The role of site-specific ubiquitination of the influenza A virus polymerase:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Münster
2022
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Physical Description: | XII, 149, XXI Blätter Illustrationen, Diagramme 30 xm |
Staff View
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV048900905 | ||
| 003 | DE-604 | ||
| 005 | 00000000000000.0 | ||
| 007 | t | ||
| 008 | 230414s2022 gw a||| m||| 00||| eng d | ||
| 015 | |a 23,H01 |2 dnb | ||
| 016 | 7 | |a 1264955936 |2 DE-101 | |
| 020 | |c Broschur | ||
| 035 | |a (OCoLC)1344501098 | ||
| 035 | |a (DE-599)DNB1264955936 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 044 | |a gw |c XA-DE | ||
| 049 | |a DE-355 | ||
| 084 | |8 2\p |a 610 |2 23sdnb | ||
| 084 | |8 3\p |a 570 |2 23sdnb | ||
| 084 | |8 1\p |a 616.2 |2 23ksdnb | ||
| 100 | 1 | |a Günl, Franziska |d 1993- |e Verfasser |0 (DE-588)1268248185 |4 aut | |
| 245 | 1 | 0 | |a The role of site-specific ubiquitination of the influenza A virus polymerase |c vorgelegt von Franziska Günl |
| 264 | 1 | |a Münster |c 2022 | |
| 300 | |a XII, 149, XXI Blätter |b Illustrationen, Diagramme |c 30 xm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Wilhlems-Universität Münster |d 2022 |g Beschränkt für den Austausch | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 856 | 4 | 2 | |m B:DE-101 |q application/pdf |u https://d-nb.info/1264955936/04 |3 Inhaltsverzeichnis |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=034165320&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-034165320 | ||
| 883 | 0 | |8 1\p |a emakn |c 0,57077 |d 20221201 |q DE-101 |u https://d-nb.info/provenance/plan#emakn | |
| 883 | 2 | |8 2\p |a dnb |d 20221216 |q DE-101 |u https://d-nb.info/provenance/plan#dnb | |
| 883 | 2 | |8 3\p |a dnb |d 20221216 |q DE-101 |u https://d-nb.info/provenance/plan#dnb | |
Record in the Search Index
| _version_ | 1804185064625405952 |
|---|---|
| adam_text | TABLE
OF
CONTENT
TABLE
OF
CONTENT
LIST
OF
FIGURES
............................................................................................................................................
VI
LIST
OF
TABLES
.............................................................................................................................................
VIII
LIST
OF
EQUATIONS
.......................................................................................................................................
IX
LIST
OF
ABBREVIATIONS
...................................................................................................................................
X
1.
INTRODUCTION
..............................................................................................................................................
1
1.1.
INFLUENZA
A
VIRUS
............................................................................................................................
1
1.1.1.
TAXONOMY
OF
INFLUENZA
VIRUSES
.........................................................................................
1
1.1.2.
THE
MORPHOLOGY
OF
I
A
V
PARTICLES
......................................................................................
1
1.1.3.
HOST
RANGE
OF
I
A
V
AND
VIRAL
DETERMINANTS
FOR
SPECIES
SPECIFICITY
..................................
2
1.2.
THE
GENOME
STRUCTURE
AND
CODING
STRATEGY
OF
THE
I
AV
GENOME
...............................................
4
1.3.
VIRAL
RIBONUCLEOPROTEINS
-
THE
REPLICATION
MACHINERIES
OF
IAV
...................................................
5
1.3.1.
ARCHITECTURE
OF
RIBONUCLEOPROTEINS
...................................................................................
5
1.3.2.
THE
CONFORMATIONAL
FLEXIBILITY
OF
THE
I
A
V
POLYMERASE
.....................................................
7
1.3.3.
THE
FUNCTION
OF
VRNPS
DURING
THE
IAVLIFE
CYCLE
.............................................................
9
1.4.
