Production and characterization of novel BMMF2 rep-specific antibodies and application in cancer histology:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Heidelberg
2022
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Physical Description: | 148 Seiten Illustrationen, Diagramme 31 cm |
Staff View
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV048961336 | ||
| 003 | DE-604 | ||
| 007 | t| | ||
| 008 | 230515s2022 gw a||| m||| 00||| eng d | ||
| 015 | |a 23,H01 |2 dnb | ||
| 016 | 7 | |a 1274880688 |2 DE-101 | |
| 020 | |c Festeinband | ||
| 035 | |a (OCoLC)1352255158 | ||
| 035 | |a (DE-599)KXP1824053452 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 044 | |a gw |c XA-DE | ||
| 049 | |a DE-355 | ||
| 084 | |8 1\p |a 616.994 |2 23ksdnb | ||
| 084 | |a 610 |2 sdnb | ||
| 084 | |8 2\p |a 610 |2 23sdnb | ||
| 100 | 1 | |a Beck, Simon |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Production and characterization of novel BMMF2 rep-specific antibodies and application in cancer histology |c presented by M. Sc. Simon Beck |
| 264 | 1 | |a Heidelberg |c 2022 | |
| 300 | |a 148 Seiten |b Illustrationen, Diagramme |c 31 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Ruprechts-Karls-University Heidelberg |d 2022 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 700 | 1 | |a Bartenschlager, Ralf |d 1958- |0 (DE-588)1058097989 |4 dgs | |
| 700 | 1 | |a Zur Hausen, Harald |d 1936-2023 |0 (DE-588)121279731 |4 dgs | |
| 710 | 2 | |a Universität Heidelberg |0 (DE-588)2024349-2 |4 dgg | |
| 856 | 4 | 2 | |m B:DE-101 |q application/pdf |u https://d-nb.info/1274880688/04 |3 Inhaltsverzeichnis |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=034225090&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 883 | 0 | |8 1\p |a emakn |c 0,56841 |d 20221213 |q DE-101 |u https://d-nb.info/provenance/plan#emakn | |
| 883 | 2 | |8 2\p |a dnb |d 20221214 |q DE-101 |u https://d-nb.info/provenance/plan#dnb | |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-034225090 | |
Record in the Search Index
| _version_ | 1822127863041622016 |
|---|---|
| adam_text |
INDEX
1.
INTRODUCTION
.
11
1.1.
CANCER
-
BACKGROUND
AND
ITS
LINK
TO
INFECTIOUS
AGENTS
.
11
1.2.
BOVINE
MEAT
AND
MILK
FACTORS
(BMMF)
.
13
1.2.1.
EPIDEMIOLOGY,
ORIGIN
AND
ISOLATION
OF
BMMF
.
13
1.2.2.
FUNCTIONAL
FEATURES
OF
BMMF
.
16
1.2.3.
THE
BMMF
REPLICATION
INITIATOR
PROTEIN
(REP)
.
16
1.2.4.
BMMF1
LINKED
TO
INDIRECT
CARCINOGENESIS
.
18
1.3.
EPIDEMIOLOGY,
ETIOLOGY,
AND
MICROBIOME
OF
COLORECTAL
CANCER
(CRC)
.
19
1.3.1.
BMMF
AS
RISK
FACTOR
FOR
OTHER
CANCERS
.
21
1.3.2. LIVER
HEPATOCELLULAR
CARCINOMA
(HCC)
.
22
1.3.3.
LUNG
CANCER
(NSCLC/SCLC)
.
23
1.4.
AIM
OF
THIS
STUDY
.
25
2.
MATERIALS
&
METHODS
.
26
2.1.
MATERIALS
-
OVERVIEW
OF
REAGENTS,
BUFFERS,
DEVICES
AND
SOFTWARE
.
26
2.2.
METHODS
.
32
2.2.1.
IN
SILICO
(PHYLOGENY)
.
32
2.2.2.
CLONING
OF
REP
EXPRESSION
PLASMIDS
.
33
2.2.2.I.
STANDARD
PCR
FOR
DNA
CLONING
.
33
2.2.2.2.
DNA
GEL
EXTRACTION
AND
PCR
CLEAN-UP
.
33
2.2.2.3.
LIGATION
OF
DNA
FRAGMENTS
.
