Stem cells and aging: methods and protocols
This fully updated book collects protocols that reflect current investigations into how stem cell populations change during organismal aging. These methodologies are well-established and described in easy to follow fashion so as to be valuable for not only experts but novices in the ever-developing...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2019
|
| Ausgabe: | 2nd ed. 2019 |
| Schriftenreihe: | Methods in Molecular Biology
2045 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | This fully updated book collects protocols that reflect current investigations into how stem cell populations change during organismal aging. These methodologies are well-established and described in easy to follow fashion so as to be valuable for not only experts but novices in the ever-developing stem cell field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Stem Cells and Aging: Methods and Protocols, Second Edition serves as an ideal guide for all researchers working in this very active area of study |
| Beschreibung: | Assessing Muscle Stem Cell Clonal Complexity during Aging -- Simultaneous Isolation of Stem and Niche Cells of Skeletal Muscle: Applicability for Aging Studies -- Isolation and Culture of Individual Myofibers and Their Adjacent Muscle Stem Cells from Aged and Adult Skeletal Muscle -- Methods and Strategies for Procurement, Isolation, Characterization, and Assessment of Senescence of Human Mesenchymal Stem Cells from Adipose Tissue -- Quantifying Senescence-Associated Phenotypes in Primary Multipotent Mesenchymal Stromal Cell Cultures -- Adipogenic and Osteogenic Differentiation of In Vitro Aged Human Mesenchymal Stem Cells -- Human Skeletal-Muscle-Derived Mesenchymal Stem/Stromal Cell Isolation and Growth Kinetics Analysis -- Complete Assessment of Multilineage Differentiation Potential of Human Skeletal-Muscle-Derived Mesenchymal Stem/Stromal Cells -- Human Synovium-Derived Mesenchymal Stem Cells: Ex Vivo Analysis -- 3D-Embedded Cell Cultures to Study Tendon Biology -- - Targeted, Amplicon-Based, Next-Generation Sequencing to Detect Age-Related Clonal Hematopoiesis -- Column Free Method for Isolation and Culture of C-Kit Positive Stem Cells from Atrial Explants -- Histological Assessment of Cre/loxP Genetic Recombination in the Aging Subventricular Zone of Nestin-CreERT2/Rosa26YFP Mice -- Infrared Spectroscopy and Imagıng in Stem Cells and Agıng Research -- Use of U-STELA for Accurate Measurement of Extremely Short-Telomeres -- Surface-Antigen-Based Identification of In Vitro Expanded Skeletal-Muscle-Derived Mesenchymal Stromal/Stem Cells Using Flow Cytometry -- Analysis of Stem Cells and Their Activity in Human Skeletal Muscles by Immunohistochemistry -- Methods for Detection of Autophagy in Mammalian Cells -- Metabolomic and Proteomic Analyses of Mouse Primordial Germ Cells -- Reprogramming of Aged Cells into Pluripotent Stem Cells by Nuclear Transfer -- - Generation of Retinal Pigmented Epithelial (RPE) Cells for Treatment of Age-Related Macular Degeneration (AMD) -- Histopathological and Behavioral Assessments of Aging Effects on Stem Cell Transplants in an Experimental Traumatic Brain Injury -- 3D Age-Specific Mortality Trajectory: A Survival Analysis Protocol -- Isolation, Expansion, and Characterization of Wharton's Jelly-Derived Mesenchymal Stromal Cell: Method to Identify Functional Passages for Experiments -- CRISPR Base Editing in Induced Pluripotent Stem Cells |
| Beschreibung: | 1 Online-Ressource (XII, 354 Seiten) Illustrationen |
| ISBN: | 9781493997138 |
| DOI: | 10.1007/978-1-4939-9713-8 |
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Datensatz im Suchindex
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| discipline | Biologie |
| discipline_str_mv | Biologie |
| doi_str_mv | 10.1007/978-1-4939-9713-8 |
| edition | 2nd ed. 2019 |
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| illustrated | Not Illustrated |
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| indexdate | 2024-07-10T09:18:10Z |
| institution | BVB |
| isbn | 9781493997138 |
| language | English |
