Detection of cell death mechanisms: methods and protocols
This volume provides detailed protocols for the performance, analysis, and troubleshooting of in vitro and in vivo experiments related to programmed cell death. Chapters compile conventional techniques such as western blot and qPCR and state-of-the art transmission electron microscopy and real-time...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Humana Press
[2021]
|
| Schriftenreihe: | Methods in molecular biology
2555 |
| Schlagworte: | |
| Zusammenfassung: | This volume provides detailed protocols for the performance, analysis, and troubleshooting of in vitro and in vivo experiments related to programmed cell death. Chapters compile conventional techniques such as western blot and qPCR and state-of-the art transmission electron microscopy and real-time multiplexed imaging assays. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Detection of Cell Death Mechanisms: Methods and Protocols aims to ensure seamless execution of protocols on specific cell death type |
| Beschreibung: | This volume provides detailed protocols for the performance, analysis, and troubleshooting of in vitro and in vivo experiments related to programmed cell death. Chapters compile conventional techniques such as western blot and qPCR and state-of-the art transmission electron microscopy and real-time multiplexed imaging assays. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.Authoritative and accessible, Detection of Cell Death Mechanisms: Methods and Protocols aims to ensure seamless execution of protocols on specific cell death type 1. Determination of Caspase Activation by Western Blot; Hussein Chehade, Alexandra Fox, Gil Mor, and Ayesha B. Alvero; ; 2. Detection of Unfolded Protein Response by Polymerase Chain Reaction; Alexander Paridon, Alexandra Fox, and Ayesha B. Alvero; ; 3. Subcellular Fractionation to Demonstrate Activation of Intrinsic Apoptotic Pathway; Hussein Chehade, Alexandra Fox, Gil Mor, and Ayesha B. Alvero; ; 4. A Triple-Parameter Based Laboratory Friendly Fluorescence Imaging to Identify Apoptosis in Live Cells; Pradip De, Jennifer Carlson Aske, and Nandini Dey; ; 5. Flow Cytometric Analyses of p53-mediated Cell Cycle Arrest and Apoptosis in Cancer Cells; Nour N. Al Zouabi, Cai M. Roberts, Z. Ping Lin, and Elena S. Ratner; ; 6. A Real-Time, Bioluminescent Apoptosis Assay; Kevin R. Kupcho and Andrew L. Niles; ; 7. Detection of Anoikis Using Cell Viability Dye and Quantitation of Caspase Activity ; Roslyn Tedja, Alexandra Fox, and Ayesha B. Alvero; ; 8. - Time and Dose Dependent Toxicity Studies in 3D Cultures Using a Luminescent Lactate Dehydrogenase Assay ; Natasha Karassina, Peter Hofsteen, James Cali, and Jolanta Vidugiriene; ; 9. Visualization and Quantification of Neutrophil Extracellular Traps; Mancy Tong and Vikki M Abrahams; ; 10. 2In Vitro Identification and Isolation of Human Neutrophil Extracellular Traps; Guillermina Calo, Analia Silvina Trevani, Esteban Grasso, Irene Angelica Keitelman, Rosanna Ramhorst, Claudia Pérez Leirós, and Florencia Sabbione; ; 11. Induction and Detection of Necroptotic Cell Death in Mammalian Cell Culture; Mikhail Chesnokov, Imran Khan, and Ilana Chefetz; ; 12. Visualization of Necroptotic Cell Death through Transmission Electron Microscopy; Naresh Golla, Linda J. Hong, and Ilana Chefetz; ; 13. Methodology for Comprehensive Detection of Pyroptosis; Yang Feng and Xiaoli Huang; ; 14. Determination and Quantitation