Synaptic vesicles: methods and protocols
This book provides protocols on the preparation of synaptic vesicles from different model systems and provides instructions for functional investigations of their transport and formation, scaffolding, filling and priming, release, recycling, and degradation. Chapters guide readers through computatio...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Humana Press
[2022]
|
| Schriftenreihe: | Methods in molecular biology
2417 |
| Schlagworte: | |
| Zusammenfassung: | This book provides protocols on the preparation of synaptic vesicles from different model systems and provides instructions for functional investigations of their transport and formation, scaffolding, filling and priming, release, recycling, and degradation. Chapters guide readers through computational approaches, simulating different aspects of synaptic vesicle anatomy and function. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Synaptic Vesicles: Methods and Protocols aims to be a useful practical guide to researchers to help further their study in this field |
| Beschreibung: | 1. 1. Real-time quantitative PCR and fluorescence in situ hybridization for subcellular localization of miRNAs in neurons.; Antonis Tatarakis and Danesh Moazed; 2. Live-imaging of axonal cargoes in Drosophila brain explants using confocal microscopy.; Caroline Medioni, Anne Ephrussi and Florence Besse; 3. Whole Endosome Recording of Vesicular Neurotransmitter Transporter Currents.; Roger Chang and Robert H. Edwards; 4. Quantitative analysis of presynaptic vesicle luminal pH in cultured neurons.; Yoshihiro Egashira, Shutaro Katsurabayashi and Shigeo Takamori; 5. Investigation of synaptic vesicle proteins in rat brain tissue using real-time qPCR.; Betina Elfving; 6. Mass Synaptometry: Applying Mass Cytometry to Single Synapse Analysis.; Chandresh R. Gajera, Rosemary Fernandez, Nadia Postupna, Kathleen S. Montine, C. Dirk Keene, Sean C. Bendall, Thomas J. Montine; 7. - A Guide to Analysis of Relative Synaptic Protein Abundance by Quantitative Fluorescent Western Blotting.; Heidi Kaastrup Müller; 8. Synaptosomes and metamodulation of receptors.; Anna Pittaluga and Mario Marchi; 9. A novel method to monitor GABA loading into synaptic vesicles by combining patch pipette perfusion and intracellular, caged-GABA photolysis in brain slice preparations.; Manami Yamashita and Tetsuya Hori; 10. Rapid Isolation of Functional Synaptic Vesicles from Tissues through Cryo-grinding, Ultracentrifugation and Size Exclusion Chromatography.; Huinan Marcus Li; 11. Isolation of synaptic vesicles from mammalian brain; Marcelo Ganzella, Momchil Ninov, Dietmar Riedel, Reinhard Jahn; 12. Building and Using a Two-Photon Fluorescence Crosscorrelation Spectroscopy Setup including Fluorescence Lifetime Analysis.; Tobias Grothe and Peter J. Walla; 13. - Fluorescence Lifetime and Cross-correlation Spectroscopy for Observing Membrane Fusion of Liposome Models Containing Synaptic Proteins.; Tobias Grothe and Peter J. Walla; 14. Synaptic vesicle pool monitoring with synaptopH-Iuorin. ; Marc Dahlmanns and Jana Katharina Dahlmanns; 15. Imaging neuropeptide release at Drosophila neuromuscular junction with a genetically engineered neuropeptide release reporter.; Yifu Han and Keke Ding; 16. Imaging synaptic glutamate release with two-photon microscopy in organotypic slice cultures; Céline D. Dürst and Thomas G. Oertner; 17. Dynole 34-2 and Acrylo-Dyn 2-30, novel dynamin GTPase chemical biology probes.; Jennifer R. Baker, Nicholas S. O’Brien, Kate L. Prichard, Phillip J. Robinson, Adam McCluskey and Cecilia C. Russell; 18. Synthesis of Pthaladyn-29 and Napthalimide-10, GTP site directed dynamin GTPase inhibitors. ; Cecilia C. Russell, Kate L. Prichard, Nicholas S. O’Brien, Adam McCluskey, Phillip J. Robinson and Jennifer R. Baker |
| Beschreibung: | xi, 260 Seiten Illustrationen, Diagramme 522 grams |
| ISBN: | 9781071619186 |
Internformat
