Lipid-activated nuclear receptors: methods and protocols
This book covers a wide range of state-of-the-art methodologies and detailed protocols currently used to study the actions that lipid-activated nuclear receptors and their co-regulators have in tissues and immune cell types considered classic metabolic "powerhouses". This includes the live...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2019
|
| Ausgabe: | 1st ed. 2019 |
| Schriftenreihe: | Methods in Molecular Biology
1951 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | This book covers a wide range of state-of-the-art methodologies and detailed protocols currently used to study the actions that lipid-activated nuclear receptors and their co-regulators have in tissues and immune cell types considered classic metabolic "powerhouses". This includes the liver, adipose tissue, and monocytes/macrophages present in these and other metabolic tissues. While the main focus is on the oxysterol receptor or Liver X Receptor (LXR), the majority of the methods described can be easily applied to multiple nuclear receptors, as well as to other tissues or cell types. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Lipid-Activated Nuclear Receptors: Methods and Protocols serves as an ideal guide for researchers pursuing the vital study of nuclear receptor biology and beyond |
| Beschreibung: | Quantification of Oxysterol Nuclear Receptor Ligands by LC/MS/MS -- A Stable Luciferase Reporter System to Characterise LXR Regulation by Oxysterols and Novel Ligands -- Isolation and Analysis of Human Monocytes and Adipose Tissue Macrophages -- Glucan Encapsulated siRNA Particles (GeRPs) for Specific Gene Silencing in Adipose Tissue Macrophages -- Isolation and Purification of Tissue Resident Macrophages for the Analysis of Nuclear Receptor Activity -- Bone Marrow-Derived Macrophage Immortalization of LXR Nuclear Receptor-Deficient Cells -- Dual Crosslinking Chromatin Immunoprecipitation Protocol for Next Generation Sequencing (ChIPseq) in Macrophages -- Analysis of LXR Nuclear Receptor Cistrome through ChIP-seq Data Bioinformatics -- Quantifying Cellular Cholesterol Efflux -- Methods for Assessing the Effects of LXR Agonists on Macrophage Bacterial Infection -- Measuring Apoptotic Cell Engulfment (Efferocytosis) Efficiency -- Methods to Study Monocyte and Macrophage Trafficking in Atherosclerosis Progression and Resolution -- Preparation of Frozen Liver Tissues for Integrated Omics Analysis -- Identification of Nuclear Receptor Targets By Chromatin Immunoprecipitation in Fatty Liver -- The LPS/D-Galactosamine-Induced Fulminant Hepatitis Model to Assess the Role of Ligand-Activated Nuclear Receptors on the NLRP3 Inflammasome Pathway In Vivo -- Analyzing T-Cell Plasma Membrane Lipids by Flow Cytometry |
| Beschreibung: | 1 Online-Ressource (XI, 220 Seiten) Illustrationen |
| ISBN: | 9781493991303 |
| DOI: | 10.1007/978-1-4939-9130-3 |
Internformat
MARC
| LEADER | 00000nmm a2200000zc 4500 | ||
|---|---|---|---|
| 001 | BV047639738 | ||
| 003 | DE-604 | ||
| 005 | 00000000000000.0 | ||
| 007 | cr|uuu---uuuuu | ||
| 008 | 211214s2019 |||| o||u| ||||||eng d | ||
| 020 | |a 9781493991303 |c Online |9 978-1-4939-9130-3 | ||
| 024 | 7 | |a 10.1007/978-1-4939-9130-3 |2 doi | |
| 035 | |a (ZDB-2-PRO)978-1-4939-9130-3 | ||
| 035 | |a (OCoLC)1289768529 | ||
| 035 | |a (DE-599)BVBBV047639738 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-355 |a DE-91 | ||
| 082 | 0 | |a 572.696 | |
| 245 | 1 | 0 | |a Lipid-activated nuclear receptors |b methods and protocols |c edited by Matthew C. Gage, Inés Pineda-Torra |
| 250 | |a 1st ed. 2019 | ||
| 264 | 1 | |a New York, NY |b Springer New York |c 2019 | |
| 300 | |a 1 Online-Ressource (XI, 220 Seiten) |b Illustrationen | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b c |2 rdamedia | ||
| 338 | |b cr |2 rdacarrier | ||
| 490 | 0 | |a Methods in Molecular Biology | |
| 490 | 0 | |a 1951 | |