of Cytotoxic T Cell-Mediated Cell Death; Han-Hsuan Fu and Harry Qui; ; 15. - Detection of Immunogenic Cell Death in Tumor Vaccination Mouse Model; Kazuki Tatsuno, Patrick Han, Richard Edelson, and Douglas Hanlon; ; 16. A Luminescent Assay to Quantify Cell Viability in Real Time ; Peter Hofsteen, Natasha Karassina, James J. Cali, and Jolanta Vidugiriene; ; 17. Kinetic Measurement of Apoptosis and Immune Cell Killing Using Live-Cell Imaging and Analysis; Jill E. Granger and Daniel M. Appledorn; ; 18. Two Plasmid-Based Systems for CRISPR/Cas9 Mediated Knockout of Target Genes; Cai M Roberts and Elena S Ratner; ; 19. Determining Cell Death Pathway and Regulation by Enrichment Analysis; Katherine Gurdziel ; ; 20. Transcription Factor Binding Site Identification and Enrichment Analysis; Joe L. Guy and Gil Mor |
| Beschreibung: | x, 265 Seiten Illustrationen, Diagramme 529 grams |
| ISBN: | 9781071611647 |
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| 500 | |a 1. Determination of Caspase Activation by Western Blot; Hussein Chehade, Alexandra Fox, Gil Mor, and Ayesha B. Alvero; ; 2. Detection of Unfolded Protein Response by Polymerase Chain Reaction; Alexander Paridon, Alexandra Fox, and Ayesha B. Alvero; ; 3. Subcellular Fractionation to Demonstrate Activation of Intrinsic Apoptotic Pathway; Hussein Chehade, Alexandra Fox, Gil Mor, and Ayesha B. Alvero; ; 4. A Triple-Parameter Based Laboratory Friendly Fluorescence Imaging to Identify Apoptosis in Live Cells; Pradip De, Jennifer Carlson Aske, and Nandini Dey; ; 5. Flow Cytometric Analyses of p53-mediated Cell Cycle Arrest and Apoptosis in Cancer Cells; Nour N. Al Zouabi, Cai M. Roberts, Z. Ping Lin, and Elena S. Ratner; ; 6. A Real-Time, Bioluminescent Apoptosis Assay; Kevin R. Kupcho and Andrew L. Niles; ; 7. Detection of Anoikis Using Cell Viability Dye and Quantitation of Caspase Activity ; Roslyn Tedja, Alexandra Fox, and Ayesha B. Alvero; ; 8. | ||
| 500 | |a - Time and Dose Dependent Toxicity Studies in 3D Cultures Using a Luminescent Lactate Dehydrogenase Assay ; Natasha Karassina, Peter Hofsteen, James Cali, and Jolanta Vidugiriene; ; 9. Visualization and Quantification of Neutrophil Extracellular Traps; Mancy Tong and Vikki M Abrahams; ; 10. 2In Vitro Identification and Isolation of Human Neutrophil Extracellular Traps; Guillermina Calo, Analia Silvina Trevani, Esteban Grasso, Irene Angelica Keitelman, Rosanna Ramhorst, Claudia Pérez Leirós, and Florencia Sabbione; ; 11. Induction and Detection of Necroptotic Cell Death in Mammalian Cell Culture; Mikhail Chesnokov, Imran Khan, and Ilana Chefetz; ; 12. Visualization of Necroptotic Cell Death through Transmission Electron Microscopy; Naresh Golla, Linda J. Hong, and Ilana Chefetz; ; 13. Methodology for Comprehensive Detection of Pyroptosis; Yang Feng and Xiaoli Huang; ; 14. Determination and Quantitation of Cytotoxic T Cell-Mediated Cell Death; Han-Hsuan Fu and Harry Qui; ; 15. | ||
| 500 | |a - Detection of Immunogenic Cell Death in Tumor Vaccination Mouse Model; Kazuki Tatsuno, Patrick Han, Richard Edelson, and Douglas Hanlon; ; 16. A Luminescent Assay to Quantify Cell Viability in Real Time ; Peter Hofsteen, Natasha Karassina, James J. Cali, and Jolanta Vidugiriene; ; 17. Kinetic Measurement of Apoptosis and Immune Cell Killing Using Live-Cell Imaging and Analysis; Jill E. Granger and Daniel M. Appledorn; ; 18. Two Plasmid-Based Systems for CRISPR/Cas9 Mediated Knockout of Target Genes; Cai M Roberts and Elena S Ratner; ; 19. Determining Cell Death Pathway and Regulation by Enrichment Analysis; Katherine Gurdziel ; ; 20. Transcription Factor Binding Site Identification and Enrichment Analysis; Joe L. Guy and Gil Mor | ||
