Manipulation and expression of recombinant DNA :: a laboratory manual /
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hand...
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Burlington, MA :
Elsevier Academic Press,
©2006.
|
| Ausgabe: | 2nd ed. |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Zusammenfassung: | This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, |
| Beschreibung: | 1 online resource (xvi, 151 pages) : illustrations (some color) |
| Bibliographie: | Includes bibliographical references and index. |
| ISBN: | 0080456545 9780080456546 |
Internformat
MARC
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| 245 | 1 | 0 | |a Manipulation and expression of recombinant DNA : |b a laboratory manual / |c Susan Carson, Dominique Robertson. |
| 250 | |a 2nd ed. | ||
| 260 | |a Burlington, MA : |b Elsevier Academic Press, |c ©2006. | ||
| 300 | |a 1 online resource (xvi, 151 pages) : |b illustrations (some color) | ||
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| 504 | |a Includes bibliographical references and index. | ||
| 588 | 0 | |a Print version record. | |
| 505 | 0 | |a Cover; Contents; Preface; Acknowledgments; Note to Instructors; Instrumentation; Nomenclature; Introduction; Conceptual Outline for Experiments; Experimental Procedures; Laboratory Safety; General Operating Procedures; Emergency Contact Information; Part I; Manipulation of DNA; Lab Session 1; Getting Oriented; Practicing with Micropipettes; Station Checklist; Micropipetting; Laboratory Exercise; Preparing BSA Dilutions; Performing a Nitrocellulose Spot Test; Lab Session 2; Large-Scale Purification of Plasmid DNA; Introduction; Laboratory Exercises | |
| 505 | 8 | |a Alkaline Lysis and Anion Exchange ChromatograhyDNA Quantification; Lab Session 3; Preparation of Expression Vector DNA (pET-41a(+), a GST Fusion Protein Vector); Introduction; Laboratory Exercises; Restriction Digestion of Vector (pET41a) Restriction Enzyme Digestions; Agarose Gel Electrophoresis; Cleaning DNA Using a Spin Column; Lab Session 4; Preparation of Insert DNA (egfp); Introduction; Laboratory Exercises; Restriction Digestion of pEGFP-N1; Isolation of egfp DNA from Agarose; Lab Session 5; Preparation of Transformation-Competent Cells and Control Transformation; Introduction | |
| 505 | 8 | |a Laboratory Exercises Preparation of Chemically Competent Cells by Calcium Chloride Treatment; Transformation Control; Lab Session 6; DNA Ligation and Transformation of Escherichia coli; Introduction; Laboratory Exercises; Ligations and Ligation Controls; Divalent Cation-Mediated Transformation; Electrophoresis of Ligation Reactions; Part II; Screening Transformants; Lab Session 7; Colony Hybridizations; Lab Session 7A; Interim Laboratory Session; Introduction; Laboratory Exercises; Counting Transformants; Replica Plating; Lab Session 7B; Colony Hybridization: DNA Probe; Introduction | |
| 505 | 8 | |a Laboratory Exercises Colony Hybridization with an egfp DNA Probe: Part 1; Labeling of DNA Probe by PCR Using Digoxigenin-11-dUTP; Lab Session 7C; Colony Hybridization: Monoclonal Antibody Probe; Introduction; Laboratory Exercise; Colony Hybridization with an a-GFP Monoclonal Antibody Probe: Part 1; Lab Session 8; Completion of Colony Hybridization with DNA Probe; Introduction; Laboratory Exercise; Colony Hybridization with an egfp DNA Probe: Part 2; Lab Session 9; Characterization of Recombinant Clones; Lab Session 9A; Completion of Colony Hybridization with mAB Probe; Introduction | |
| 505 | 8 | |a Laboratory Exercise Colony Hybridization with an a-GFP monoclonal Antibody Probe: Part 2; Lab Session 9B; PCR Screening; Introduction; Polymerase Chain Reaction; Laboratory Exercise; Polymerase Chain Reaction Screen for Recombinant Clones: Part 1; Lab Session 9C; Visualization of Green Fluorescent Protein: Part 1; Introduction; Laboratory Exercise; Green Fluorescence Assay and Preparation of a Fresh Master Plate; Lab Session 10; Further Characterization of Recombinant Clones; Lab Session 10A:; Interim Laboratory Session; Laboratory Exercise; Inoculate Cultures for Minipreps; Lab Session 10B | |
