Exploring and exploiting yeast volatile metabolites:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Aachen
Apprimus Verlag
2023
|
| Ausgabe: | 1. Auflage |
| Schriftenreihe: | Applied Microbiology
Volume 30 |
| Schlagworte: | |
| Online-Zugang: | Volltext Inhaltsverzeichnis |
| Beschreibung: | XVI, 161 Seiten Illustrationen, Diagramme 21 cm x 14.8 cm, 285 g |
| ISBN: | 9783985551507 3985551502 |
| DOI: | 10.18154/RWTH-2023-02767 |
Internformat
MARC
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| 100 | 1 | |a Mengers, Hendrik G. |d 1995- |e Verfasser |0 (DE-588)1284125009 |4 aut | |
| 245 | 1 | 0 | |a Exploring and exploiting yeast volatile metabolites |c Hendrik Gerhard Mengers |
| 250 | |a 1. Auflage | ||
| 264 | 1 | |a Aachen |b Apprimus Verlag |c 2023 | |
| 300 | |a XVI, 161 Seiten |b Illustrationen, Diagramme |c 21 cm x 14.8 cm, 285 g | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Applied Microbiology |v Volume 30 | |
| 502 | |b Dissertation |c RWTH Aachen University |d 2023 | ||
| 650 | 0 | 7 | |a Saccharomyces cerevisiae |0 (DE-588)4178812-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Flüchtiger Stoff |0 (DE-588)4281393-1 |2 gnd |9 rswk-swf |
| 653 | |a Mikrobiologie | ||
| 653 | |a Naturwissenschaften | ||
| 653 | |a Massenspektometrie | ||
| 653 | |a Hefe | ||
| 653 | |a volatile Stoffe | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Saccharomyces cerevisiae |0 (DE-588)4178812-6 |D s |
| 689 | 0 | 1 | |a Flüchtiger Stoff |0 (DE-588)4281393-1 |D s |
| 689 | 0 | |5 DE-604 | |
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| 776 | 0 | 8 | |i Erscheint auch als |n Online-Ausgabe, PDF |o https://doi.org/10.18154/RWTH-2023-02767 |
| 830 | 0 | |a Applied Microbiology |v Volume 30 |w (DE-604)BV042644516 |9 30 | |
| 856 | 4 | 1 | |u https://doi.org/10.18154/RWTH-2023-02767 |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=034248765&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 912 | |a ebook | ||
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Datensatz im Suchindex
| _version_ | 1804185237280784384 |
|---|---|
| adam_text | TABLE
OF
CONTENTS
TABLE
OF
CONTENTS
TABLE
OF
CONTENTS
....................................................................................
I
SUMMARY
...................................................................................................................V
ZUSAMMENFASSUNG
.................................................................................................
VII
LIST
OF
ABBREVIATIONS
.................................................................................................
IX
LIST
OF
FIGURES
.........................................................................................................
XIII
LIST
OF
TABLES
..........................................................................................................
XIV
LIST
OF
EQUATIONS
.....................................................................................................
XV
1
GENERAL
INTRODUCTION
...........................................................................................
3
1.1
ANTHROPOGENIC
CO2
CAUSES
CLIMATE
CHANGE
...................................................
3
1.2
BIOTECHNOLOGY
HAS
A
KEY
ROLE
IN
MINIMISING
CO2
EMISSIONS
.........................
4
1.3
MOLECULAR
BASIS
OF
VOLATILITY
28-30
....................................................................
4
1.4
VOLATILE
ORGANIC
COMPOUNDS
AND
THE
SMELL
OF
YEAST
.......................................
6
1.4.1
VOCS
DERIVED
FROM
S.
CEREVISIAE
S
CENTRAL
CARBON
METABOLISM
.......
6
1.4.2
VOCS
DERIVED
FROM
S.
CEREVISIAE S
AMINO
ACID
METABOLISM
............
8
1.4.3
VOCS
DERIVED
FROM
OTHER
S.
CEREVISIAE
PATHWAYS
.........................
10
1.5
ANALYTICAL
METHODS
FOR
EXPLORING
VOLATILE
METABOLITES
OF
S.
CEREVISIAE
.......
10
1.5.1
GAS
CHROMATOGRAPHY-BASED
METHODS
............................................
11
1.5.2
ION
MOBILITY
SPECTROMETRY
(IMS)
......................................................
