The immunological synapse: Part A
Saved in:
| Other Authors: | , |
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| Format: | Book |
| Language: | English |
| Published: |
Cambridge, MA ; San Diego, CA ; Oxford ; London
Academic Press, an imprint of Elsevier
2023
|
| Edition: | First edition |
| Series: | Methods in cell biology
volume 173 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | xv, 189 Seiten Illustrationen, Diagramme |
| ISBN: | 9780323901543 |
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Record in the Search Index
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| adam_text | Contents Contributors............................................................................................................. xi CHAPTER 1 Imaging polarized granule release at the cytotoxic T cell immunological synapse using TIRF microscopy: Control by polarity regulators........ 1 Marie Juzans, Céline Cuche, Vincenzo Di Bartolo, and Andrés Alcover 1. Introduction.................................................................................... 2 2. Materials......................................................................................... 4 3. Methods.......................................................................................... 5 4. Concluding remarks...................................................................... 11 5. Notes............................................................................................. 11 Acknowledgments.............................................................................. 12 Declaration of interests...................................................................... 12 References.......................................................................................... 12 CHAPTER 2 Analysis of centrosoma! area actin reorganization and centrosome polarization upon lymphocyte activation at the immunological synapse............................................ 15 Sara Fernandez-Hermira, Irene Sanz-Fernández, Marta Botas, Victor Calvo, and Manuel Izquierdo 1. Introduction................................................................................... 17 2. Materials, cells, immunological synapse
formation and image capture............................................................................... 20 3. Imaging the immunological synapse........................................... 21 4. Discussion, future perspectivesand concluding remarks............. 25 5, Notes............................................................................................. 27 Acknowledgments............................................................................. 29 Conflict of interest............................................................................. 29 Author contributions.......................................................................... 29 References......................................................................................... 29 V
vi Contents CHAPTER З P815-based redirected degranulation assay to study human NK cell effector functions.33 Iñigo Terren, Gabirel Astarloa-Pando, Ainhoa Amarilla-lrusta, and Francisco Borrego Introduction................................................................................... 34 Prior to the assay........................................................................... 37 Redirected degranulation assay.................................................... 37 Flow cytometry staining................................................................38 Analyzing CD16-induced effector functions of NK cells........... 41 Concluding remarks...................................................................... 44 Notes.............................................................................................. 44 Acknowledgments .............................................................................. 46 Conflict of interests............................................................................ 46 References...........................................................................................46 1. 2. 3. 4. 5. 6. 7. CHAPTER 4 Cytotoxic and chemotactic dynamicsof NK cells quantified by live-cell imaging............................... 49 Yanting Zhu and Jue Shi Introduction.................................................................................. 50 Materials...................................................................................... 51 Methods........................................................................................53
Notes............................................................................................ 62 Acknowledgments .............................................................................. 63 References...........................................................................................63 1. 2. 3. 4. CHAPTER 5 Quantification of interaction frequencybetween antigen-presenting cells and T cells by conjugationassay................................................... 65 Ondrej Cerny 1. Introduction.................................................................................. 66 2. Materials...................................................................................... 67 3. Methods........................................................................................ 68 4. Concluding remarks..................................................................... 72 5. Notes............................................................................................ 72 Acknowledgment............................................................................... 74 References.......................................................................................... 74
Contents CHAPTER 6 Assessment of membrane lipid state at the natural killer cell immunological synapse.............. 77 Yu Li and Jordan S. Orange 1. 2. 3. 4. 5. Introduction.................................................................................. 78 Materials....................................................................................... 80 Common procedures.................................................................... 81 Assessment of membrane lipid state via confocal microscopy...82 Assessment of membrane lipid state via total internal reflection fluorescence microscopy............................................ 84 6. Dataanalysis................................................................................ 86 7. Notes............................................................................................. 87 Acknowledgments............................................................................. 88 References......................................................................................... 88 CHAPTER 7 Study of the effects of NK-tumor cell interaction by proteomic analysis and imaging.... 91 Chiara Lavarello, Paola Orecchia, Andrea Petretto, Massimo Vitale, Claudia Cantoni, and Monica Parodi 1. 2. 3. 4. Introduction................................................................................. 93 Melanoma/NK cellco-culture...................................................... 93 Cell imaging................................................................................ 97 Analysis of the proteomic changes related to the process