POSTTRANSLATIONAL
REGULATION
OF
IAV
PROTEINS
BY
UBIQUITINATION
...................................................
14
1.4.1.
CONJUGATION
OF
UBIQUITIN
AND
UBIQUITIN-LIKE
MODIFIERS
....................................................
14
1.4.2.
THE
INFLUENCE
OF
UBIQUITINATION
ON
THE
TARGETED
PROTEIN
................................................
16
1.4.3.
THE
INFLUENCE
OF
UBIQUITIN
AND
UBL
MODIFICATION
DURING
IAV
REPLICATION
.....................
18
2.
AIM
OF
THE
THESIS
..................................................................................................................................
21
3.
MATERIALS
................................................................................................................................................
22
3.1.
CHEMICALS
AND
REAGENTS
............................................................................................................
22
3.2.
ENZYMES
......................................................................................................................................
23
3.3.
INHIBITORS
......................................................................................................................................
23
3.4.
COMMERCIAL
KITS
..........................................................................................................................24
3.5.
MEDIA
AND
BUFFERS
......................................................................................................................
24
3.5.1.
CELL
CULTURE
MEDIA,
BUFFERS
AND
SUPPLEMENTS
...............................................................
24
3.5.2.
CELL
CULTURE
MEDIA,
BUFFER
AND
SOLUTION
COMPOSITION
.....................................................
25
3.5.3.
BACTERIAL
MEDIA
COMPOSITION
.........................................................................................
26
3.5.4.
COMPOSITION
OF
BUFFERS
AND
SOLUTIONS
............................................................................
26
3.5.5.
BACTERIA
AND
CELL
LINES
....................................................................................................
28
3.5.6.
PLASMIDS
..........................................................................................................................
29
3.6.
VIRUSES
........................................................................................................................................
35
3.7.
PRIMERS
........................................................................................................................................
36
3.8.
ANTIBODIES
....................................................................................................................................
40
3.9.
HARDWARE,
EQUIPMENT
AND
DISPOSABLES
....................................................................................
40
3.10.
SOFTWARE
.....................................................................................................................................
42
TABLE
OF
CONTENT
4.
METHODS
.................................................................................................................................................
43
4.1.
CELL
CULTURE
METHODS
...................................................................................................................
43
4.1.1.
SUBCULTIVATION
OF
EUKARYOTIC
CELLS
.................................................................................43
4.1.2.
CRYOCONSERVATION
OF
CELLS
..............................................................................................
43
4.1.3.
TRANSIENT
TRANSFECTION
OF
EUKARYOTIC
CELLS
.....................................................................
43
4.1.4.
IMMUNE
FLUORESCENCE
ANALYSIS
...................................................................................
44
4.1.5.
ELECTRON
MICROSCOPY
ANALYSIS
........................................................................................
45
4.2.
VIROLOGICAL
METHODS
.....................................................................................................................45
4.2.1.
GENERATION
OF
RECOMBINANT
VIRUSES
..............................................................................
45
4.2.2.
IAV
PROPAGATION
..............................................................................................................
46
4.2.3.
I
A
V
GROWTH
KINETIC
........................................................................................................
46
4.2.4.
VIRUS
TITRATION
ASSAYS
.....................................................................................................
47
4.2.
5.
SEQUENCING
OF
VIRAL
GENOMES
.......................................................................................
48
4.2.6.
NEGATIVE
STAINING
OF
PURIFIED
VIRUS
PARTICLES
..................................................................
50
4.3.
MOLECULAR
BIOLOGICAL
METHODS
......................................................................................................
50
4.3.1.
BACTERIA
..........................................................................................................................
50
4.3.2.
DNA-TECHNIQUES
............................................................................................................
51
4.3.3.
RNA
TECHNIQUES
............................................................................................................
53
4.3.4.
MINIGENOME
ASSAYS
........................................................................................................
56
4.4.
PROTEIN
TECHNIQUES
....................................................................................................................
