Microfluidic electrophoresis: methods and protocols
This detailed book provides a set of protocols necessary for the development of a variety of microchip-based electrophoretic assays. It compiles a range of such electrophoretic methods by leading researchers in the field, covering subjects such as microfluidic device fabrication, on-chip sample prep...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2019
|
| Ausgabe: | 1st ed. 2019 |
| Schriftenreihe: | Methods in Molecular Biology
1906 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | This detailed book provides a set of protocols necessary for the development of a variety of microchip-based electrophoretic assays. It compiles a range of such electrophoretic methods by leading researchers in the field, covering subjects such as microfluidic device fabrication, on-chip sample preparation, theoretical/simulation protocols for assessing these separation methods, as well as common practices followed when applying them to important real world applications. The contents of the book range from protocols for classical assays to those involving pioneering separation techniques recently developed by the scientific community for advancing our analytical capabilities. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Microfluidic Electrophoresis: Methods and Protocols serves as an convenient text for academic researchers as well as practicing engineers, biochemists, and analytical laboratory professionals |
| Beschreibung: | Fabrication of Glass Microfluidic Devices -- Soft Lithography, Molding, and Micromachining Techniques for Polymer Micro Devices -- Sample Injection Techniques -- Sample Preconcentration Protocols in Microfluidic Electrophoresis -- Microchip Electrophoresis Containing Electrodes for Integrated Electrochemical Detection -- Micellar Electrokinetic Chromatography -- Microchip Isotachophoresis: Analysis of Pharmaceuticals -- Microfluidic Free-Flow Isoelectric Focusing with Real-Time pI Determination -- Nanochannel Gradient Separations -- Paper-Based Electrophoresis Microchip as a Powerful Tool for Bioanalytical Applications -- Band Broadening Theories in Capillary Electrophoresis -- Estimating Stream Broadening in Free-Flow Electrophoretic Systems Based on the Method-of-Moments Formulation -- Microchip Electrophoresis Tools for the Analysis of Small Molecules -- Integrated Microfluidic System for Rapid DNA Fingerprint Analysis: A Miniaturized Integrated DNA Analysis System (MiDAS): Swab Sample-In to DNA Profile-Out -- Achieving Stable Electrospray Ionization Mass Spectrometry (ESI-MS) Detection from Microfluidic Chips -- Microchip-Based Electrophoretic Separations with a Pressure-Driven Backflow |
| Beschreibung: | 1 Online-Ressource (X, 255 Seiten) Illustrationen |
| ISBN: | 9781493989645 |
| DOI: | 10.1007/978-1-4939-8964-5 |
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| discipline_str_mv | Chemie / Pharmazie |
| doi_str_mv | 10.1007/978-1-4939-8964-5 |
| edition | 1st ed. 2019 |
| format | Electronic eBook |
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| id | DE-604.BV047645622 |
| illustrated | Not Illustrated |
| index_date | 2024-07-03T18:48:41Z |
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| isbn | 9781493989645 |
| language | English |
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| spelling | Microfluidic electrophoresis methods and protocols edited by Debashis Dutta 1st ed. 2019 New York, NY Springer New York 2019 1 Online-Ressource (X, 255 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1906 Fabrication of Glass Microfluidic Devices -- Soft Lithography, Molding, and Micromachining Techniques for Polymer Micro Devices -- Sample Injection Techniques -- Sample Preconcentration Protocols in Microfluidic Electrophoresis -- Microchip Electrophoresis Containing Electrodes for Integrated Electrochemical Detection -- Micellar Electrokinetic Chromatography -- Microchip Isotachophoresis: Analysis of Pharmaceuticals -- Microfluidic Free-Flow Isoelectric Focusing with Real-Time pI Determination -- Nanochannel Gradient Separations -- Paper-Based Electrophoresis Microchip as a Powerful Tool for Bioanalytical Applications -- Band Broadening Theories in Capillary Electrophoresis -- Estimating Stream Broadening in Free-Flow Electrophoretic Systems Based on the Method-of-Moments Formulation -- Microchip Electrophoresis Tools for the Analysis of Small Molecules -- Integrated Microfluidic System for Rapid DNA Fingerprint Analysis: A Miniaturized Integrated DNA Analysis System (MiDAS): Swab Sample-In to DNA Profile-Out -- Achieving Stable Electrospray Ionization Mass Spectrometry (ESI-MS) Detection from Microfluidic Chips -- Microchip-Based Electrophoretic Separations with a Pressure-Driven Backflow This detailed book provides a set of protocols necessary for the development of a variety of microchip-based electrophoretic assays. It compiles a range of such electrophoretic methods by leading researchers in the field, covering subjects such as microfluidic device fabrication, on-chip sample preparation, theoretical/simulation protocols for assessing these separation methods, as well as common practices followed when applying them to important real world applications. The contents of the book range from protocols for classical assays to those involving pioneering separation techniques recently developed by the scientific community for advancing our analytical capabilities. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Microfluidic Electrophoresis: Methods and Protocols serves as an convenient text for academic researchers as well as practicing engineers, biochemists, and analytical laboratory professionals Electrophoresis Biotechnology Dutta, Debashis (DE-588)1234154188 edt Erscheint auch als Druck-Ausgabe 978-1-4939-8965-2 https://doi.org/10.1007/978-1-4939-8964-5 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Microfluidic electrophoresis methods and protocols Electrophoresis Biotechnology |
| title | Microfluidic electrophoresis methods and protocols |
| title_auth | Microfluidic electrophoresis methods and protocols |
| title_exact_search | Microfluidic electrophoresis methods and protocols |
| title_exact_search_txtP | Microfluidic electrophoresis methods and protocols |
| title_full | Microfluidic electrophoresis methods and protocols edited by Debashis Dutta |
| title_fullStr | Microfluidic electrophoresis methods and protocols edited by Debashis Dutta |
| title_full_unstemmed | Microfluidic electrophoresis methods and protocols edited by Debashis Dutta |
| title_short | Microfluidic electrophoresis |
| title_sort | microfluidic electrophoresis methods and protocols |
| title_sub | methods and protocols |
| topic | Electrophoresis Biotechnology |
| topic_facet | Electrophoresis Biotechnology |
| url | https://doi.org/10.1007/978-1-4939-8964-5 |
| work_keys_str_mv | AT duttadebashis microfluidicelectrophoresismethodsandprotocols |