Immune checkpoint blockade methods and protocols - Pico de Coaña, Yago 1975- (Springer New York, 2019)
Double Immunohistochemistry and Digital Image Analysis -- Multispectral Fluorescence Imaging Allows for Distinctive Topographic Assessment and Sub-Classification of Tumor Infiltrating and Surrounding Immune Cells -- Systems Level Immune Monitoring by Mass Cytometry -- Immune Monitoring of Cancer Patients by Multi-Color Flow Cytometry -- High-Throughput, Fast and Sensitive Immunopeptidomics Sample Processing for Mass Spectrometry -- Identification of Immune Modulatory miRNAs by miRNA Enrichment via RNA Affinity Purification -- Expansion of Tumor-Infiltrating Lymphocytes from Melanoma Tumors -- Establishment of Bi-Dimensional (2D) and Three-Dimensional (3D) Melanoma Primary Cultures as a Tool for In Vitro Drug Resistance Studies -- Assessment of Anti-Tumor T Cell Responses by Flow Cytometry after Co-Culture of Tumor Cells with Autologous Tumor-Infiltrating Lymphocytes -- Expansion and Determination of Antigen-Reactive T Cells by Flow Cytometry -- Assessing Canonical and Adaptive Natural Killer Cell Function in Suppression Assays In Vitro -- Evaluating Antibody-Dependent Cell-Mediated Cytotoxicity by Chromium Release Assay -- Evaluating Antibody-Dependent Cell-Mediated Cytotoxicity by Flow Cytometry -- Evaluation of Breast Cancer and Melanoma Metastasis in Syngeneic Mouse Models -- Establishment of Melanoma Tumor Xenograft Using Single Cell Line Suspension and Co-Injection of Patient-Derived T Cells in Immune Deficient NSG Mice -- Analysis of Tissue Resident Immune Cells from Mouse Skin and Lungs by Flow Cytometry