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| spelling | Stem cells and aging methods and protocols edited by Kursad Turksen 2nd ed. 2019 New York, NY Springer New York 2019 1 Online-Ressource (XII, 354 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 2045 Assessing Muscle Stem Cell Clonal Complexity during Aging -- Simultaneous Isolation of Stem and Niche Cells of Skeletal Muscle: Applicability for Aging Studies -- Isolation and Culture of Individual Myofibers and Their Adjacent Muscle Stem Cells from Aged and Adult Skeletal Muscle -- Methods and Strategies for Procurement, Isolation, Characterization, and Assessment of Senescence of Human Mesenchymal Stem Cells from Adipose Tissue -- Quantifying Senescence-Associated Phenotypes in Primary Multipotent Mesenchymal Stromal Cell Cultures -- Adipogenic and Osteogenic Differentiation of In Vitro Aged Human Mesenchymal Stem Cells -- Human Skeletal-Muscle-Derived Mesenchymal Stem/Stromal Cell Isolation and Growth Kinetics Analysis -- Complete Assessment of Multilineage Differentiation Potential of Human Skeletal-Muscle-Derived Mesenchymal Stem/Stromal Cells -- Human Synovium-Derived Mesenchymal Stem Cells: Ex Vivo Analysis -- 3D-Embedded Cell Cultures to Study Tendon Biology -- - Targeted, Amplicon-Based, Next-Generation Sequencing to Detect Age-Related Clonal Hematopoiesis -- Column Free Method for Isolation and Culture of C-Kit Positive Stem Cells from Atrial Explants -- Histological Assessment of Cre/loxP Genetic Recombination in the Aging Subventricular Zone of Nestin-CreERT2/Rosa26YFP Mice -- Infrared Spectroscopy and Imagıng in Stem Cells and Agıng Research -- Use of U-STELA for Accurate Measurement of Extremely Short-Telomeres -- Surface-Antigen-Based Identification of In Vitro Expanded Skeletal-Muscle-Derived Mesenchymal Stromal/Stem Cells Using Flow Cytometry -- Analysis of Stem Cells and Their Activity in Human Skeletal Muscles by Immunohistochemistry -- Methods for Detection of Autophagy in Mammalian Cells -- Metabolomic and Proteomic Analyses of Mouse Primordial Germ Cells -- Reprogramming of Aged Cells into Pluripotent Stem Cells by Nuclear Transfer -- - Generation of Retinal Pigmented Epithelial (RPE) Cells for Treatment of Age-Related Macular Degeneration (AMD) -- Histopathological and Behavioral Assessments of Aging Effects on Stem Cell Transplants in an Experimental Traumatic Brain Injury -- 3D Age-Specific Mortality Trajectory: A Survival Analysis Protocol -- Isolation, Expansion, and Characterization of Wharton's Jelly-Derived Mesenchymal Stromal Cell: Method to Identify Functional Passages for Experiments -- CRISPR Base Editing in Induced Pluripotent Stem Cells This fully updated book collects protocols that reflect current investigations into how stem cell populations change during organismal aging. These methodologies are well-established and described in easy to follow fashion so as to be valuable for not only experts but novices in the ever-developing stem cell field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Stem Cells and Aging: Methods and Protocols, Second Edition serves as an ideal guide for all researchers working in this very active area of study Stem cells Regenerative medicine Tissue engineering Cell physiology Turksen, Kursad ca. 20./21. Jh. (DE-588)1077175922 edt Erscheint auch als Druck-Ausgabe 978-1-4939-9715-2 https://doi.org/10.1007/978-1-4939-9713-8 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Stem cells and aging methods and protocols Stem cells Regenerative medicine Tissue engineering Cell physiology |
| title | Stem cells and aging methods and protocols |
| title_auth | Stem cells and aging methods and protocols |
| title_exact_search | Stem cells and aging methods and protocols |
| title_exact_search_txtP | Stem cells and aging methods and protocols |
| title_full | Stem cells and aging methods and protocols edited by Kursad Turksen |
| title_fullStr | Stem cells and aging methods and protocols edited by Kursad Turksen |
| title_full_unstemmed | Stem cells and aging methods and protocols edited by Kursad Turksen |
| title_short | Stem cells and aging |
| title_sort | stem cells and aging methods and protocols |
| title_sub | methods and protocols |
| topic | Stem cells Regenerative medicine Tissue engineering Cell physiology |
| topic_facet | Stem cells Regenerative medicine Tissue engineering Cell physiology |
| url | https://doi.org/10.1007/978-1-4939-9713-8 |
| work_keys_str_mv | AT turksenkursad stemcellsandagingmethodsandprotocols |