Konzert Nr. III für Marimba & Kammer Ensemble: (Linzer) : 2003
Saved in:
| Main Author: | |
|---|---|
| Format: | Electronic Musical Score Book |
| Language: | English |
| Published: |
Copenhagen
Wilhelm Hansen
[2003]
|
| Edition: | [Set of parts] |
| Subjects: | |
| Online Access: | BSB01 Volltext Inhaltsverzeichnis |
| Item Description: | Title from resource description page (viewed February 08, 2018) Written in 2002 |
| Physical Description: | 1 Online-Ressource (6 Teils) |
Staff View
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| 505 | 8 | 0 | |t Pianoforte -- |t Violino I -- |t Violino II -- |t Violoncello -- |t Contrabasso -- |t Percussione |
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Record in the Search Index
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| adam_text | TABLE OF CONTENTS
TABLE OF CONTENTS
1. SUMMARY 1
2. ZUSAMMENFASSUNG 3
3. INTRODUCTION 5
3.1 AP-1 PROTEINS 5
3.2 REGULATION OF C-JUN
PHOSPHORYLATION 8
3.3 MODULATORS OF C-JUN ACTIVITY 11
3.3.1 NEGATIVE MODULATORS OF C-JUN BIOLOGICAL ACTIVITY 11
3.3.2 POSITIVE MODULATORS OF C-JUN BIOLOGICAL ACTIVITY 18
3.4 ROLE
OF C-JUN IN CELLULAR PROLIFERATION 22
3.5 ROLE OF C-JUN IN CENTRAL NERVOUS SYSTEM 24
3.5.1 ROLE
OF C-JUN IN NEURONAL CELL DEATH 25
3.5.2 ROLE
OF C-JUN IN NEURONAL REGENERATION 29
3.5.3 ROLE OF C-JUN IN NEURONAL INFLAMMATION 33
4. MATERIALS
AND METHODS 35
4.1 DEVISE MANUFACTURERS 35
4.2 METHODS 44
4.2.1 ANIMALS 44
4.2.2 CELL CULTURE 44
4.2.2. A CULTURES OF CEREBELLAR GRANULAR NEURONS 44
4.2.2.B CULTURES OF CEREBELLAR NEURONAL/GLIAL CELLS 45
4.2.2
.C
CULTURES OF CEREBRAL MICROGLIAL CELLS 45
4.2.2.D PC12 CELL CULTURES 45
4.2.2.E CELL DEATH AND SURVIVAL ASSAY 46
4.2.2.F IMMUNOCYTOCHEMISTRY 46
4.2.2.G IMMUNOHISTOCHEMISTRY 47
I
HTTP://D-NB.INFO/1048445755
TABLE OF CONTENTS
4.2.3 MOLECULAR BIOLOGY TECHNIQUES 48
4.2.3.A MOLECULAR CLONING 48
4.2.3.B PRODUCTION OF LENTIVIRAL PARTICLES 51
4.2.3
.C
ISOLATION OF TOTAL RNA 53
4.2.3.D REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION 53
4.2.3.E QUANTITATIVE
REALTIME PCR 54
4.2.3.F GENOTYPING OF C-JUN KNOCK OUT MICE 54
4.2.4 PROTEIN BIOCHEMISTRY 58
4.3.4.A PURIFICATION OF RECOMBINANT PROTEINS 58
4.3.4.B IN VITRO KINASE
ASSAY 59
4.3.4
.C
IMMUNOPRECIPITATION 60
4.3.4.D WESTERN
BLOT 60
4.3.4.E LUCIFERASE ASSAY 61
4.3.4.F IL-1P IMMUNODETECTION BY ELISA 61
4.3.4.G MASS SPECTROMETRY AND PROTEIN IDENTIFICATION 63
5. OBJECTIVE OF WORK 65
5. RESULTS 67
6.1 ROLE
OF C-JUN IN NEURONAL CELL DEATH 67
6.1.1 PHOSPHORYLATION OF C-JUN N-TERMINAL DOMAIN IN CEREBELLAR
GRANULE NEURONS 67
6.1.2 LACK OF T91/93 PHOSPHORYLATION IMPAIRS CELL DEATH BUT NOT
NEURITEOUTGROWTH 73
6.1.3 DEREGULATION OF C-JUN PHOSPHORYLATION AT THE
T91/T93 SITES RECOVERS CELL DEATH IN PRESENCE OF LITHIUM 83
6.1.4 THE
T95 SITE OF C-JUN IS PHOSPHORYLATED IN VIVO 87
6.1.5 JNK-1 PHOSPHORYLATES T91/93 RESIDUES OF RECOMBINANT
C-JUN PROTEINS IN VITRO 93
TABLE OF CONTENTS
6.2 ROLE OF C-JUN
IN NEURONAL INFLAMMATION 99
6.2.1 LPS
ELICITS JNK-DEPENDENT PHOSPHORYLATION OF C-JUN IN BERGEMANN
GLIA CELLS 99
6.2.2 BERGMANN GLIA EXPRESS TLR4 CELLS 104
6.2.3 C-JUN
