Double-stranded RNA: methods and protocols
This detailed volume examines classical and cutting-edge methods involving double-stranded RNA (dsRNA), specifically regarding isolation, visualization, characterization, production, and application. Many protocols, such as co-immunoprecipitation-based isolation of double-stranded RNA-associated pro...
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Weitere Verfasser: | |
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
HumanaPress
[2024]
|
| Schriftenreihe: | Methods in molecular biology
2771 |
| Schlagworte: | |
| Zusammenfassung: | This detailed volume examines classical and cutting-edge methods involving double-stranded RNA (dsRNA), specifically regarding isolation, visualization, characterization, production, and application. Many protocols, such as co-immunoprecipitation-based isolation of double-stranded RNA-associated protein complexes, identification of mycoviruses by dsRNA extraction, application of dsRNA for fungi disease management (Sclerotinia sclerotiorum and Botrytis cin), and production of double-stranded RNA in plants by plant viral vectors for gene silencing, can also be easily adapted for identification of viruses from other organisms, control of other pathogens, and fundamental research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips for troubleshooting and avoiding known pitfalls. Reliable and practical, Double-Stranded RNA: Methods and Protocols serves as an ideal reference book for students and researchers who work with dsRNA. |
| Beschreibung: | xi, 142 Seiten 254 mm |
| ISBN: | 9781071637043 |
Internformat
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| 520 | |a This detailed volume examines classical and cutting-edge methods involving double-stranded RNA (dsRNA), specifically regarding isolation, visualization, characterization, production, and application. Many protocols, such as co-immunoprecipitation-based isolation of double-stranded RNA-associated protein complexes, identification of mycoviruses by dsRNA extraction, application of dsRNA for fungi disease management (Sclerotinia sclerotiorum and Botrytis cin), and production of double-stranded RNA in plants by plant viral vectors for gene silencing, can also be easily adapted for identification of viruses from other organisms, control of other pathogens, and fundamental research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips for troubleshooting and avoiding known pitfalls. Reliable and practical, Double-Stranded RNA: Methods and Protocols serves as an ideal reference book for students and researchers who work with dsRNA. | ||
| 650 | 4 | |a Genetics | |
| 650 | 4 | |a Virology | |
| 650 | 4 | |a RNA—Metabolism | |
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Datensatz im Suchindex
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| publishDate | 2024 |
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| spelling | Double-stranded RNA methods and protocols edited by Xiaofei Cheng, Guanwei Wu New York, NY HumanaPress [2024] xi, 142 Seiten 254 mm txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 2771 Springer protocols This detailed volume examines classical and cutting-edge methods involving double-stranded RNA (dsRNA), specifically regarding isolation, visualization, characterization, production, and application. Many protocols, such as co-immunoprecipitation-based isolation of double-stranded RNA-associated protein complexes, identification of mycoviruses by dsRNA extraction, application of dsRNA for fungi disease management (Sclerotinia sclerotiorum and Botrytis cin), and production of double-stranded RNA in plants by plant viral vectors for gene silencing, can also be easily adapted for identification of viruses from other organisms, control of other pathogens, and fundamental research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips for troubleshooting and avoiding known pitfalls. Reliable and practical, Double-Stranded RNA: Methods and Protocols serves as an ideal reference book for students and researchers who work with dsRNA. Genetics Virology RNA—Metabolism Hardcover, Softcover / Biologie/Genetik, Gentechnik (DE-588)4143413-4 Aufsatzsammlung gnd-content Cheng, Xiaofei edt Wu, Guanwei edt Erscheint auch als Online-Ausgabe 978-1-0716-3702-9 Methods in molecular biology 2771 (DE-604)BV035362695 2771 |
| spellingShingle | Cheng, Xiaofei Double-stranded RNA methods and protocols Genetics Virology RNA—Metabolism Methods in molecular biology |
| subject_GND | (DE-588)4143413-4 |
| title | Double-stranded RNA methods and protocols |
| title_auth | Double-stranded RNA methods and protocols |
| title_exact_search | Double-stranded RNA methods and protocols |
| title_full | Double-stranded RNA methods and protocols edited by Xiaofei Cheng, Guanwei Wu |
| title_fullStr | Double-stranded RNA methods and protocols edited by Xiaofei Cheng, Guanwei Wu |
| title_full_unstemmed | Double-stranded RNA methods and protocols edited by Xiaofei Cheng, Guanwei Wu |
| title_short | Double-stranded RNA |
| title_sort | double stranded rna methods and protocols |
| title_sub | methods and protocols |
| topic | Genetics Virology RNA—Metabolism |
| topic_facet | Genetics Virology RNA—Metabolism Aufsatzsammlung |
| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT chengxiaofei doublestrandedrnamethodsandprotocols AT wuguanwei doublestrandedrnamethodsandprotocols |