Proteomics mass spectrometry methods: sample preparation, protein digestion, and research protocols
Front Cover -- Proteomics Mass Spectrometry Methods -- Front Matter -- Copyright -- Contents -- Contributors -- I - Introduction -- 1 - Book introduction -- References -- 2 - Introduction to sample preparation for proteomics and mass spectrometry -- 1. Introduction -- 2. Sample preparation for prote...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
London ; San Diego ; Cambridge
Academic Press, an imprint of Elsevier
[2024]
|
| Schlagworte: | |
| Online-Zugang: | TUM01 |
| Zusammenfassung: | Front Cover -- Proteomics Mass Spectrometry Methods -- Front Matter -- Copyright -- Contents -- Contributors -- I - Introduction -- 1 - Book introduction -- References -- 2 - Introduction to sample preparation for proteomics and mass spectrometry -- 1. Introduction -- 2. Sample preparation for proteomic analysis -- 2.1 Sample fractionation -- 2.1.1 Immunodepletion for clinical proteomics -- 2.1.2 Subcellular fractionation and enrichment -- 2.2 Proteolytic digestion for proteomic analysis -- 3. Application of mass spectrometry for the analysis of proteins and peptides -- 3.1 Bottom up/shotgun proteomics -- 3.1.1 Data-dependent acquisition -- 3.1.2 Data-independent acquisition -- 3.2 MS/MS fragmentation strategies -- 3.2.1 Collision-induced dissociation -- 3.2.2 High collision dissociation -- 3.2.3 Neutral loss MS3 -- 3.2.4 Electron transfer dissociation -- 3.2.5 Multistage activation -- 3.3 Identifying peptides from mass spectra -- 3.4 Sequence databases -- 3.5 Mass spectrometry methods for differential proteomics -- 3.5.1 Label-free differential expression proteomics -- 3.5.2 Stable isotopes tags/isobaric mass tags -- 3.6 Mass spectrometry for quantitative targeted proteomics -- 4. Bioinformatic analysis of proteomic data -- 5. Conclusions -- References -- II - Sample preparation -- 3 - Sample preparation for proteomics and mass spectrometry from mammalian cell lines -- 1. Introduction -- 2. Materials and equipment -- 3. Before you begin -- 3.1 Filter-aided sample preparation -- 3.2 PreOmics iST kit -- 3.3 Membrane fractionation -- 3.4 High pH reversed-phase fractionation -- 3.5 Peptide purification and fractionation: strong cation exchange -- 3.6 Tandem Mass Tag (TMT) labeling -- 4. Step-by-step method details -- 4.1 Cell culture and collection of cell pellets for proteomics -- 4.2 Filter-aided sample preparation -- 4.3 PreOmics iST protocol. |
| Beschreibung: | Description based on publisher supplied metadata and other sources |
| Beschreibung: | 1 Online-Ressource Illustrationen, Diagramme |
| ISBN: | 9780323906524 |
Internformat
MARC
| LEADER | 00000nmm a22000001c 4500 | ||
|---|---|---|---|
| 001 | BV049600236 | ||
| 003 | DE-604 | ||
| 005 | 20240306 | ||
| 007 | cr|uuu---uuuuu | ||
| 008 | 240306s2024 |||| o||u| ||||||eng d | ||
| 020 | |a 9780323906524 |9 978-0-323-90652-4 | ||
| 035 | |a (OCoLC)1427317648 | ||
| 035 | |a (DE-599)KEP100841015 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-91G | ||
| 082 | 0 | |a 572.6 | |
| 084 | |a CHE 254 |2 stub | ||
| 084 | |a CHE 820 |2 stub | ||
| 084 | |a CHE 808 |2 stub | ||
| 245 | 1 | 0 | |a Proteomics mass spectrometry methods |b sample preparation, protein digestion, and research protocols |c edited by Paula Meleady, National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin, Ireland, School of Biotechnology, Dublin City University, Glasnevin, Dublin, Ireland |
| 264 | 1 | |a London ; San Diego ; Cambridge |b Academic Press, an imprint of Elsevier |c [2024] | |
| 300 | |a 1 Online-Ressource |b Illustrationen, Diagramme | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b c |2 rdamedia | ||
| 338 | |b cr |2 rdacarrier | ||
| 500 | |a Description based on publisher supplied metadata and other sources | ||