59
4.4.1.
GENERATION
OF
PROTEIN
LYSATES
........................................................................................
59
4.4.2.
DETERMINATION
OF
PROTEIN
CONCENTRATIONS
......................................................................
59
4.4.3.
SDS
POLYACRYLAMIDE
GEL
ELECTROPHORESIS
....................................................................
60
4.4.4.
WESTERN
BLOT
...................................................................................................................
60
4.4.5.
AFFINITY
PRECIPITATION
.......................................................................................................
61
4.4.6.
MASS
SPECTROMETRY
COUPLED
PTM-SCAN
.....................................................................
63
4.5.
IN
SITU
ASSAYS
...............................................................................................................................
64
4.5.1.
CONSERVATIONAL
ANALYSIS
................................................................................................
64
4.5.2.
HOMOLOGY
MODELING
OF
WSN
POLYMERASES
...................................................................
64
4.5.3.
MOLECULAR
DYNAMIC
SIMULATION
.......................................................................................
64
5.
RESULTS
...................................................................................................................................................
65
5.1.
UBL
MODIFICATION
OF
THE
IAV
POLYMERASE
....................................................................................
65
5.1.1.
THE
I
A
V
POLYMERASE
IS
MODIFIED
BY
DIFFERENT
UBL
TYPES
.............................................
65
5.1.2.
IDENTIFICATION
OF
THE
DI-GLYCYL
BOUND
LYSINES
WITHIN
THE
IAV
POLYMERASE
.....................
66
5.1.3.
CONSERVATIONAL
ANALYSIS
OF
THE
DI-GLYCYL
BOUND
LYSINES
...............................................
66
5.2.
CHARACTERIZING
THE
ROLE
OF
THE
SITE-SPECIFIC
UBL-MODIFICATION
FOR
IAV
POLYMERASE
ACTIVITY
....
68
5.2.1.
MUTATIONAL
SCREEN
OF
THE
PB2
SITES
.................................................................................
72
5.2.2.
MUTATIONAL
SCREEN
OF
THE
PA
SITES
...................................................................................
77
5.2.3.
MUTATIONAL
SCREEN
OF
THE
PB1
SITES
.................................................................................
82
5.3.
ELUCIDATING
THE
ROLE
OF
UBIQUITINATION
AT
PB1-K578
FOR
VIRAL
REPLICATION
....................................
85
5.3.1.
ASSESSING
THE
STABILITY
OF
RECOMBINANT
VIRUSES
ENCODING
PB
1
-K578A/R
...................
85
IV
TABLE
OF
CONTENT
5.3.2.
CHARACTERIZATION
OF
THE
INFLUENCE
OF
PB1-K578A
ON
VIRAL
REPLICATION
..........................
89
5.3.3.
CONSERVATIONAL
ANALYSIS
OF
PB1-K578
IN
IAV
ISOLATES
OF
HUMAN,
AVIAN
AND
SWINE
ORIGIN
................................................................................................................................
95
5.4.
EXAMINATION
OF
THE
STRUCTURAL
ENVIRONMENT
OF
PB1-K578
WITHIN
THE
ACTIVE
POLYMERASE
........
96
5.4.
1.
IDENTIFICATION
OF
A
CONSERVED
AND
FUNCTIONALLY
CONNECTED
INTERFACE
FORMED
BY
PB1-K578
AND
PB2-E72
..............................................................................................
97
5.4.2.
ELUCIDATING
THE
FUNCTIONAL
RELEVANCE
OF
PB2-Q73
AND
PB2-R101
FOR
THE
STABILITY
OF
THE
PB1-PB2
INTERFACE
..............................................................................................
102
5.4.3.
ASSESSING
THE
ROLE
OF
CHARGE
AT
PB1-578
FOR
POLYMERASE
DIMERIZATION
....................
104
5.5.
THE
ROLE
OF
UBIQUITINATION
AT
PB1-K578
FOR
RNA
ENCAPSIDATION
..............................................
