Protein acetylation methods and protocols - Brosh, Robert M. (Springer New York, 2019)
High-Resolution Mass Spectrometry to Identify and Quantify Acetylation Protein Targets -- Isolation of Proteins on Nascent Chromatin and Characterization by Quantitative Mass Spectrometry -- Detection and Quantitation of Acetylated Histones on Replicating DNA using In Situ Proximity Ligation Assay and Click-it Chemisty -- Quantification of In Vitro Protein Lysine Acetylation by Reversed Phase HPLC -- Measurement and Analysis of Lysine Acetylation by Kat Complexes In Vitro and In Vivo -- Site-Specific Lysine Acetylation Stoichiometry across Subcellular Compartments -- Lysine Acetylation of Proteins and its Characterization in Human Systems -- Molecular and Cellular Characterization of SIRT1 Allosteric Activators -- Assessment of SIRT2 Inhibitors in Mouse Models of Cancer -- Using Yeast as a Model Organism to Study the Functional Roles of Histone Acetylation in DNA Excision Repair -- Biochemical and Cellular Assays to Assess the Effects of Acetylation on Base Excision Repair Enzymes -- Analysis of DNA Processing Enzyme FEN1 and its Regulation by Protein Lysine Acetylation -- Examining the Role of HDACs in DNA Double-Strand Break Repair in Neurons -- Experimental Approaches to Investigate the Role of Helicase Acetylation in Regulating R-Loop Stability -- Rapid Detection of p53 Acetylation Status in Response to Cellular Stress Signaling -- Isolation and Quantification Brain Region-Specific and Cell Subtype-Specific Histone (De)Acetylation in Cognitive Neuroepigenetics -- Functional Analysis of HDACs in Tumorigeneis