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| 500 | |a - A Guide to Analysis of Relative Synaptic Protein Abundance by Quantitative Fluorescent Western Blotting.; Heidi Kaastrup Müller; 8. Synaptosomes and metamodulation of receptors.; Anna Pittaluga and Mario Marchi; 9. A novel method to monitor GABA loading into synaptic vesicles by combining patch pipette perfusion and intracellular, caged-GABA photolysis in brain slice preparations.; Manami Yamashita and Tetsuya Hori; 10. Rapid Isolation of Functional Synaptic Vesicles from Tissues through Cryo-grinding, Ultracentrifugation and Size Exclusion Chromatography.; Huinan Marcus Li; 11. Isolation of synaptic vesicles from mammalian brain; Marcelo Ganzella, Momchil Ninov, Dietmar Riedel, Reinhard Jahn; 12. Building and Using a Two-Photon Fluorescence Crosscorrelation Spectroscopy Setup including Fluorescence Lifetime Analysis.; Tobias Grothe and Peter J. Walla; 13. | ||
| 500 | |a - Fluorescence Lifetime and Cross-correlation Spectroscopy for Observing Membrane Fusion of Liposome Models Containing Synaptic Proteins.; Tobias Grothe and Peter J. Walla; 14. Synaptic vesicle pool monitoring with synaptopH-Iuorin. ; Marc Dahlmanns and Jana Katharina Dahlmanns; 15. Imaging neuropeptide release at Drosophila neuromuscular junction with a genetically engineered neuropeptide release reporter.; Yifu Han and Keke Ding; 16. Imaging synaptic glutamate release with two-photon microscopy in organotypic slice cultures; Céline D. Dürst and Thomas G. Oertner; 17. Dynole 34-2 and Acrylo-Dyn 2-30, novel dynamin GTPase chemical biology probes.; Jennifer R. Baker, Nicholas S. O’Brien, Kate L. Prichard, Phillip J. Robinson, Adam McCluskey and Cecilia C. Russell; 18. Synthesis of Pthaladyn-29 and Napthalimide-10, GTP site directed dynamin GTPase inhibitors. ; Cecilia C. Russell, Kate L. Prichard, Nicholas S. O’Brien, Adam McCluskey, Phillip J. Robinson and Jennifer R. Baker | ||
| 520 | |a This book provides protocols on the preparation of synaptic vesicles from different model systems and provides instructions for functional investigations of their transport and formation, scaffolding, filling and priming, release, recycling, and degradation. Chapters guide readers through computational approaches, simulating different aspects of synaptic vesicle anatomy and function. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Synaptic Vesicles: Methods and Protocols aims to be a useful practical guide to researchers to help further their study in this field | ||
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Datensatz im Suchindex
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| id | DE-604.BV048907653 |
| illustrated | Illustrated |
| index_date | 2024-07-03T21:52:34Z |
| indexdate | 2024-07-31T01:19:00Z |
| institution | BVB |
| isbn | 9781071619186 |
| language | English |
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| physical | xi, 260 Seiten Illustrationen, Diagramme 522 grams |
| publishDate | 2022 |
| publishDateSearch | 2022 |
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| publisher | Humana Press |
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| series | Methods in molecular biology |
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| spelling | Synaptic vesicles methods and protocols edited by Jana Dahlmanns (Department of Psychiatry and Psychotherapy, Universitätsklinikum Erlangen, Erlangen, Bayern Germany), Marc Dahlmanns (Institute of Physiology and Pathophysiology, University of Erlangen-Nuremberg, Erlangen, Germany) New York, NY Humana Press [2022] xi, 260 Seiten Illustrationen, Diagramme 522 grams txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 2417 1. 1. Real-time quantitative PCR and fluorescence in situ hybridization for subcellular localization of miRNAs in neurons.; Antonis Tatarakis and Danesh Moazed; 2. Live-imaging of axonal cargoes in Drosophila brain explants using confocal microscopy.; Caroline Medioni, Anne Ephrussi and Florence Besse; 3. Whole Endosome Recording of Vesicular Neurotransmitter Transporter Currents.; Roger Chang and Robert H. Edwards; 4. Quantitative analysis of presynaptic vesicle luminal pH in cultured neurons.; Yoshihiro Egashira, Shutaro Katsurabayashi and Shigeo Takamori; 5. Investigation of synaptic vesicle proteins in rat brain tissue using real-time qPCR.; Betina Elfving; 6. Mass Synaptometry: Applying Mass Cytometry to Single Synapse Analysis.; Chandresh R. Gajera, Rosemary Fernandez, Nadia Postupna, Kathleen S. Montine, C. Dirk Keene, Sean C. Bendall, Thomas J. Montine; 7. - A Guide to Analysis of Relative Synaptic Protein Abundance by Quantitative Fluorescent Western Blotting.; Heidi Kaastrup Müller; 