Host defense mechanisms against pathogens mediated by sphingolipids:
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| 245 | 1 | 0 | |a Host defense mechanisms against pathogens mediated by sphingolipids |c vorgelegt dem Rat der Medizinischen Fakultät der Friedrich-Schiller-Universität Jena von Tina Müller |
| 264 | 1 | |a Jena |c 14.03.2023 | |
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Datensatz im Suchindex
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| adam_txt |
TABLE
OF
CONTENT
ABBREVIATIONS
.
VII
ABSTRACT
.
X
ZUSAMMENFASSUNG
.
XII
1
INTRODUCTION
.
1
1.1
SPHINGOLIPIDS
.
1
1.1.1
SPHINGOLIPID
METABOLISM
IN
EUKARYOTES
.
1
1.1.1.1
SL
METABOLISM
SPECIFIC
TO
MAMMALIAN
HOST
.
2
1.1.1.2
SL
METABOLISM
SPECIFIC
TO
OPPORTUNISTIC
PATHOGENIC
YEAST
.
3
1.1.2
SPHINGOLIPID
FUNCTIONS
IN
EUKARYOTES
.
4
1.1.2.1
SPECIE-SPECIFIC
FUNCTIONS
OF
SLS
.
5
1.2
HUMAN
OPPORTUNISTIC
PATHOGENS
.
7
1.2.1
VIRULENCE
OF
HUMAN
OPPORTUNISTIC
PATHOGENS
.
8
1.2.2
ROLE
OF
HOST
SLS
IN
INFECTION
OF
OPPORTUNISTIC
PATHOGENS
.
12
1.3
AIM
OF
THE
DOCTORAL
THESIS
.
14
2
MATERIALS
AND
METHODS
.
15
2.1
MATERIALS
.
15
2.1.1
BACTERIAL
AND
FUNGAL
STRAINS,
CELL
LINES
.
15
2.1.1.1
STRAINS
.
15
2.1.1.2
CELL
LINES
.
15
2.1.2
CONSUMABLES,
DEVICES,
CHEMICALS
.
16
2.1.3
MEDIA,
BUFFERS,
SOLUTIONS
.
20
2.1.3.1
GROWTH
MEDIA
FOR
CULTIVATION
OF
MICROBES
.
21
2.1.3.2
BASAL
MEDIA
FOR
CULTIVATION
OF
HUMAN
CELL
LINES
.
21
2.1.3.3
WESTERN
BLOTTING
.
21
2.1.3.4
INTERLEUKIN
SECRETION
ANALYSIS
.
24
2.1.3.5
PROTEIN
KINASE
C
LIKE
1
ACTIVITY
ASSAY
.
24
2.1.3.6
SOLUTIONS
.
25
2.1.4
PEPTIDE
.
27
2.1.5
ANTIBODIES
FOR
WESTERN
BLOTTING
.
27
2.1.6
KITS
.
28
2.1.7
SOFTWARE
.
28
2.2
METHODS
.
28
2.2.1
SPHINGOLIPID
PROFILES
FROM
NON-INFECTED
AND
INFECTED
MICE
.
28
2.2.1.1
MICE
.
28
III
2.2.1.2
ORGAN
HARVEST
.
29
2.2.2
CELL
CULTURE
.
29
2.2.2.1
BACTERIA
.
29
2.2.2.2
E.
COLI
STIMULATION
WITH
SPHINGOSINE
AND
PHOSPHATIDYLSERINE
.
29
2.2.2.3
PREPARATION
FOR
ELECTRON
MICROSCOPY
.
30
2.2.2.4
FUNGI
.
30
2.2.2.5
GROWTH
ANALYSIS
OF
C.
ALBICANS
SC53
14
YEAST
CHALLENGED
WITH
SPHINGOLIPIDS
.
31
2.2.2.6
GROWTH
ANALYSIS
OF
C.
ALBICANS
SC5314
HYPHAE
CHALLENGED
WITH
SPHINGOLIPIDS
.
31
2.2.2.7
ECEI
EXPRESSION
ANALYSIS
IN
C.
ALBICANS
SC53
14
CHALLENGED WITH
SPHINGOSINE
.
32
2.2.2.8
METABOLISM
ANALYSIS
OF
EXOGENOUS
SPHINGOSINE
.
33
2.2.3
APPLICATION
OF
SPHINGOSINE
OR FTY720
TREATMENT
IN
AN
INTESTINAL
EPITHELIAL
CELL
INFECTION
MODEL
.