34
2.2.2.4.
TRANSFORMATION
OF
DNA
INTO
E.COLI
.
34
2.2.2.5.
PLASMID
DNA
ISOLATION
FROM
E.
COLI
CELLS
.
34
2.2.2.6.
RESTRICTION
DIGEST
AND
SEQUENCING
OF
DNA
.
34
2.2.3.
DNA
TRANSFECTION
OF
IN
VITRO
CELL
CULTURE
.
35
2.2.4.
CELL
CULTIVATION
.
35
2.2.5.
DNA
EXTRACTION
FROM
IN
VITRO
CELL
CULTURE
.
36
2.2.6.
IN
VITRO
VIABILITY
ASSAYS
.
36
2.2.7.
REPLICATION
ASSAY
OF
BMMF2
DNA
IN
HEK293TT
.
37
2.2.7.I.
CIRCULARIZATION
OF
BMMF
GENOMES
FOR
TRANSFECTION
EXPERIMENTS
.
37
2.2.7.2.
METHYLATION
OF
DAM
DNA
.
37
2.2.7.3.
CELL
HARVEST
OF
BIOACTIVITY
STUDIES
ON
DNA,
RNA
AND
PROTEIN
LEVEL
.
37
2.2.7.4.
DPNL-DIGEST
AND
PRECIPITATION
OF
TOTAL
DNA
.
38
2.2.7.5.
TOUCH-DOWN
PCR
OF
BMMF2
DNA
.
38
2.2.7.6.
QUANTITATIVE
PCR
OF
BMMF2
DNA
.
39
2.2.8.
SDS-PAGE
AND
WESTERN
BLOT
.
39
6
2.2.9.
IMMUNODETECTION
.
40
2.2.10.
PURIFICATION
OF
C2MI.1A.2
AND
C2MI.15B.6
REP
PROTEIN
.
41
2.2.11.
PURIFICATION
OF
C2MI.8B.7
REP
PROTEIN
.
2.2.12.
BRADFORD
ASSAY
.
2.2.13.
PRODUCTION
OF
MOUSE
MONOCLONAL
ANTIBODIES
AGAINST
BMMF2
REP
.
42
2.2.14.
ANTIBODY
VALIDATION
BY
ELISA
(CORE
FACILITY
DKF2)
.
42
2.2.15.
ANTIBODY
VALIDATION
BY
WB
AND
IF
MICROSCOPY
.
43
2.2.16.
EPITOPE
MAPPING
(WITH
PEPPERPRINT)
.
43
2.2.17.
IMMUNOPRECIPITATION
.
45
2.2.18.
IMMUNOHISTOCHEMISTRY
.
45
2.2.19.
PREPARATION
OF
TISSUE
AND
CELL
LYSATES
FOR
WB
AND
MASS
SPECTROMETRY
.
46
2.2.20.
MASS
SPECTROMETRY
ANALYSIS
(PROTEOMICS
CORE
FACILITY)
.
47
2.2.21.
LASER
MICRODISSECTION
(LMD)
.
48
2.2.22.
ROLLING-CIRCLE
AMPLIFICATION
(RCA)
.
48
2.2.23.
DIRECT
PCR
AMPLIFICATION
OF
BMMF2
.
48
2.2.24.
IN
SILICO
ANALYSIS
OF
THE
PRESENCE
OF
BMMF
SEQUENCES
IN
CANCER
PATIENTS
.
48
3.
RESULTS
.
50
3.1.
PHYLOGENY
OF
BMMF
AND
IDENTIFYING
REPRESENTATIVE
BMMF2
ISOLATES
SUITABLE
FOR
THE
PRODUCTION
OF
BMMF2
REP-SPECIFIC
ANTIBODIES
.
50
3.2.
BMMF2
IN
VITRO
CHARACTERISTICS
AFTER
TRANSFECTION
.
56
3.2.1.
BMMF2
REPLICATION
IN
CELL
CULTURE
.
56
3.2.2.
CHARACTERIZATION
OF
BMMF2
REP
EXPRESSION
IN
HUMAN
CELL
CULTURE
.
59
3.2.3.
PHENOTYPIC
ANALYSIS
OF
BMMF2
REP
OVEREXPRESSION
IN
HEK293TT
.
61
3.3.
