 | |a Hoeffler, James P. |0 http://id.loc.gov/authorities/names/no99011781 | |
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Datensatz im Suchindex
DE-BY-FWS_katkey | ZDB-4-EBA-ocn162128726 |
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adam_text | |
any_adam_object | |
author2 | Fernandez, Joseph M. Hoeffler, James P. |
author2_role | |
author2_variant | j m f jm jmf j p h jp jph |
author_GND | http://id.loc.gov/authorities/names/no99011783 http://id.loc.gov/authorities/names/no99011781 |
author_facet | Fernandez, Joseph M. Hoeffler, James P. |
author_sort | Fernandez, Joseph M. |
building | Verbundindex |
bvnumber | localFWS |
callnumber-first | Q - Science |
callnumber-label | QH450 |
callnumber-raw | QH450 .G4626 1999eb |
callnumber-search | QH450 .G4626 1999eb |
callnumber-sort | QH 3450 G4626 41999EB |
callnumber-subject | QH - Natural History and Biology |
collection | ZDB-4-EBA |
contents | Contributors. -- Introduction: The Art of Expression. -- Purpose of This Book. -- Selecting a Suitable Expression System: -- Considerations. -- Genomics and the Future of Protein Expression Systems, J.M. Fernandez and J.P. Hoeffler. -- Prokaryotic Expression Systems: -- Gene Expression Systems Based on Bacteriophage T7 RNA Polymearse, R. Durbin. -- Expression Vectors Employing the trc Promoter, J. Brosius. -- Bacillus Expression: A Gram Positive Model, E. Ferrari and B. Miller. -- araB Expression System in Escherichia coli, M. Better. -- Eukaryotic Expression Systems: -- Adenoviral Vectors for Protein Expression, D.J. von Seggern and G.R. Nemerow. -- Expression in the Methalotrophic Yeast Pichia pastoris, J.M. Cregg. -- Recombinant Protein Expression in Pichia methanolica, C.K. Raymond. -- Cytomegalovirus Promoter for Expression in Mammalian Cells, M.F. Stinski. -- Inducible Mammalian Expression Systems, M. Russell. -- Protein Expression in Mammalian Cells Using Sindbis Virus, R.P. Bennett. -- Expression in Insect Systems: -- Drosophila S2 System for Heterologous Gene Expression, R.B. Kirkpatrick and A. Shatzman. -- Baculovirus Expression Vector System, M. Galleno and A.J. Sick. -- Transgenic Expression: -- Recombinant Protein Expression in Transgenic Mice, R. Abbud and J.H. Nilson. -- Expression of Recombinant Proteins in the Milk of Transgenic Animals, H.M. Meade, Y. Echelard, C.A. Ziomek, M.W. Young, M. Harvey, E.S. Cole, S. Groet, T.E. Smith, and J.M. Curling. -- Recombinant Protein Expression in Plants, A.E. Voloudakis, Y. Yin, and R.N. Beachy. -- Index. Gene expression systems based on bacteriophage T7 RNA polymerase -- Expression vectors employing the trc promoter -- Bacillus expression : a gram-positive model -- araB expression system in Escherichia coli -- Adenoviral vectors for protein expression -- Expression in the methylotrophic yeast Pichia pastoris -- Recombinant protein expression in Pichia methanolica -- Cytomegalovirus promoter for expression in mammalian cells -- Inducible mammalian expression systems -- Protein expression in mammalian cells using Sindbis virus -- Drosophila S2 system for heterologous gene expression -- Baculovirus expression vector system -- Recombinant protein expression in transgenic mice -- Expression of recombinant proteins in the milk of transgenic animals -- Recombinant protein expression in plants. |
ctrlnum | (OCoLC)162128726 |
dewey-full | 572.8/65 |
dewey-hundreds | 500 - Natural sciences and mathematics |
dewey-ones | 572 - Biochemistry |
dewey-raw | 572.8/65 |
dewey-search | 572.8/65 |
dewey-sort | 3572.8 265 |
dewey-tens | 570 - Biology |
discipline | Biologie |
format | Electronic eBook |
