T-cell motility methods and protocols - Verma, Navin Kumar ca. 20./21. Jh (Springer New York, 2019)
An Introduction to LFA-1/ICAM-1 Interactions in T-Cell Motility -- Isolation of Human Peripheral Blood T Lymphocytes -- A Laboratory Model to Study T-Cell Motility -- Quantification of T-Cell Migratory Phenotypes using High Content Analysis -- Live Cell Imaging and Analysis to Capture T-Cell Motility in Real-Time -- Three-Dimensional Structured Illumination Microscopy (3D-SIM) to Dissect Signalling Cross-Talks in Motile T-Cells -- Real-Time Impedance-Based Detection of LFA-1-Stimulated T-Cell Transwell Chemotaxis -- Quantitative Real-Time PCR for Evaluating Transcriptional Changes in T-Lymphocytes -- GapmeR-Mediated Gene Silencing in Motile T-Cells -- Live Imaging of Resident T-Cell Migration in Human Lymphoid Tissue Slices using Confocal Microscopy -- Phosphoprotein Enrichment for Protein Analysis in Motile T-Lymphocytes -- Immunometabolomic Phenotyping of Motile T-Cells -- Profiling Activity of Cellular Kinases in Migrating T-Cells -- Biotinylation Assay to Determine LFA-1 Recycling in Motile T-Lymphocytes -- A Protocol to Study T-Cell Signalling in an Immune Synapse by Microscopy -- Enzyme-Linked Immunosorbent Assay for T-Cell Dependent Immunogenicity Assessment of Therapeutic Peptides -- Modified Intravital Microscopy to Assess Vascular Health and T-Cell Motility -- Computational Analysis of Protein-Protein Interactions in Motile T-Cells