T-cell motility: methods and protocols
This volume discusses the latest developments in cellular, molecular, biochemical, and imaging assays to study the biology and functions of T-cells. The chapters in this book cover topics such as LFA-1/ICAM-1 interactions in T-cell motility; using 3D-SIM to dissect signaling cross-talks in motile T-...
Saved in:
| Other Authors: | |
|---|---|
| Format: | Electronic eBook |
| Language: | English |
| Published: |
New York, NY
Springer New York
2019
|
| Edition: | 1st ed. 2019 |
| Series: | Methods in Molecular Biology
1930 |
| Subjects: | |
| Online Access: | UBR01 TUM01 Volltext |
| Summary: | This volume discusses the latest developments in cellular, molecular, biochemical, and imaging assays to study the biology and functions of T-cells. The chapters in this book cover topics such as LFA-1/ICAM-1 interactions in T-cell motility; using 3D-SIM to dissect signaling cross-talks in motile T-cells; GapmeR-mediated gene silencing in motile T-cells; activity of cellular kinases in migrating T-cells; and computational analysis of protein-protein interactions in motile T-cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, T-Cell Motility: Methods and Protocols is an essential resource for graduate students, postdoctoral fellows, and principal investigators working in the fields of immunology, T-cell biology, biochemistry, molecular biology, and imaging |
| Item Description: | An Introduction to LFA-1/ICAM-1 Interactions in T-Cell Motility -- Isolation of Human Peripheral Blood T Lymphocytes -- A Laboratory Model to Study T-Cell Motility -- Quantification of T-Cell Migratory Phenotypes using High Content Analysis -- Live Cell Imaging and Analysis to Capture T-Cell Motility in Real-Time -- Three-Dimensional Structured Illumination Microscopy (3D-SIM) to Dissect Signalling Cross-Talks in Motile T-Cells -- Real-Time Impedance-Based Detection of LFA-1-Stimulated T-Cell Transwell Chemotaxis -- Quantitative Real-Time PCR for Evaluating Transcriptional Changes in T-Lymphocytes -- GapmeR-Mediated Gene Silencing in Motile T-Cells -- Live Imaging of Resident T-Cell Migration in Human Lymphoid Tissue Slices using Confocal Microscopy -- Phosphoprotein Enrichment for Protein Analysis in Motile T-Lymphocytes -- Immunometabolomic Phenotyping of Motile T-Cells -- Profiling Activity of Cellular Kinases in Migrating T-Cells -- Biotinylation Assay to Determine LFA-1 Recycling in Motile T-Lymphocytes -- A Protocol to Study T-Cell Signalling in an Immune Synapse by Microscopy -- Enzyme-Linked Immunosorbent Assay for T-Cell Dependent Immunogenicity Assessment of Therapeutic Peptides -- Modified Intravital Microscopy to Assess Vascular Health and T-Cell Motility -- Computational Analysis of Protein-Protein Interactions in Motile T-Cells |
| Physical Description: | 1 Online-Ressource (X, 158 Seiten) Illustrationen |
| ISBN: | 9781493990368 |
| DOI: | 10.1007/978-1-4939-9036-8 |
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| isbn | 9781493990368 |
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| spelling | T-cell motility methods and protocols edited by Navin Kumar Verma 1st ed. 2019 New York, NY Springer New York 2019 1 Online-Ressource (X, 158 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1930 An Introduction to LFA-1/ICAM-1 Interactions in T-Cell Motility -- Isolation of Human Peripheral Blood T Lymphocytes -- A Laboratory Model to Study T-Cell Motility -- Quantification of T-Cell Migratory Phenotypes using High Content Analysis -- Live Cell Imaging and Analysis to Capture T-Cell Motility in Real-Time -- Three-Dimensional Structured Illumination Microscopy (3D-SIM) to Dissect Signalling Cross-Talks in Motile T-Cells -- Real-Time Impedance-Based Detection of LFA-1-Stimulated T-Cell Transwell Chemotaxis -- Quantitative Real-Time PCR for Evaluating Transcriptional Changes in T-Lymphocytes -- GapmeR-Mediated Gene Silencing in Motile T-Cells -- Live Imaging of Resident T-Cell Migration in Human Lymphoid Tissue Slices using Confocal Microscopy -- Phosphoprotein Enrichment for Protein Analysis in Motile T-Lymphocytes -- Immunometabolomic Phenotyping of Motile T-Cells -- Profiling Activity of Cellular Kinases in Migrating T-Cells -- Biotinylation Assay to Determine LFA-1 Recycling in Motile T-Lymphocytes -- A Protocol to Study T-Cell Signalling in an Immune Synapse by Microscopy -- Enzyme-Linked Immunosorbent Assay for T-Cell Dependent Immunogenicity Assessment of Therapeutic Peptides -- Modified Intravital Microscopy to Assess Vascular Health and T-Cell Motility -- Computational Analysis of Protein-Protein Interactions in Motile T-Cells This volume discusses the latest developments in cellular, molecular, biochemical, and imaging assays to study the biology and functions of T-cells. The chapters in this book cover topics such as LFA-1/ICAM-1 interactions in T-cell motility; using 3D-SIM to dissect signaling cross-talks in motile T-cells; GapmeR-mediated gene silencing in motile T-cells; activity of cellular kinases in migrating T-cells; and computational analysis of protein-protein interactions in motile T-cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, T-Cell Motility: Methods and Protocols is an essential resource for graduate students, postdoctoral fellows, and principal investigators working in the fields of immunology, T-cell biology, biochemistry, molecular biology, and imaging Immunology Cell biology Verma, Navin Kumar ca. 20./21. Jh. (DE-588)1247568504 edt Erscheint auch als Druck-Ausgabe 978-1-4939-9037-5 https://doi.org/10.1007/978-1-4939-9036-8 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | T-cell motility methods and protocols Immunology Cell biology |
| title | T-cell motility methods and protocols |
| title_auth | T-cell motility methods and protocols |
| title_exact_search | T-cell motility methods and protocols |
| title_exact_search_txtP | T-cell motility methods and protocols |
| title_full | T-cell motility methods and protocols edited by Navin Kumar Verma |
| title_fullStr | T-cell motility methods and protocols edited by Navin Kumar Verma |
| title_full_unstemmed | T-cell motility methods and protocols edited by Navin Kumar Verma |
| title_short | T-cell motility |
| title_sort | t cell motility methods and protocols |
| title_sub | methods and protocols |
| topic | Immunology Cell biology |
| topic_facet | Immunology Cell biology |
| url | https://doi.org/10.1007/978-1-4939-9036-8 |
| work_keys_str_mv | AT vermanavinkumar tcellmotilitymethodsandprotocols |