11
1.5.3
MASS
SPECTROMETRY-BASED
METHODS
...............................................
12
1.6
THE
EXPLOITATION
OF
S.
CEREVISIAE
VOLATILES
....................................................
15
1.6.1
BIOFUEL
PRODUCTION
..........................................................................
15
1.6.2
FLAVOUR
VOLATILES
.............................................................................
16
1.6.3
OTHER
APPLICATIONS
..........................................................................
17
1.7
SCOPE
AND
OUTLINE
OF
THIS
THESIS
.................................................................
17
2
MATERIAL
AND
METHODS
........................................................................................
21
2.1
CHEMICALS
AND
PLANTS
.................................................................................
21
2.2
STRAINS
........................................................................................................
21
2.3
MEDIA
.........................................................................................................
21
2.4
CULTIVATION
PROCEDURES................................................................................
22
2.4.1
YEAST
CULTIVATION
FOR
IN
SITU
CO2
HYDROGENATION
..............................
22
2.4.2
YEAST
CULTIVATION
FOR
ACETALDEHYDE
PRODUCTION
IN
200
ML
SCALE
....
23
2.4.3
YEAST
CULTIVATION
FOR
ACETALDEHYDE
PRODUCTION
IN
HUNGATE
TUBES...
23
2.4.4
YEAST
CULTIVATION
FOR
ACETALDEHYDE
PRODUCTION
IN
750
ML
SCALE
.....
23
2.4.5
YEAST
CULTIVATION
FOR
SESI-ORBITRAP
MS
VOLATILOME
ANALYSIS
.........
24
2.5
SESI-ORBITRAP
MS.......................................................................................
25
I
TABLE
OF
CONTENTS
2.5.1
SESI-ORBITRAP
MS
FOR
MEASURING
GARLIC-DERIVED
VOLATILE
SULFUR
COMPOUNDS
.....................................................................................
26
2.5.2
SESI-ORBITRAP
MS
FOR
MEASURING
THE
YEAST
VOLATILOME
.................
28
2.5.3
ANALYSIS
OF
NATURAL
ISOTOPE
PEAKS
USING
SESI-ORBITRAP
MS
..........
29
2.6
ANALYTICAL
METHODS
......................................................................................
30
2.6.1
DETERMINATION
OF
MICROBIAL
GROWTH
...................................................
30
2.6.2
METABOLITE
QUANTIFICATION
IN
THE
LIQUID
PHASE
..................................
31
2.6.3
DETERMINATION
OF
HENRY
COEFFICIENTS
................................................
31
3
RESULTS
.................................................................................................................
37
3.1
BIO-ENERGY
CONVERSION
WITH
CARBON
CAPTURE
AND
UTILIZATION:
INTEGRATED
BIOMASS
FERMENTATION
AND
CHEMO-CATALYTIC
CO2
HYDROGENATION
FOR
BIO
ETHANOL
AND
FORMIC
ACID
CO-PRODUCTION
................................................
37
3.1.1
ABSTRACT
...........................................................................................
37
3.1.2
INTRODUCTION
......................................................................................
37
3.1.3
RESULTS
AND
DISCUSSION
..................................................................
39
3.1.4
CONCLUSION
......................................................................................
45
3.2
YEAST-BASED
PRODUCTION
AND
IN
SITU
PURIFICATION
OF
ACETALDEHYDE
49
3.2.1
ABSTRACT
........................................................................................
49
3.2.2
INTRODUCTION
......................................................................................
49
3.2.3
RESULTS
AND
DISCUSSION
..................................................................
51
3.2.4
CONCLUSION
......................................................................................
60
3.3
SEEING
THE
SMELL
OF
GARLIC:
DETECTION
OF
GAS
PHASE
VOLATILES
FROM
CRUSHED
GARLIC,
ONION,
RAMSONS
AND
HUMAN
GARLIC
BREATH
USING
SESI
ORBITRAP
MS
.................................................................................................
65
3.3.1
ABSTRACT
...........................................................................................
65
3.3.2
INTRODUCTION
.....................................................................................
65
3.3.3
RESULTS
AND
DISCUSSION
..................................................................
69
3.3.4
CONCLUSION
......................................................................................
79
3.4
USING
OFF-GAS
FOR
INSIGHTS:
ONLINE
MONITORING
OF
ETHANOL
AND
YEAST
VOLATILOME
USING
SESI-ORBITRAP
MS
.............................................................
