Alpha-synuclein methods and protocols - Bartels, Tim 1980- (Springer New York, 2019)
Assays for Neuronal Defects Caused by Early Formation of α-Synuclein Inclusions in Primary Cultured Neurons – Immunohistochemical Detection of Alpha-Synuclein in Unfixed Human Brain Tissue – Kinetic Measurements of Endocytosis and Exocytosis in Cultured Neurons – Detection of Misfolded α-Synuclein Aggregates in Cerebrospinal Fluid by the Protein Misfolding Cyclic Amplification Platform – Stereotaxic Targeting of Alpha-Synuclein Pathology in Mouse Brain using Pre-Formed Fibrils – Quantification of Alpha-Synuclein in Biological Fluids by Electrochemiluminescence-Based Detection – Alpha-Synuclein Proximity Ligation Assay (AS-PLA) in Brain Sections to Probe for Alpha-Synuclein Oligomers – Studying α-Synuclein Conformation by Intact-Cell Crosslinking – C. elegans as a Model for Synucleinopathies and Other Neurodegenerative Diseases: Tools and Techniques – Recombinant Expression and Purification of N-Acetylated Alpha-Synuclein – Circular Dichroism and Isothermal Titration Calorimetry to Study the Interaction of α-Synuclein with Membranes – Yeast-Based Screens to Target Alpha-Synuclein Toxicity – Probing Structural Changes in Alpha Synuclein by Nuclear Magnetic Resonance Spectroscopy – Measuring Mitochondrial Dysfunction Caused by Soluble α-Synuclein Oligomers – Monitoring α-Synuclein Proteotoxicity in Drosophila Models – Detection of α-Synuclein in Biological Samples using Mass Spectrometry – Single-Molecule FRET Detection of Early-Stage Conformations in α-Synuclein Aggregation – In Situ Peroxidase Labelling and Mass-Spectrometry of Alpha-Synuclein in Rat Cortical Neurons – Probing Alpha-Synuclein Conformations by Electron Paramagnetic Resonance (EPR) Spectroscopy – Expression and Purification of Untagged α-Synuclein – Generation and Analysis of Viral Vector Mediated Rodent Models for Parkinson's Disease