Phloem methods and protocols - Liesche, Johannes 1984- (Springer New York, 2019)
Part 1 : Investigating phloem anatomy -- Optimal preparation of tissue sections for light-microscopic analysis of phloem anatomy -- Transmission electron microscopy of the phloem with minimal artifacts -- Scanning electron microscopy of the phloem -- Non-invasive investigation of phloem structure by 3D synchrotron X-ray microtomography -- Quantification of leaf phloem anatomical features with microscopy -- Methods of phloem visualization - a clear future in sight? -- Part 2 : Visualizing phloem proteins -- Super-resolution microscopy of phloem proteins -- Live-cell imaging of fluorescently-tagged phloem proteins with confocal microscopy -- Immunohistochemical localization of proteins in the phloem: problems and solutions -- Part 3 : Sampling phloem sap -- SStudying phloem loading with EDTA-facilitated phloem exudate collection and analysis -- Measurement of inner bark and leaf osmolality -- Using 13C to quantify phloem transport on tall plants in the field --
- Non-invasive determination of phloem transport speed with carbon-14 (14C) -- Detecting rapid changes in carbon transport and partitioning with carbon-11 (11C) -- In vivo veritas: radiotracers in studies of phloem transport of carbohydrate -- Measuring phloem transport velocity in Arabidopsis seedlings using the fluorescent coumarin glucoside, esculin -- Measuring phloem transport velocity on a tissue-level using a phloem-mobile dye -- Part 4 : Investigating mechanism and quantities of phloem loading and unloading -- Quantification of symplasmic phloem loading capacity with live-cell microscopy -- Assessing Long-distance Carbon Partitioning from Photosynthetic Source Leaves to Heterotrophic Sink Organs with Photoassismilated [14C]CO2 -- Measurement of subcellular metabolite concentrations in relation to phloem loading -- Measuring sucrose transporter activity using a protoplast esculin assay -- The AtSUC2 promoter: A powerful tool to study phloem physiology and development --
- Part 5 : Phloem physiology -- Using aphids to measure turgor pressure inside sieve elements -- Stem compression: A means to reversibly reduce phloem transport in tree stems -- Methods for assessing the role of phloem transport in plant stress responses -- Part 6 : Theoretical models and microfluidics of phloem function -- Modeling the hydraulic conductivity of phloem sieve elements -- Using a multi-compartmental metabolic model to predict carbon allocation in Arabidopsis thaliana -- A mechanistic model to predict distribution of carbon among multiple sinks -- Using a mathematical model of phloem transport to optimize strategies for crop improvement -- Making microfluidic devices that simulate phloem transport -- Part 7 : Signals in the phloem -- Efficient establishment of interfamily heterograft of Nicotiana benthamiana and Arabidopsis thaliana -- Identification of phloem mobile mRNAs using the Solanacea heterograft system --
- Analyzing and predicting phloem mobility of macromolecules with an online database -- Measurement of electropotential waves in intact phloem sieve elements using microelectrodes -- Measurement of electropotential waves in intact sieve elements using aphids as bioelectrodes -- What can cell culture systems reveal about sieve element differentiation?