108
5.6.
CHARACTERIZATION
OF
THE
UBIQUITIN
CHAIN
ARCHITECTURE
AT
PB1-K578
.........................................
112
6.
DISCUSSION
..........................................................................................................................................
115
6.1.
THE
INFLUENZA
A
VIRUS
POLYMERASE
IS
TARGETED
FOR
EXTENSIVE
UB/UBL-MODIFICATION
..............
115
6.2.
UB/UBL-MODIFICATION
MODULATE
IAV
POLYMERASE
FUNCTIONS
SITE-SPECIFICALLY
........................
118
6.3.
PB1-K578
IS
MODIFIED
WITH
COMPLEX
POLYUBIQUITIN
CHAINS
.......................................................
122
6.4.
UBIQUITINATION
OF
PB1-K578
IS
EXPLOITED
TO
COORDINATE
THE
SEQUENCE
OF
VRNA
REPLICATION
..
123
6.4.1.
UBIQUITINATION
AT
PB1-K578
COORDINATES
BALANCED
VRNA
SYNTHESIS
BY
REGULATING
THE
FORMATION
OF
SYMMETRIC
DIMERS
..............................................................................
124
6.4.2.
UBIQUITINATION
AT
PB1-K578
DETERMINES
NP
BINDING
TO
THE
IAVPOLYMERASE
...........
128
6.4.3.
THE
ROLE
OF
UBIQUITINATION
AT
PB1-K578
FOR
THE
TRANSITION
FROM
TRANSCRIPTASE
TO
REPLICASE
ACTIVITY
...........................................................................................................
130
7.
SUMMARY
..............................................................................................................................................
132
8.
ZUSAMMENFASSUNG
.............................................................................................................................
133
9.
REFERENCES
..........................................................................................................................................
134
10.
APPENDIX
...............................................................................................................................................
X
10.1.
ANTIBODY
TEST
UB/UBLS
...............................................................................................................
X
10.2.
BLOTS
ISG15
AND
NEDD8
IN
A549
..............................................................................................
X
10.3.
REFERENCES
FOR
THE
LINEAR
MODELS
OF
THE
POLYMERASE
SUBUNITS
...............................................
XI
10.4.
TABLES
OF
THE
IDENTIFIED
DI-GLYCYL
BOUND
LYSINES
IN
THE
IAV
POLYMERASE
................................
XIV
10.5.
DETECTION
OF
UBL
MODIFIED
LYSINES
IN
ALL
IAV
PROTEINS
...........................................................
XVI
10.6
AVIAN-TO-MAMMALIAN
ADAPTATION
MUTANTS
IN
THE
POLYMERASE
...............................................
XVII
PUBLICATIONS
AND
PRESENTATIONS
..........................................................................................................
XVIII
ACKNOWLEDGEMENTS
.................................................................................................................................
XIX
CURRICULUM
VITAE
.......................................................................................................................................
XX
EIDESSTATTLICHE
ERKLARUNG
.......................................................................................................................
XXI
|
| adam_txt |
TABLE
OF
CONTENT
TABLE
OF
CONTENT
LIST
OF
FIGURES
.
VI
LIST
OF
TABLES
.
VIII
LIST
OF
EQUATIONS
.
IX
LIST
OF
ABBREVIATIONS
.
X
1.
INTRODUCTION
.
1
1.1.
INFLUENZA
A
VIRUS
.
1
1.1.1.
TAXONOMY
OF
INFLUENZA
VIRUSES
.
1
1.1.2.
THE
MORPHOLOGY
OF
I
A
V
PARTICLES
.
1
1.1.3.
HOST
RANGE
OF
I
A
V
AND
VIRAL
DETERMINANTS
FOR
SPECIES
SPECIFICITY
.
2
1.2.
THE
GENOME
STRUCTURE
AND
CODING
STRATEGY
OF
THE
I
AV
GENOME
.
