Identification of novel mechanisms regulating the hepatic BMP-hepcidin axis and iron homeostasis:
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| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Heidelberg
2022
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| Schlagworte: | |
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| Beschreibung: | X, 128 Seiten Illustrationen, Diagramme 30 cm |
Internformat
MARC
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| 016 | 7 | |a 1267932589 |2 DE-101 | |
| 020 | |c Broschur | ||
| 035 | |a (OCoLC)1345061555 | ||
| 035 | |a (DE-599)DNB1267932589 | ||
| 040 | |a DE-604 |b ger |e rda | ||
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| 100 | 1 | |a Colucci, Silvia |d 1992- |e Verfasser |0 (DE-588)132809605 |4 aut | |
| 245 | 1 | 0 | |a Identification of novel mechanisms regulating the hepatic BMP-hepcidin axis and iron homeostasis |c presented by Silvia Colucci, M.Sc. |
| 264 | 1 | |a Heidelberg |c 2022 | |
| 300 | |a X, 128 Seiten |b Illustrationen, Diagramme |c 30 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Ruprecht-Karls-University Heidelberg |d 2022 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 856 | 4 | 2 | |m B:DE-101 |q application/pdf |u https://d-nb.info/1267932589/04 |3 Inhaltsverzeichnis |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=034180605&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-034180605 | ||
| 883 | 0 | |8 1\p |a emakn |c 0,07920 |d 20220919 |q DE-101 |u https://d-nb.info/provenance/plan#emakn | |
| 883 | 2 | |8 2\p |a dnb |d 20220928 |q DE-101 |u https://d-nb.info/provenance/plan#dnb | |
| 883 | 2 | |8 3\p |a dnb |d 20220928 |q DE-101 |u https://d-nb.info/provenance/plan#dnb | |
Datensatz im Suchindex
| _version_ | 1804185092949540864 |
|---|---|
| adam_text | T
ABLE
OF
C
ONTENTS
D
ISCLOSURES
..........................................................................................
I
D
ECLARATION
OF
C
ONTRIBUTIONS
....................................................................................
II
L
IST
OF
A
BBREVIATIONS
..........................................................................................
ILL
L
IST
OF
T
ABLES
.....................................................................................................
VII
L
IST
OF
F
IGURES
....................................................................................................
VII
L
IST
OF
P
UBLICATIONS
............................................................................................
IX
F
IRST
A
UTHOR
P
UBLICATIONS
........................................................................................
IX
C
O
-
AUTHORSHIPS
......................................................................
IX
R
EVIEWS
.........................................................................................................................
X
1.
I
NTRODUCTION
...................................................................................................
1
1.1
T
HE
IRE-IRP
S
YSTEM
C
ONTROLS
C
ELLULAR
I
RON
H
OMEOSTASIS
...........................
2
1.2
T
HE
H
EPCIDIN
-FPN
A
XIS
C
ONTROLS
S
YSTEMIC
I
RON
H
OMEOSTASIS
.......................
4
1.2.1
THE
IRON
EXPORTER
FERROPORTIN;
A
HEPCIDIN
RECEPTOR
........................................................
4
1.2.2
HEPCIDIN:
THE
HEPATOCYTIC
HORMONE
CONTROLLING
FPN-MEDIATED
IRON
RELEASE
...........
5
1.3
M
OLECULAR
M
ECHANISMS
OF
H
EPCIDIN
R
EGULATION
.............................................
6
1.3.1
THE
SMAD1/5/8
SIGNALLING
PATHWAY
............................................................................
6
1.3.2
INTRACELLULAR
REGULATORS
OF
THE
BMP-SMAD
SIGNALLING
PATHWAY
..................................
8
1.3.3
CELL
SURFACE
REGULATORS
OF
THE
BMP-SMAD
SIGNALLING
PATHWAY
...............................
8
1.3.4
EXTRACELLULAR
REGULATORS
OF
THE
BMP-SMAD
SIGNALLING
PATHWAY
...............................
10
1.3.5
THE
JAK2-STAT3
SIGNALLING
PATHWAY
.......................................................................
11
1.4
I
RON
-
DEPENDENT
H
EPCIDIN
R
EGULATION
..............................................................
13
1.5
H
EPCIDIN
R
EGULATION
M
EDIATED
BY
E
RYTHROPOIESIS
........................................
15
1.6
H
EPCIDIN
R
EGULATION
IN
R
ESPONSE
TO
I
NFLAMMATION
.......................................