of EMT inducedby NK cells.......................................................99 Notes................................................................................................ 105 Acknowledgments ............................................................................ 106 Declaration of interests.................................................................... 107 References........................................................................................ 107 CHAPTER 8 Protocol for the murine antibody-dependent cellular phagocytosis assay............. 109 Eliana Stanganello, Magdalena Brkic, Steven Zenner, Ines Beulshausen, Ute Schmitt, and Fulvia Vascotto 1. Introduction.................................................................................HO 2. Materials..................................................................................... 112 3. Notes........................................................................................... US Acknowledgment............................................................................. 118 Author disclosure............................................................................. 118 References........................................................................................ 118
viii Contents CHAPTER 9 Quantification oflymphocyticchoriomeningitis virus specificT cells andLCMV viral titers........... 121 Melanie Grusdat, Catherine Dostert, and Dirk Brenner 1. Introduction................................................................................... 122 2. Materials........................................................................................ 123 3. Methods......................................................................................... 124 4. Notes.............................................................................................. 129 5. Conclusion..................................................................................... 129 Acknowledgments............................................................................. 130 References......................................................................................... 130 CHAPTER 10 An in vitro model to monitor natural killer cell effector functions against breast cancer cells derived from human tumor tissue...... 133 Nicky A. Beelen, Femke A.I. Ehlers, Loes F.S. Kooreman, Gerard M.J. Bos, and Lotte Wieten 1. Introduction..................................................................................135 2. Before you begin......................................................................... 137 3. Key resources table..................................................................... 138 4. Materials and equipment............................................................. 139 5. Step-by-step method
details........................................................ 140 6. Expected outcomes...................................................................... 148 7. Quantification and statistical analysis........................................ 148 8. Advantages................................................................................... 151 9. Limitations................................................................................... 151 10. Safety considerations andstandards............................................ 151 References.............................................................................................152 CHAPTER 11 Standardized protocol for the evaluation of chimeric antigen receptor (CAR)-modified cell immunological synapse quality using the glasssupported planar lipid bilayer........... 155 Jong Hyun Cho, Wei-chung Tsao, Alireza Naghizadeh, and Dongfang Liu 1. 2. 3. 4. Introduction................................................................................... 156 Before you begin...........................................................................158 Key resources table....................................................................... 159 Materials and equipment.............................................................. 160
Contents Step-by-step method details.......................................................161 Expected outcomes.................................................................... 167 7. Statistical analysis...................................................................... 169 8. Limitations.................................................................................. 169 References......................................................................................... 170 5. 6. CHAPTER 12 Potency monitoring of CAR T cells........................ 173 Dongrui Wang, Xin Yang, Agata Xella, Lawrence A. Stern, and Christine E. Brown 1. Introduction.................................................................................. 174 2. Preparation of cells...................................................................... 175 3. Setup co-culture for extended long-term killing (ELTK) assay (Assay #1).................................................................... 176 4. Setup co-culture for re-challengeassay (Assay #2).................... 178 5. General procedures of flowcytometry analysis......................... 179 6. Analysis of CAR T cell killing and T cell counts by flow cytometry................................................................................ 179 7. Analysis of CAR T cell phenotypes by flow cytometry.............181 8. Quantification of cytokine productionby CAR T cells.............. 185 9. Concluding remarks..................................................................... 185 10.
Notes............................................................................................ 186 References........................................................................................... 187 ix
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| adam_txt |