4
1.3.
VIRAL
RIBONUCLEOPROTEINS
-
THE
REPLICATION
MACHINERIES
OF
IAV
.
5
1.3.1.
ARCHITECTURE
OF
RIBONUCLEOPROTEINS
.
5
1.3.2.
THE
CONFORMATIONAL
FLEXIBILITY
OF
THE
I
A
V
POLYMERASE
.
7
1.3.3.
THE
FUNCTION
OF
VRNPS
DURING
THE
IAVLIFE
CYCLE
.
9
1.4.
POSTTRANSLATIONAL
REGULATION
OF
IAV
PROTEINS
BY
UBIQUITINATION
.
14
1.4.1.
CONJUGATION
OF
UBIQUITIN
AND
UBIQUITIN-LIKE
MODIFIERS
.
14
1.4.2.
THE
INFLUENCE
OF
UBIQUITINATION
ON
THE
TARGETED
PROTEIN
.
16
1.4.3.
THE
INFLUENCE
OF
UBIQUITIN
AND
UBL
MODIFICATION
DURING
IAV
REPLICATION
.
18
2.
AIM
OF
THE
THESIS
.
21
3.
MATERIALS
.
22
3.1.
CHEMICALS
AND
REAGENTS
.
22
3.2.
ENZYMES
.
23
3.3.
INHIBITORS
.
23
3.4.
COMMERCIAL
KITS
.24
3.5.
MEDIA
AND
BUFFERS
.
24
3.5.1.
CELL
CULTURE
MEDIA,
BUFFERS
AND
SUPPLEMENTS
.
24
3.5.2.
CELL
CULTURE
MEDIA,
BUFFER
AND
SOLUTION
COMPOSITION
.
25
3.5.3.
BACTERIAL
MEDIA
COMPOSITION
.
26
3.5.4.
COMPOSITION
OF
BUFFERS
AND
SOLUTIONS
.
26
3.5.5.
BACTERIA
AND
CELL
LINES
.
28
3.5.6.
PLASMIDS
.
29
3.6.
VIRUSES
.
35
3.7.
PRIMERS
.
36
3.8.
ANTIBODIES
.
40
3.9.
HARDWARE,
EQUIPMENT
AND
DISPOSABLES
.
40
3.10.
SOFTWARE
.
42
TABLE
OF
CONTENT
4.
METHODS
.
43
4.1.
CELL
CULTURE
METHODS
.
43
4.1.1.
SUBCULTIVATION
OF
EUKARYOTIC
CELLS
.43
4.1.2.
CRYOCONSERVATION
OF
CELLS
.
43
4.1.3.
TRANSIENT
TRANSFECTION
OF
EUKARYOTIC
CELLS
.
43
4.1.4.
IMMUNE
FLUORESCENCE
ANALYSIS
.
44
4.1.5.
ELECTRON
MICROSCOPY
ANALYSIS
.
45
4.2.
VIROLOGICAL
METHODS
.45
4.2.1.
GENERATION
OF
RECOMBINANT
VIRUSES
.
45
4.2.2.
IAV
PROPAGATION
.
46
4.2.3.
I
A
V
GROWTH
KINETIC
.
46
4.2.4.
VIRUS
TITRATION
ASSAYS
.
47
4.2.
5.
SEQUENCING
OF
VIRAL
GENOMES
.
48
4.2.6.
NEGATIVE
STAINING
OF
PURIFIED
VIRUS
PARTICLES
.
50
4.3.
MOLECULAR
BIOLOGICAL
METHODS
.
50
4.3.1.
BACTERIA
.
50
4.3.2.
DNA-TECHNIQUES
.
51
4.3.3.
RNA
TECHNIQUES
.
53
4.3.4.
MINIGENOME
ASSAYS
.
56
4.4.
PROTEIN
TECHNIQUES
.
59
4.4.1.
GENERATION
OF
PROTEIN
LYSATES
.
59
4.4.2.