33
2.2.3.1
CULTURE
AND
DIFFERENTIATION
OF
C2BBEL
INTESTINAL
EPITHELIAL
CELLS
.
33
2.2.3.2
INFECTION
OF
POLARIZED
C2BBEL
INTESTINAL
EPITHELIAL
CELLS
WITH
PRE-TREATED
C.
ALBICANS
SC5314
.
34
2.2.3.3
TRANSEPITHELIAL
ELECTRICAL
RESISTANCE
MEASUREMENT
.
34
2.2.3.4
WESTERN
BLOTTING
ANALYSIS
OF
TIGHT
JUNCTION
AND
JUNCTIONAL
PROTEIN
EXPRESSION
.
35
2.2.3.4.1
PROTEIN
ISOLATION,
QUANTIFICATION
AND
PREPARATION
.
35
2.2.3.4.2
GEL
ELECTROPHORESIS
(SDS-PAGE)
.
35
2.2.3.4.3
WESTERN
BLOTTING
.
36
2.2.3.4.4
IMMUNODETECTION
.
36
2.2.3.4.5
MEMBRANE
RE-PROBING
.
36
2.2.3.4.6
SIGNAL
INTENSITY
QUANTIFICATION
.
37
2.2.3.6
QUANTIFICATION
OF
INTERLEUKIN
SECRETION
FROM
NON
AND
INFECTED
C2BBEL
IEC
S
.
37
2.2.3.7
SPHINGOLIPID
METABOLISM
ANALYSIS
OF
PRELOADED
SC5314
CELLS
.
39
2.2.4
LIPID
EXTRACTION
.
39
2.2.4.1
TISSUE
HOMOGENISATION
.
39
2.2.4.2
LIPID
EXTRACTION
FROM
MICE
ORGANS
.
40
2.2.4.3
LIPID
EXTRACTION
FROM
CELL
CULTURE
SAMPLES
.
40
2.2.5
LC-MS/MS
ANALYSIS
.
40
2.2.6
PROTEIN
KINASE
C-LIKE
1
ACTIVITY
IN
C.
ALBICANS
UNDER
SPHINGOLIPID
TREATMENT.
43
2.2.6.1
CULTIVATION
OF
C.
ALBICANS
SC5314,
SN95,
CAL
1255
YEAST
.
43
IV
2.2.6.2
YEAST
PROTEIN
EXTRACTION
AND
QUANTIFICATION
.
43
2.2.6.3
SIGNATECT
PROTEIN
KINASE
C
ASSAY
SYSTEM
.
44
2.2.7
STATISTICAL
ANALYSIS
.
45
3
RESULTS
.
47
3.1
HOST
SPHINGOLIPID
PROFILES
IN
PHYSIOLOGICAL
AND
INFECTIOUS
STATE
.
47
3.1.1
PHYSIOLOGICAL
SPHINGOLIPID
PROFILE
OF
C5
7BL/6J
MICE
.
47
3.1.2
SPHINGOLIPID
PROFILES
OF
FEMALE
BALB/C
MICE
INFECTED
WITH
C.
ALBICANS
.
50
3.2
ANTIBACTERIAL
POTENTIAL
OF
SPHINGOSINE
.
52
3.2.1
INHIBITION
OF
E.
COLI
GROWTH
BY
SPHINGOSINE
.
52
3.2.2
RESCUE
FROM
SPH
INDUCED
GROWTH
INHIBITION
BY
PHOSPHATIDYLSERINE
.
54
3.3
EFFECTS
OF
SPHINGOLIPID
TREATMENT
ON
C.
ALBICANS
SC53
14
MORPHOGENESIS
.
55
3.3.1
EFFECTS
OF
SPHINGOLIPID
TREATMENT
ON
C.
ALBICANS
SC53
14
YEAST
GROWTH
.
55
3.3.2
EFFECTS
OF
SPHINGOLIPID
CHALLENGE
ON
C.
ALBICANS
SC53
14
HYPHAL
GROWTH
.
59
3.3.3
METABOLISM
OF
EXOGENOUS
SPHINGOSINE
IN
SC53
14
YEAST
AND
HYPHAE
.
69
3.4
ALTERATION
OF
PROTEIN
KINASE
ACTIVITY
IN
C.
ALBICANS
BY
SPHINGOSINE
.
73
3.4.1
INCREASE
OF
C.
ALBICANS
PROTEIN
KINASE
ACTIVITY
BY
SPHINGOSINE
.
74
3.5
EFFECT
OF
SPH
INDUCED
HYPHAL
GROWTH
POSTPONEMENT
ON
FUNGAL
INFECTION
DEVELOPMENT
IN
C2BBEL
IECS
.