| 520 | |a This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, | ||
| 650 | 0 | |a Recombinant DNA |v Laboratory manuals. | |
| 650 | 0 | |a Genetics |x Technique. |0 http://id.loc.gov/authorities/subjects/sh85053881 | |
| 650 | 1 | 2 | |a DNA, Recombinant |x analysis |0 https://id.nlm.nih.gov/mesh/D004274Q000032 |
| 650 | 1 | 2 | |a DNA, Recombinant |x isolation & purification |0 https://id.nlm.nih.gov/mesh/D004274Q000302 |
| 650 | 2 | 2 | |a Recombinant Proteins |x analysis |0 https://id.nlm.nih.gov/mesh/D011994Q000032 |
| 650 | 2 | 2 | |a Recombinant Proteins |x isolation & purification |0 https://id.nlm.nih.gov/mesh/D011994Q000302 |
| 650 | 2 | 2 | |a Genetic Techniques |0 https://id.nlm.nih.gov/mesh/D005821 |
| 650 | 6 | |a ADN recombinant |v Manuels de laboratoire. | |
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| 776 | 0 | 8 | |i Print version: |a Carson, Sue, Dr. |t Manipulation and expression of recombinant DNA. |b 2nd ed. |d Burlington, MA : Elsevier Academic Press, ©2006 |z 0120884186 |w (OCoLC)63293147 |
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| author | Carson, Sue (Susan) |
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| contents | Cover; Contents; Preface; Acknowledgments; Note to Instructors; Instrumentation; Nomenclature; Introduction; Conceptual Outline for Experiments; Experimental Procedures; Laboratory Safety; General Operating Procedures; Emergency Contact Information; Part I; Manipulation of DNA; Lab Session 1; Getting Oriented; Practicing with Micropipettes; Station Checklist; Micropipetting; Laboratory Exercise; Preparing BSA Dilutions; Performing a Nitrocellulose Spot Test; Lab Session 2; Large-Scale Purification of Plasmid DNA; Introduction; Laboratory Exercises Alkaline Lysis and Anion Exchange ChromatograhyDNA Quantification; Lab Session 3; Preparation of Expression Vector DNA (pET-41a(+), a GST Fusion Protein Vector); Introduction; Laboratory Exercises; Restriction Digestion of Vector (pET41a) Restriction Enzyme Digestions; Agarose Gel Electrophoresis; Cleaning DNA Using a Spin Column; Lab Session 4; Preparation of Insert DNA (egfp); Introduction; Laboratory Exercises; Restriction Digestion of pEGFP-N1; Isolation of egfp DNA from Agarose; Lab Session 5; Preparation of Transformation-Competent Cells and Control Transformation; Introduction Laboratory Exercises Preparation of Chemically Competent Cells by Calcium Chloride Treatment; Transformation Control; Lab Session 6; DNA Ligation and Transformation of Escherichia coli; Introduction; Laboratory Exercises; Ligations and Ligation Controls; Divalent Cation-Mediated Transformation; Electrophoresis of Ligation Reactions; Part II; Screening Transformants; Lab Session 7; Colony Hybridizations; Lab Session 7A; Interim Laboratory Session; Introduction; Laboratory Exercises; Counting Transformants; Replica Plating; Lab Session 7B; Colony Hybridization: DNA Probe; Introduction Laboratory Exercises Colony Hybridization with an egfp DNA Probe: Part 1; Labeling of DNA Probe by PCR Using Digoxigenin-11-dUTP; Lab Session 7C; Colony Hybridization: Monoclonal Antibody Probe; Introduction; Laboratory Exercise; Colony Hybridization with an a-GFP Monoclonal Antibody Probe: Part 1; Lab Session 8; Completion of Colony Hybridization with DNA Probe; Introduction; Laboratory Exercise; Colony Hybridization with an egfp DNA Probe: Part 2; Lab Session 9; Characterization of Recombinant Clones; Lab Session 9A; Completion of Colony Hybridization with mAB Probe; Introduction Laboratory Exercise Colony Hybridization with an a-GFP monoclonal Antibody Probe: Part 2; Lab Session 9B; PCR Screening; Introduction; Polymerase Chain Reaction; Laboratory Exercise; Polymerase Chain Reaction Screen for Recombinant Clones: Part 1; Lab Session 9C; Visualization of Green Fluorescent Protein: Part 1; Introduction; Laboratory Exercise; Green Fluorescence Assay and Preparation of a Fresh Master Plate; Lab Session 10; Further Characterization of Recombinant Clones; Lab Session 10A:; Interim Laboratory Session; Laboratory Exercise; Inoculate Cultures for Minipreps; Lab Session 10B |