| 500 | |a Quantification of Oxysterol Nuclear Receptor Ligands by LC/MS/MS -- A Stable Luciferase Reporter System to Characterise LXR Regulation by Oxysterols and Novel Ligands -- Isolation and Analysis of Human Monocytes and Adipose Tissue Macrophages -- Glucan Encapsulated siRNA Particles (GeRPs) for Specific Gene Silencing in Adipose Tissue Macrophages -- Isolation and Purification of Tissue Resident Macrophages for the Analysis of Nuclear Receptor Activity -- Bone Marrow-Derived Macrophage Immortalization of LXR Nuclear Receptor-Deficient Cells -- Dual Crosslinking Chromatin Immunoprecipitation Protocol for Next Generation Sequencing (ChIPseq) in Macrophages -- Analysis of LXR Nuclear Receptor Cistrome through ChIP-seq Data Bioinformatics -- Quantifying Cellular Cholesterol Efflux -- Methods for Assessing the Effects of LXR Agonists on Macrophage Bacterial Infection -- Measuring Apoptotic Cell Engulfment (Efferocytosis) Efficiency -- Methods to Study Monocyte and Macrophage Trafficking in Atherosclerosis Progression and Resolution -- Preparation of Frozen Liver Tissues for Integrated Omics Analysis -- Identification of Nuclear Receptor Targets By Chromatin Immunoprecipitation in Fatty Liver -- The LPS/D-Galactosamine-Induced Fulminant Hepatitis Model to Assess the Role of Ligand-Activated Nuclear Receptors on the NLRP3 Inflammasome Pathway In Vivo -- Analyzing T-Cell Plasma Membrane Lipids by Flow Cytometry | ||
| 520 | |a This book covers a wide range of state-of-the-art methodologies and detailed protocols currently used to study the actions that lipid-activated nuclear receptors and their co-regulators have in tissues and immune cell types considered classic metabolic "powerhouses". This includes the liver, adipose tissue, and monocytes/macrophages present in these and other metabolic tissues. While the main focus is on the oxysterol receptor or Liver X Receptor (LXR), the majority of the methods described can be easily applied to multiple nuclear receptors, as well as to other tissues or cell types. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Lipid-Activated Nuclear Receptors: Methods and Protocols serves as an ideal guide for researchers pursuing the vital study of nuclear receptor biology and beyond | ||
| 650 | 4 | |a Proteins | |
| 650 | 4 | |a Lipids | |
| 700 | 1 | |a Gage, Matthew C. |0 (DE-588)1183961146 |4 edt | |
| 700 | 1 | |a Pineda-Torra, Inés |0 (DE-588)1183961332 |4 edt | |
| 776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |z 978-1-4939-9131-0 |
| 856 | 4 | 0 | |u https://doi.org/10.1007/978-1-4939-9130-3 |x Verlag |z URL des Erstveröffentlichers |3 Volltext |
| 912 | |a ZDB-2-PRO | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-033023949 | ||
| 966 | e | |u https://doi.org/10.1007/978-1-4939-9130-3 |l UBR01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
| 966 | e | |u https://doi.org/10.1007/978-1-4939-9130-3 |l TUM01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
Datensatz im Suchindex
| _version_ | 1804183092262338560 |
|---|---|
| adam_txt | |
| any_adam_object | |
| any_adam_object_boolean | |
| author2 | Gage, Matthew C. Pineda-Torra, Inés |
| author2_role | edt edt |
| author2_variant | m c g mc mcg i p t ipt |
| author_GND | (DE-588)1183961146 (DE-588)1183961332 |
| author_facet | Gage, Matthew C. Pineda-Torra, Inés |
| building | Verbundindex |
| bvnumber | BV047639738 |
| collection | ZDB-2-PRO |
| ctrlnum | (ZDB-2-PRO)978-1-4939-9130-3 (OCoLC)1289768529 (DE-599)BVBBV047639738 |
| dewey-full | 572.696 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.696 |
| dewey-search | 572.696 |
| dewey-sort | 3572.696 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| discipline_str_mv | Biologie |
| doi_str_mv | 10.1007/978-1-4939-9130-3 |
| edition | 1st ed. 2019 |
| format | Electronic eBook |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>04088nmm a2200445zc 4500</leader><controlfield tag="001">BV047639738</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">cr|uuu---uuuuu</controlfield><controlfield tag="008">211214s2019 |||| o||u| ||||||eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781493991303</subfield><subfield code="c">Online</subfield><subfield code="9">978-1-4939-9130-3</subfield></datafield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/978-1-4939-9130-3</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ZDB-2-PRO)978-1-4939-9130-3</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)1289768529</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV047639738</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield><subfield code="a">DE-91</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">572.696</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Lipid-activated nuclear