| 520 | 3 | |a Front Cover -- Proteomics Mass Spectrometry Methods -- Front Matter -- Copyright -- Contents -- Contributors -- I - Introduction -- 1 - Book introduction -- References -- 2 - Introduction to sample preparation for proteomics and mass spectrometry -- 1. Introduction -- 2. Sample preparation for proteomic analysis -- 2.1 Sample fractionation -- 2.1.1 Immunodepletion for clinical proteomics -- 2.1.2 Subcellular fractionation and enrichment -- 2.2 Proteolytic digestion for proteomic analysis -- 3. Application of mass spectrometry for the analysis of proteins and peptides -- 3.1 Bottom up/shotgun proteomics -- 3.1.1 Data-dependent acquisition -- 3.1.2 Data-independent acquisition -- 3.2 MS/MS fragmentation strategies -- 3.2.1 Collision-induced dissociation -- 3.2.2 High collision dissociation -- 3.2.3 Neutral loss MS3 -- 3.2.4 Electron transfer dissociation -- 3.2.5 Multistage activation -- 3.3 Identifying peptides from mass spectra -- 3.4 Sequence databases -- 3.5 Mass spectrometry methods for differential proteomics -- 3.5.1 Label-free differential expression proteomics -- 3.5.2 Stable isotopes tags/isobaric mass tags -- 3.6 Mass spectrometry for quantitative targeted proteomics -- 4. Bioinformatic analysis of proteomic data -- 5. Conclusions -- References -- II - Sample preparation -- 3 - Sample preparation for proteomics and mass spectrometry from mammalian cell lines -- 1. Introduction -- 2. Materials and equipment -- 3. Before you begin -- 3.1 Filter-aided sample preparation -- 3.2 PreOmics iST kit -- 3.3 Membrane fractionation -- 3.4 High pH reversed-phase fractionation -- 3.5 Peptide purification and fractionation: strong cation exchange -- 3.6 Tandem Mass Tag (TMT) labeling -- 4. Step-by-step method details -- 4.1 Cell culture and collection of cell pellets for proteomics -- 4.2 Filter-aided sample preparation -- 4.3 PreOmics iST protocol. | |
| 650 | 0 | 7 | |a Massenspektrometrie |0 (DE-588)4037882-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Proteomanalyse |0 (DE-588)4596545-6 |2 gnd |9 rswk-swf |
| 689 | 0 | 0 | |a Proteomanalyse |0 (DE-588)4596545-6 |D s |
| 689 | 0 | 1 | |a Massenspektrometrie |0 (DE-588)4037882-2 |D s |
| 689 | 0 | |5 DE-604 | |
| 700 | 1 | |a Meleady, Paula |0 (DE-588)1133299180 |4 edt | |
| 776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |z 978-0-323-90395-0 |
| 912 | |a ZDB-30-PQE | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-034944687 | ||
| 966 | e | |u https://ebookcentral.proquest.com/lib/munchentech/detail.action?docID=31151346 |l TUM01 |p ZDB-30-PQE |q TUM_Einzelkauf_2024 |x Aggregator |3 Volltext | |
Datensatz im Suchindex
| _version_ | 1804186479486828544 |
|---|---|
| adam_txt | |
| any_adam_object | |
| any_adam_object_boolean | |
| author2 | Meleady, Paula |
| author2_role | edt |
| author2_variant | p m pm |
| author_GND | (DE-588)1133299180 |
| author_facet | Meleady, Paula |
| building | Verbundindex |
| bvnumber | BV049600236 |
| classification_tum | CHE 254 CHE 820 CHE 808 |
| collection | ZDB-30-PQE |
| ctrlnum | (OCoLC)1427317648 (DE-599)KEP100841015 |
| dewey-full | 572.6 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.6 |
| dewey-search | 572.6 |
| dewey-sort | 3572.6 |
| dewey-tens | 570 - Biology |
| discipline | Biologie Chemie |
| discipline_str_mv | Biologie Chemie |
| format | Electronic eBook |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>03657nmm a22004211c 4500</leader><controlfield tag="001">BV049600236</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20240306 </controlfield><controlfield tag="007">cr|uuu---uuuuu</controlfield><controlfield tag="008">240306s2024 |||| o||u| ||||||eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9780323906524</subfield><subfield code="9">978-0-323-90652-4</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)1427317648</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)KEP100841015</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-91G</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">572.6</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">CHE 254</subfield><subfield code="2">stub</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">CHE 820</subfield><subfield code="2">stub</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">CHE 808</subfield><subfield code="2">stub</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Proteomics mass spectrometry methods</subfield><subfield code="b">sample preparation, protein digestion, and research protocols</subfield><subfield code="c">edited by Paula Meleady, National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin, Ireland, School of Biotechnology, Dublin City University, Glasnevin, Dublin, Ireland</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">London ; San Diego ; Cambridge</subfield><subfield code="b">Academic Press, an imprint of Elsevier</subfield><subfield code="c">[2024]</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">1 