DETERMINATION
OF
PROTEIN
CONCENTRATIONS
.
59
4.4.3.
SDS
POLYACRYLAMIDE
GEL
ELECTROPHORESIS
.
60
4.4.4.
WESTERN
BLOT
.
60
4.4.5.
AFFINITY
PRECIPITATION
.
61
4.4.6.
MASS
SPECTROMETRY
COUPLED
PTM-SCAN
.
63
4.5.
IN
SITU
ASSAYS
.
64
4.5.1.
CONSERVATIONAL
ANALYSIS
.
64
4.5.2.
HOMOLOGY
MODELING
OF
WSN
POLYMERASES
.
64
4.5.3.
MOLECULAR
DYNAMIC
SIMULATION
.
64
5.
RESULTS
.
65
5.1.
UBL
MODIFICATION
OF
THE
IAV
POLYMERASE
.
65
5.1.1.
THE
I
A
V
POLYMERASE
IS
MODIFIED
BY
DIFFERENT
UBL
TYPES
.
65
5.1.2.
IDENTIFICATION
OF
THE
DI-GLYCYL
BOUND
LYSINES
WITHIN
THE
IAV
POLYMERASE
.
66
5.1.3.
CONSERVATIONAL
ANALYSIS
OF
THE
DI-GLYCYL
BOUND
LYSINES
.
66
5.2.
CHARACTERIZING
THE
ROLE
OF
THE
SITE-SPECIFIC
UBL-MODIFICATION
FOR
IAV
POLYMERASE
ACTIVITY
.
68
5.2.1.
MUTATIONAL
SCREEN
OF
THE
PB2
SITES
.
72
5.2.2.
MUTATIONAL
SCREEN
OF
THE
PA
SITES
.
77
5.2.3.
MUTATIONAL
SCREEN
OF
THE
PB1
SITES
.
82
5.3.
ELUCIDATING
THE
ROLE
OF
UBIQUITINATION
AT
PB1-K578
FOR
VIRAL
REPLICATION
.
85
5.3.1.
ASSESSING
THE
STABILITY
OF
RECOMBINANT
VIRUSES
ENCODING
PB
1
-K578A/R
.
85
IV
TABLE
OF
CONTENT
5.3.2.
CHARACTERIZATION
OF
THE
INFLUENCE
OF
PB1-K578A
ON
VIRAL
REPLICATION
.
89
5.3.3.
CONSERVATIONAL
ANALYSIS
OF
PB1-K578
IN
IAV
ISOLATES
OF
HUMAN,
AVIAN
AND
SWINE
ORIGIN
.
95
5.4.
EXAMINATION
OF
THE
STRUCTURAL
ENVIRONMENT
OF
PB1-K578
WITHIN
THE
ACTIVE
POLYMERASE
.
96
5.4.
1.
IDENTIFICATION
OF
A
CONSERVED
AND
FUNCTIONALLY
CONNECTED
INTERFACE
FORMED
BY
PB1-K578
AND
PB2-E72
.
97
5.4.2.
ELUCIDATING
THE
FUNCTIONAL
RELEVANCE
OF
PB2-Q73
AND
PB2-R101
FOR
THE
STABILITY
OF
THE
PB1-PB2
INTERFACE
.
102
5.4.3.
ASSESSING
THE
ROLE
OF
CHARGE
AT
PB1-578
FOR
POLYMERASE
DIMERIZATION
.
104
5.5.
THE
ROLE
OF
UBIQUITINATION
AT
PB1-K578
FOR
RNA
ENCAPSIDATION
.
108
5.6.
CHARACTERIZATION
OF
THE
UBIQUITIN
CHAIN
ARCHITECTURE
AT
PB1-K578
.
112
6.
DISCUSSION
.
115
6.1.
THE
INFLUENZA
A
VIRUS
POLYMERASE
IS
TARGETED
FOR
EXTENSIVE
UB/UBL-MODIFICATION
.