PRODUCTION
AND
SELECTION
OF
BMMF2
REP-SPECIFIC
ANTIBODIES
.
62
3.3.1.
BMMF2
REP
PROTEIN
OVEREXPRESSION
AND
PURIFICATION
.
63
3.3.2.
IMMUNIZATION
STRATEGY
.
64
3.3.3.
SETUP
FOR
ANTIBODY
SCREENING AND
SELECTION
CRITERIA
OF
BMMF2
REP
ANTIBODY
CANDIDATES
65
3.4.
CHARACTERIZATION
OF
BMMF2
REP
ANTIBODIES
.
66
3.4.1.
IDENTIFICATION
OF
ANTIBODY
ISOTYPES
AND
LINEAR
EPITOPES
.
66
3.4.2.
SPECIFICITY
IN
WESTERN
BLOT
.
68
3.4.3.
SPECIFICITY
IN
IMMUNOFLUORESCENCE
MICROSCOPY
.
69
3.4.4.
IMMUNOPRECIPITATION
CAPABILITY
OF
BMMF2
REP
ANTIBODIES
.
69
3.4.5.
IHC
SPECIFICITY
BASED
ON
ANTIBODY
AND
ANTIGEN-DOSE
DEPENDENCY
.
70
3.4.6.
SUMMARY
OF
SELECTED
BMMF2
REP
ANTIBODIES
.
74
3.5.
BMMF2
IN
HUMAN
CANCER
.
75
3.5.1.
BMMF2
REP
EXPRESSION
IN
TISSUES
OF
CANCER
PATIENTS
.
75
7
3.5.2.
AFFIRMATION
OF
IHC
DETECTION
BY
BMMF2
TARGET
COMPETITION
.
82
3.5.3.
WHOLE
TISSUE
LYSIS
AND
SUBSEQUENT
MASS
SPECTROMETRY
ANALYSIS
.
84
3.5.4.
LASER
MICRODISSECTION
OF
PATIENT
TISSUE
FOR
BMMF2-SPECIFIC
PCR
.
85
3.5.5.
OVERVIEW
OF
HUMAN
MATERIAL
EXAMINED
FOR
BMMF2
.
85
3.5.6.
INTRINSIC
BMMF2
REP
PROTEIN
POSITIVITY
OF
CANCER CELL
LINES
.
86
3.5.7.
MASS
SPECTROMETRY
ANALYSIS
OF
BMMF-LIKE
PEPTIDES
FROM
CELL
LINES
.
87
4.
DISCUSSION.
89
4.1.
BMMF
PHYLOGENY
AND
SELECTION
OF
BMMF2
REP
IMMUNOGENS
FOR
PRODUCTION
OF
BMMF2
REP
SPECIFIC
ANTIBODIES
.
89
4.2.
IN
VITRO
BIOACTIVITY
OF
BMMF2
.
92
4.2.1.
BMMF2
REPLICATION
.
92
4.2.2.
BMMF2
REP
OVEREXPRESSION
AND
EFFECT
ON
VIABILITY
.
96
4.3.
BMMF2
REP
PROTEIN
PRODUCTION
AND
SELECTION
OF
BMMF2
REP-SPECIFIC
ANTIBODIES
.
98
4.4.
CHARACTERIZATION
OF
BMMF2
REP
ANTIBODIES
.
100
4.5.
BMMF2
IN
VIVO
.
104
4.5.1.
BMMF2
REP
IN
CANCER
PATIENT
TISSUES
(IHC)
.
104
4.5.2.
WHOLE
TISSUE
LYSIS
AND
SUBSEQUENT
MASS
SPECTROMETRY
ANALYSIS
.
108
4.5.2.
BMMF2
DNA
IN
CANCER
PATIENT
TISSUES
(LMD)
.
109
4.5.3.
ASSESSMENT
OF
BMMF2
ANTIBODY
APPLICABILITY
BASED
ON
IN
SILICO
ANALYSIS
OF
BMMF
IN
CANCER
DATABASES
.
ILL
4.5.3.
INTRINSIC
BMMF2
REP
PROTEIN
POSITIVITY
OF
CANCER CELL
LINES
.
112
5.
CONCLUSION
AND
OUTLOOK
.
115
6.
SUPPLEMENT
.
120
7.
LITERATURE
.
136
ABBREVIATIONS
.