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Durbin. -- Expression Vectors Employing the trc Promoter, J. Brosius. -- Bacillus Expression: A Gram Positive Model, E. Ferrari and B. Miller. -- araB Expression System in Escherichia coli, M. Better. -- Eukaryotic Expression Systems: -- Adenoviral Vectors for Protein Expression, D.J. von Seggern and G.R. Nemerow. -- Expression in the Methalotrophic Yeast Pichia pastoris, J.M. Cregg. -- Recombinant Protein Expression in Pichia methanolica, C.K. Raymond. -- Cytomegalovirus Promoter for Expression in Mammalian Cells, M.F. Stinski. -- Inducible Mammalian Expression Systems, M. Russell. -- Protein Expression in Mammalian Cells Using Sindbis Virus, R.P. Bennett. -- Expression in Insect Systems: -- Drosophila S2 System for Heterologous Gene Expression, R.B. Kirkpatrick and A. Shatzman. -- Baculovirus Expression Vector System, M. Galleno and A.J. Sick. -- Transgenic Expression: -- Recombinant Protein Expression in Transgenic Mice, R. Abbud and J.H. 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id | ZDB-4-EBA-ocn162128726 |
illustrated | Illustrated |
indexdate | 2024-11-27T13:16:05Z |
institution | BVB |
isbn | 9780122538407 0122538404 9780080532356 0080532357 1281054186 9781281054180 9786611054182 6611054189 |
language | English |
oclc_num | 162128726 |
open_access_boolean | |
owner | MAIN DE-863 DE-BY-FWS |
owner_facet | MAIN DE-863 DE-BY-FWS |
physical | 1 online resource (xii, 480 pages) : illustrations |
psigel | ZDB-4-EBA |
publishDate | 1999 |
publishDateSearch | 1999 |
publishDateSort | 1999 |
publisher | Academic Press, |
record_format | marc |
spelling | Gene expression systems : using nature for the art of expression / edited by Joseph M. Fernandez and James P. Hoeffler. San Diego : Academic Press, ©1999. 1 online resource (xii, 480 pages) : illustrations text txt rdacontent computer c rdamedia online resource cr rdacarrier Gene Expression Systems: Using Nature for the Art of Expression offers detailed information on a wide variety of gene expression systems from an array of organisms. It describes several different types of expression systems including transient, stable, viral, and transgenic systems. Each chapter is written by a leader in the field. The book includes timelines and examples for each expression system, and provides an overview of the future of recombinant protein expression. Key Features * Provides detailed information on expression systems * Covers a variety of promoters and host organisms enabling researchers to tailor protocols to their specific needs * Includes timelines and examples * Compares pros and cons of each method. Contributors. -- Introduction: The Art of Expression. -- Purpose of This Book. -- Selecting a Suitable Expression System: -- Considerations. -- Genomics and the Future of Protein Expression Systems, J.M. Fernandez and J.P. Hoeffler. -- Prokaryotic Expression Systems: -- Gene Expression Systems Based on Bacteriophage T7 RNA Polymearse, R. Durbin. -- Expression Vectors Employing the trc Promoter, J. Brosius. -- Bacillus Expression: A Gram Positive Model, E. Ferrari and B. Miller. -- araB Expression System in Escherichia coli, M. Better. -- Eukaryotic Expression Systems: -- Adenoviral Vectors for Protein Expression, D.J. von Seggern and G.R. Nemerow. -- Expression in the Methalotrophic Yeast Pichia pastoris, J.M. Cregg. -- Recombinant Protein Expression in Pichia methanolica, C.K. Raymond. -- Cytomegalovirus Promoter for Expression in Mammalian Cells, M.F. Stinski. -- Inducible Mammalian Expression Systems, M. Russell. -- Protein Expression in Mammalian Cells Using Sindbis Virus, R.P. Bennett. -- Expression in Insect Systems: -- Drosophila S2 System for Heterologous Gene Expression, R.B. Kirkpatrick and A. Shatzman. -- Baculovirus Expression Vector System, M. Galleno and A.J. Sick. -- Transgenic Expression: -- Recombinant Protein Expression in Transgenic Mice, R. Abbud and J.H. Nilson. -- Expression of Recombinant Proteins in the Milk of Transgenic Animals, H.M. Meade, Y. Echelard, C.A. Ziomek, M.W. Young, M. Harvey, E.S. Cole, S. Groet, T.E. Smith, and J.M. Curling. -- Recombinant Protein Expression in Plants, A.E. Voloudakis, Y. Yin, and R.N. Beachy. -- Index. Includes bibliographical references and index. Gene expression systems based on bacteriophage T7 RNA polymerase -- Expression vectors employing the trc promoter -- Bacillus expression : a gram-positive model -- araB expression system in Escherichia coli -- Adenoviral