Contents Contributors. xi CHAPTER 1 Imaging polarized granule release at the cytotoxic T cell immunological synapse using TIRF microscopy: Control by polarity regulators. 1 Marie Juzans, Céline Cuche, Vincenzo Di Bartolo, and Andrés Alcover 1. Introduction. 2 2. Materials. 4 3. Methods. 5 4. Concluding remarks. 11 5. Notes. 11 Acknowledgments. 12 Declaration of interests. 12 References. 12 CHAPTER 2 Analysis of centrosoma! area actin reorganization and centrosome polarization upon lymphocyte activation at the immunological synapse. 15 Sara Fernandez-Hermira, Irene Sanz-Fernández, Marta Botas, Victor Calvo, and Manuel Izquierdo 1. Introduction. 17 2. Materials, cells, immunological synapse
formation and image capture. 20 3. Imaging the immunological synapse. 21 4. Discussion, future perspectivesand concluding remarks. 25 5, Notes. 27 Acknowledgments. 29 Conflict of interest. 29 Author contributions. 29 References. 29 V
vi Contents CHAPTER З P815-based redirected degranulation assay to study human NK cell effector functions.33 Iñigo Terren, Gabirel Astarloa-Pando, Ainhoa Amarilla-lrusta, and Francisco Borrego Introduction. 34 Prior to the assay. 37 Redirected degranulation assay. 37 Flow cytometry staining.38 Analyzing CD16-induced effector functions of NK cells. 41 Concluding remarks. 44 Notes. 44 Acknowledgments . 46 Conflict of interests. 46 References.46 1. 2. 3. 4. 5. 6. 7. CHAPTER 4 Cytotoxic and chemotactic dynamicsof NK cells quantified by live-cell imaging. 49 Yanting Zhu and Jue Shi Introduction. 50 Materials. 51 Methods.53
Notes. 62 Acknowledgments . 63 References.63 1. 2. 3. 4. CHAPTER 5 Quantification of interaction frequencybetween antigen-presenting cells and T cells by conjugationassay. 65 Ondrej Cerny 1. Introduction. 66 2. Materials. 67 3. Methods. 68 4. Concluding remarks. 72 5. Notes. 72 Acknowledgment. 74 References. 74
Contents CHAPTER 6 Assessment of membrane lipid state at the natural killer cell immunological synapse. 77 Yu Li and Jordan S. Orange 1. 2. 3. 4. 5. Introduction. 78 Materials. 80 Common procedures. 81 Assessment of membrane lipid state via confocal microscopy.82 Assessment of membrane lipid state via total internal reflection fluorescence microscopy. 84 6. Dataanalysis. 86 7. Notes. 87 Acknowledgments. 88 References. 88 CHAPTER 7 Study of the effects of NK-tumor cell interaction by proteomic analysis and imaging. 91 Chiara Lavarello, Paola Orecchia, Andrea Petretto, Massimo Vitale, Claudia Cantoni, and Monica Parodi 1. 2. 3. 4. Introduction. 93 Melanoma/NK cellco-culture. 93 Cell imaging. 97 Analysis of the proteomic changes related to the process
of EMT inducedby NK cells.99 Notes. 105 Acknowledgments . 106 Declaration of interests. 107 References. 107 CHAPTER 8 Protocol for the murine antibody-dependent cellular phagocytosis assay. 109 Eliana Stanganello, Magdalena Brkic, Steven Zenner, Ines Beulshausen, Ute Schmitt, and Fulvia Vascotto 1. Introduction.HO 2. Materials. 112 3. Notes. US Acknowledgment. 118 Author disclosure. 118 References. 118
viii Contents CHAPTER 9 Quantification oflymphocyticchoriomeningitis virus specificT cells andLCMV viral titers. 121 Melanie Grusdat, Catherine Dostert, and Dirk Brenner 1. Introduction. 122 2. Materials. 123 3. Methods. 124 4. Notes. 129 5. Conclusion. 129 Acknowledgments. 130 References. 130 CHAPTER 10 An in vitro model to monitor natural killer cell effector functions against breast cancer cells derived from human tumor tissue. 133 Nicky A. Beelen, Femke A.I. Ehlers, Loes F.S. Kooreman, Gerard M.J. Bos, and Lotte Wieten 1. Introduction.135 2. Before you begin. 137 3. Key resources table. 138 4. Materials and equipment. 139 5. Step-by-step method
details. 140 6. Expected outcomes. 148 7. Quantification and statistical analysis. 148 8. Advantages. 151 9. Limitations. 151 10. Safety considerations andstandards. 151 References.152 CHAPTER 11 Standardized protocol for the evaluation of chimeric antigen receptor (CAR)-modified cell immunological synapse quality using the glasssupported planar lipid bilayer. 155 Jong Hyun Cho, Wei-chung Tsao, Alireza Naghizadeh, and Dongfang Liu 1. 2. 3. 4. Introduction. 156 Before you begin.158 Key resources table. 159 Materials and equipment. 160
Contents Step-by-step method details.161 Expected outcomes. 167 7. Statistical analysis. 169 8. Limitations. 169 References. 170 5. 6. CHAPTER 12 Potency monitoring of CAR T cells. 173 Dongrui Wang, Xin Yang, Agata Xella, Lawrence A. Stern, and Christine E. Brown 1. Introduction. 174 2. Preparation of cells. 175 3. Setup co-culture for extended long-term killing (ELTK) assay (Assay #1). 176 4. Setup co-culture for re-challengeassay (Assay #2). 178 5. General procedures of flowcytometry analysis. 179 6. Analysis of CAR T cell killing and T cell counts by flow cytometry. 179 7. Analysis of CAR T cell phenotypes by flow cytometry.181 8. Quantification of cytokine productionby CAR T cells. 185 9. Concluding remarks. 185 10.
Notes. 186 References. 187 ix |
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| spelling | Thomas, Clément edt The immunological synapse Part A edited by Clément Thomas (Luxembourg Institute of Health, Luxembourg City, Luxembourg), Lorenzo Galluzzi (Weill Cornell Medicine, New York, NY, United States) First edition Cambridge, MA ; San Diego, CA ; Oxford ; London Academic Press, an imprint of Elsevier 2023 xv, 189 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Methods in cell biology volume 173 Methods in cell biology (DE-588)4143413-4 Aufsatzsammlung gnd-content Galluzzi, Lorenzo 1980- (DE-588)112867842X edt (DE-604)BV048807812 A Erscheint auch als Online-Ausgabe 978-0-323-90155-0 Methods in cell biology volume 173 (DE-604)BV002534878 173 Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=034073828&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
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| title | The immunological synapse |
| title_auth | The immunological synapse |
| title_exact_search | The immunological synapse |
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| title_full | The immunological synapse Part A edited by Clément Thomas (Luxembourg Institute of Health, Luxembourg City, Luxembourg), Lorenzo Galluzzi (Weill Cornell Medicine, New York, NY, United States) |
| title_fullStr | The immunological synapse Part A edited by Clément Thomas (Luxembourg Institute of Health, Luxembourg City, Luxembourg), Lorenzo Galluzzi (Weill Cornell Medicine, New York, NY, United States) |
| title_full_unstemmed | The immunological synapse Part A edited by Clément Thomas (Luxembourg Institute of Health, Luxembourg City, Luxembourg), Lorenzo Galluzzi (Weill Cornell Medicine, New York, NY, United States) |
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