IS REQUIRED FOR 11-1
EXPRESSION IN LPS-ACTIVATED
BERGMANN GLIAL CELLS 106
6.2.4 C-JUN
IS EXPRESSED DURING FORMATION OF THE BERGMANN GLIAL MONOLAYER 110
6.2.5 C-JUN EXPRESSION
IN PURKINJE CELLS AND GRANULE NEURONS 112
6. DISCUSSION 117
7.1 DIFFERENTIAL PHOSPHORYLATION OF N-TERMINAL C-JUN IN NEURONAL CELLS.
117
7.2 IN VIVO AND IN VITRO PHOSPHORYLATION OF C-JUN AT T95-T93-T91 119
7.3 ROLE
OF C-JUN IN LPS-MEDIATED ACTIVATION OF BERGMANN GLIAL CELLS 120
7.5. C-JUN
IS REQUIRED FOR LPS-INDUCED EXPRESSION OF 11-1
P 121
7.6. IN VIVO EXPRESSION OF C-JUN IN THE POSTNATAL CEREBELLUM 121
8. REFERENCES 123
9. LIST
OF FIGURES AND TABLES 148
10. ABBREVIATIONS 150
11. AFFIDAVIT 152
12. CURRICULUM VITAE 153
13. ACKNOWLEDGEMENTS 156
III
|
| adam_txt |
TABLE OF CONTENTS
TABLE OF CONTENTS
1. SUMMARY 1
2. ZUSAMMENFASSUNG 3
3. INTRODUCTION 5
3.1 AP-1 PROTEINS 5
3.2 REGULATION OF C-JUN
PHOSPHORYLATION 8
3.3 MODULATORS OF C-JUN ACTIVITY 11
3.3.1 NEGATIVE MODULATORS OF C-JUN BIOLOGICAL ACTIVITY 11
3.3.2 POSITIVE MODULATORS OF C-JUN BIOLOGICAL ACTIVITY 18
3.4 ROLE
OF C-JUN IN CELLULAR PROLIFERATION 22
3.5 ROLE OF C-JUN IN CENTRAL NERVOUS SYSTEM 24
3.5.1 ROLE
OF C-JUN IN NEURONAL CELL DEATH 25
3.5.2 ROLE
OF C-JUN IN NEURONAL REGENERATION 29
3.5.3 ROLE OF C-JUN IN NEURONAL INFLAMMATION 33
4. MATERIALS
AND METHODS 35
4.1 DEVISE MANUFACTURERS 35
4.2 METHODS 44
4.2.1 ANIMALS 44
4.2.2 CELL CULTURE 44
4.2.2. A CULTURES OF CEREBELLAR GRANULAR NEURONS 44
4.2.2.B CULTURES OF CEREBELLAR NEURONAL/GLIAL CELLS 45
4.2.2
.C
CULTURES OF CEREBRAL MICROGLIAL CELLS 45
4.2.2.D PC12 CELL CULTURES 45
4.2.2.E CELL DEATH AND SURVIVAL ASSAY 46
4.2.2.F IMMUNOCYTOCHEMISTRY 46
4.2.2.G IMMUNOHISTOCHEMISTRY 47
I
HTTP://D-NB.INFO/1048445755
TABLE OF CONTENTS
4.2.3 MOLECULAR BIOLOGY TECHNIQUES 48
4.2.3.A MOLECULAR CLONING 48
4.2.3.B PRODUCTION OF LENTIVIRAL PARTICLES 51
4.2.3
.C
ISOLATION OF TOTAL RNA 53
4.2.3.D REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION 53
4.2.3.E QUANTITATIVE
REALTIME PCR 54
4.2.3.F GENOTYPING OF C-JUN KNOCK OUT MICE 54
4.2.4 PROTEIN BIOCHEMISTRY 58
4.3.4.A PURIFICATION OF RECOMBINANT PROTEINS 58
4.3.4.B IN VITRO KINASE
ASSAY 59
4.3.4
.C
IMMUNOPRECIPITATION 60
4.3.4.D WESTERN
BLOT 60
4.3.4.E LUCIFERASE ASSAY 61
4.3.4.F IL-1P IMMUNODETECTION BY ELISA 61
4.3.4.G MASS SPECTROMETRY AND PROTEIN IDENTIFICATION 63
5. OBJECTIVE OF WORK 65
5. RESULTS 67
6.1 ROLE
OF C-JUN IN NEURONAL CELL DEATH 67
6.1.1 PHOSPHORYLATION OF C-JUN N-TERMINAL DOMAIN IN CEREBELLAR
GRANULE NEURONS 67
6.1.2 LACK OF T91/93 PHOSPHORYLATION IMPAIRS CELL DEATH BUT NOT
NEURITEOUTGROWTH 73
6.1.3 DEREGULATION OF C-JUN PHOSPHORYLATION AT THE
T91/T93 SITES RECOVERS CELL DEATH IN PRESENCE OF LITHIUM 83
6.1.4 THE
T95 SITE OF C-JUN IS PHOSPHORYLATED IN VIVO 87
6.1.5 JNK-1 PHOSPHORYLATES T91/93 RESIDUES OF RECOMBINANT
C-JUN PROTEINS IN VITRO 93
TABLE OF CONTENTS
6.2 ROLE OF C-JUN
IN NEURONAL INFLAMMATION 99
6.2.1 LPS
ELICITS JNK-DEPENDENT PHOSPHORYLATION OF C-JUN IN BERGEMANN
GLIA CELLS 99
6.2.2 BERGMANN GLIA EXPRESS TLR4 CELLS 104
6.2.3 C-JUN
IS REQUIRED FOR 11-1
EXPRESSION IN LPS-ACTIVATED
BERGMANN GLIAL CELLS 106