83
3.4.1
ABSTRACT
...........................................................................................
83
3.4.2
INTRODUCTION
.....................................................................................
83
3.4.3
RESULTS
AND
DISCUSSION
..................................................................
85
3.4.4
CONCLUSION
......................................................................................
95
4
GENERAL
DISCUSSION
AND
OUTLOOK
..............................................
99
4.1
ANALYSING
VOLATILES
......................................................................................
99
4.2
PRODUCING
VOLATILES
....................................................................................
102
4.3
DEALING
WITH
NON-WANTED
VOLATILES
..............................................................
105
4.4
CONCLUSION
AND
OUTLOOK
...........................................................................
107
5
APPENDIX
...........................................................................................................
111
5.1
SUPPLEMENTARY
MATERIAL
AND
METHODS
......................................................
111
II
TABLE
OF
CONTENTS
5.2
SUPPLEMENTARY
RESULTS
............................................................................
115
5.2.1
SUPPLEMENTARY
FOR
CHAPTER
3.1
.....................................................
115
5.2.2
SUPPLEMENTARY
FOR
CHAPTER
3.2
.....................................................
121
5.2.3
SUPPLEMENTARY
FOR
CHAPTER
3.3
.....................................................
125
5.2.4
SUPPLEMENTARY
FOR
CHAPTER
3.4
.....................................................
128
5.3
SUPPLEMENTARY
GENERAL
DISCUSSION
AND
OUTLOOK
.....................................
131
6
REFERENCES
......................................................................................................
137
7
CURRICULUM
VITAE
.............................................................................................
159
III
|
| adam_txt |
TABLE
OF
CONTENTS
TABLE
OF
CONTENTS
TABLE
OF
CONTENTS
.
I
SUMMARY
.V
ZUSAMMENFASSUNG
.
VII
LIST
OF
ABBREVIATIONS
.
IX
LIST
OF
FIGURES
.
XIII
LIST
OF
TABLES
.
XIV
LIST
OF
EQUATIONS
.
XV
1
GENERAL
INTRODUCTION
.
3
1.1
ANTHROPOGENIC
CO2
CAUSES
CLIMATE
CHANGE
.
3
1.2
BIOTECHNOLOGY
HAS
A
KEY
ROLE
IN
MINIMISING
CO2
EMISSIONS
.
4
1.3
MOLECULAR
BASIS
OF
VOLATILITY
28-30
.
4
1.4
VOLATILE
ORGANIC
COMPOUNDS
AND
THE
SMELL
OF
YEAST
.
6
1.4.1
VOCS
DERIVED
FROM
S.
CEREVISIAE
'
S
CENTRAL
CARBON
METABOLISM
.
6
1.4.2
VOCS
DERIVED
FROM
S.
CEREVISIAE'S
AMINO
ACID
METABOLISM
.
8
1.4.3
VOCS
DERIVED
FROM
OTHER
S.
CEREVISIAE
PATHWAYS
.
10
1.5
ANALYTICAL
METHODS
FOR
EXPLORING
VOLATILE
METABOLITES
OF
S.
CEREVISIAE
.
10
1.5.1
GAS
CHROMATOGRAPHY-BASED
METHODS
.
11
1.5.2
ION
MOBILITY
SPECTROMETRY
(IMS)
.
11
1.5.3
MASS
SPECTROMETRY-BASED
METHODS
.
12
1.6
THE
EXPLOITATION
OF
S.
CEREVISIAE
VOLATILES
.
15
1.6.1
BIOFUEL
PRODUCTION
.
15
1.6.2
FLAVOUR
VOLATILES
.
16
1.6.3
OTHER
APPLICATIONS
.
17
1.7
SCOPE
AND
OUTLINE
OF
THIS
THESIS
.
17
2
MATERIAL
AND
METHODS
.
21
2.1
CHEMICALS
AND
PLANTS
.
21
2.2
STRAINS
.
21
2.3
MEDIA
.
21
2.4
CULTIVATION
PROCEDURES.
22
2.4.1
YEAST
CULTIVATION
FOR
IN
SITU
CO2
HYDROGENATION
.
22
2.4.2
YEAST
CULTIVATION
FOR
ACETALDEHYDE
PRODUCTION
IN
200
ML
SCALE
.
23
2.4.3
YEAST
CULTIVATION
FOR
ACETALDEHYDE
PRODUCTION
IN
HUNGATE
TUBES.