115
6.2.
UB/UBL-MODIFICATION
MODULATE
IAV
POLYMERASE
FUNCTIONS
SITE-SPECIFICALLY
.
118
6.3.
PB1-K578
IS
MODIFIED
WITH
COMPLEX
POLYUBIQUITIN
CHAINS
.
122
6.4.
UBIQUITINATION
OF
PB1-K578
IS
EXPLOITED
TO
COORDINATE
THE
SEQUENCE
OF
VRNA
REPLICATION
.
123
6.4.1.
UBIQUITINATION
AT
PB1-K578
COORDINATES
BALANCED
VRNA
SYNTHESIS
BY
REGULATING
THE
FORMATION
OF
SYMMETRIC
DIMERS
.
124
6.4.2.
UBIQUITINATION
AT
PB1-K578
DETERMINES
NP
BINDING
TO
THE
IAVPOLYMERASE
.
128
6.4.3.
THE
ROLE
OF
UBIQUITINATION
AT
PB1-K578
FOR
THE
TRANSITION
FROM
TRANSCRIPTASE
TO
REPLICASE
ACTIVITY
.
130
7.
SUMMARY
.
132
8.
ZUSAMMENFASSUNG
.
133
9.
REFERENCES
.
134
10.
APPENDIX
.
X
10.1.
ANTIBODY
TEST
UB/UBLS
.
X
10.2.
BLOTS
ISG15
AND
NEDD8
IN
A549
.
X
10.3.
REFERENCES
FOR
THE
LINEAR
MODELS
OF
THE
POLYMERASE
SUBUNITS
.
XI
10.4.
TABLES
OF
THE
IDENTIFIED
DI-GLYCYL
BOUND
LYSINES
IN
THE
IAV
POLYMERASE
.
XIV
10.5.
DETECTION
OF
UBL
MODIFIED
LYSINES
IN
ALL
IAV
PROTEINS
.
XVI
10.6
AVIAN-TO-MAMMALIAN
ADAPTATION
MUTANTS
IN
THE
POLYMERASE
.
XVII
PUBLICATIONS
AND
PRESENTATIONS
.
XVIII
ACKNOWLEDGEMENTS
.
XIX
CURRICULUM
VITAE
.
XX
EIDESSTATTLICHE
ERKLARUNG
.
XXI |
| any_adam_object | 1 |
| any_adam_object_boolean | 1 |
| author | Günl, Franziska 1993- |
| author_GND | (DE-588)1268248185 |
| author_facet | Günl, Franziska 1993- |
| author_role | aut |
| author_sort | Günl, Franziska 1993- |
| author_variant | f g fg |
| building | Verbundindex |
| bvnumber | BV048900905 |
| ctrlnum | (OCoLC)1344501098 (DE-599)DNB1264955936 |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01744nam a2200409 c 4500</leader><controlfield tag="001">BV048900905</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">230414s2022 gw a||| m||| 00||| eng d</controlfield><datafield tag="015" ind1=" " ind2=" "><subfield code="a">23,H01</subfield><subfield code="2">dnb</subfield></datafield><datafield tag="016" ind1="7" ind2=" "><subfield code="a">1264955936</subfield><subfield code="2">DE-101</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="c">Broschur</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)1344501098</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DNB1264955936</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="044" ind1=" " ind2=" "><subfield code="a">gw</subfield><subfield code="c">XA-DE</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="8">2\p</subfield><subfield code="a">610</subfield><subfield code="2">23sdnb</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="8">3\p</subfield><subfield code="a">570</subfield><subfield code="2">23sdnb</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="8">1\p</subfield><subfield code="a">616.2</subfield><subfield code="2">23ksdnb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Günl, Franziska</subfield><subfield code="d">1993-</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)1268248185</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">The role of site-specific ubiquitination of the influenza A virus polymerase</subfield><subfield code="c">vorgelegt von Franziska Günl</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Münster</subfield><subfield code="c">2022</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">XII, 149, XXI Blätter</subfield><subfield code="b">Illustrationen, Diagramme</subfield><subfield code="c">30 xm</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="b">Dissertation</subfield><subfield code="c">Wilhlems-Universität Münster</subfield><subfield code="d">2022</subfield><subfield code="g">Beschränkt für den Austausch</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">B:DE-101</subfield><subfield code="q">application/pdf</subfield><subfield