Double-Stranded RNA: Methods and Protocols
This detailed volume examines classical and cutting-edge methods involving double-stranded RNA (dsRNA), specifically regarding isolation, visualization, characterization, production, and application. Many protocols, such as co-immunoprecipitation-based isolation of double-stranded RNA-associated pro...
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| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
New York, NY
Humana
2025
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| Series: | Methods in Molecular Biology
2771 |
| Subjects: | |
| Summary: | This detailed volume examines classical and cutting-edge methods involving double-stranded RNA (dsRNA), specifically regarding isolation, visualization, characterization, production, and application. Many protocols, such as co-immunoprecipitation-based isolation of double-stranded RNA-associated protein complexes, identification of mycoviruses by dsRNA extraction, application of dsRNA for fungi disease management (Sclerotinia sclerotiorum and Botrytis cin), and production of double-stranded RNA in plants by plant viral vectors for gene silencing, can also be easily adapted for identification of viruses from other organisms, control of other pathogens, and fundamental research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips for troubleshooting and avoiding known pitfalls. Reliable and practical, Double-Stranded RNA: Methods and Protocols serves as an ideal reference book for students and researchers who work with dsRNA. |
| Item Description: | XI, 142 p. 31 illus., 20 illus. in color |
| Physical Description: | 142 Seiten 254 mm |
| ISBN: | 9781071637043 |
Staff View
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| spelling | Cheng, Xiaofei Verfasser aut Double-Stranded RNA Methods and Protocols New York, NY Humana 2025 142 Seiten 254 mm txt rdacontent n rdamedia nc rdacarrier Methods in Molecular Biology 2771 XI, 142 p. 31 illus., 20 illus. in color This detailed volume examines classical and cutting-edge methods involving double-stranded RNA (dsRNA), specifically regarding isolation, visualization, characterization, production, and application. Many protocols, such as co-immunoprecipitation-based isolation of double-stranded RNA-associated protein complexes, identification of mycoviruses by dsRNA extraction, application of dsRNA for fungi disease management (Sclerotinia sclerotiorum and Botrytis cin), and production of double-stranded RNA in plants by plant viral vectors for gene silencing, can also be easily adapted for identification of viruses from other organisms, control of other pathogens, and fundamental research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips for troubleshooting and avoiding known pitfalls. Reliable and practical, Double-Stranded RNA: Methods and Protocols serves as an ideal reference book for students and researchers who work with dsRNA. bicssc bisacsh Genetics Virology RNA—Metabolism Hardcover, Softcover / Biologie/Genetik, Gentechnik Wu, Guanwei Sonstige oth |
| spellingShingle | Cheng, Xiaofei Double-Stranded RNA Methods and Protocols bicssc bisacsh Genetics Virology RNA—Metabolism |
| title | Double-Stranded RNA Methods and Protocols |
| title_auth | Double-Stranded RNA Methods and Protocols |
| title_exact_search | Double-Stranded RNA Methods and Protocols |
| title_full | Double-Stranded RNA Methods and Protocols |
| title_fullStr | Double-Stranded RNA Methods and Protocols |
| title_full_unstemmed | Double-Stranded RNA Methods and Protocols |
| title_short | Double-Stranded RNA |
| title_sort | double stranded rna methods and protocols |
| title_sub | Methods and Protocols |
| topic | bicssc bisacsh Genetics Virology RNA—Metabolism |
| topic_facet | bicssc bisacsh Genetics Virology RNA—Metabolism |
| work_keys_str_mv | AT chengxiaofei doublestrandedrnamethodsandprotocols AT wuguanwei doublestrandedrnamethodsandprotocols |