Germline development in the zebrafish: methods and protocols
This volume details a wide range of methods, ranging from beginner through advanced, used tofurther study zebrafish and fish germline. Chapter guide readers through cultivating and manipulating germ cells, imaging of germline processes and the molecular analysis of their, protein, and RNA. Written i...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Humana Press
[2021]
|
| Schriftenreihe: | Methods in molecular biology
2218 |
| Schlagworte: | |
| Zusammenfassung: | This volume details a wide range of methods, ranging from beginner through advanced, used tofurther study zebrafish and fish germline. Chapter guide readers through cultivating and manipulating germ cells, imaging of germline processes and the molecular analysis of their, protein, and RNA. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Germline Development in the Zebrafish: Methods and Protocols aims to ensure successful results in the further study of this vital field |
| Beschreibung: | 1. Separation of Oocyte and Follicle Layer for Gene Expression Analysis in Zebrafish; Nana Ai, Lin Liu, Esther Shuk-Wa Lau, Anna Chung-Kwan Tse, and Wei Ge; ; 2. The Xenopus Oocyte as an Expression System for Functional Analyses of Fish Aquaporins; François Chauvigné, Alba Ferré, and Joan Cerdà; ; 3. Computer-Assisted Sperm Analysis to Test Environmental Toxicants; Tímea Kollár, Ákos Horváth, and Zsolt Csenki-Bakos; ; 4. Cryopreservation and Transplantation of Spermatogonial Stem Cells; Zoran Marinovic, Jelena Lujic, Qian Li, Yoshiko Iwasaki, Béla Urbányi, Goro Yoshizaki, and Ákos Horváth; ; 5. Masculinization of Zebrafish through Partial Depletion of Primordial Germ Cells by Injecting Diluted Morpholino Oligonucleotides into Embryos; László Orbán, Jolly M. Saju, Keh-Weei Tzung, and Woei Chang Liew; ; 6. Chemical Genetics: Manipulating the Germline with Small Molecules; Youngnam N. Jin and Randall T. Peterson; ; 7. - In vitro Induction of Teleost PGCs; Vanesa Robles, David G. Valcarce, and Marta F. Riesco; ; 8. Applying Rho Pathway Inhibitors to Investigate Germ Plasm Localization; Jerónimo Miranda and Denhí Schnabel; ; 9. Cryopreservation of Pooled Sperm Samples; Jennifer L. Matthews and Zoltan M. Varga; ; 10. Quantifying Tissue Tension in the Granulosa Layer after Laser Surgery; Peng Xia and Carl-Philipp Heisenberg; ; 11. In-vivo and Ex-vivo CT Imaging of Zebrafish Gonads; Christian Dullin and Louisa Habich; ; 12. Live and Time-lapse Imaging of Early Oogenesis and Meiotic Chromosomal Dynamics in Cultured Juvenile Zebrafish Ovaries; Avishag Mytlis and Yaniv M. Elkouby; ; 13. Detection of the Polar Body after Fertilization; Hua Ruan, Xiaogui Yi and Honghui Huang; ; 14. Labelling the Micropylar Cell in Zebrafish Whole-mount and Cryo-sectioned Follicles; Chaitanya Dingare, Petra A. Klemmt, Alina Niedzwetzki,Virginie Lecaudey; ; 15. - Visualisation of Transcriptional Activity in Early Zebrafish Primordial Germ Cells ; Fabio M. D’Orazio, Aleksandra Jasiulewicz, Yavor Hadzhiev, and Ferenc Müller; ; 16. Experimental Methodologies to Visualise Early Cell Biology in Zebrafish Embryos; Asfa Sabrin Borbor and Sreelaja Nair ; ; 17. Observation of Medaka Larval Gonads by Immunohistochemistry and Confocal Laser Microscopy ; Toshiya Nishimura