76
3.5.1
PRELOADING
OF
C.
ALBICANS
SC53
14
YEAST
WITH
SPH
AND
FTY720
PRIOR
TO
INFECTION
.
77
3.5.2
SPH
PROLONGS
MAINTENANCE
OF
INTESTINAL
EPITHELIAL
BARRIER
STABILITY
DURING
C.
ALBICANS
INFECTION
.
78
3.5.2.1
SPH
POSTPONES
MEMBRANE
INTEGRITY
DECREASE
DURING
C.
ALBICANS
INFECTION
.
78
3.5.2.2
POSTPONED
MEMBRANE
BARRIER
BREAKDOWN
IS
NOT
CAUSED
BY
EXTRACELLULARLY
SIP
INCREASE
.
79
3.5.2.3
TIGHT
JUNCTION
AND
ADHERENCE
JUNCTION
EXPRESSION
.
82
3.5.3
INTERLEUKIN
SECRETION
.
84
4
DISCUSSION
.
86
4.1
PHYSIOLOGICAL
SPHINGOSINE
CONCENTRATIONS
AS
GUARD
AT
PATHOGEN
ENTRY
PORTALS
.
86
4.2
SPHINGOSINE
LEVELS
ARE
NOT
ALTERED
IN
FUNGAL
CHRONICAL
INFECTION
.
88
4.3
HIGHLY
CONCENTRATED
EXOGENOUS
SPHINGOLIPIDS
ALTER
BACTERIAL
GROWTH
.
89
4.4
SPH
IN
HOST
PHYSIOLOGICAL CONCENTRATION
ALTERS
FUNGAL
MORPHOGENESIS
.
90
4.5
APPLICATION
OF
HYPHAL
GROWTH
INDUCTION
INHIBITION
BY
SPH
IN
AN
INTESTINAL
INFECTION
MODEL
.
94
5
CONCLUSION
AND
OUTLOOK
.
98
LITERATURE
.
XIV
APPENDIX
.
XXXV
V
SUPPLEMENTARY
FIGURES
.
XXXV
LIST
OF
TABLES
.
XLVI
LIST
OF
FIGURES
.
XLVII
CURRICULUM
VITAE
.
XLIX
ACKNOWLEDGEMENT
.
XLIX
EHRENWOERTLICHE
ERKLAERUNG
.
LILI |
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| indexdate | 2024-07-10T10:12:23Z |
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| language | English |
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| physical | 98, LIII Seiten Illustrationen, Diagramme 31 cm |
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| spelling | Müller, Tina 1989- Verfasser (DE-588)1217345760 aut Host defense mechanisms against pathogens mediated by sphingolipids vorgelegt dem Rat der Medizinischen Fakultät der Friedrich-Schiller-Universität Jena von Tina Müller Jena 14.03.2023 98, LIII Seiten Illustrationen, Diagramme 31 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Friedrich-Schiller-Universität Jena 2023 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf https://d-nb.info/1301169072/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=034966372&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p dnb 20231206 DE-101 https://d-nb.info/provenance/plan#dnb |
| spellingShingle | Müller, Tina 1989- Host defense mechanisms against pathogens mediated by sphingolipids |
| subject_GND | (DE-588)4113937-9 |
| title | Host defense mechanisms against pathogens mediated by sphingolipids |
| title_auth | Host defense mechanisms against pathogens mediated by sphingolipids |
| title_exact_search | Host defense mechanisms against pathogens mediated by sphingolipids |
| title_exact_search_txtP | Host defense mechanisms against pathogens mediated by sphingolipids |
| title_full | Host defense mechanisms against pathogens mediated by sphingolipids vorgelegt dem Rat der Medizinischen Fakultät der Friedrich-Schiller-Universität Jena von Tina Müller |
| title_fullStr | Host defense mechanisms against pathogens mediated by sphingolipids vorgelegt dem Rat der Medizinischen Fakultät der Friedrich-Schiller-Universität Jena von Tina Müller |
| title_full_unstemmed | Host defense mechanisms against pathogens mediated by sphingolipids vorgelegt dem Rat der Medizinischen Fakultät der Friedrich-Schiller-Universität Jena von Tina Müller |
| title_short | Host defense mechanisms against pathogens mediated by sphingolipids |
| title_sort | host defense mechanisms against pathogens mediated by sphingolipids |
| topic_facet | Hochschulschrift |
| url | https://d-nb.info/1301169072/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=034966372&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT mullertina hostdefensemechanismsagainstpathogensmediatedbysphingolipids |
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