| ctrlnum | (OCoLC)76831854 |
| dewey-full | 660.6/5 |
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| dewey-ones | 660 - Chemical engineering |
| dewey-raw | 660.6/5 |
| dewey-search | 660.6/5 |
| dewey-sort | 3660.6 15 |
| dewey-tens | 660 - Chemical engineering |
| discipline | Chemie / Pharmazie |
| edition | 2nd ed. |
| format | Electronic eBook |
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| genre_facet | Laboratory Manual Laboratory manuals Laboratory manuals. Manuels de laboratoire. |
| id | ZDB-4-EBA-ocm76831854 |
| illustrated | Illustrated |
| indexdate | 2024-11-27T13:15:58Z |
| institution | BVB |
| isbn | 0080456545 9780080456546 |
| language | English |
| oclc_num | 76831854 |
| open_access_boolean | |
| owner | MAIN DE-863 DE-BY-FWS |
| owner_facet | MAIN DE-863 DE-BY-FWS |
| physical | 1 online resource (xvi, 151 pages) : illustrations (some color) |
| psigel | ZDB-4-EBA |
| publishDate | 2006 |
| publishDateSearch | 2006 |
| publishDateSort | 2006 |
| publisher | Elsevier Academic Press, |
| record_format | marc |
| spelling | Carson, Sue (Susan) https://id.oclc.org/worldcat/entity/E39PCjJyhrG4vWDcgFJWv99DpX http://id.loc.gov/authorities/names/no2006087798 Manipulation and expression of recombinant DNA : a laboratory manual / Susan Carson, Dominique Robertson. 2nd ed. Burlington, MA : Elsevier Academic Press, ©2006. 1 online resource (xvi, 151 pages) : illustrations (some color) text txt rdacontent computer c rdamedia online resource cr rdacarrier Includes bibliographical references and index. Print version record. Cover; Contents; Preface; Acknowledgments; Note to Instructors; Instrumentation; Nomenclature; Introduction; Conceptual Outline for Experiments; Experimental Procedures; Laboratory Safety; General Operating Procedures; Emergency Contact Information; Part I; Manipulation of DNA; Lab Session 1; Getting Oriented; Practicing with Micropipettes; Station Checklist; Micropipetting; Laboratory Exercise; Preparing BSA Dilutions; Performing a Nitrocellulose Spot Test; Lab Session 2; Large-Scale Purification of Plasmid DNA; Introduction; Laboratory Exercises Alkaline Lysis and Anion Exchange ChromatograhyDNA Quantification; Lab Session 3; Preparation of Expression Vector DNA (pET-41a(+), a GST Fusion Protein Vector); Introduction; Laboratory Exercises; Restriction Digestion of Vector (pET41a) Restriction Enzyme Digestions; Agarose Gel Electrophoresis; Cleaning DNA Using a Spin Column; Lab Session 4; Preparation of Insert DNA (egfp); Introduction; Laboratory Exercises; Restriction Digestion of pEGFP-N1; Isolation of egfp DNA from Agarose; Lab Session 5; Preparation of Transformation-Competent Cells and Control Transformation; Introduction Laboratory Exercises Preparation of Chemically Competent Cells by Calcium Chloride Treatment; Transformation Control; Lab Session 6; DNA Ligation and Transformation of Escherichia coli; Introduction; Laboratory Exercises; Ligations and Ligation Controls; Divalent Cation-Mediated Transformation; Electrophoresis of Ligation Reactions; Part II; Screening Transformants; Lab Session 7; Colony Hybridizations; Lab Session 7A; Interim Laboratory Session; Introduction; Laboratory Exercises; Counting Transformants; Replica Plating; Lab Session 7B; Colony Hybridization: DNA Probe; Introduction Laboratory Exercises Colony Hybridization with an egfp DNA Probe: Part 1; Labeling of DNA Probe by PCR Using Digoxigenin-11-dUTP; Lab Session 7C; Colony Hybridization: Monoclonal Antibody Probe; Introduction; Laboratory Exercise; Colony Hybridization with an a-GFP Monoclonal Antibody Probe: Part 1; Lab Session 8; Completion of Colony Hybridization with DNA Probe; Introduction; Laboratory Exercise; Colony Hybridization with an egfp DNA Probe: Part 2; Lab Session 9; Characterization of Recombinant Clones; Lab Session 9A; Completion of Colony