144
ACKNOWLEDGMENTS
.
147 |
| adam_txt |
INDEX
1.
INTRODUCTION
.
11
1.1.
CANCER
-
BACKGROUND
AND
ITS
LINK
TO
INFECTIOUS
AGENTS
.
11
1.2.
BOVINE
MEAT
AND
MILK
FACTORS
(BMMF)
.
13
1.2.1.
EPIDEMIOLOGY,
ORIGIN
AND
ISOLATION
OF
BMMF
.
13
1.2.2.
FUNCTIONAL
FEATURES
OF
BMMF
.
16
1.2.3.
THE
BMMF
REPLICATION
INITIATOR
PROTEIN
(REP)
.
16
1.2.4.
BMMF1
LINKED
TO
INDIRECT
CARCINOGENESIS
.
18
1.3.
EPIDEMIOLOGY,
ETIOLOGY,
AND
MICROBIOME
OF
COLORECTAL
CANCER
(CRC)
.
19
1.3.1.
BMMF
AS
RISK
FACTOR
FOR
OTHER
CANCERS
.
21
1.3.2. LIVER
HEPATOCELLULAR
CARCINOMA
(HCC)
.
22
1.3.3.
LUNG
CANCER
(NSCLC/SCLC)
.
23
1.4.
AIM
OF
THIS
STUDY
.
25
2.
MATERIALS
&
METHODS
.
26
2.1.
MATERIALS
-
OVERVIEW
OF
REAGENTS,
BUFFERS,
DEVICES
AND
SOFTWARE
.
26
2.2.
METHODS
.
32
2.2.1.
IN
SILICO
(PHYLOGENY)
.
32
2.2.2.
CLONING
OF
REP
EXPRESSION
PLASMIDS
.
33
2.2.2.I.
STANDARD
PCR
FOR
DNA
CLONING
.
33
2.2.2.2.
DNA
GEL
EXTRACTION
AND
PCR
CLEAN-UP
.
33
2.2.2.3.
LIGATION
OF
DNA
FRAGMENTS
.
34
2.2.2.4.
TRANSFORMATION
OF
DNA
INTO
E.COLI
.
34
2.2.2.5.
PLASMID
DNA
ISOLATION
FROM
E.
COLI
CELLS
.
34
2.2.2.6.
RESTRICTION
DIGEST
AND
SEQUENCING
OF
DNA
.
34
2.2.3.
DNA
TRANSFECTION
OF
IN
VITRO
CELL
CULTURE
.
35
2.2.4.
CELL
CULTIVATION
.
35
2.2.5.
DNA
EXTRACTION
FROM
IN
VITRO
CELL
CULTURE
.
36
2.2.6.
IN
VITRO
VIABILITY
ASSAYS
.
36
2.2.7.
REPLICATION
ASSAY
OF
BMMF2
DNA
IN
HEK293TT
.
37
2.2.7.I.
CIRCULARIZATION
OF
BMMF
GENOMES
FOR
TRANSFECTION
EXPERIMENTS
.
37
2.2.7.2.
METHYLATION
OF
DAM
DNA
.
37
2.2.7.3.
CELL
HARVEST
OF
BIOACTIVITY
STUDIES
ON
DNA,
RNA
AND
PROTEIN
LEVEL
.
37
2.2.7.4.
DPNL-DIGEST
AND
PRECIPITATION
OF
TOTAL
DNA
.
38
2.2.7.5.
TOUCH-DOWN
PCR
OF
BMMF2
DNA
.
38
2.2.7.6.
QUANTITATIVE
PCR
OF
BMMF2
DNA
.
39
2.2.8.
SDS-PAGE
AND
WESTERN
BLOT
.
39
6
2.2.9.
IMMUNODETECTION
.
40
2.2.10.
PURIFICATION
OF
C2MI.1A.2
AND
C2MI.15B.6
REP
PROTEIN
.
41
2.2.11.
PURIFICATION
OF
C2MI.8B.7
REP
PROTEIN
.
2.2.12.
BRADFORD
ASSAY
.
2.2.13.
PRODUCTION
OF
MOUSE
MONOCLONAL
ANTIBODIES
AGAINST
BMMF2
REP
.
42
2.2.14.
ANTIBODY
VALIDATION
BY
ELISA
(CORE
FACILITY
DKF2)
.