8. Synaptosomes and metamodulation of receptors.; Anna Pittaluga and Mario Marchi; 9. A novel method to monitor GABA loading into synaptic vesicles by combining patch pipette perfusion and intracellular, caged-GABA photolysis in brain slice preparations.; Manami Yamashita and Tetsuya Hori; 10. Rapid Isolation of Functional Synaptic Vesicles from Tissues through Cryo-grinding, Ultracentrifugation and Size Exclusion Chromatography.; Huinan Marcus Li; 11. Isolation of synaptic vesicles from mammalian brain; Marcelo Ganzella, Momchil Ninov, Dietmar Riedel, Reinhard Jahn; 12. Building and Using a Two-Photon Fluorescence Crosscorrelation Spectroscopy Setup including Fluorescence Lifetime Analysis.; Tobias Grothe and Peter J. Walla; 13. - Fluorescence Lifetime and Cross-correlation Spectroscopy for Observing Membrane Fusion of Liposome Models Containing Synaptic Proteins.; Tobias Grothe and Peter J. Walla; 14. Synaptic vesicle pool monitoring with synaptopH-Iuorin. ; Marc Dahlmanns and Jana Katharina Dahlmanns; 15. Imaging neuropeptide release at Drosophila neuromuscular junction with a genetically engineered neuropeptide release reporter.; Yifu Han and Keke Ding; 16. Imaging synaptic glutamate release with two-photon microscopy in organotypic slice cultures; Céline D. Dürst and Thomas G. Oertner; 17. Dynole 34-2 and Acrylo-Dyn 2-30, novel dynamin GTPase chemical biology probes.; Jennifer R. Baker, Nicholas S. O’Brien, Kate L. Prichard, Phillip J. Robinson, Adam McCluskey and Cecilia C. Russell; 18. Synthesis of Pthaladyn-29 and Napthalimide-10, GTP site directed dynamin GTPase inhibitors. ; Cecilia C. Russell, Kate L. Prichard, Nicholas S. O’Brien, Adam McCluskey, Phillip J. Robinson and Jennifer R. Baker This book provides protocols on the preparation of synaptic vesicles from different model systems and provides instructions for functional investigations of their transport and formation, scaffolding, filling and priming, release, recycling, and degradation. Chapters guide readers through computational approaches, simulating different aspects of synaptic vesicle anatomy and function. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Synaptic Vesicles: Methods and Protocols aims to be a useful practical guide to researchers to help further their study in this field Neurosciences Synaptische Vesikel (DE-588)4184227-3 gnd rswk-swf Hardcover, Softcover / Medizin/Nichtklinische Fächer Synaptische Vesikel (DE-588)4184227-3 s DE-604 Dahlmanns, Jana Katharina (DE-588)1176985094 edt Dahlmanns, Marc (DE-588)1252528132 edt Erscheint auch als Online-Ausgabe 978-1-0716-1916-2 Methods in molecular biology 2417 (DE-604)BV035362695 2417 |
| spellingShingle | Synaptic vesicles methods and protocols Methods in molecular biology Neurosciences Synaptische Vesikel (DE-588)4184227-3 gnd |
| subject_GND | (DE-588)4184227-3 |
| title | Synaptic vesicles methods and protocols |
| title_auth | Synaptic vesicles methods and protocols |
| title_exact_search | Synaptic vesicles methods and protocols |
| title_exact_search_txtP | Synaptic vesicles methods and protocols |
| title_full | Synaptic vesicles methods and protocols edited by Jana Dahlmanns (Department of Psychiatry and Psychotherapy, Universitätsklinikum Erlangen, Erlangen, Bayern Germany), Marc Dahlmanns (Institute of Physiology and Pathophysiology, University of Erlangen-Nuremberg, Erlangen, Germany) |
| title_fullStr | Synaptic vesicles methods and protocols edited by Jana Dahlmanns (Department of Psychiatry and Psychotherapy, Universitätsklinikum Erlangen, Erlangen, Bayern Germany), Marc Dahlmanns (Institute of Physiology and Pathophysiology, University of Erlangen-Nuremberg, Erlangen, Germany) |
| title_full_unstemmed | Synaptic vesicles methods and protocols edited by Jana Dahlmanns (Department of Psychiatry and Psychotherapy, Universitätsklinikum Erlangen, Erlangen, Bayern Germany), Marc Dahlmanns (Institute of Physiology and Pathophysiology, University of Erlangen-Nuremberg, Erlangen, Germany) |
| title_short | Synaptic vesicles |
| title_sort | synaptic vesicles methods and protocols |
| title_sub | methods and protocols |
| topic | Neurosciences Synaptische Vesikel (DE-588)4184227-3 gnd |
| topic_facet | Neurosciences Synaptische Vesikel |
| volume_link | (DE-604)BV035362695 |
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