Kidney organogenesis: methods and protocols
This book details protocols on classical methods and very modern ex vivo mammalian developmental kidney model systems. Chapters focus on generation of mosaic embryonic kidneys, culture of the kidney tissues ex vivo, embryonic kidney progenitor cell, 3Dkidney model cell line based cultures, programmi...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2019
|
| Ausgabe: | 1st ed. 2019 |
| Schriftenreihe: | Methods in Molecular Biology
1926 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | This book details protocols on classical methods and very modern ex vivo mammalian developmental kidney model systems. Chapters focus on generation of mosaic embryonic kidneys, culture of the kidney tissues ex vivo, embryonic kidney progenitor cell, 3Dkidney model cell line based cultures, programming the kidney cell fates in human pluripotent cells, protocols to target the functional players in kidney ontogenesis, 4D and 3D ex vivo imaging, drosphila, and computational approaches to kidney ontogenesis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Kidney Organogenesis: Methods and Protocols aims to ensure successful results in the further study of this vital field |
| Beschreibung: | Generating Genetic Mosaic Mouse Embryos or Organoids for Studies of Kidney Development -- Mouse Ex Vivo Kidney Culture Methods -- Serum-Free Organ Culture of the Embryonic Mouse Ureter -- Experimental Tubulogenesis Induction Model in the Mouse -- Dissociation of Tissues for Single Cell Analysis -- Long Term Culture of Nephron Progenitor Cells Ex Vivo -- 3D Cell Culture Models of Epithelial Tissues -- Generation of Three-Dimensional Nephrons from Mouse and Human Pluripotent Stem Cells -- Generating Multiple Kidney Progenitors and Cell Types from Human Pluripotent Stem Cells -- Protocol to Generate Ureteric Bud Structures from Human iPS Cells -- Investigating Aspects of Renal Physiology and Pharmacology in Organ and Organoid Culture -- Virus as Renal Functional Genetic Tools -- Gene-Editing in 3D Cultured Nephron Progenitor Cell Lines -- Time-Lapse Technologies and 4D Imaging of Kidney Development -- Decellularizing and Recellularizing Adult Mouse Kidneys -- Optical Projection Tomography Imaging to Study Kidney Organogenesis -- Epithelial Function in the Drosophila Malpighian Tubule: An In Vivo Renal Model -- Analysis of the Teashirt Target Genes in Ureteric Bud Development -- Methods of Computational Analysis in Kidney Development |
| Beschreibung: | 1 Online-Ressource (XI, 248 Seiten) Illustrationen |
| ISBN: | 9781493990214 |
| DOI: | 10.1007/978-1-4939-9021-4 |
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| 520 | |a This book details protocols on classical methods and very modern ex vivo mammalian developmental kidney model systems. Chapters focus on generation of mosaic embryonic kidneys, culture of the kidney tissues ex vivo, embryonic kidney progenitor cell, 3Dkidney model cell line based cultures, programming the kidney cell fates in human pluripotent cells, protocols to target the functional players in kidney ontogenesis, 4D and 3D ex vivo imaging, drosphila, and computational approaches to kidney ontogenesis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Kidney Organogenesis: Methods and Protocols aims to ensure successful results in the further study of this vital field | ||
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| dewey-full | 611.01816 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 611 - Human anatomy, cytology, histology |
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| dewey-search | 611.01816 |
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| dewey-tens | 610 - Medicine and health |
| discipline | Medizin |
| discipline_str_mv | Medizin |
| doi_str_mv | 10.1007/978-1-4939-9021-4 |
| edition | 1st ed. 2019 |
| format | Electronic eBook |
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| id | DE-604.BV047639604 |
| illustrated | Not Illustrated |
| index_date | 2024-07-03T18:47:35Z |
| indexdate | 2024-07-10T09:17:56Z |
| institution | BVB |
| isbn | 9781493990214 |
| language | English |
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| physical | 1 Online-Ressource (XI, 248 Seiten) Illustrationen |
| psigel | ZDB-2-PRO |
| publishDate | 2019 |
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| publisher | Springer New York |
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| spelling | Kidney organogenesis methods and protocols edited by Seppo Vainio 1st ed. 2019 New York, NY Springer New York 2019 1 Online-Ressource (XI, 248 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1926 Generating Genetic Mosaic Mouse Embryos or Organoids for Studies of Kidney Development -- Mouse Ex Vivo Kidney Culture Methods -- Serum-Free Organ Culture of the Embryonic Mouse Ureter -- Experimental Tubulogenesis Induction Model in the Mouse -- Dissociation of Tissues for Single Cell Analysis -- Long Term Culture of Nephron Progenitor Cells Ex Vivo -- 3D Cell Culture Models of Epithelial Tissues -- Generation of Three-Dimensional Nephrons from Mouse and Human Pluripotent Stem Cells -- Generating Multiple Kidney Progenitors and Cell Types from Human Pluripotent Stem Cells -- Protocol to Generate Ureteric Bud Structures from Human iPS Cells -- Investigating Aspects of Renal Physiology and Pharmacology in Organ and Organoid Culture -- Virus as Renal Functional Genetic Tools -- Gene-Editing in 3D Cultured Nephron Progenitor Cell Lines -- Time-Lapse Technologies and 4D Imaging of Kidney Development -- Decellularizing and Recellularizing Adult Mouse Kidneys -- Optical Projection Tomography Imaging to Study Kidney Organogenesis -- Epithelial Function in the Drosophila Malpighian Tubule: An In Vivo Renal Model -- Analysis of the Teashirt Target Genes in Ureteric Bud Development -- Methods of Computational Analysis in Kidney Development This book details protocols on classical methods and very modern ex vivo mammalian developmental kidney model systems. Chapters focus on generation of mosaic embryonic kidneys, culture of the kidney tissues ex vivo, embryonic kidney progenitor cell, 3Dkidney model cell line based cultures, programming the kidney cell fates in human pluripotent cells, protocols to target the functional players in kidney ontogenesis, 4D and 3D ex vivo imaging, drosphila, and computational approaches to kidney ontogenesis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Kidney Organogenesis: Methods and Protocols aims to ensure successful results in the further study of this vital field Molecular biology Cell culture Nephrology Vainio, Seppo (DE-588)1208880349 edt Erscheint auch als Druck-Ausgabe 978-1-4939-9022-1 https://doi.org/10.1007/978-1-4939-9021-4 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Kidney organogenesis methods and protocols Molecular biology Cell culture Nephrology |
| title | Kidney organogenesis methods and protocols |
| title_auth | Kidney organogenesis methods and protocols |
| title_exact_search | Kidney organogenesis methods and protocols |
| title_exact_search_txtP | Kidney organogenesis methods and protocols |
| title_full | Kidney organogenesis methods and protocols edited by Seppo Vainio |
| title_fullStr | Kidney organogenesis methods and protocols edited by Seppo Vainio |
| title_full_unstemmed | Kidney organogenesis methods and protocols edited by Seppo Vainio |
| title_short | Kidney organogenesis |
| title_sort | kidney organogenesis methods and protocols |
| title_sub | methods and protocols |
| topic | Molecular biology Cell culture Nephrology |
| topic_facet | Molecular biology Cell culture Nephrology |
| url | https://doi.org/10.1007/978-1-4939-9021-4 |
| work_keys_str_mv | AT vainioseppo kidneyorganogenesismethodsandprotocols |