| 520 | |a This volume provides detailed protocols for the performance, analysis, and troubleshooting of in vitro and in vivo experiments related to programmed cell death. Chapters compile conventional techniques such as western blot and qPCR and state-of-the art transmission electron microscopy and real-time multiplexed imaging assays. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Detection of Cell Death Mechanisms: Methods and Protocols aims to ensure seamless execution of protocols on specific cell death type | ||
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| illustrated | Illustrated |
| index_date | 2024-07-03T18:52:15Z |
| indexdate | 2024-07-10T09:18:33Z |
| institution | BVB |
| isbn | 9781071611647 |
| language | English |
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| series | Methods in molecular biology |
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| spelling | Detection of cell death mechanisms methods and protocols edited by Ayesha B. Alvero and Gil G. Mor New York, NY Humana Press [2021] x, 265 Seiten Illustrationen, Diagramme 529 grams txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 2555 This volume provides detailed protocols for the performance, analysis, and troubleshooting of in vitro and in vivo experiments related to programmed cell death. Chapters compile conventional techniques such as western blot and qPCR and state-of-the art transmission electron microscopy and real-time multiplexed imaging assays. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.Authoritative and accessible, Detection of Cell Death Mechanisms: Methods and Protocols aims to ensure seamless execution of protocols on specific cell death type 1. Determination of Caspase Activation by Western Blot; Hussein Chehade, Alexandra Fox, Gil Mor, and Ayesha B. Alvero; ; 2. Detection of Unfolded Protein Response by Polymerase Chain Reaction; Alexander Paridon, Alexandra Fox, and Ayesha B. Alvero; ; 3. Subcellular Fractionation to Demonstrate Activation of Intrinsic Apoptotic Pathway; Hussein Chehade, Alexandra Fox, Gil Mor, and Ayesha B. Alvero; ; 4. A Triple-Parameter Based Laboratory Friendly Fluorescence Imaging to Identify Apoptosis in Live Cells; Pradip De, Jennifer Carlson Aske, and Nandini Dey; ; 5. Flow Cytometric Analyses of p53-mediated Cell Cycle Arrest and Apoptosis in Cancer Cells; Nour N. Al Zouabi, Cai M. Roberts, Z. Ping Lin, and Elena S. Ratner; ; 6. A Real-Time, Bioluminescent Apoptosis Assay; Kevin R. Kupcho and Andrew L. Niles; ; 7. Detection of Anoikis Using Cell Viability Dye and Quantitation of Caspase Activity ; Roslyn Tedja, Alexandra Fox, and Ayesha B. Alvero; ; 8. - Time and Dose Dependent Toxicity Studies in 3D Cultures Using a Luminescent Lactate Dehydrogenase Assay ; Natasha Karassina, Peter Hofsteen, James Cali, and Jolanta Vidugiriene; ; 9. Visualization and Quantification of Neutrophil Extracellular Traps; Mancy Tong and Vikki M Abrahams; ; 10. 