23
2.4.4
YEAST
CULTIVATION
FOR
ACETALDEHYDE
PRODUCTION
IN
750
ML
SCALE
.
23
2.4.5
YEAST
CULTIVATION
FOR
SESI-ORBITRAP
MS
VOLATILOME
ANALYSIS
.
24
2.5
SESI-ORBITRAP
MS.
25
I
TABLE
OF
CONTENTS
2.5.1
SESI-ORBITRAP
MS
FOR
MEASURING
GARLIC-DERIVED
VOLATILE
SULFUR
COMPOUNDS
.
26
2.5.2
SESI-ORBITRAP
MS
FOR
MEASURING
THE
YEAST
VOLATILOME
.
28
2.5.3
ANALYSIS
OF
NATURAL
ISOTOPE
PEAKS
USING
SESI-ORBITRAP
MS
.
29
2.6
ANALYTICAL
METHODS
.
30
2.6.1
DETERMINATION
OF
MICROBIAL
GROWTH
.
30
2.6.2
METABOLITE
QUANTIFICATION
IN
THE
LIQUID
PHASE
.
31
2.6.3
DETERMINATION
OF
HENRY
COEFFICIENTS
.
31
3
RESULTS
.
37
3.1
BIO-ENERGY
CONVERSION
WITH
CARBON
CAPTURE
AND
UTILIZATION:
INTEGRATED
BIOMASS
FERMENTATION
AND
CHEMO-CATALYTIC
CO2
HYDROGENATION
FOR
BIO
ETHANOL
AND
FORMIC
ACID
CO-PRODUCTION
.
37
3.1.1
ABSTRACT
.
37
3.1.2
INTRODUCTION
.
37
3.1.3
RESULTS
AND
DISCUSSION
.
39
3.1.4
CONCLUSION
.
45
3.2
YEAST-BASED
PRODUCTION
AND
IN
SITU
PURIFICATION
OF
ACETALDEHYDE
49
3.2.1
ABSTRACT
.
49
3.2.2
INTRODUCTION
.
49
3.2.3
RESULTS
AND
DISCUSSION
.
51
3.2.4
CONCLUSION
.
60
3.3
SEEING
THE
SMELL
OF
GARLIC:
DETECTION
OF
GAS
PHASE
VOLATILES
FROM
CRUSHED
GARLIC,
ONION,
RAMSONS
AND
HUMAN
GARLIC
BREATH
USING
SESI
ORBITRAP
MS
.
65
3.3.1
ABSTRACT
.
65
3.3.2
INTRODUCTION
.
65
3.3.3
RESULTS
AND
DISCUSSION
.
69
3.3.4
CONCLUSION
.
79
3.4
USING
OFF-GAS
FOR
INSIGHTS:
ONLINE
MONITORING
OF
ETHANOL
AND
YEAST
VOLATILOME
USING
SESI-ORBITRAP
MS
.
83
3.4.1
ABSTRACT
.
83
3.4.2
INTRODUCTION
.
83
3.4.3
RESULTS
AND
DISCUSSION
.
85
3.4.4
CONCLUSION
.
95
4
GENERAL
DISCUSSION
AND
OUTLOOK
.
99
4.1
ANALYSING
VOLATILES
.
99
4.2
PRODUCING
VOLATILES
.
102
4.3
DEALING
WITH
NON-WANTED
VOLATILES
.
105
4.4
CONCLUSION
AND
OUTLOOK
.
107
5
APPENDIX
.
111
5.1
SUPPLEMENTARY
MATERIAL
AND
METHODS
.
111
II
TABLE
OF
CONTENTS
5.2
SUPPLEMENTARY
RESULTS
.
115
5.2.1
SUPPLEMENTARY
FOR
CHAPTER
3.1
.
115
5.2.2
SUPPLEMENTARY
FOR
CHAPTER
3.2
.
121
5.2.3
SUPPLEMENTARY
FOR
CHAPTER
3.3
.
125
5.2.4
SUPPLEMENTARY
FOR
CHAPTER
3.4
.
128
5.3
SUPPLEMENTARY
GENERAL
DISCUSSION
AND
OUTLOOK
.
131
6
REFERENCES
.
137
7
CURRICULUM
VITAE
.