receptors</subfield><subfield code="b">methods and protocols</subfield><subfield code="c">edited by Matthew C. Gage, Inés Pineda-Torra</subfield></datafield><datafield tag="250" ind1=" " ind2=" "><subfield code="a">1st ed. 2019</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">New York, NY</subfield><subfield code="b">Springer New York</subfield><subfield code="c">2019</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">1 Online-Ressource (XI, 220 Seiten)</subfield><subfield code="b">Illustrationen</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Methods in Molecular Biology</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">1951</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Quantification of Oxysterol Nuclear Receptor Ligands by LC/MS/MS -- A Stable Luciferase Reporter System to Characterise LXR Regulation by Oxysterols and Novel Ligands -- Isolation and Analysis of Human Monocytes and Adipose Tissue Macrophages -- Glucan Encapsulated siRNA Particles (GeRPs) for Specific Gene Silencing in Adipose Tissue Macrophages -- Isolation and Purification of Tissue Resident Macrophages for the Analysis of Nuclear Receptor Activity -- Bone Marrow-Derived Macrophage Immortalization of LXR Nuclear Receptor-Deficient Cells -- Dual Crosslinking Chromatin Immunoprecipitation Protocol for Next Generation Sequencing (ChIPseq) in Macrophages -- Analysis of LXR Nuclear Receptor Cistrome through ChIP-seq Data Bioinformatics -- Quantifying Cellular Cholesterol Efflux -- Methods for Assessing the Effects of LXR Agonists on Macrophage Bacterial Infection -- Measuring Apoptotic Cell Engulfment (Efferocytosis) Efficiency -- Methods to Study Monocyte and Macrophage Trafficking in Atherosclerosis Progression and Resolution -- Preparation of Frozen Liver Tissues for Integrated Omics Analysis -- Identification of Nuclear Receptor Targets By Chromatin Immunoprecipitation in Fatty Liver -- The LPS/D-Galactosamine-Induced Fulminant Hepatitis Model to Assess the Role of Ligand-Activated Nuclear Receptors on the NLRP3 Inflammasome Pathway In Vivo -- Analyzing T-Cell Plasma Membrane Lipids by Flow Cytometry</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">This book covers a wide range of state-of-the-art methodologies and detailed protocols currently used to study the actions that lipid-activated nuclear receptors and their co-regulators have in tissues and immune cell types considered classic metabolic "powerhouses". This includes the liver, adipose tissue, and monocytes/macrophages present in these and other metabolic tissues. While the main focus is on the oxysterol receptor or Liver X Receptor (LXR), the majority of the methods described can be easily applied to multiple nuclear receptors, as well as to other tissues or cell types. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Lipid-Activated Nuclear Receptors: Methods and Protocols serves as an ideal guide for researchers pursuing the vital study of nuclear receptor biology and beyond</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Proteins </subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Lipids</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Gage, Matthew C.</subfield><subfield code="0">(DE-588)1183961146</subfield><subfield code="4">edt</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Pineda-Torra, Inés</subfield><subfield code="0">(DE-588)1183961332</subfield><subfield code="4">edt</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Druck-Ausgabe</subfield><subfield code="z">978-1-4939-9131-0</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1007/978-1-4939-9130-3</subfield><subfield code="x">Verlag</subfield><subfield code="z">URL des Erstveröffentlichers</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-2-PRO</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-033023949</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1007/978-1-4939-9130-3</subfield><subfield code="l">UBR01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1007/978-1-4939-9130-3</subfield><subfield code="l">TUM01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield></record></collection> |
| id | DE-604.BV047639738 |
| illustrated | Not Illustrated |
| index_date | 2024-07-03T18:47:37Z |
| indexdate | 2024-07-10T09:17:57Z |
| institution | BVB |
| isbn | 9781493991303 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-033023949 |
