Ancient DNA: methods and protocols
This fully updated second edition explores protocols that address the most challenging aspects of experimental work in ancient DNA, such as preparing ancient samples for DNA extraction, the DNA extraction itself, and transforming extracted ancient DNA molecules for sequencing library preparation. Th...
Gespeichert in:
| Weitere Verfasser: | , , , , , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2019
|
| Ausgabe: | 2nd ed. 2019 |
| Schriftenreihe: | Methods in Molecular Biology
1963 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | This fully updated second edition explores protocols that address the most challenging aspects of experimental work in ancient DNA, such as preparing ancient samples for DNA extraction, the DNA extraction itself, and transforming extracted ancient DNA molecules for sequencing library preparation. The volume also examines the analysis of high-throughput sequencing data recovered from ancient specimens, which, because of the degraded nature of ancient DNA and common co-extraction of contaminant DNA, has challenges that are unique compared to data recovered from modern specimens.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Ancient DNA: Methods and Protocols, Second Edition aims to serve both experts and beginners by presenting protocols in a manner that makes them easily accessible for everyday use in the lab |
| Beschreibung: | Setting Up an Ancient DNA Laboratory -- Pretreatment: Removing DNA Contamination from Ancient Bones and Teeth Using Sodium Hypochlorite and Phosphate -- Pretreatment: Improving Endogenous Ancient DNA Yields Using a Simple Enzymatic Pre-Digestion Step -- Extraction of Highly Degraded DNA from Ancient Bones and Teeth -- Sampling and Extraction of Ancient DNA from Sediments -- Extraction of Ancient DNA from Plant Remains -- DNA Extraction from Keratin and Chitin -- Double-Stranded Library Preparation for Ancient and Other Degraded Samples -- A Method for Single-Stranded Ancient DNA Library Preparation -- Sequencing Library Preparation from Degraded Samples for Non-Illumina Sequencing Platforms -- Whole-Genome Capture of Ancient DNA Using Homemade Baits -- Generating RNA Baits for Capture-Based Enrichment -- Hybridization Capture of Ancient DNA Using RNA Baits -- Application of Solid-State Capture for the Retrieval of Small-to-Medium Sized Target Loci from Ancient DNA -- Targeted PCR Amplification and Multiplex Sequencing of Ancient DNA for SNP Analysis -- Targeted Amplification and Sequencing of Ancient Environmental and Sedimentary DNA -- Authentication and Assessment of Contamination in Ancient DNA -- Assembly of Ancient Mitochondrial Genomes without a Closely Related Reference Sequence |
| Beschreibung: | 1 Online-Ressource (X, 216 Seiten) Illustrationen |
| ISBN: | 9781493991761 |
| DOI: | 10.1007/978-1-4939-9176-1 |
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| edition | 2nd ed. 2019 |
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| spelling | Ancient DNA methods and protocols edited by Beth Shapiro, Axel Barlow, Peter D. Heintzman, Michael Hofreiter, Johanna L. A. Paijmans, André E. R. Soares 2nd ed. 2019 New York, NY Springer New York 2019 1 Online-Ressource (X, 216 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1963 Setting Up an Ancient DNA Laboratory -- Pretreatment: Removing DNA Contamination from Ancient Bones and Teeth Using Sodium Hypochlorite and Phosphate -- Pretreatment: Improving Endogenous Ancient DNA Yields Using a Simple Enzymatic Pre-Digestion Step -- Extraction of Highly Degraded DNA from Ancient Bones and Teeth -- Sampling and Extraction of Ancient DNA from Sediments -- Extraction of Ancient DNA from Plant Remains -- DNA Extraction from Keratin and Chitin -- Double-Stranded Library Preparation for Ancient and Other Degraded Samples -- A Method for Single-Stranded Ancient DNA Library Preparation -- Sequencing Library Preparation from Degraded Samples for Non-Illumina Sequencing Platforms -- Whole-Genome Capture of Ancient DNA Using Homemade Baits -- Generating RNA Baits for Capture-Based Enrichment -- Hybridization Capture of Ancient DNA Using RNA Baits -- Application of Solid-State Capture for the Retrieval of Small-to-Medium Sized Target Loci from Ancient DNA -- Targeted PCR Amplification and Multiplex Sequencing of Ancient DNA for SNP Analysis -- Targeted Amplification and Sequencing of Ancient Environmental and Sedimentary DNA -- Authentication and Assessment of Contamination in Ancient DNA -- Assembly of Ancient Mitochondrial Genomes without a Closely Related Reference Sequence This fully updated second edition explores protocols that address the most challenging aspects of experimental work in ancient DNA, such as preparing ancient samples for DNA extraction, the DNA extraction itself, and transforming extracted ancient DNA molecules for sequencing library preparation. The volume also examines the analysis of high-throughput sequencing data recovered from ancient specimens, which, because of the degraded nature of ancient DNA and common co-extraction of contaminant DNA, has challenges that are unique compared to data recovered from modern specimens.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Ancient DNA: Methods and Protocols, Second Edition aims to serve both experts and beginners by presenting protocols in a manner that makes them easily accessible for everyday use in the lab Genetics Evolutionary biology Shapiro, Beth 1976- (DE-588)1075865654 edt Barlow, Axel (DE-588)1161626131 edt Heintzman, Peter D. (DE-588)118301595X edt Hofreiter, Michael 1973- (DE-588)124374433 edt Paijmans, Johanna L. A. (DE-588)1106339428 edt Soares, André E. R. (DE-588)1183021461 edt Erscheint auch als Druck-Ausgabe 978-1-4939-9177-8 https://doi.org/10.1007/978-1-4939-9176-1 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Ancient DNA methods and protocols Genetics Evolutionary biology |
| title | Ancient DNA methods and protocols |
| title_auth | Ancient DNA methods and protocols |
| title_exact_search | Ancient DNA methods and protocols |
| title_exact_search_txtP | Ancient DNA methods and protocols |
| title_full | Ancient DNA methods and protocols edited by Beth Shapiro, Axel Barlow, Peter D. Heintzman, Michael Hofreiter, Johanna L. A. Paijmans, André E. R. Soares |
| title_fullStr | Ancient DNA methods and protocols edited by Beth Shapiro, Axel Barlow, Peter D. Heintzman, Michael Hofreiter, Johanna L. A. Paijmans, André E. R. Soares |
| title_full_unstemmed | Ancient DNA methods and protocols edited by Beth Shapiro, Axel Barlow, Peter D. Heintzman, Michael Hofreiter, Johanna L. A. Paijmans, André E. R. Soares |
| title_short | Ancient DNA |
| title_sort | ancient dna methods and protocols |
| title_sub | methods and protocols |
| topic | Genetics Evolutionary biology |
| topic_facet | Genetics Evolutionary biology |
| url | https://doi.org/10.1007/978-1-4939-9176-1 |
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