Hybridization with mAB Probe; Introduction Laboratory Exercise Colony Hybridization with an a-GFP monoclonal Antibody Probe: Part 2; Lab Session 9B; PCR Screening; Introduction; Polymerase Chain Reaction; Laboratory Exercise; Polymerase Chain Reaction Screen for Recombinant Clones: Part 1; Lab Session 9C; Visualization of Green Fluorescent Protein: Part 1; Introduction; Laboratory Exercise; Green Fluorescence Assay and Preparation of a Fresh Master Plate; Lab Session 10; Further Characterization of Recombinant Clones; Lab Session 10A:; Interim Laboratory Session; Laboratory Exercise; Inoculate Cultures for Minipreps; Lab Session 10B This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, Recombinant DNA Laboratory manuals. Genetics Technique. http://id.loc.gov/authorities/subjects/sh85053881 DNA, Recombinant analysis https://id.nlm.nih.gov/mesh/D004274Q000032 DNA, Recombinant isolation & purification https://id.nlm.nih.gov/mesh/D004274Q000302 Recombinant Proteins analysis https://id.nlm.nih.gov/mesh/D011994Q000032 Recombinant Proteins isolation & purification https://id.nlm.nih.gov/mesh/D011994Q000302 Genetic Techniques https://id.nlm.nih.gov/mesh/D005821 ADN recombinant Manuels de laboratoire. Génétique Technique. SCIENCE Biotechnology. bisacsh Genetics Technique fast Recombinant DNA fast Laboratory Manual https://id.nlm.nih.gov/mesh/D020484 Laboratory manuals fast Laboratory manuals. lcgft http://id.loc.gov/authorities/genreForms/gf2014026120 Manuels de laboratoire. rvmgf Robertson, Dominique, 1951- https://id.oclc.org/worldcat/entity/E39PCjGqwBt7GkXDVTWFjw47Dy http://id.loc.gov/authorities/names/n90605676 Print version: Carson, Sue, Dr. Manipulation and expression of recombinant DNA. 2nd ed. Burlington, MA : Elsevier Academic Press, ©2006 0120884186 (OCoLC)63293147 FWS01 ZDB-4-EBA FWS_PDA_EBA https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=166366 Volltext |
| spellingShingle | Carson, Sue (Susan) Manipulation and expression of recombinant DNA : a laboratory manual / Cover; Contents; Preface; Acknowledgments; Note to Instructors; Instrumentation; Nomenclature; Introduction; Conceptual Outline for Experiments; Experimental Procedures; Laboratory Safety; General Operating Procedures; Emergency Contact Information; Part I; Manipulation of DNA; Lab Session 1; Getting Oriented; Practicing with Micropipettes; Station Checklist; Micropipetting; Laboratory Exercise; Preparing BSA Dilutions; Performing a Nitrocellulose Spot Test; Lab Session 2; Large-Scale Purification of Plasmid DNA; Introduction; Laboratory Exercises Alkaline Lysis and Anion Exchange ChromatograhyDNA Quantification; Lab Session 3; Preparation of Expression Vector DNA (pET-41a(+), a GST Fusion Protein Vector); Introduction; Laboratory Exercises; Restriction Digestion of Vector (pET41a) Restriction Enzyme Digestions; Agarose Gel Electrophoresis; Cleaning DNA Using a Spin Column; Lab Session 4; Preparation of Insert DNA (egfp); Introduction; Laboratory Exercises; Restriction Digestion of pEGFP-N1; Isolation of egfp DNA from Agarose; Lab Session 5; Preparation of Transformation-Competent Cells and Control Transformation; Introduction Laboratory Exercises Preparation of Chemically Competent Cells by Calcium Chloride Treatment; Transformation Control; Lab Session 6; DNA Ligation and Transformation of Escherichia coli; Introduction; Laboratory Exercises; Ligations and Ligation Controls; Divalent Cation-Mediated Transformation; Electrophoresis of Ligation Reactions; Part II; Screening Transformants; Lab Session 7; Colony Hybridizations; Lab Session 7A; Interim Laboratory Session; Introduction; Laboratory Exercises; Counting Transformants; Replica Plating; Lab Session 7B; Colony Hybridization: DNA Probe; Introduction Laboratory Exercises Colony Hybridization with an egfp DNA Probe: Part 1; Labeling of DNA Probe by PCR Using Digoxigenin-11-dUTP; Lab Session 7C; Colony Hybridization: Monoclonal Antibody Probe; Introduction; Laboratory Exercise; Colony Hybridization with an a-GFP Monoclonal Antibody