6.2.4 C-JUN
IS EXPRESSED DURING FORMATION OF THE BERGMANN GLIAL MONOLAYER 110
6.2.5 C-JUN EXPRESSION
IN PURKINJE CELLS AND GRANULE NEURONS 112
6. DISCUSSION 117
7.1 DIFFERENTIAL PHOSPHORYLATION OF N-TERMINAL C-JUN IN NEURONAL CELLS.
117
7.2 IN VIVO AND IN VITRO PHOSPHORYLATION OF C-JUN AT T95-T93-T91 119
7.3 ROLE
OF C-JUN IN LPS-MEDIATED ACTIVATION OF BERGMANN GLIAL CELLS 120
7.5. C-JUN
IS REQUIRED FOR LPS-INDUCED EXPRESSION OF 11-1
P 121
7.6. IN VIVO EXPRESSION OF C-JUN IN THE POSTNATAL CEREBELLUM 121
8. REFERENCES 123
9. LIST
OF FIGURES AND TABLES 148
10. ABBREVIATIONS 150
11. AFFIDAVIT 152
12. CURRICULUM VITAE 153
13. ACKNOWLEDGEMENTS 156
III |
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| illustrated | Not Illustrated |
| index_date | 2024-07-03T20:28:33Z |
| indexdate | 2024-07-10T09:38:12Z |
| institution | BVB |
| language | English |
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| publisher | Wilhelm Hansen |
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| spelling | Koppel, Anders 1947- cmp Concertos marimba, orchestra no. 3 Konzert Nr. III für Marimba & Kammer Ensemble (Linzer) : 2003 Anders Koppel Linzer [Set of parts] Copenhagen Wilhelm Hansen [2003] 1 Online-Ressource (6 Teils) ntm rdacontent c rdamedia cr rdacarrier Title from resource description page (viewed February 08, 2018) Written in 2002 Pianoforte -- Violino I -- Violino II -- Violoncello -- Contrabasso -- Percussione Concertos (Marimba with orchestra) Parts Concertos Parts (Music) http://www.aspresolver.com/aspresolver.asp?SHM4;2451324 Verlag URL des Erstveröffentlichers Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=033819173&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis data file rda piano 1 violin 2 cello 1 double bass 1 percussion 1 lcmpt |
| spellingShingle | Koppel, Anders 1947- Konzert Nr. III für Marimba & Kammer Ensemble (Linzer) : 2003 Pianoforte -- Violino I -- Violino II -- Violoncello -- Contrabasso -- Percussione Concertos (Marimba with orchestra) Parts |
| title | Konzert Nr. III für Marimba & Kammer Ensemble (Linzer) : 2003 |
| title_alt | Concertos Linzer Pianoforte -- Violino I -- Violino II -- Violoncello -- Contrabasso -- Percussione |
| title_auth | Konzert Nr. III für Marimba & Kammer Ensemble (Linzer) : 2003 |
| title_exact_search | Konzert Nr. III für Marimba & Kammer Ensemble (Linzer) : 2003 |
| title_exact_search_txtP | Konzert Nr. III für Marimba & Kammer Ensemble (Linzer) : 2003 |
| title_full | Konzert Nr. III für Marimba & Kammer Ensemble (Linzer) : 2003 Anders Koppel |
| title_fullStr | Konzert Nr. III für Marimba & Kammer Ensemble (Linzer) : 2003 Anders Koppel |
| title_full_unstemmed | Konzert Nr. III für Marimba & Kammer Ensemble (Linzer) : 2003 Anders Koppel |
| title_short | Konzert Nr. III für Marimba & Kammer Ensemble |
| title_sort | konzert nr iii fur marimba kammer ensemble linzer 2003 |
| title_sub | (Linzer) : 2003 |
| topic | Concertos (Marimba with orchestra) Parts |
| topic_facet | Concertos (Marimba with orchestra) Parts |
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