16
1.7
C
LINICAL
C
ONSEQUENCES
OF
H
EPCIDIN
-FPN
A
XIS
D
EREGULATION
AND
R
ELATED
M
OUSE
M
ODELS
............................................................................................................
17
1.7.1
GENETIC
AND
ACQUIRED
IRON
OVERLOAD
DISORDERS
.........................................................
17
1.7.2
GENETIC
AND
ACQUIRED
IRON
DEFICIENCY
DISORDERS
.......................................................
19
1.8
L
IVER
C
ELLS
AND
THEIR
R
OLE
IN
I
RON
M
ETABOLISM
..............................................
20
1.8.1
HEPATOCYTES
AND
CHOLANGIOCYTES
.............................................................................
23
1.8.2
LIVER
(SINUSOIDAL)
ENDOTHELIAL
CELLS
(LSECS)
...........................................................
24
1.8.3
HEPATIC
STELLATE
CELLS
(HSCS)
..................................................................................
27
1.8.4
KUPFFER
CELLS
(KCS)
....................................................................................................
27
1.9
A
IM
OF
THE
S
TUDY
...............................................................................................
28
2.
M
ATERIAL
AND
M
ETHODS
.................................................................................
29
2.1
K
EY
R
EAGENTS
AND
R
ESOURCES
.........................................................................
29
2.2
B
UFFERS
...............................................................................................................
34
2.3
A
NIMAL
M
ODELS
....................................................................................................
35
2.3.
1
DIETARY
MOUSE
MODEL
OF
IRON
OVERLOAD
......................................................................35
2.3.2 GENETIC
MOUSE
MODELS
OF
IRON
OVERLOAD
...................................................................35
2.3.3 MOUSE
TREATMENT
WITH
TGF^,
LY2157299
AND
BMP2
...............................................36
2.3.4
WILD-TYPE
MICE
AND
RATS
FOR
PRIMARY
CELL
CULTURE
PREPARATION
.................................36
2.4
L
IVER
P
ERFUSION
AND
I
SOLATION
OF
HCS,
HSCS,
KCS,
LSECS
..........................
37
2.5
C
ELL
C
ULTURE
C
ONDITIONS
..................................................................................
38
2.5.1
HEPA1-6
(MOUSE
HEPATOCYTE
CELL
LINE)
.....................................................................38
2.5.2
PRIMARY
MOUSE
AND
RAT
LSECS
..................................................................................38
2.
5.
3
PRIMARY
MOUSE
AND
RAT
HEPATOCYTES
........................................................................
38
2.5.4
LIVER
CELL
CULTURE
......................................................................................................
38
2.5.5 BONE
MARROW
DERIVED
MACROPHAGES
(BMDMS)
.........................................................39
2.6
C
ELL
C
ULTURE
T
REATMENTS
..............................................................................
39
2.6.1
TREATMENTS
OF
PRIMARY
CELL
CULTURE
PREPARATIONS
......................................................
39
2.
6.
2
TREATMENTS
OF
PRIMARY
LSECS
WITH
HEPATOCYTE
CONDITIONED
MEDIUM.......................40
2.7
SIRNA
M
EDIATED
K
NOCK
-
DOWN
IN
P
RIMARY
M
OUSE
LSECS
................................
40
2.8
S
CANNING
E
LECTRON
M
ICROSCOPY
(SEM)
..........................................................
41
2.9
P
HASE
C
ONTRAST
I
MAGING
OF
LSECS
.................................................................
41
2.10
F
LUORESCENCE
A
CTIVATED
C
ELL
S
ORTING
(FACS)
A
NALYSIS
............................
41
2.11
H
AEMATOLOGICAL
P
ARAMETERS
AND
S
ERUM
I
RON
M
EASUREMENT
.....................
41
2.12
T
ISSUE
I
RON
M
EASUREMENT
..............................................................................
42
2.13
SDS-PAGE
AND
WESTERN
BLOT
.......................................................................................
42
2.14
RNA
E
XTRACTION
,
QC,
R
EVERSE
T
RANSCRIPTION
,
AND
Q
RT-PCR
...................
42
2.15
RNA
S
EQUENCING
AND
L
IGAND
-R
ECEPTOR
I
NTERACTION
A
NALYSIS
..................
43
2.16
H
EPATOCYTE
M
EDIUM
F
RACTIONATION
AND
M
ASS
S
PECTROMETRY
....................
43
2.17
S
TATISTICAL
A
NALYSIS
.......................................................................................