and Minoru Tanaka; ; 18. Methods for Visualization of RNA and Cytoskeletal Elements In The Early Zebrafish Embryo; Hansen, C.L. and Pelegri, F.; ; 19. Glyoxal Fixation as an Alternative for Zebrafish Embryo Immunostaining; Nadia Rostam and Roland Dosch; ; 20. Histological Analysis of Gonads in Zebrafish; Kellee R. Siegfried and Jocelyn S. Steinfeld; ; 21. Manipulating and Visualizing the Germline with Transgenic Lines; Ding Ye and Yonghua Sun; ; 22. - Liquid Chromatography and Tandem Mass Spectrometry in Label Free Protein Quantification of Zebrafish (Danio rerio) Eggs; Ozlem Yilmaz, Emmanuelle Com, Regis Lavigne, Charles Pineau, and Julien Bobe; ; 23. In vivo Protein Lifetime Measurements across Multiple Organs in the Zebrafish; Sunit Mandad, Gudrun Kracht, and Eugenio F. Fornasiero; ; 24. In vivo Imaging of Protein Interactions in the Germ Plasm with Bimolecular Fluorescent Complementation; Roshan Perera and Roland Dosch; ; 25. The Zygotic Genome Activation: Critical Prelude to the Most Important Time of Your Life; Vladimir Korzh; ; 26. Identification of RNA-binding Protein Landscapes Across Zebrafish Embryonic Transcriptome via iCLIP Approach; Vladimir Despic; ; 27. Tethered Function Assay to Study RNA Regulatory Proteins in Zebrafish Embryos; Yuichiro Mishima and Kunio Inoue; ; 28. Massively Parallel Analysis of Regulatory RNA Sequences; Michal Rabani; ; 29. - Exploring Translational Control of Maternal mRNAs in Zebrafish; Cecilia Lanny Winata, Maciej Lapinski, Hisyam Ismail, Sinnakaruppan Mathavan, and Prabha Sampath; |
| Beschreibung: | xiv, 383 Seiten Illustrationen 947 grams |
| ISBN: | 9781071609699 |
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| 490 | 1 | |a Methods in molecular biology |v 2218 | |
| 500 | |a 1. Separation of Oocyte and Follicle Layer for Gene Expression Analysis in Zebrafish; Nana Ai, Lin Liu, Esther Shuk-Wa Lau, Anna Chung-Kwan Tse, and Wei Ge; ; 2. The Xenopus Oocyte as an Expression System for Functional Analyses of Fish Aquaporins; François Chauvigné, Alba Ferré, and Joan Cerdà; ; 3. Computer-Assisted Sperm Analysis to Test Environmental Toxicants; Tímea Kollár, Ákos Horváth, and Zsolt Csenki-Bakos; ; 4. Cryopreservation and Transplantation of Spermatogonial Stem Cells; Zoran Marinovic, Jelena Lujic, Qian Li, Yoshiko Iwasaki, Béla Urbányi, Goro Yoshizaki, and Ákos Horváth; ; 5. Masculinization of Zebrafish through Partial Depletion of Primordial Germ Cells by Injecting Diluted Morpholino Oligonucleotides into Embryos; László Orbán, Jolly M. Saju, Keh-Weei Tzung, and Woei Chang Liew; ; 6. Chemical Genetics: Manipulating the Germline with Small Molecules; Youngnam N. Jin and Randall T. Peterson; ; 7. | ||
| 500 | |a - In vitro Induction of Teleost PGCs; Vanesa Robles, David G. Valcarce, and Marta F. Riesco; ; 8. Applying Rho Pathway Inhibitors to Investigate Germ Plasm Localization; Jerónimo Miranda and Denhí Schnabel; ; 9. Cryopreservation of Pooled Sperm Samples; Jennifer L. Matthews and Zoltan M. Varga; ; 10. Quantifying Tissue Tension in the Granulosa Layer after Laser Surgery; Peng Xia and Carl-Philipp Heisenberg; ; 11. In-vivo and Ex-vivo CT Imaging of Zebrafish Gonads; Christian Dullin and Louisa Habich; ; 12. Live and Time-lapse Imaging of Early Oogenesis and Meiotic Chromosomal Dynamics in Cultured Juvenile Zebrafish Ovaries; Avishag Mytlis and Yaniv M. Elkouby; ; 13. Detection of the Polar Body after Fertilization; Hua Ruan, Xiaogui Yi and Honghui Huang; ; 14. Labelling the Micropylar Cell in Zebrafish Whole-mount and Cryo-sectioned Follicles; Chaitanya Dingare, Petra A. Klemmt, Alina Niedzwetzki,Virginie Lecaudey; ; 15. | ||