42
2.2.15.
ANTIBODY
VALIDATION
BY
WB
AND
IF
MICROSCOPY
.
43
2.2.16.
EPITOPE
MAPPING
(WITH
PEPPERPRINT)
.
43
2.2.17.
IMMUNOPRECIPITATION
.
45
2.2.18.
IMMUNOHISTOCHEMISTRY
.
45
2.2.19.
PREPARATION
OF
TISSUE
AND
CELL
LYSATES
FOR
WB
AND
MASS
SPECTROMETRY
.
46
2.2.20.
MASS
SPECTROMETRY
ANALYSIS
(PROTEOMICS
CORE
FACILITY)
.
47
2.2.21.
LASER
MICRODISSECTION
(LMD)
.
48
2.2.22.
ROLLING-CIRCLE
AMPLIFICATION
(RCA)
.
48
2.2.23.
DIRECT
PCR
AMPLIFICATION
OF
BMMF2
.
48
2.2.24.
IN
SILICO
ANALYSIS
OF
THE
PRESENCE
OF
BMMF
SEQUENCES
IN
CANCER
PATIENTS
.
48
3.
RESULTS
.
50
3.1.
PHYLOGENY
OF
BMMF
AND
IDENTIFYING
REPRESENTATIVE
BMMF2
ISOLATES
SUITABLE
FOR
THE
PRODUCTION
OF
BMMF2
REP-SPECIFIC
ANTIBODIES
.
50
3.2.
BMMF2
IN
VITRO
CHARACTERISTICS
AFTER
TRANSFECTION
.
56
3.2.1.
BMMF2
REPLICATION
IN
CELL
CULTURE
.
56
3.2.2.
CHARACTERIZATION
OF
BMMF2
REP
EXPRESSION
IN
HUMAN
CELL
CULTURE
.
59
3.2.3.
PHENOTYPIC
ANALYSIS
OF
BMMF2
REP
OVEREXPRESSION
IN
HEK293TT
.
61
3.3.
PRODUCTION
AND
SELECTION
OF
BMMF2
REP-SPECIFIC
ANTIBODIES
.
62
3.3.1.
BMMF2
REP
PROTEIN
OVEREXPRESSION
AND
PURIFICATION
.
63
3.3.2.
IMMUNIZATION
STRATEGY
.
64
3.3.3.
SETUP
FOR
ANTIBODY
SCREENING AND
SELECTION
CRITERIA
OF
BMMF2
REP
ANTIBODY
CANDIDATES
65
3.4.
CHARACTERIZATION
OF
BMMF2
REP
ANTIBODIES
.
66
3.4.1.
IDENTIFICATION
OF
ANTIBODY
ISOTYPES
AND
LINEAR
EPITOPES
.
66
3.4.2.
SPECIFICITY
IN
WESTERN
BLOT
.
68
3.4.3.
SPECIFICITY
IN
IMMUNOFLUORESCENCE
MICROSCOPY
.
69
3.4.4.
IMMUNOPRECIPITATION
CAPABILITY
OF
BMMF2
REP
ANTIBODIES
.
69
3.4.5.
IHC
SPECIFICITY
BASED
ON
ANTIBODY
AND
ANTIGEN-DOSE
DEPENDENCY
.
70
3.4.6.
SUMMARY
OF
SELECTED
BMMF2
REP
ANTIBODIES
.
74
3.5.
BMMF2
IN
HUMAN
CANCER
.
75
3.5.1.
BMMF2
REP
EXPRESSION
IN
TISSUES
OF
CANCER
PATIENTS
.
75
7
3.5.2.
AFFIRMATION
OF
IHC
DETECTION
BY
BMMF2
TARGET
COMPETITION
.
82
3.5.3.
WHOLE
TISSUE
LYSIS
AND
SUBSEQUENT
MASS
SPECTROMETRY
ANALYSIS
.
84
3.5.4.
LASER
MICRODISSECTION
OF
PATIENT
TISSUE
FOR
BMMF2-SPECIFIC
PCR
.
85
3.5.5.
OVERVIEW
OF
HUMAN
MATERIAL
EXAMINED
FOR
BMMF2
.
85
3.5.6.
INTRINSIC
BMMF2
REP
PROTEIN
POSITIVITY
OF
CANCER CELL
LINES
.