44
3.
R
ESULTS
.........................................................................................................45
3.1
ISOLATION
OF
MURINE
LIVER
CELLS
......................................................................................
45
3.1.1
SUMMARY
1
...................................................................................................................
47
3.2
IRON-DEPENDENT
BMP6
INDUCTION
IN
LSECS
IS
CONTROLLED
BY
HEPATOCYTE
S
ECRETORY
S
IGNALS
...................................................................................................
51
3.2.1
IRON
ACCUMULATION
IN
LSECS
IS
NOT
A
PREREQUISITE
TO
INDUCE
BMP6
EXPRESSION
IN
VIVO
AND
EX
VIVO
.............................................................................................................................
54
3.2.2
THE
HEPATOCYTE
SECRETOME
CONTROLS
IRON-MEDIATED
BMP6
INDUCTION
IN
LSECS......
60
3.2.
3
IDENTIFICATION
OF
HEPATOCYTE-DERIVED
MOLECULES
REQUIRED
FOR
THE
IRON-MEDIATED
BMP6
INDUCTION
IN
LSECS
.................................................................................................................
63
3.2.4
BMP6
MRNA
UPREGULATION
IN
LSECS
REQUIRES
DE
NOVO
TRANSCRIPTION
ACTIVATED
BY
THE
MAPK-ERK
SIGNALLING
PATHWAY
.......................................................................................
73
3.2.5 SUMMARY
II
................................................................................................................
76
3.3
TGF
B
P
ATHWAY
A
CTIVATION
IN
LSECS
S
UPPRESSES
BMP2
E
XPRESSION
...........
77
3.3.1
LSECS
DO
NOT
RESPOND
TO
BMP2
AND
BMP6
EX
VIVO
.................................................
80
3.3.2
LSEC
DO
NOT
RESPOND
TO
HIGHER
BMP6 LEVELS
IN
MOUSE
MODELS
OF
IRON
OVERLOAD..
83
3.3.3
TGF^1
REDUCES
BMP2 EXPRESSION
VIA
ALK5
ACTIVATION
............................................
86
3.3.4
SUMMARY
III
................................................................................................................
91
3.4
S
ENSING
OF
LSEC
RELEASED
BMPS
BY
H
EPATOCYTES
DOES
NOT
R
EQUIRE
THE
E
XPRESSION
OF
HFE,
HJV
AND
TFR2
...........................................................................
92
3.4.1
HEMOCHROMATOSIS
PROTEINS
ARE
DISPENSABLE
FOR
THE
ACUTE
HEPCIDIN
RESPONSE
TO
BMP2
EX
VIVO
AND
IN
VIVO........................................................................................................
92
3.4.2
ANALYSIS
OF
BMP
CANONICAL
AND
NON-CANONICAL
PATHWAYS
IN
WILD-TYPE,
HJV
KO
AND
TFR2
KO
MICE
TREATED
WITH
BMP2
...........................................................................................
96
3.4.3 BMP2
INDUCES
A
PRO-INFLAMMATORY
RESPONSE
IN
VIVO
...............................................98
3.4.4
SUMMARY
IV.
.............................................................................................................
702
4.
D
ISCUSSION
....................................................................................................
103
4.1
P
RO
AND
C
ONS
OF
LSEC
P
RIMARY
C
ULTURE
P
REPARATION
..............................
103
4.2
A
N
OVEL
W
ORKING
M
ODEL
FOR
THE
I
RON
-
MEDIATED
BMP6
R
EGULATION
..........
104
4.3
R
OLE
OF
D
IFFERENT
L
IVER
C
ELL
T
YPES
IN
BMP
P
RODUCTION
............................
106
4.4
A
NALYSIS
OF
LSEC
RESPONSE
TO
TGF
B
/BMP
L
IGANDS
....................................
107
4.5
TGFB-ALK5
ACTIVITY
CONTROL
BMP2
EXPRESSION
IN
LSECS:
T
RANSLATIONAL
P
ERSPECTIVES
............................................................................................................
108
4.6
S
UMMARY
..........................................................................................................
109
5.
C
ONCLUSIONS
AND
F
UTURE
P
ERSPECTIVES
....................................................
110
6.
R
EFERENCES
.................................................................................................
112
A
CKNOWLEDGMENTS
............................................................................................
127
|
| adam_txt |
T
ABLE
OF
C
ONTENTS
D
ISCLOSURES
.