159
III |
| any_adam_object | 1 |
| any_adam_object_boolean | 1 |
| author | Mengers, Hendrik G. 1995- |
| author_GND | (DE-588)1284125009 |
| author_facet | Mengers, Hendrik G. 1995- |
| author_role | aut |
| author_sort | Mengers, Hendrik G. 1995- |
| author_variant | h g m hg hgm |
| building | Verbundindex |
| bvnumber | BV048985332 |
| collection | ebook |
| ctrlnum | (OCoLC)1375675697 (DE-599)DNB1283633531 |
| doi_str_mv | 10.18154/RWTH-2023-02767 |
| edition | 1. Auflage |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV048985332 |
| illustrated | Illustrated |
| index_date | 2024-07-03T22:05:55Z |
| indexdate | 2024-07-10T09:52:02Z |
| institution | BVB |
| institution_GND | (DE-588)1068101474 |
| isbn | 9783985551507 3985551502 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-034248765 |
| oclc_num | 1375675697 |
| open_access_boolean | 1 |
| owner | DE-29T |
| owner_facet | DE-29T |
| physical | XVI, 161 Seiten Illustrationen, Diagramme 21 cm x 14.8 cm, 285 g |
| psigel | ebook |
| publishDate | 2023 |
| publishDateSearch | 2023 |
| publishDateSort | 2023 |
| publisher | Apprimus Verlag |
| record_format | marc |
| series | Applied Microbiology |
| series2 | Applied Microbiology |
| spelling | Mengers, Hendrik G. 1995- Verfasser (DE-588)1284125009 aut Exploring and exploiting yeast volatile metabolites Hendrik Gerhard Mengers 1. Auflage Aachen Apprimus Verlag 2023 XVI, 161 Seiten Illustrationen, Diagramme 21 cm x 14.8 cm, 285 g txt rdacontent n rdamedia nc rdacarrier Applied Microbiology Volume 30 Dissertation RWTH Aachen University 2023 Saccharomyces cerevisiae (DE-588)4178812-6 gnd rswk-swf Flüchtiger Stoff (DE-588)4281393-1 gnd rswk-swf Mikrobiologie Naturwissenschaften Massenspektometrie Hefe volatile Stoffe (DE-588)4113937-9 Hochschulschrift gnd-content Saccharomyces cerevisiae (DE-588)4178812-6 s Flüchtiger Stoff (DE-588)4281393-1 s DE-604 Apprimus Verlag (DE-588)1068101474 pbl Erscheint auch als Online-Ausgabe, PDF https://doi.org/10.18154/RWTH-2023-02767 Applied Microbiology Volume 30 (DE-604)BV042644516 30 https://doi.org/10.18154/RWTH-2023-02767 Resolving-System kostenfrei Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=034248765&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p vlb 20230316 DE-101 https://d-nb.info/provenance/plan#vlb |
| spellingShingle | Mengers, Hendrik G. 1995- Exploring and exploiting yeast volatile metabolites Applied Microbiology Saccharomyces cerevisiae (DE-588)4178812-6 gnd Flüchtiger Stoff (DE-588)4281393-1 gnd |
| subject_GND | (DE-588)4178812-6 (DE-588)4281393-1 (DE-588)4113937-9 |
| title | Exploring and exploiting yeast volatile metabolites |
| title_auth | Exploring and exploiting yeast volatile metabolites |
| title_exact_search | Exploring and exploiting yeast volatile metabolites |
| title_exact_search_txtP | Exploring and exploiting yeast volatile metabolites |
| title_full | Exploring and exploiting yeast volatile metabolites Hendrik Gerhard Mengers |
| title_fullStr | Exploring and exploiting yeast volatile metabolites Hendrik Gerhard Mengers |
| title_full_unstemmed | Exploring and exploiting yeast volatile metabolites Hendrik Gerhard Mengers |
| title_short | Exploring and exploiting yeast volatile metabolites |
| title_sort | exploring and exploiting yeast volatile metabolites |
| topic | Saccharomyces cerevisiae (DE-588)4178812-6 gnd Flüchtiger Stoff (DE-588)4281393-1 gnd |
| topic_facet | Saccharomyces cerevisiae Flüchtiger Stoff Hochschulschrift |
| url | https://doi.org/10.18154/RWTH-2023-02767 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=034248765&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV042644516 |
| work_keys_str_mv | AT mengershendrikg exploringandexploitingyeastvolatilemetabolites AT apprimusverlag exploringandexploitingyeastvolatilemetabolites |