Online-Ressource</subfield><subfield code="b">Illustrationen, Diagramme</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Description based on publisher supplied metadata and other sources</subfield></datafield><datafield tag="520" ind1="3" ind2=" "><subfield code="a">Front Cover -- Proteomics Mass Spectrometry Methods -- Front Matter -- Copyright -- Contents -- Contributors -- I - Introduction -- 1 - Book introduction -- References -- 2 - Introduction to sample preparation for proteomics and mass spectrometry -- 1. Introduction -- 2. Sample preparation for proteomic analysis -- 2.1 Sample fractionation -- 2.1.1 Immunodepletion for clinical proteomics -- 2.1.2 Subcellular fractionation and enrichment -- 2.2 Proteolytic digestion for proteomic analysis -- 3. Application of mass spectrometry for the analysis of proteins and peptides -- 3.1 Bottom up/shotgun proteomics -- 3.1.1 Data-dependent acquisition -- 3.1.2 Data-independent acquisition -- 3.2 MS/MS fragmentation strategies -- 3.2.1 Collision-induced dissociation -- 3.2.2 High collision dissociation -- 3.2.3 Neutral loss MS3 -- 3.2.4 Electron transfer dissociation -- 3.2.5 Multistage activation -- 3.3 Identifying peptides from mass spectra -- 3.4 Sequence databases -- 3.5 Mass spectrometry methods for differential proteomics -- 3.5.1 Label-free differential expression proteomics -- 3.5.2 Stable isotopes tags/isobaric mass tags -- 3.6 Mass spectrometry for quantitative targeted proteomics -- 4. Bioinformatic analysis of proteomic data -- 5. Conclusions -- References -- II - Sample preparation -- 3 - Sample preparation for proteomics and mass spectrometry from mammalian cell lines -- 1. Introduction -- 2. Materials and equipment -- 3. Before you begin -- 3.1 Filter-aided sample preparation -- 3.2 PreOmics iST kit -- 3.3 Membrane fractionation -- 3.4 High pH reversed-phase fractionation -- 3.5 Peptide purification and fractionation: strong cation exchange -- 3.6 Tandem Mass Tag (TMT) labeling -- 4. Step-by-step method details -- 4.1 Cell culture and collection of cell pellets for proteomics -- 4.2 Filter-aided sample preparation -- 4.3 PreOmics iST protocol.</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Massenspektrometrie</subfield><subfield code="0">(DE-588)4037882-2</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Proteomanalyse</subfield><subfield code="0">(DE-588)4596545-6</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Proteomanalyse</subfield><subfield code="0">(DE-588)4596545-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Massenspektrometrie</subfield><subfield code="0">(DE-588)4037882-2</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Meleady, Paula</subfield><subfield code="0">(DE-588)1133299180</subfield><subfield code="4">edt</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Druck-Ausgabe</subfield><subfield code="z">978-0-323-90395-0</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-30-PQE</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-034944687</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://ebookcentral.proquest.com/lib/munchentech/detail.action?docID=31151346</subfield><subfield code="l">TUM01</subfield><subfield code="p">ZDB-30-PQE</subfield><subfield code="q">TUM_Einzelkauf_2024</subfield><subfield code="x">Aggregator</subfield><subfield code="3">Volltext</subfield></datafield></record></collection> |
| id | DE-604.BV049600236 |
| illustrated | Not Illustrated |
| index_date | 2024-07-03T23:34:36Z |
| indexdate | 2024-07-10T10:11:47Z |
| institution | BVB |
| isbn | 9780323906524 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-034944687 |
| oclc_num | 1427317648 |
| open_access_boolean | |
| owner | DE-91G DE-BY-TUM |
| owner_facet | DE-91G DE-BY-TUM |
| physical | 1 Online-Ressource Illustrationen, Diagramme |
| psigel | ZDB-30-PQE ZDB-30-PQE TUM_Einzelkauf_2024 |
| publishDate | 2024 |
| publishDateSearch | 2024 |
| publishDateSort | 2024 |
| publisher | Academic Press, an imprint of Elsevier |
| record_format | marc |
