Streptococcus pneumoniae methods and protocols - Iovino, Federico 1985- (Springer New York, 2019)
Optimal Conditions for Streptococcus pneumoniae Culture: In Solid and Liquid Media -- Electron Microscopy to Study the Fine Structure of the Pneumococcal Cell -- Immunofluorescent Staining and High-Resolution Microscopy to Study the Pneumococcal Cell -- Construction of Fluorescent Pneumococci for In Vivo Imaging and Labeling of the Chromosome -- High-Resolution and Super-Resolution Immunofluorescent Microscopy Ex Vivo to Study Pneumococcal Interactions with the Host -- Natural Genetic Transformation: A Direct Route to Easy Insertion of Chimeric Genes into the Pneumococcal Chromosome -- Gene Expression Analysis in the Pneumococcus -- Transcriptional Knockdown in Pneumococci using CRISPR Interference -- Protein Expression Analysis by Western Blot and Protein-Protein Interactions -- Mass Spectrometry to Study the Bacterial Proteome from a Single Colony -- Bead-Based Flow Cytometric Cell Counting of Live and Dead Bacteria -- In Vitro Adhesion, Invasion, and Transcytosis of Streptococcus pneumonia with Host Cells -- Growing and Characterizing Biofilms Formed by Streptococcus pneumoniae -- In Vivo Mouse Models to Study How Pneumococci Interact with the Host and Cause Invasive Pneumococcal Disease -- Two-Photon Intravital Imaging of Leukocytes in the Trachea during Pneumococcal Infection -- IVIS Spectrum CT to Image the Progression of Pneumococcal Infections In Vivo -- The Pneumococcus and Its Critical Role in Public Health -- The Epidemiology and Biostatistics of Pneumococcus