| oclc_num | 1289768529 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-91 DE-BY-TUM |
| owner_facet | DE-355 DE-BY-UBR DE-91 DE-BY-TUM |
| physical | 1 Online-Ressource (XI, 220 Seiten) Illustrationen |
| psigel | ZDB-2-PRO |
| publishDate | 2019 |
| publishDateSearch | 2019 |
| publishDateSort | 2019 |
| publisher | Springer New York |
| record_format | marc |
| series2 | Methods in Molecular Biology 1951 |
| spelling | Lipid-activated nuclear receptors methods and protocols edited by Matthew C. Gage, Inés Pineda-Torra 1st ed. 2019 New York, NY Springer New York 2019 1 Online-Ressource (XI, 220 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1951 Quantification of Oxysterol Nuclear Receptor Ligands by LC/MS/MS -- A Stable Luciferase Reporter System to Characterise LXR Regulation by Oxysterols and Novel Ligands -- Isolation and Analysis of Human Monocytes and Adipose Tissue Macrophages -- Glucan Encapsulated siRNA Particles (GeRPs) for Specific Gene Silencing in Adipose Tissue Macrophages -- Isolation and Purification of Tissue Resident Macrophages for the Analysis of Nuclear Receptor Activity -- Bone Marrow-Derived Macrophage Immortalization of LXR Nuclear Receptor-Deficient Cells -- Dual Crosslinking Chromatin Immunoprecipitation Protocol for Next Generation Sequencing (ChIPseq) in Macrophages -- Analysis of LXR Nuclear Receptor Cistrome through ChIP-seq Data Bioinformatics -- Quantifying Cellular Cholesterol Efflux -- Methods for Assessing the Effects of LXR Agonists on Macrophage Bacterial Infection -- Measuring Apoptotic Cell Engulfment (Efferocytosis) Efficiency -- Methods to Study Monocyte and Macrophage Trafficking in Atherosclerosis Progression and Resolution -- Preparation of Frozen Liver Tissues for Integrated Omics Analysis -- Identification of Nuclear Receptor Targets By Chromatin Immunoprecipitation in Fatty Liver -- The LPS/D-Galactosamine-Induced Fulminant Hepatitis Model to Assess the Role of Ligand-Activated Nuclear Receptors on the NLRP3 Inflammasome Pathway In Vivo -- Analyzing T-Cell Plasma Membrane Lipids by Flow Cytometry This book covers a wide range of state-of-the-art methodologies and detailed protocols currently used to study the actions that lipid-activated nuclear receptors and their co-regulators have in tissues and immune cell types considered classic metabolic "powerhouses". This includes the liver, adipose tissue, and monocytes/macrophages present in these and other metabolic tissues. While the main focus is on the oxysterol receptor or Liver X Receptor (LXR), the majority of the methods described can be easily applied to multiple nuclear receptors, as well as to other tissues or cell types. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Lipid-Activated Nuclear Receptors: Methods and Protocols serves as an ideal guide for researchers pursuing the vital study of nuclear receptor biology and beyond Proteins Lipids Gage, Matthew C. (DE-588)1183961146 edt Pineda-Torra, Inés (DE-588)1183961332 edt Erscheint auch als Druck-Ausgabe 978-1-4939-9131-0 https://doi.org/10.1007/978-1-4939-9130-3 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Lipid-activated nuclear receptors methods and protocols Proteins Lipids |
| title | Lipid-activated nuclear receptors methods and protocols |
| title_auth | Lipid-activated nuclear receptors methods and protocols |
| title_exact_search | Lipid-activated nuclear receptors methods and protocols |
| title_exact_search_txtP | Lipid-activated nuclear receptors methods and protocols |
| title_full | Lipid-activated nuclear receptors methods and protocols edited by Matthew C. Gage, Inés Pineda-Torra |
| title_fullStr | Lipid-activated nuclear receptors methods and protocols edited by Matthew C. Gage, Inés Pineda-Torra |
| title_full_unstemmed | Lipid-activated nuclear receptors methods and protocols edited by Matthew C. Gage, Inés Pineda-Torra |
| title_short | Lipid-activated nuclear receptors |
| title_sort | lipid activated nuclear receptors methods and protocols |
| title_sub | methods and protocols |
| topic | Proteins Lipids |
| topic_facet | Proteins Lipids |
| url | https://doi.org/10.1007/978-1-4939-9130-3 |
| work_keys_str_mv | AT gagematthewc lipidactivatednuclearreceptorsmethodsandprotocols AT pinedatorraines lipidactivatednuclearreceptorsmethodsandprotocols |