86
3.5.7.
MASS
SPECTROMETRY
ANALYSIS
OF
BMMF-LIKE
PEPTIDES
FROM
CELL
LINES
.
87
4.
DISCUSSION.
89
4.1.
BMMF
PHYLOGENY
AND
SELECTION
OF
BMMF2
REP
IMMUNOGENS
FOR
PRODUCTION
OF
BMMF2
REP
SPECIFIC
ANTIBODIES
.
89
4.2.
IN
VITRO
BIOACTIVITY
OF
BMMF2
.
92
4.2.1.
BMMF2
REPLICATION
.
92
4.2.2.
BMMF2
REP
OVEREXPRESSION
AND
EFFECT
ON
VIABILITY
.
96
4.3.
BMMF2
REP
PROTEIN
PRODUCTION
AND
SELECTION
OF
BMMF2
REP-SPECIFIC
ANTIBODIES
.
98
4.4.
CHARACTERIZATION
OF
BMMF2
REP
ANTIBODIES
.
100
4.5.
BMMF2
IN
VIVO
.
104
4.5.1.
BMMF2
REP
IN
CANCER
PATIENT
TISSUES
(IHC)
.
104
4.5.2.
WHOLE
TISSUE
LYSIS
AND
SUBSEQUENT
MASS
SPECTROMETRY
ANALYSIS
.
108
4.5.2.
BMMF2
DNA
IN
CANCER
PATIENT
TISSUES
(LMD)
.
109
4.5.3.
ASSESSMENT
OF
BMMF2
ANTIBODY
APPLICABILITY
BASED
ON
IN
SILICO
ANALYSIS
OF
BMMF
IN
CANCER
DATABASES
.
ILL
4.5.3.
INTRINSIC
BMMF2
REP
PROTEIN
POSITIVITY
OF
CANCER CELL
LINES
.
112
5.
CONCLUSION
AND
OUTLOOK
.
115
6.
SUPPLEMENT
.
120
7.
LITERATURE
.
136
ABBREVIATIONS
.
144
ACKNOWLEDGMENTS
.
147 |
| any_adam_object | 1 |
| any_adam_object_boolean | 1 |
| author | Beck, Simon |
| author_GND | (DE-588)1058097989 (DE-588)121279731 |
| author_facet | Beck, Simon |
| author_role | aut |
| author_sort | Beck, Simon |
| author_variant | s b sb |
| building | Verbundindex |
| bvnumber | BV048961336 |
| ctrlnum | (OCoLC)1352255158 (DE-599)KXP1824053452 |
| discipline | Medizin |
| discipline_str_mv | Medizin |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>00000nam a2200000 c 4500</leader><controlfield tag="001">BV048961336</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="007">t|</controlfield><controlfield tag="008">230515s2022 gw a||| m||| 00||| eng d</controlfield><datafield tag="015" ind1=" " ind2=" "><subfield code="a">23,H01</subfield><subfield code="2">dnb</subfield></datafield><datafield tag="016" ind1="7" ind2=" "><subfield code="a">1274880688</subfield><subfield code="2">DE-101</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="c">Festeinband</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)1352255158</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)KXP1824053452</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="044" ind1=" " ind2=" "><subfield code="a">gw</subfield><subfield code="c">XA-DE</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="8">1\p</subfield><subfield code="a">616.994</subfield><subfield code="2">23ksdnb</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">610</subfield><subfield code="2">sdnb</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="8">2\p</subfield><subfield code="a">610</subfield><subfield code="2">23sdnb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Beck, Simon</subfield><subfield code="e">Verfasser</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Production and characterization of novel BMMF2 rep-specific antibodies and application in cancer histology</subfield><subfield code="c">presented by M. Sc. Simon Beck</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Heidelberg</subfield><subfield code="c">2022</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">148 Seiten</subfield><subfield code="b">Illustrationen, Diagramme</subfield><subfield code="c">31 cm</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="b">Dissertation</subfield><subfield code="c">Ruprechts-Karls-University Heidelberg</subfield><subfield code="d">2022</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Bartenschlager, Ralf</subfield><subfield code="d">1958-</subfield><subfield code="0">(DE-588)1058097989</subfield><subfield code="4">dgs</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zur Hausen, Harald</subfield><subfield code="d">1936-2023</subfield><subfield code="0">(DE-588)121279731</subfield><subfield code="4">dgs</subfield></datafield><datafield tag="710" ind1="2" ind2=" "><subfield code="a">Universität Heidelberg</subfield><subfield code="0">(DE-588)2024349-2</subfield><subfield code="4">dgg</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">B:DE-101</subfield><subfield code="q">application/pdf</subfield><subfield code="u">https://d-nb.info/1274880688/04</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=034225090&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="883" ind1="0" ind2=" "><subfield