I
D
ECLARATION
OF
C
ONTRIBUTIONS
.
II
L
IST
OF
A
BBREVIATIONS
.
ILL
L
IST
OF
T
ABLES
.
VII
L
IST
OF
F
IGURES
.
VII
L
IST
OF
P
UBLICATIONS
.
IX
F
IRST
A
UTHOR
P
UBLICATIONS
.
IX
C
O
-
AUTHORSHIPS
.
IX
R
EVIEWS
.
X
1.
I
NTRODUCTION
.
1
1.1
T
HE
IRE-IRP
S
YSTEM
C
ONTROLS
C
ELLULAR
I
RON
H
OMEOSTASIS
.
2
1.2
T
HE
H
EPCIDIN
-FPN
A
XIS
C
ONTROLS
S
YSTEMIC
I
RON
H
OMEOSTASIS
.
4
1.2.1
THE
IRON
EXPORTER
FERROPORTIN;
A
HEPCIDIN
RECEPTOR
.
4
1.2.2
HEPCIDIN:
THE
HEPATOCYTIC
HORMONE
CONTROLLING
FPN-MEDIATED
IRON
RELEASE
.
5
1.3
M
OLECULAR
M
ECHANISMS
OF
H
EPCIDIN
R
EGULATION
.
6
1.3.1
THE
SMAD1/5/8
SIGNALLING
PATHWAY
.
6
1.3.2
INTRACELLULAR
REGULATORS
OF
THE
BMP-SMAD
SIGNALLING
PATHWAY
.
8
1.3.3
CELL
SURFACE
REGULATORS
OF
THE
BMP-SMAD
SIGNALLING
PATHWAY
.
8
1.3.4
EXTRACELLULAR
REGULATORS
OF
THE
BMP-SMAD
SIGNALLING
PATHWAY
.
10
1.3.5
THE
JAK2-STAT3
SIGNALLING
PATHWAY
.
11
1.4
I
RON
-
DEPENDENT
H
EPCIDIN
R
EGULATION
.
13
1.5
H
EPCIDIN
R
EGULATION
M
EDIATED
BY
E
RYTHROPOIESIS
.
15
1.6
H
EPCIDIN
R
EGULATION
IN
R
ESPONSE
TO
I
NFLAMMATION
.
16
1.7
C
LINICAL
C
ONSEQUENCES
OF
H
EPCIDIN
-FPN
A
XIS
D
EREGULATION
AND
R
ELATED
M
OUSE
M
ODELS
.
17
1.7.1
GENETIC
AND
ACQUIRED
IRON
OVERLOAD
DISORDERS
.
17
1.7.2
GENETIC
AND
ACQUIRED
IRON
DEFICIENCY
DISORDERS
.
19
1.8
L
IVER
C
ELLS
AND
THEIR
R
OLE
IN
I
RON
M
ETABOLISM
.
20
1.8.1
HEPATOCYTES
AND
CHOLANGIOCYTES
.
23
1.8.2
LIVER
(SINUSOIDAL)
ENDOTHELIAL
CELLS
(LSECS)
.
24
1.8.3
HEPATIC
STELLATE
CELLS
(HSCS)
.
27
1.8.4
KUPFFER
CELLS
(KCS)
.
27
1.9
A
IM
OF
THE
S
TUDY
.
28
2.
M
ATERIAL
AND
M
ETHODS
.
29
2.1
K
EY
R
EAGENTS
AND
R
ESOURCES
.
29
2.2
B
UFFERS
.
34
2.3
A
NIMAL
M
ODELS
.
35
2.3.
1
DIETARY
MOUSE
MODEL
OF
IRON
OVERLOAD
.35
2.3.2 GENETIC
MOUSE
MODELS
OF
IRON
OVERLOAD
.35
2.3.3 MOUSE
TREATMENT
WITH
TGF^,
LY2157299
AND
BMP2
.36
2.3.4
WILD-TYPE
MICE
AND
RATS
FOR
PRIMARY
CELL
CULTURE
PREPARATION
.36
2.4
L
IVER
P
ERFUSION
AND
I
SOLATION
OF
HCS,
HSCS,
KCS,
LSECS
.
37
2.5
C
ELL
C
ULTURE
C
ONDITIONS
.
38
2.5.1
HEPA1-6
(MOUSE
HEPATOCYTE
CELL
LINE)
.38
2.5.2
PRIMARY
MOUSE
AND
RAT
LSECS
.38
2.
5.