vectors for protein expression -- Expression in the methylotrophic yeast Pichia pastoris -- Recombinant protein expression in Pichia methanolica -- Cytomegalovirus promoter for expression in mammalian cells -- Inducible mammalian expression systems -- Protein expression in mammalian cells using Sindbis virus -- Drosophila S2 system for heterologous gene expression -- Baculovirus expression vector system -- Recombinant protein expression in transgenic mice -- Expression of recombinant proteins in the milk of transgenic animals -- Recombinant protein expression in plants. Print version record. Use copy Restrictions unspecified star MiAaHDL Electronic reproduction. [Place of publication not identified] : HathiTrust Digital Library, 2010. MiAaHDL Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL English. Gene expression. http://id.loc.gov/authorities/subjects/sh85053734 Prokaryotes Genetics. Insects Genetics. Transgenic animals Genetics. Eukaryotic cells. http://id.loc.gov/authorities/subjects/sh85045546 Genetic regulation. http://id.loc.gov/authorities/subjects/sh85053870 Prokaryotic Cells Gene Expression Regulation Gene Expression Animals, Genetically Modified genetics Eukaryotic Cells Expression génique. Procaryotes Génétique. Insectes Génétique. Animaux transgéniques Génétique. Cellules eucaryotes. Régulation génétique. SCIENCE Life Sciences Genetics & Genomics. bisacsh Eukaryotic cells fast Gene expression fast Insects Genetics fast Prokaryotes Genetics fast Genexpressie. gtt Genregulatie. gtt Expression génique. ram Procaryotes Génétique. ram Insectes. ram Animaux transgéniques. ram Cellules eucaryotes. ram Fernandez, Joseph M. http://id.loc.gov/authorities/names/no99011783 Hoeffler, James P. http://id.loc.gov/authorities/names/no99011781 Print version: Gene expression systems. San Diego : Academic Press, ©1999 0122538404 9780122538407 (DLC) 98085621 (OCoLC)40462599 FWS01 ZDB-4-EBA FWS_PDA_EBA https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=207150 Volltext FWS01 ZDB-4-EBA FWS_PDA_EBA https://www.sciencedirect.com/science/book/9780122538407 Volltext |
spellingShingle | Gene expression systems : using nature for the art of expression / Contributors. -- Introduction: The Art of Expression. -- Purpose of This Book. -- Selecting a Suitable Expression System: -- Considerations. -- Genomics and the Future of Protein Expression Systems, J.M. Fernandez and J.P. Hoeffler. -- Prokaryotic Expression Systems: -- Gene Expression Systems Based on Bacteriophage T7 RNA Polymearse, R. Durbin. -- Expression Vectors Employing the trc Promoter, J. Brosius. -- Bacillus Expression: A Gram Positive Model, E. Ferrari and B. Miller. -- araB Expression System in Escherichia coli, M. Better. -- Eukaryotic Expression Systems: -- Adenoviral Vectors for Protein Expression, D.J. von Seggern and G.R. Nemerow. -- Expression in the Methalotrophic Yeast Pichia pastoris, J.M. Cregg. -- Recombinant Protein Expression in Pichia methanolica, C.K. Raymond. -- Cytomegalovirus Promoter for Expression in Mammalian Cells, M.F. Stinski. -- Inducible Mammalian Expression Systems, M. Russell. -- Protein Expression in Mammalian Cells Using Sindbis Virus, R.P. Bennett. -- Expression in Insect Systems: -- Drosophila S2 System for Heterologous Gene Expression, R.B. Kirkpatrick and A. Shatzman. -- Baculovirus Expression Vector System, M. Galleno and A.J. Sick. -- Transgenic Expression: -- Recombinant Protein Expression in Transgenic Mice, R. Abbud and J.H. Nilson. -- Expression of Recombinant Proteins in the Milk of Transgenic Animals, H.M. Meade, Y. Echelard, C.A. Ziomek, M.W. Young, M. Harvey, E.S. Cole, S. Groet, T.E. Smith, and J.M. Curling. -- Recombinant Protein Expression in Plants, A.E. Voloudakis, Y. Yin, and R.N. Beachy. -- Index. Gene expression systems based on bacteriophage T7 RNA polymerase -- Expression vectors employing the trc promoter -- Bacillus expression : a gram-positive model -- araB expression system in Escherichia coli -- Adenoviral vectors for protein expression -- Expression in the