| 500 | |a - Visualisation of Transcriptional Activity in Early Zebrafish Primordial Germ Cells ; Fabio M. D’Orazio, Aleksandra Jasiulewicz, Yavor Hadzhiev, and Ferenc Müller; ; 16. Experimental Methodologies to Visualise Early Cell Biology in Zebrafish Embryos; Asfa Sabrin Borbor and Sreelaja Nair ; ; 17. Observation of Medaka Larval Gonads by Immunohistochemistry and Confocal Laser Microscopy ; Toshiya Nishimura and Minoru Tanaka; ; 18. Methods for Visualization of RNA and Cytoskeletal Elements In The Early Zebrafish Embryo; Hansen, C.L. and Pelegri, F.; ; 19. Glyoxal Fixation as an Alternative for Zebrafish Embryo Immunostaining; Nadia Rostam and Roland Dosch; ; 20. Histological Analysis of Gonads in Zebrafish; Kellee R. Siegfried and Jocelyn S. Steinfeld; ; 21. Manipulating and Visualizing the Germline with Transgenic Lines; Ding Ye and Yonghua Sun; ; 22. | ||
| 500 | |a - Liquid Chromatography and Tandem Mass Spectrometry in Label Free Protein Quantification of Zebrafish (Danio rerio) Eggs; Ozlem Yilmaz, Emmanuelle Com, Regis Lavigne, Charles Pineau, and Julien Bobe; ; 23. In vivo Protein Lifetime Measurements across Multiple Organs in the Zebrafish; Sunit Mandad, Gudrun Kracht, and Eugenio F. Fornasiero; ; 24. In vivo Imaging of Protein Interactions in the Germ Plasm with Bimolecular Fluorescent Complementation; Roshan Perera and Roland Dosch; ; 25. The Zygotic Genome Activation: Critical Prelude to the Most Important Time of Your Life; Vladimir Korzh; ; 26. Identification of RNA-binding Protein Landscapes Across Zebrafish Embryonic Transcriptome via iCLIP Approach; Vladimir Despic; ; 27. Tethered Function Assay to Study RNA Regulatory Proteins in Zebrafish Embryos; Yuichiro Mishima and Kunio Inoue; ; 28. Massively Parallel Analysis of Regulatory RNA Sequences; Michal Rabani; ; 29. | ||
| 500 | |a - Exploring Translational Control of Maternal mRNAs in Zebrafish; Cecilia Lanny Winata, Maciej Lapinski, Hisyam Ismail, Sinnakaruppan Mathavan, and Prabha Sampath; | ||
| 520 | |a This volume details a wide range of methods, ranging from beginner through advanced, used tofurther study zebrafish and fish germline. Chapter guide readers through cultivating and manipulating germ cells, imaging of germline processes and the molecular analysis of their, protein, and RNA. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Germline Development in the Zebrafish: Methods and Protocols aims to ensure successful results in the further study of this vital field | ||
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| id | DE-604.BV047659968 |
| illustrated | Illustrated |
| index_date | 2024-07-03T18:52:15Z |
| indexdate | 2024-07-10T09:18:33Z |
| institution | BVB |
| isbn | 9781071609699 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-033044807 |
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| owner_facet | DE-29T |
| physical | xiv, 383 Seiten Illustrationen 947 grams |
| publishDate | 2021 |