3
PRIMARY
MOUSE
AND
RAT
HEPATOCYTES
.
38
2.5.4
LIVER
CELL
CULTURE
.
38
2.5.5 BONE
MARROW
DERIVED
MACROPHAGES
(BMDMS)
.39
2.6
C
ELL
C
ULTURE
T
REATMENTS
.
39
2.6.1
TREATMENTS
OF
PRIMARY
CELL
CULTURE
PREPARATIONS
.
39
2.
6.
2
TREATMENTS
OF
PRIMARY
LSECS
WITH
HEPATOCYTE
CONDITIONED
MEDIUM.40
2.7
SIRNA
M
EDIATED
K
NOCK
-
DOWN
IN
P
RIMARY
M
OUSE
LSECS
.
40
2.8
S
CANNING
E
LECTRON
M
ICROSCOPY
(SEM)
.
41
2.9
P
HASE
C
ONTRAST
I
MAGING
OF
LSECS
.
41
2.10
F
LUORESCENCE
A
CTIVATED
C
ELL
S
ORTING
(FACS)
A
NALYSIS
.
41
2.11
H
AEMATOLOGICAL
P
ARAMETERS
AND
S
ERUM
I
RON
M
EASUREMENT
.
41
2.12
T
ISSUE
I
RON
M
EASUREMENT
.
42
2.13
SDS-PAGE
AND
WESTERN
BLOT
.
42
2.14
RNA
E
XTRACTION
,
QC,
R
EVERSE
T
RANSCRIPTION
,
AND
Q
RT-PCR
.
42
2.15
RNA
S
EQUENCING
AND
L
IGAND
-R
ECEPTOR
I
NTERACTION
A
NALYSIS
.
43
2.16
H
EPATOCYTE
M
EDIUM
F
RACTIONATION
AND
M
ASS
S
PECTROMETRY
.
43
2.17
S
TATISTICAL
A
NALYSIS
.
44
3.
R
ESULTS
.45
3.1
ISOLATION
OF
MURINE
LIVER
CELLS
.
45
3.1.1
SUMMARY
1
.
47
3.2
IRON-DEPENDENT
BMP6
INDUCTION
IN
LSECS
IS
CONTROLLED
BY
HEPATOCYTE
S
ECRETORY
S
IGNALS
.
51
3.2.1
IRON
ACCUMULATION
IN
LSECS
IS
NOT
A
PREREQUISITE
TO
INDUCE
BMP6
EXPRESSION
IN
VIVO
AND
EX
VIVO
.
54
3.2.2
THE
HEPATOCYTE
SECRETOME
CONTROLS
IRON-MEDIATED
BMP6
INDUCTION
IN
LSECS.
60
3.2.
3
IDENTIFICATION
OF
HEPATOCYTE-DERIVED
MOLECULES
REQUIRED
FOR
THE
IRON-MEDIATED
BMP6
INDUCTION
IN
LSECS
.
63
3.2.4
BMP6
MRNA
UPREGULATION
IN
LSECS
REQUIRES
DE
NOVO
TRANSCRIPTION
ACTIVATED
BY
THE
MAPK-ERK
SIGNALLING
PATHWAY
.
73
3.2.5 SUMMARY
II
.
76
3.3
TGF
B
P
ATHWAY
A
CTIVATION
IN
LSECS
S
UPPRESSES
BMP2
E
XPRESSION
.
77
3.3.1
LSECS
DO
NOT
RESPOND
TO
BMP2
AND
BMP6
EX
VIVO
.
80
3.3.2
LSEC
DO
NOT
RESPOND
TO
HIGHER
BMP6 LEVELS
IN
MOUSE
MODELS
OF
IRON
OVERLOAD.
83
3.3.3
TGF^1
REDUCES
BMP2 EXPRESSION
VIA
ALK5
ACTIVATION
.
86
3.3.4
SUMMARY
III
.
91
3.4
S
ENSING
OF
LSEC
RELEASED
BMPS
BY
H
EPATOCYTES
DOES
NOT
R
EQUIRE
THE
E
XPRESSION
OF
HFE,
HJV
AND
TFR2
.
92
3.4.1
HEMOCHROMATOSIS
PROTEINS
ARE
DISPENSABLE
FOR
THE
ACUTE
HEPCIDIN
RESPONSE
TO
BMP2
EX
VIVO
AND
IN
VIVO.