methylotrophic yeast Pichia pastoris -- Recombinant protein expression in Pichia methanolica -- Cytomegalovirus promoter for expression in mammalian cells -- Inducible mammalian expression systems -- Protein expression in mammalian cells using Sindbis virus -- Drosophila S2 system for heterologous gene expression -- Baculovirus expression vector system -- Recombinant protein expression in transgenic mice -- Expression of recombinant proteins in the milk of transgenic animals -- Recombinant protein expression in plants. Gene expression. http://id.loc.gov/authorities/subjects/sh85053734 Prokaryotes Genetics. Insects Genetics. Transgenic animals Genetics. Eukaryotic cells. http://id.loc.gov/authorities/subjects/sh85045546 Genetic regulation. http://id.loc.gov/authorities/subjects/sh85053870 Prokaryotic Cells Gene Expression Regulation Gene Expression Animals, Genetically Modified genetics Eukaryotic Cells Expression génique. Procaryotes Génétique. Insectes Génétique. Animaux transgéniques Génétique. Cellules eucaryotes. Régulation génétique. SCIENCE Life Sciences Genetics & Genomics. bisacsh Eukaryotic cells fast Gene expression fast Insects Genetics fast Prokaryotes Genetics fast Genexpressie. gtt Genregulatie. gtt Expression génique. ram Procaryotes Génétique. ram Insectes. ram Animaux transgéniques. ram Cellules eucaryotes. ram |
subject_GND | http://id.loc.gov/authorities/subjects/sh85053734 http://id.loc.gov/authorities/subjects/sh85045546 http://id.loc.gov/authorities/subjects/sh85053870 |
title | Gene expression systems : using nature for the art of expression / |
title_auth | Gene expression systems : using nature for the art of expression / |
title_exact_search | Gene expression systems : using nature for the art of expression / |
title_full | Gene expression systems : using nature for the art of expression / edited by Joseph M. Fernandez and James P. Hoeffler. |
title_fullStr | Gene expression systems : using nature for the art of expression / edited by Joseph M. Fernandez and James P. Hoeffler. |
title_full_unstemmed | Gene expression systems : using nature for the art of expression / edited by Joseph M. Fernandez and James P. Hoeffler. |
title_short | Gene expression systems : |
title_sort | gene expression systems using nature for the art of expression |
title_sub | using nature for the art of expression / |
topic | Gene expression. http://id.loc.gov/authorities/subjects/sh85053734 Prokaryotes Genetics. Insects Genetics. Transgenic animals Genetics. Eukaryotic cells. http://id.loc.gov/authorities/subjects/sh85045546 Genetic regulation. http://id.loc.gov/authorities/subjects/sh85053870 Prokaryotic Cells Gene Expression Regulation Gene Expression Animals, Genetically Modified genetics Eukaryotic Cells Expression génique. Procaryotes Génétique. Insectes Génétique. Animaux transgéniques Génétique. Cellules eucaryotes. Régulation génétique. SCIENCE Life Sciences Genetics & Genomics. bisacsh Eukaryotic cells fast Gene expression fast Insects Genetics fast Prokaryotes Genetics fast Genexpressie. gtt Genregulatie. gtt Expression génique. ram Procaryotes Génétique. ram Insectes. ram Animaux transgéniques. ram Cellules eucaryotes. ram |
topic_facet | Gene expression. Prokaryotes Genetics. Insects Genetics. Transgenic animals Genetics. Eukaryotic cells. Genetic regulation. Prokaryotic Cells Gene Expression Regulation Gene Expression Animals, Genetically Modified genetics Eukaryotic Cells Expression génique. Procaryotes Génétique. Insectes Génétique. Animaux transgéniques Génétique. Cellules eucaryotes. Régulation génétique. SCIENCE Life Sciences Genetics & Genomics. Eukaryotic cells Gene expression Insects Genetics Prokaryotes Genetics Genexpressie. Genregulatie. Insectes. Animaux transgéniques. |
url | https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=207150 https://www.sciencedirect.com/science/book/9780122538407 |
work_keys_str_mv | AT fernandezjosephm geneexpressionsystemsusingnaturefortheartofexpression AT hoefflerjamesp geneexpressionsystemsusingnaturefortheartofexpression |