Probe: Part 1; Lab Session 8; Completion of Colony Hybridization with DNA Probe; Introduction; Laboratory Exercise; Colony Hybridization with an egfp DNA Probe: Part 2; Lab Session 9; Characterization of Recombinant Clones; Lab Session 9A; Completion of Colony Hybridization with mAB Probe; Introduction Laboratory Exercise Colony Hybridization with an a-GFP monoclonal Antibody Probe: Part 2; Lab Session 9B; PCR Screening; Introduction; Polymerase Chain Reaction; Laboratory Exercise; Polymerase Chain Reaction Screen for Recombinant Clones: Part 1; Lab Session 9C; Visualization of Green Fluorescent Protein: Part 1; Introduction; Laboratory Exercise; Green Fluorescence Assay and Preparation of a Fresh Master Plate; Lab Session 10; Further Characterization of Recombinant Clones; Lab Session 10A:; Interim Laboratory Session; Laboratory Exercise; Inoculate Cultures for Minipreps; Lab Session 10B Recombinant DNA Laboratory manuals. Genetics Technique. http://id.loc.gov/authorities/subjects/sh85053881 DNA, Recombinant analysis https://id.nlm.nih.gov/mesh/D004274Q000032 DNA, Recombinant isolation & purification https://id.nlm.nih.gov/mesh/D004274Q000302 Recombinant Proteins analysis https://id.nlm.nih.gov/mesh/D011994Q000032 Recombinant Proteins isolation & purification https://id.nlm.nih.gov/mesh/D011994Q000302 Genetic Techniques https://id.nlm.nih.gov/mesh/D005821 ADN recombinant Manuels de laboratoire. Génétique Technique. SCIENCE Biotechnology. bisacsh Genetics Technique fast Recombinant DNA fast |
| subject_GND | http://id.loc.gov/authorities/subjects/sh85053881 https://id.nlm.nih.gov/mesh/D004274Q000032 https://id.nlm.nih.gov/mesh/D004274Q000302 https://id.nlm.nih.gov/mesh/D011994Q000032 https://id.nlm.nih.gov/mesh/D011994Q000302 https://id.nlm.nih.gov/mesh/D005821 https://id.nlm.nih.gov/mesh/D020484 http://id.loc.gov/authorities/genreForms/gf2014026120 |
| title | Manipulation and expression of recombinant DNA : a laboratory manual / |
| title_auth | Manipulation and expression of recombinant DNA : a laboratory manual / |
| title_exact_search | Manipulation and expression of recombinant DNA : a laboratory manual / |
| title_full | Manipulation and expression of recombinant DNA : a laboratory manual / Susan Carson, Dominique Robertson. |
| title_fullStr | Manipulation and expression of recombinant DNA : a laboratory manual / Susan Carson, Dominique Robertson. |
| title_full_unstemmed | Manipulation and expression of recombinant DNA : a laboratory manual / Susan Carson, Dominique Robertson. |
| title_short | Manipulation and expression of recombinant DNA : |
| title_sort | manipulation and expression of recombinant dna a laboratory manual |
| title_sub | a laboratory manual / |
| topic | Recombinant DNA Laboratory manuals. Genetics Technique. http://id.loc.gov/authorities/subjects/sh85053881 DNA, Recombinant analysis https://id.nlm.nih.gov/mesh/D004274Q000032 DNA, Recombinant isolation & purification https://id.nlm.nih.gov/mesh/D004274Q000302 Recombinant Proteins analysis https://id.nlm.nih.gov/mesh/D011994Q000032 Recombinant Proteins isolation & purification https://id.nlm.nih.gov/mesh/D011994Q000302 Genetic Techniques https://id.nlm.nih.gov/mesh/D005821 ADN recombinant Manuels de laboratoire. Génétique Technique. SCIENCE Biotechnology. bisacsh Genetics Technique fast Recombinant DNA fast |
| topic_facet | Recombinant DNA Laboratory manuals. Genetics Technique. DNA, Recombinant analysis DNA, Recombinant isolation & purification Recombinant Proteins analysis Recombinant Proteins isolation & purification Genetic Techniques ADN recombinant Manuels de laboratoire. Génétique Technique. SCIENCE Biotechnology. Genetics Technique Recombinant DNA Laboratory Manual Laboratory manuals Laboratory manuals. Manuels de laboratoire. |
| url | https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=166366 |
| work_keys_str_mv | AT carsonsue manipulationandexpressionofrecombinantdnaalaboratorymanual AT robertsondominique manipulationandexpressionofrecombinantdnaalaboratorymanual |