92
3.4.2
ANALYSIS
OF
BMP
CANONICAL
AND
NON-CANONICAL
PATHWAYS
IN
WILD-TYPE,
HJV
KO
AND
TFR2
KO
MICE
TREATED
WITH
BMP2
.
96
3.4.3 BMP2
INDUCES
A
PRO-INFLAMMATORY
RESPONSE
IN
VIVO
.98
3.4.4
SUMMARY
IV.
.
702
4.
D
ISCUSSION
.
103
4.1
P
RO
AND
C
ONS
OF
LSEC
P
RIMARY
C
ULTURE
P
REPARATION
.
103
4.2
A
N
OVEL
W
ORKING
M
ODEL
FOR
THE
I
RON
-
MEDIATED
BMP6
R
EGULATION
.
104
4.3
R
OLE
OF
D
IFFERENT
L
IVER
C
ELL
T
YPES
IN
BMP
P
RODUCTION
.
106
4.4
A
NALYSIS
OF
LSEC
RESPONSE
TO
TGF
B
/BMP
L
IGANDS
.
107
4.5
TGFB-ALK5
ACTIVITY
CONTROL
BMP2
EXPRESSION
IN
LSECS:
T
RANSLATIONAL
P
ERSPECTIVES
.
108
4.6
S
UMMARY
.
109
5.
C
ONCLUSIONS
AND
F
UTURE
P
ERSPECTIVES
.
110
6.
R
EFERENCES
.
112
A
CKNOWLEDGMENTS
.
127 |
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| author | Colucci, Silvia 1992- |
| author_GND | (DE-588)132809605 |
| author_facet | Colucci, Silvia 1992- |
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| bvnumber | BV048916487 |
| ctrlnum | (OCoLC)1345061555 (DE-599)DNB1267932589 |
| discipline | Medizin |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV048916487 |
| illustrated | Illustrated |
| index_date | 2024-07-03T21:54:47Z |
| indexdate | 2024-07-10T09:49:45Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-034180605 |
| oclc_num | 1345061555 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | X, 128 Seiten Illustrationen, Diagramme 30 cm |
| publishDate | 2022 |
| publishDateSearch | 2022 |
| publishDateSort | 2022 |
| record_format | marc |
| spelling | Colucci, Silvia 1992- Verfasser (DE-588)132809605 aut Identification of novel mechanisms regulating the hepatic BMP-hepcidin axis and iron homeostasis presented by Silvia Colucci, M.Sc. Heidelberg 2022 X, 128 Seiten Illustrationen, Diagramme 30 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Ruprecht-Karls-University Heidelberg 2022 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf https://d-nb.info/1267932589/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=034180605&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p emakn 0,07920 20220919 DE-101 https://d-nb.info/provenance/plan#emakn 2\p dnb 20220928 DE-101 https://d-nb.info/provenance/plan#dnb 3\p dnb 20220928 DE-101 https://d-nb.info/provenance/plan#dnb |
| spellingShingle | Colucci, Silvia 1992- Identification of novel mechanisms regulating the hepatic BMP-hepcidin axis and iron homeostasis |
| subject_GND | (DE-588)4113937-9 |
| title | Identification of novel mechanisms regulating the hepatic BMP-hepcidin axis and iron homeostasis |
| title_auth | Identification of novel mechanisms regulating the hepatic BMP-hepcidin axis and iron homeostasis |
| title_exact_search | Identification of novel mechanisms regulating the hepatic BMP-hepcidin axis and iron homeostasis |
| title_exact_search_txtP | Identification of novel mechanisms regulating the hepatic BMP-hepcidin axis and iron homeostasis |
| title_full | Identification of novel mechanisms regulating the hepatic BMP-hepcidin axis and iron homeostasis presented by Silvia Colucci, M.Sc. |
| title_fullStr | Identification of novel mechanisms regulating the hepatic BMP-hepcidin axis and iron homeostasis presented by Silvia Colucci, M.Sc. |
| title_full_unstemmed | Identification of novel mechanisms regulating the hepatic BMP-hepcidin axis and iron homeostasis presented by Silvia Colucci, M.Sc. |
| title_short | Identification of novel mechanisms regulating the hepatic BMP-hepcidin axis and iron homeostasis |
| title_sort | identification of novel mechanisms regulating the hepatic bmp hepcidin axis and iron homeostasis |
| topic_facet | Hochschulschrift |
| url | https://d-nb.info/1267932589/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=034180605&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT coluccisilvia identificationofnovelmechanismsregulatingthehepaticbmphepcidinaxisandironhomeostasis |
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