2In Vitro Identification and Isolation of Human Neutrophil Extracellular Traps; Guillermina Calo, Analia Silvina Trevani, Esteban Grasso, Irene Angelica Keitelman, Rosanna Ramhorst, Claudia Pérez Leirós, and Florencia Sabbione; ; 11. Induction and Detection of Necroptotic Cell Death in Mammalian Cell Culture; Mikhail Chesnokov, Imran Khan, and Ilana Chefetz; ; 12. Visualization of Necroptotic Cell Death through Transmission Electron Microscopy; Naresh Golla, Linda J. Hong, and Ilana Chefetz; ; 13. Methodology for Comprehensive Detection of Pyroptosis; Yang Feng and Xiaoli Huang; ; 14. Determination and Quantitation of Cytotoxic T Cell-Mediated Cell Death; Han-Hsuan Fu and Harry Qui; ; 15. - Detection of Immunogenic Cell Death in Tumor Vaccination Mouse Model; Kazuki Tatsuno, Patrick Han, Richard Edelson, and Douglas Hanlon; ; 16. A Luminescent Assay to Quantify Cell Viability in Real Time ; Peter Hofsteen, Natasha Karassina, James J. Cali, and Jolanta Vidugiriene; ; 17. Kinetic Measurement of Apoptosis and Immune Cell Killing Using Live-Cell Imaging and Analysis; Jill E. Granger and Daniel M. Appledorn; ; 18. Two Plasmid-Based Systems for CRISPR/Cas9 Mediated Knockout of Target Genes; Cai M Roberts and Elena S Ratner; ; 19. Determining Cell Death Pathway and Regulation by Enrichment Analysis; Katherine Gurdziel ; ; 20. Transcription Factor Binding Site Identification and Enrichment Analysis; Joe L. Guy and Gil Mor This volume provides detailed protocols for the performance, analysis, and troubleshooting of in vitro and in vivo experiments related to programmed cell death. Chapters compile conventional techniques such as western blot and qPCR and state-of-the art transmission electron microscopy and real-time multiplexed imaging assays. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Detection of Cell Death Mechanisms: Methods and Protocols aims to ensure seamless execution of protocols on specific cell death type Apoptosis Life sciences Molekularbiologische Methode (DE-588)7650063-9 gnd rswk-swf Apoptosis (DE-588)4291096-1 gnd rswk-swf Hardcover, Softcover / Biologie/Mikrobiologie Apoptosis (DE-588)4291096-1 s Molekularbiologische Methode (DE-588)7650063-9 s DE-604 Alvero, Ayesha B. edt Mor, Gil Sonstige (DE-588)173847536 oth Erscheint auch als Online-Ausgabe 978-1-07-161162-3 Methods in molecular biology 2555 (DE-604)BV035362695 2255 |
| spellingShingle | Detection of cell death mechanisms methods and protocols Methods in molecular biology Apoptosis Life sciences Molekularbiologische Methode (DE-588)7650063-9 gnd Apoptosis (DE-588)4291096-1 gnd |
| subject_GND | (DE-588)7650063-9 (DE-588)4291096-1 |
| title | Detection of cell death mechanisms methods and protocols |
| title_auth | Detection of cell death mechanisms methods and protocols |
| title_exact_search | Detection of cell death mechanisms methods and protocols |
| title_exact_search_txtP | Detection of cell death mechanisms methods and protocols |
| title_full | Detection of cell death mechanisms methods and protocols edited by Ayesha B. Alvero and Gil G. Mor |
| title_fullStr | Detection of cell death mechanisms methods and protocols edited by Ayesha B. Alvero and Gil G. Mor |
| title_full_unstemmed | Detection of cell death mechanisms methods and protocols edited by Ayesha B. Alvero and Gil G. Mor |
| title_short | Detection of cell death mechanisms |
| title_sort | detection of cell death mechanisms methods and protocols |
| title_sub | methods and protocols |
| topic | Apoptosis Life sciences Molekularbiologische Methode (DE-588)7650063-9 gnd Apoptosis (DE-588)4291096-1 gnd |
| topic_facet | Apoptosis Life sciences Molekularbiologische Methode |
| volume_link | (DE-604)BV035362695 |
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