code="8">1\p</subfield><subfield code="a">emakn</subfield><subfield code="c">0,56841</subfield><subfield code="d">20221213</subfield><subfield code="q">DE-101</subfield><subfield code="u">https://d-nb.info/provenance/plan#emakn</subfield></datafield><datafield tag="883" ind1="2" ind2=" "><subfield code="8">2\p</subfield><subfield code="a">dnb</subfield><subfield code="d">20221214</subfield><subfield code="q">DE-101</subfield><subfield code="u">https://d-nb.info/provenance/plan#dnb</subfield></datafield><datafield tag="943" ind1="1" ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-034225090</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV048961336 |
| illustrated | Illustrated |
| index_date | 2024-07-03T22:00:28Z |
| indexdate | 2025-01-24T11:02:23Z |
| institution | BVB |
| institution_GND | (DE-588)2024349-2 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-034225090 |
| oclc_num | 1352255158 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | 148 Seiten Illustrationen, Diagramme 31 cm |
| publishDate | 2022 |
| publishDateSearch | 2022 |
| publishDateSort | 2022 |
| record_format | marc |
| spelling | Beck, Simon Verfasser aut Production and characterization of novel BMMF2 rep-specific antibodies and application in cancer histology presented by M. Sc. Simon Beck Heidelberg 2022 148 Seiten Illustrationen, Diagramme 31 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Ruprechts-Karls-University Heidelberg 2022 (DE-588)4113937-9 Hochschulschrift gnd-content Bartenschlager, Ralf 1958- (DE-588)1058097989 dgs Zur Hausen, Harald 1936-2023 (DE-588)121279731 dgs Universität Heidelberg (DE-588)2024349-2 dgg B:DE-101 application/pdf https://d-nb.info/1274880688/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=034225090&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p emakn 0,56841 20221213 DE-101 https://d-nb.info/provenance/plan#emakn 2\p dnb 20221214 DE-101 https://d-nb.info/provenance/plan#dnb |
| spellingShingle | Beck, Simon Production and characterization of novel BMMF2 rep-specific antibodies and application in cancer histology |
| subject_GND | (DE-588)4113937-9 |
| title | Production and characterization of novel BMMF2 rep-specific antibodies and application in cancer histology |
| title_auth | Production and characterization of novel BMMF2 rep-specific antibodies and application in cancer histology |
| title_exact_search | Production and characterization of novel BMMF2 rep-specific antibodies and application in cancer histology |
| title_exact_search_txtP | Production and characterization of novel BMMF2 rep-specific antibodies and application in cancer histology |
| title_full | Production and characterization of novel BMMF2 rep-specific antibodies and application in cancer histology presented by M. Sc. Simon Beck |
| title_fullStr | Production and characterization of novel BMMF2 rep-specific antibodies and application in cancer histology presented by M. Sc. Simon Beck |
| title_full_unstemmed | Production and characterization of novel BMMF2 rep-specific antibodies and application in cancer histology presented by M. Sc. Simon Beck |
| title_short | Production and characterization of novel BMMF2 rep-specific antibodies and application in cancer histology |
| title_sort | production and characterization of novel bmmf2 rep specific antibodies and application in cancer histology |
| topic_facet | Hochschulschrift |
| url | https://d-nb.info/1274880688/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=034225090&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT becksimon productionandcharacterizationofnovelbmmf2repspecificantibodiesandapplicationincancerhistology AT bartenschlagerralf productionandcharacterizationofnovelbmmf2repspecificantibodiesandapplicationincancerhistology AT zurhausenharald productionandcharacterizationofnovelbmmf2repspecificantibodiesandapplicationincancerhistology AT universitatheidelberg productionandcharacterizationofnovelbmmf2repspecificantibodiesandapplicationincancerhistology |
There is no print copy available.
Indexes