| publishDateSearch | 2021 |
| publishDateSort | 2021 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Germline development in the zebrafish methods and protocols edited by Roland Dosch New York, NY Humana Press [2021] xiv, 383 Seiten Illustrationen 947 grams txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 2218 1. Separation of Oocyte and Follicle Layer for Gene Expression Analysis in Zebrafish; Nana Ai, Lin Liu, Esther Shuk-Wa Lau, Anna Chung-Kwan Tse, and Wei Ge; ; 2. The Xenopus Oocyte as an Expression System for Functional Analyses of Fish Aquaporins; François Chauvigné, Alba Ferré, and Joan Cerdà; ; 3. Computer-Assisted Sperm Analysis to Test Environmental Toxicants; Tímea Kollár, Ákos Horváth, and Zsolt Csenki-Bakos; ; 4. Cryopreservation and Transplantation of Spermatogonial Stem Cells; Zoran Marinovic, Jelena Lujic, Qian Li, Yoshiko Iwasaki, Béla Urbányi, Goro Yoshizaki, and Ákos Horváth; ; 5. Masculinization of Zebrafish through Partial Depletion of Primordial Germ Cells by Injecting Diluted Morpholino Oligonucleotides into Embryos; László Orbán, Jolly M. Saju, Keh-Weei Tzung, and Woei Chang Liew; ; 6. Chemical Genetics: Manipulating the Germline with Small Molecules; Youngnam N. Jin and Randall T. Peterson; ; 7. - In vitro Induction of Teleost PGCs; Vanesa Robles, David G. Valcarce, and Marta F. Riesco; ; 8. Applying Rho Pathway Inhibitors to Investigate Germ Plasm Localization; Jerónimo Miranda and Denhí Schnabel; ; 9. Cryopreservation of Pooled Sperm Samples; Jennifer L. Matthews and Zoltan M. Varga; ; 10. Quantifying Tissue Tension in the Granulosa Layer after Laser Surgery; Peng Xia and Carl-Philipp Heisenberg; ; 11. In-vivo and Ex-vivo CT Imaging of Zebrafish Gonads; Christian Dullin and Louisa Habich; ; 12. Live and Time-lapse Imaging of Early Oogenesis and Meiotic Chromosomal Dynamics in Cultured Juvenile Zebrafish Ovaries; Avishag Mytlis and Yaniv M. Elkouby; ; 13. Detection of the Polar Body after Fertilization; Hua Ruan, Xiaogui Yi and Honghui Huang; ; 14. Labelling the Micropylar Cell in Zebrafish Whole-mount and Cryo-sectioned Follicles; Chaitanya Dingare, Petra A. Klemmt, Alina Niedzwetzki,Virginie Lecaudey; ; 15. - Visualisation of Transcriptional Activity in Early Zebrafish Primordial Germ Cells ; Fabio M. D’Orazio, Aleksandra Jasiulewicz, Yavor Hadzhiev, and Ferenc Müller; ; 16. Experimental Methodologies to Visualise Early Cell Biology in Zebrafish Embryos; Asfa Sabrin Borbor and Sreelaja Nair ; ; 17. Observation of Medaka Larval Gonads by Immunohistochemistry and Confocal Laser Microscopy ; Toshiya Nishimura and Minoru Tanaka; ; 18. Methods for Visualization of RNA and Cytoskeletal Elements In The Early Zebrafish Embryo; Hansen, C.L. and Pelegri, F.; ; 19. Glyoxal Fixation as an Alternative for Zebrafish Embryo Immunostaining; Nadia Rostam and Roland Dosch; ; 20. Histological Analysis of Gonads in Zebrafish; Kellee R. Siegfried and Jocelyn S. Steinfeld; ; 21. Manipulating and Visualizing the Germline with Transgenic Lines; Ding Ye and Yonghua Sun; ; 22. - Liquid Chromatography and Tandem Mass Spectrometry in Label Free Protein Quantification of Zebrafish (Danio rerio) Eggs; Ozlem Yilmaz, Emmanuelle Com, Regis Lavigne, Charles Pineau, and Julien Bobe; ; 23. In vivo Protein Lifetime Measurements across Multiple Organs