code="u">https://d-nb.info/1264955936/04</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=034165320&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-034165320</subfield></datafield><datafield tag="883" ind1="0" ind2=" "><subfield code="8">1\p</subfield><subfield code="a">emakn</subfield><subfield code="c">0,57077</subfield><subfield code="d">20221201</subfield><subfield code="q">DE-101</subfield><subfield code="u">https://d-nb.info/provenance/plan#emakn</subfield></datafield><datafield tag="883" ind1="2" ind2=" "><subfield code="8">2\p</subfield><subfield code="a">dnb</subfield><subfield code="d">20221216</subfield><subfield code="q">DE-101</subfield><subfield code="u">https://d-nb.info/provenance/plan#dnb</subfield></datafield><datafield tag="883" ind1="2" ind2=" "><subfield code="8">3\p</subfield><subfield code="a">dnb</subfield><subfield code="d">20221216</subfield><subfield code="q">DE-101</subfield><subfield code="u">https://d-nb.info/provenance/plan#dnb</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV048900905 |
| illustrated | Illustrated |
| index_date | 2024-07-03T21:50:57Z |
| indexdate | 2024-07-10T09:49:18Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-034165320 |
| oclc_num | 1344501098 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | XII, 149, XXI Blätter Illustrationen, Diagramme 30 xm |
| publishDate | 2022 |
| publishDateSearch | 2022 |
| publishDateSort | 2022 |
| record_format | marc |
| spelling | Günl, Franziska 1993- Verfasser (DE-588)1268248185 aut The role of site-specific ubiquitination of the influenza A virus polymerase vorgelegt von Franziska Günl Münster 2022 XII, 149, XXI Blätter Illustrationen, Diagramme 30 xm txt rdacontent n rdamedia nc rdacarrier Dissertation Wilhlems-Universität Münster 2022 Beschränkt für den Austausch (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf https://d-nb.info/1264955936/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=034165320&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p emakn 0,57077 20221201 DE-101 https://d-nb.info/provenance/plan#emakn 2\p dnb 20221216 DE-101 https://d-nb.info/provenance/plan#dnb 3\p dnb 20221216 DE-101 https://d-nb.info/provenance/plan#dnb |
| spellingShingle | Günl, Franziska 1993- The role of site-specific ubiquitination of the influenza A virus polymerase |
| subject_GND | (DE-588)4113937-9 |
| title | The role of site-specific ubiquitination of the influenza A virus polymerase |
| title_auth | The role of site-specific ubiquitination of the influenza A virus polymerase |
| title_exact_search | The role of site-specific ubiquitination of the influenza A virus polymerase |
| title_exact_search_txtP | The role of site-specific ubiquitination of the influenza A virus polymerase |
| title_full | The role of site-specific ubiquitination of the influenza A virus polymerase vorgelegt von Franziska Günl |
| title_fullStr | The role of site-specific ubiquitination of the influenza A virus polymerase vorgelegt von Franziska Günl |
| title_full_unstemmed | The role of site-specific ubiquitination of the influenza A virus polymerase vorgelegt von Franziska Günl |
| title_short | The role of site-specific ubiquitination of the influenza A virus polymerase |
| title_sort | the role of site specific ubiquitination of the influenza a virus polymerase |
| topic_facet | Hochschulschrift |
| url | https://d-nb.info/1264955936/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=034165320&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT gunlfranziska theroleofsitespecificubiquitinationoftheinfluenzaaviruspolymerase |
There is no print copy available.
Indexes