| spelling | Proteomics mass spectrometry methods sample preparation, protein digestion, and research protocols edited by Paula Meleady, National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin, Ireland, School of Biotechnology, Dublin City University, Glasnevin, Dublin, Ireland London ; San Diego ; Cambridge Academic Press, an imprint of Elsevier [2024] 1 Online-Ressource Illustrationen, Diagramme txt rdacontent c rdamedia cr rdacarrier Description based on publisher supplied metadata and other sources Front Cover -- Proteomics Mass Spectrometry Methods -- Front Matter -- Copyright -- Contents -- Contributors -- I - Introduction -- 1 - Book introduction -- References -- 2 - Introduction to sample preparation for proteomics and mass spectrometry -- 1. Introduction -- 2. Sample preparation for proteomic analysis -- 2.1 Sample fractionation -- 2.1.1 Immunodepletion for clinical proteomics -- 2.1.2 Subcellular fractionation and enrichment -- 2.2 Proteolytic digestion for proteomic analysis -- 3. Application of mass spectrometry for the analysis of proteins and peptides -- 3.1 Bottom up/shotgun proteomics -- 3.1.1 Data-dependent acquisition -- 3.1.2 Data-independent acquisition -- 3.2 MS/MS fragmentation strategies -- 3.2.1 Collision-induced dissociation -- 3.2.2 High collision dissociation -- 3.2.3 Neutral loss MS3 -- 3.2.4 Electron transfer dissociation -- 3.2.5 Multistage activation -- 3.3 Identifying peptides from mass spectra -- 3.4 Sequence databases -- 3.5 Mass spectrometry methods for differential proteomics -- 3.5.1 Label-free differential expression proteomics -- 3.5.2 Stable isotopes tags/isobaric mass tags -- 3.6 Mass spectrometry for quantitative targeted proteomics -- 4. Bioinformatic analysis of proteomic data -- 5. Conclusions -- References -- II - Sample preparation -- 3 - Sample preparation for proteomics and mass spectrometry from mammalian cell lines -- 1. Introduction -- 2. Materials and equipment -- 3. Before you begin -- 3.1 Filter-aided sample preparation -- 3.2 PreOmics iST kit -- 3.3 Membrane fractionation -- 3.4 High pH reversed-phase fractionation -- 3.5 Peptide purification and fractionation: strong cation exchange -- 3.6 Tandem Mass Tag (TMT) labeling -- 4. Step-by-step method details -- 4.1 Cell culture and collection of cell pellets for proteomics -- 4.2 Filter-aided sample preparation -- 4.3 PreOmics iST protocol. Massenspektrometrie (DE-588)4037882-2 gnd rswk-swf Proteomanalyse (DE-588)4596545-6 gnd rswk-swf Proteomanalyse (DE-588)4596545-6 s Massenspektrometrie (DE-588)4037882-2 s DE-604 Meleady, Paula (DE-588)1133299180 edt Erscheint auch als Druck-Ausgabe 978-0-323-90395-0 |
| spellingShingle | Proteomics mass spectrometry methods sample preparation, protein digestion, and research protocols Massenspektrometrie (DE-588)4037882-2 gnd Proteomanalyse (DE-588)4596545-6 gnd |
| subject_GND | (DE-588)4037882-2 (DE-588)4596545-6 |
| title | Proteomics mass spectrometry methods sample preparation, protein digestion, and research protocols |
| title_auth | Proteomics mass spectrometry methods sample preparation, protein digestion, and research protocols |
| title_exact_search | Proteomics mass spectrometry methods sample preparation, protein digestion, and research protocols |
| title_exact_search_txtP | Proteomics mass spectrometry methods sample preparation, protein digestion, and research protocols |
| title_full | Proteomics mass spectrometry methods sample preparation, protein digestion, and research protocols edited by Paula Meleady, National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin, Ireland, School of Biotechnology, Dublin City University, Glasnevin, Dublin, Ireland |
| title_fullStr | Proteomics mass spectrometry methods sample preparation, protein digestion, and research protocols edited by Paula Meleady, National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin, Ireland, School of Biotechnology, Dublin City University, Glasnevin, Dublin, Ireland |
| title_full_unstemmed | Proteomics mass spectrometry methods sample preparation, protein digestion, and research protocols edited by Paula Meleady, National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin, Ireland, School of Biotechnology, Dublin City University, Glasnevin, Dublin, Ireland |
| title_short | Proteomics mass spectrometry methods |
| title_sort | proteomics mass spectrometry methods sample preparation protein digestion and research protocols |
| title_sub | sample preparation, protein digestion, and research protocols |
| topic | Massenspektrometrie (DE-588)4037882-2 gnd Proteomanalyse (DE-588)4596545-6 gnd |
| topic_facet | Massenspektrometrie Proteomanalyse |
| work_keys_str_mv | AT meleadypaula proteomicsmassspectrometrymethodssamplepreparationproteindigestionandresearchprotocols |