in the Zebrafish; Sunit Mandad, Gudrun Kracht, and Eugenio F. Fornasiero; ; 24. In vivo Imaging of Protein Interactions in the Germ Plasm with Bimolecular Fluorescent Complementation; Roshan Perera and Roland Dosch; ; 25. The Zygotic Genome Activation: Critical Prelude to the Most Important Time of Your Life; Vladimir Korzh; ; 26. Identification of RNA-binding Protein Landscapes Across Zebrafish Embryonic Transcriptome via iCLIP Approach; Vladimir Despic; ; 27. Tethered Function Assay to Study RNA Regulatory Proteins in Zebrafish Embryos; Yuichiro Mishima and Kunio Inoue; ; 28. Massively Parallel Analysis of Regulatory RNA Sequences; Michal Rabani; ; 29. - Exploring Translational Control of Maternal mRNAs in Zebrafish; Cecilia Lanny Winata, Maciej Lapinski, Hisyam Ismail, Sinnakaruppan Mathavan, and Prabha Sampath; This volume details a wide range of methods, ranging from beginner through advanced, used tofurther study zebrafish and fish germline. Chapter guide readers through cultivating and manipulating germ cells, imaging of germline processes and the molecular analysis of their, protein, and RNA. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Germline Development in the Zebrafish: Methods and Protocols aims to ensure successful results in the further study of this vital field Cell biology Life sciences Zebrabärbling (DE-588)4079468-4 gnd rswk-swf Molekularbiologische Methode (DE-588)7650063-9 gnd rswk-swf Keimbahn (DE-588)4030158-8 gnd rswk-swf Hardcover, Softcover / Biologie/Mikrobiologie Zebrabärbling (DE-588)4079468-4 s Keimbahn (DE-588)4030158-8 s Molekularbiologische Methode (DE-588)7650063-9 s DE-604 Dosch, Roland (DE-588)123182218X edt Erscheint auch als Online-Ausgabe 978-1-0716-0970-5 Methods in molecular biology 2218 (DE-604)BV035362695 2218 |
| spellingShingle | Germline development in the zebrafish methods and protocols Methods in molecular biology Cell biology Life sciences Zebrabärbling (DE-588)4079468-4 gnd Molekularbiologische Methode (DE-588)7650063-9 gnd Keimbahn (DE-588)4030158-8 gnd |
| subject_GND | (DE-588)4079468-4 (DE-588)7650063-9 (DE-588)4030158-8 |
| title | Germline development in the zebrafish methods and protocols |
| title_auth | Germline development in the zebrafish methods and protocols |
| title_exact_search | Germline development in the zebrafish methods and protocols |
| title_exact_search_txtP | Germline development in the zebrafish methods and protocols |
| title_full | Germline development in the zebrafish methods and protocols edited by Roland Dosch |
| title_fullStr | Germline development in the zebrafish methods and protocols edited by Roland Dosch |
| title_full_unstemmed | Germline development in the zebrafish methods and protocols edited by Roland Dosch |
| title_short | Germline development in the zebrafish |
| title_sort | germline development in the zebrafish methods and protocols |
| title_sub | methods and protocols |
| topic | Cell biology Life sciences Zebrabärbling (DE-588)4079468-4 gnd Molekularbiologische Methode (DE-588)7650063-9 gnd Keimbahn (DE-588)4030158-8 gnd |
| topic_facet | Cell biology Life sciences Zebrabärbling Molekularbiologische Methode Keimbahn |
| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT doschroland germlinedevelopmentinthezebrafishmethodsandprotocols |